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The map label for this gene is tcyA [H]

Identifier: 186476289

GI number: 186476289

Start: 1727941

End: 1728723

Strand: Reverse

Name: tcyA [H]

Synonym: Bphy_1531

Alternate gene names: 186476289

Gene position: 1728723-1727941 (Counterclockwise)

Preceding gene: 186476290

Following gene: 186476288

Centisome position: 49.69

GC content: 61.69

Gene sequence:

>783_bases
ATGAAGTCGATTCGATCCCTTCTGCTGATTGGCCTGCTGCAGGTCGCGACGGCGACGGCCGCGTTCGCCGCCGACGACCT
CGCGAAGATCAAGTCCGCCGGCGCCCTCAAGATCGGCACGGAAGGCACCTACGCGCCGTTCACATACCACGACGCGTCCA
ACCAGCTCACGGGCTTCGACGTCGAGATCGGCCGTGCGATCGCGCAAAAGCTGGGTGTCAAGGCCGAGTTCATCGAAGGC
AAGTGGGACGGCCTTATCGCCGGTCTCGACGCTAACCGCTACGACGCCGTGATCAACGAAGTGGCCGTCACCGACGCGCG
CAAGCAGAAGTACGACTTCTCGGAGCCGTACATCGTGTCGCACGCAGCGCTGATCGTGCGCTCGGACAACAGCTCGATCA
AGGGTTTCGACGACCTGAAGGGCAAGAAGTCGGCCAACACGCTGACCAGCAACTTCGGCAAGATCGCGGCCGCACACGGC
GCAGAAGTGGTCCCTGTGCAGGGCTTCAACGAATCGATCGACCTGCTGACCTCGGGCCGCGTCGACGCAACGGTCAACGA
CTCGCTGTCGTTCCTCGACTTCAAGAAGCACAAGCCGGACGCGAAGGTGAAGATCGCCGCGGTCGAAACGGCATCGGATA
GCTCCGACCACTCGGCCGTGCTGATCCGCAAGGGCAACCCTGAACTGCAGGCCGCCATCAACAAGGCGCTGGCAGAAATC
AAGGCCGACGGTACGTACGCGAAGATCTCCGAGAAGTACTTCGGCAAAGACGTTTCCAAGTAA

Upstream 100 bases:

>100_bases
TACAGTCCGCCGACGCAACCGGACGCATAGTCCGGGAGAACCGGACGCACAGCCCCTGGAACACCGGACAAACACATAAC
GAACCTCGGAGCCCACCCTT

Downstream 100 bases:

>100_bases
GCACGAAGGCGAGCCTGAATCATGCCTGCCTGGTTGCATCTGATGGGTGAATCGCTGTGGCCGCTGCTTTATGCGGGCCT
GGTGTTCACCGTGCCGTTGA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKSIRSLLLIGLLQVATATAAFAADDLAKIKSAGALKIGTEGTYAPFTYHDASNQLTGFDVEIGRAIAQKLGVKAEFIEG
KWDGLIAGLDANRYDAVINEVAVTDARKQKYDFSEPYIVSHAALIVRSDNSSIKGFDDLKGKKSANTLTSNFGKIAAAHG
AEVVPVQGFNESIDLLTSGRVDATVNDSLSFLDFKKHKPDAKVKIAAVETASDSSDHSAVLIRKGNPELQAAINKALAEI
KADGTYAKISEKYFGKDVSK

Sequences:

>Translated_260_residues
MKSIRSLLLIGLLQVATATAAFAADDLAKIKSAGALKIGTEGTYAPFTYHDASNQLTGFDVEIGRAIAQKLGVKAEFIEG
KWDGLIAGLDANRYDAVINEVAVTDARKQKYDFSEPYIVSHAALIVRSDNSSIKGFDDLKGKKSANTLTSNFGKIAAAHG
AEVVPVQGFNESIDLLTSGRVDATVNDSLSFLDFKKHKPDAKVKIAAVETASDSSDHSAVLIRKGNPELQAAINKALAEI
KADGTYAKISEKYFGKDVSK
>Mature_260_residues
MKSIRSLLLIGLLQVATATAAFAADDLAKIKSAGALKIGTEGTYAPFTYHDASNQLTGFDVEIGRAIAQKLGVKAEFIEG
KWDGLIAGLDANRYDAVINEVAVTDARKQKYDFSEPYIVSHAALIVRSDNSSIKGFDDLKGKKSANTLTSNFGKIAAAHG
AEVVPVQGFNESIDLLTSGRVDATVNDSLSFLDFKKHKPDAKVKIAAVETASDSSDHSAVLIRKGNPELQAAINKALAEI
KADGTYAKISEKYFGKDVSK

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=261, Percent_Identity=40.2298850574713, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788648, Length=238, Percent_Identity=32.3529411764706, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788649, Length=261, Percent_Identity=31.8007662835249, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787085, Length=253, Percent_Identity=30.0395256916996, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787088, Length=231, Percent_Identity=30.7359307359307, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787031, Length=257, Percent_Identity=27.6264591439689, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27710; Mature: 27710

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIRSLLLIGLLQVATATAAFAADDLAKIKSAGALKIGTEGTYAPFTYHDASNQLTGFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCCCC
VEIGRAIAQKLGVKAEFIEGKWDGLIAGLDANRYDAVINEVAVTDARKQKYDFSEPYIVS
HHHHHHHHHHHCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
HAALIVRSDNSSIKGFDDLKGKKSANTLTSNFGKIAAAHGAEVVPVQGFNESIDLLTSGR
EEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHEEECCCCEEEEECCCCCCCCEEECCC
VDATVNDSLSFLDFKKHKPDAKVKIAAVETASDSSDHSAVLIRKGNPELQAAINKALAEI
CCEEECCCCCEEEHHCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHH
KADGTYAKISEKYFGKDVSK
HCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSIRSLLLIGLLQVATATAAFAADDLAKIKSAGALKIGTEGTYAPFