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The map label for this gene is iolD [H]

Identifier: 186476359

GI number: 186476359

Start: 1818241

End: 1820190

Strand: Direct

Name: iolD [H]

Synonym: Bphy_1601

Alternate gene names: 186476359

Gene position: 1818241-1820190 (Clockwise)

Preceding gene: 186476358

Following gene: 186476360

Centisome position: 52.26

GC content: 66.1

Gene sequence:

>1950_bases
ATGAACCAGCGCGCGTTGCATCATGATGTGACGACTCCATCCGACACGATCGCGCAGGTATGCGCCGATGGCACGATCCG
TCTGACGGCGGCGCAGGCCGTCGTCCGTTATCTCGCCGCACAGCTCGTCGAGACGGAAGACGGCACGGGCACCGAGCCGC
TATTCGGCGGCGTGTTCGCGATCTTCGGGCATGGCAATGTGGCGGGCCTCGGCGAAGCGCTCTATCAATATCGTCACGAA
TTGCCGACTTACCGTGCGCACAACGAACAGGCGATGGCGCACAGCGCGATCGCCTTCGCCAAGGCGCACTTCCGGCGGCG
CATGATGGCGGTGACGACATCGATCGGTCCGGGCGCAACCAATCTCGTGACGGCGGCGGCGCTCGCGCATGTGAACCGCT
TGCCCGTGCTGCTGCTGCCTGGCGATATCTTTGTGTCGCGCGCGCCGGACCCCGTGTTGCAGCAGGTGGAAGATTTCCAC
GACGGCGGCATCTCGGCGAACGACGCGTTCAAGCCCGTGTCGCGTTACTTCGATCGCATCGTGCATCCCGCGCAATTGCT
CAACGCGCTGCCGCGCGCGCTGCGCGTGCTGACGGACGCCGCGCTGTGCGGCCCCGTCACGCTCGCACTGCCGCAGGACG
TGCAGGCCACGGCCTACGACTACCCCGCGGATTTCTTCGAACCGCGCATCGTGAAGTTTCATGCGCCCCCGCCCGTCGAG
CACGAACTCGACGATGCGCTGCTCGTGCTGCGCAACGCGAAGCGGCCGATGATCGTAGCAGGTGGCGGCGTGCTCTACGG
CCGCTCCACGGATGCACTGAAGACATTCGCGTCAAAGCACGGCATTCCCGTTGCGGAAACGCAGGCGGGCAAGAGCGCGC
TCGCGTGGGACGACCCGCTGAATCTCGGCTCGCTCGGCGTCACGGGCTCGCCGGGCGCCAATGAGATCGCTCACGAAGCC
GATTGCGTGCTGGCCGTCGGCACGCGTCTGCAGGACTTCACGACGGGTTCGAACACGCTCTTCGCGCAGGCGCAGGTGAT
CGGCATCAATGCGAATGCGTTCGATGGTCTCAAGCATCGCGGCTGTATCGTGCAAGCCGATGCAAAGCTTGCACTCGAAG
CCGTCGGCTCGCGGCTGGAAGGCTGGCAACCGGACAGCGCATGGACCGCGCGCGCGCAACAGCGTGCGAACGAATGGCGC
GACATCGTGCACAGGCTCACGCACGCGCCTCAACACACGGCGGAAGGTCAAAGCGTGCTGCCGTACGACGCCGACGTAAT
CGGCGCCGTGCAGCGTTCCAGCGAACACTCGACCACCGACGACATCGTCGTCTGCGCGGCGGGCACGCTGCCCGCCGAAC
TGCACAAGCTATGGCGCGCAGCACGTCCGGGCGCGTATCACGTCGAGTATGGCTATTCGTGCATGGGCTACGAGATCGCA
GGCGGCTTGGGCGCCAAGCTCGCACGGCCCGAGCGAGAGGTGATCGTGATGGTCGGCGACGGCAGCTATCTGATGATGAA
CAGTGAGATCGCGACCTCCGTGATGCTCGGCGCGAAGCTGATCGTCGTCGTGCTCGACAATCGCGGCTATGGCTGCATCA
ACCGCCTGCAGCAGGCATGCGGCGGCGCGCCGTTCAACAACATGTTCGACGACTGCATGCAGGGCACACCCGGCGCTGCG
CATATCGACTTCGCCGCGCACGCGCGCGCGATGGGCGCGCAGGCCGAGCATGTCGCGAACGTGCAGGAACTCGAAGCCGC
GATGCGGCGCGCGCGCGCCGCCGACCGCACGTATCTGGTGTGCATCGACACCGACGCCGCGCGCACCACGGACCAAGGCG
GCTGGTGGTGGGAAGTCGCCGTGCCTGAAGTATCGCCGCGCGAAGGCGTGCGCCAGGCACGCGCCGATTACGAAGCGCAC
ACGAATGCGCGTGGCAGGAACAACGAGTGA

Upstream 100 bases:

>100_bases
GCGTGCGCCGCACGTTCGAAACACTGATCCACTCGTGGCGTGAGCATCGTGGCGCATCGCATGCGGCAGGCTCGCGTCAC
GTTAATCGGGAGCAGGCGGC

Downstream 100 bases:

>100_bases
GGAAGCGCACATGAGTCAGTTCGAAGTACGCATCGGCATCAATCCGCTGTCGTGGATGAACGACGATCTGCCGTCGCTCG
GCGGCGAGACGCCCCTCGAT

Product: thiamine pyrophosphate protein central region

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MNQRALHHDVTTPSDTIAQVCADGTIRLTAAQAVVRYLAAQLVETEDGTGTEPLFGGVFAIFGHGNVAGLGEALYQYRHE
LPTYRAHNEQAMAHSAIAFAKAHFRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDIFVSRAPDPVLQQVEDFH
DGGISANDAFKPVSRYFDRIVHPAQLLNALPRALRVLTDAALCGPVTLALPQDVQATAYDYPADFFEPRIVKFHAPPPVE
HELDDALLVLRNAKRPMIVAGGGVLYGRSTDALKTFASKHGIPVAETQAGKSALAWDDPLNLGSLGVTGSPGANEIAHEA
DCVLAVGTRLQDFTTGSNTLFAQAQVIGINANAFDGLKHRGCIVQADAKLALEAVGSRLEGWQPDSAWTARAQQRANEWR
DIVHRLTHAPQHTAEGQSVLPYDADVIGAVQRSSEHSTTDDIVVCAAGTLPAELHKLWRAARPGAYHVEYGYSCMGYEIA
GGLGAKLARPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMFDDCMQGTPGAA
HIDFAAHARAMGAQAEHVANVQELEAAMRRARAADRTYLVCIDTDAARTTDQGGWWWEVAVPEVSPREGVRQARADYEAH
TNARGRNNE

Sequences:

>Translated_649_residues
MNQRALHHDVTTPSDTIAQVCADGTIRLTAAQAVVRYLAAQLVETEDGTGTEPLFGGVFAIFGHGNVAGLGEALYQYRHE
LPTYRAHNEQAMAHSAIAFAKAHFRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDIFVSRAPDPVLQQVEDFH
DGGISANDAFKPVSRYFDRIVHPAQLLNALPRALRVLTDAALCGPVTLALPQDVQATAYDYPADFFEPRIVKFHAPPPVE
HELDDALLVLRNAKRPMIVAGGGVLYGRSTDALKTFASKHGIPVAETQAGKSALAWDDPLNLGSLGVTGSPGANEIAHEA
DCVLAVGTRLQDFTTGSNTLFAQAQVIGINANAFDGLKHRGCIVQADAKLALEAVGSRLEGWQPDSAWTARAQQRANEWR
DIVHRLTHAPQHTAEGQSVLPYDADVIGAVQRSSEHSTTDDIVVCAAGTLPAELHKLWRAARPGAYHVEYGYSCMGYEIA
GGLGAKLARPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMFDDCMQGTPGAA
HIDFAAHARAMGAQAEHVANVQELEAAMRRARAADRTYLVCIDTDAARTTDQGGWWWEVAVPEVSPREGVRQARADYEAH
TNARGRNNE
>Mature_649_residues
MNQRALHHDVTTPSDTIAQVCADGTIRLTAAQAVVRYLAAQLVETEDGTGTEPLFGGVFAIFGHGNVAGLGEALYQYRHE
LPTYRAHNEQAMAHSAIAFAKAHFRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDIFVSRAPDPVLQQVEDFH
DGGISANDAFKPVSRYFDRIVHPAQLLNALPRALRVLTDAALCGPVTLALPQDVQATAYDYPADFFEPRIVKFHAPPPVE
HELDDALLVLRNAKRPMIVAGGGVLYGRSTDALKTFASKHGIPVAETQAGKSALAWDDPLNLGSLGVTGSPGANEIAHEA
DCVLAVGTRLQDFTTGSNTLFAQAQVIGINANAFDGLKHRGCIVQADAKLALEAVGSRLEGWQPDSAWTARAQQRANEWR
DIVHRLTHAPQHTAEGQSVLPYDADVIGAVQRSSEHSTTDDIVVCAAGTLPAELHKLWRAARPGAYHVEYGYSCMGYEIA
GGLGAKLARPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMFDDCMQGTPGAA
HIDFAAHARAMGAQAEHVANVQELEAAMRRARAADRTYLVCIDTDAARTTDQGGWWWEVAVPEVSPREGVRQARADYEAH
TNARGRNNE

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=526, Percent_Identity=25.6653992395437, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI87081685, Length=594, Percent_Identity=26.2626262626263, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1786717, Length=505, Percent_Identity=25.9405940594059, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=23.9583333333333, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69781; Mature: 69781

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRALHHDVTTPSDTIAQVCADGTIRLTAAQAVVRYLAAQLVETEDGTGTEPLFGGVFA
CCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHCCEEE
IFGHGNVAGLGEALYQYRHELPTYRAHNEQAMAHSAIAFAKAHFRRRMMAVTTSIGPGAT
EECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
NLVTAAALAHVNRLPVLLLPGDIFVSRAPDPVLQQVEDFHDGGISANDAFKPVSRYFDRI
HHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VHPAQLLNALPRALRVLTDAALCGPVTLALPQDVQATAYDYPADFFEPRIVKFHAPPPVE
CCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHCCCEEEEECCCCCCC
HELDDALLVLRNAKRPMIVAGGGVLYGRSTDALKTFASKHGIPVAETQAGKSALAWDDPL
CCHHHHHHHHHCCCCCEEEECCEEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCC
NLGSLGVTGSPGANEIAHEADCVLAVGTRLQDFTTGSNTLFAQAQVIGINANAFDGLKHR
CCCCCCCCCCCCCHHHHHHCCEEEEECCCHHHCCCCCCEEEEEEEEEEECCCHHCCCCCC
GCIVQADAKLALEAVGSRLEGWQPDSAWTARAQQRANEWRDIVHRLTHAPQHTAEGQSVL
CEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEC
PYDADVIGAVQRSSEHSTTDDIVVCAAGTLPAELHKLWRAARPGAYHVEYGYSCMGYEIA
CCCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCEEECEEEC
GGLGAKLARPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQAC
CCCCCCCCCCCCEEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHH
GGAPFNNMFDDCMQGTPGAAHIDFAAHARAMGAQAEHVANVQELEAAMRRARAADRTYLV
CCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
CIDTDAARTTDQGGWWWEVAVPEVSPREGVRQARADYEAHTNARGRNNE
EEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNQRALHHDVTTPSDTIAQVCADGTIRLTAAQAVVRYLAAQLVETEDGTGTEPLFGGVFA
CCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHCCEEE
IFGHGNVAGLGEALYQYRHELPTYRAHNEQAMAHSAIAFAKAHFRRRMMAVTTSIGPGAT
EECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
NLVTAAALAHVNRLPVLLLPGDIFVSRAPDPVLQQVEDFHDGGISANDAFKPVSRYFDRI
HHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VHPAQLLNALPRALRVLTDAALCGPVTLALPQDVQATAYDYPADFFEPRIVKFHAPPPVE
CCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHCCCEEEEECCCCCCC
HELDDALLVLRNAKRPMIVAGGGVLYGRSTDALKTFASKHGIPVAETQAGKSALAWDDPL
CCHHHHHHHHHCCCCCEEEECCEEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCC
NLGSLGVTGSPGANEIAHEADCVLAVGTRLQDFTTGSNTLFAQAQVIGINANAFDGLKHR
CCCCCCCCCCCCCHHHHHHCCEEEEECCCHHHCCCCCCEEEEEEEEEEECCCHHCCCCCC
GCIVQADAKLALEAVGSRLEGWQPDSAWTARAQQRANEWRDIVHRLTHAPQHTAEGQSVL
CEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEC
PYDADVIGAVQRSSEHSTTDDIVVCAAGTLPAELHKLWRAARPGAYHVEYGYSCMGYEIA
CCCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCEEECEEEC
GGLGAKLARPEREVIVMVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQAC
CCCCCCCCCCCCEEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHH
GGAPFNNMFDDCMQGTPGAAHIDFAAHARAMGAQAEHVANVQELEAAMRRARAADRTYLV
CCCCCHHHHHHHHCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEE
CIDTDAARTTDQGGWWWEVAVPEVSPREGVRQARADYEAHTNARGRNNE
EEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA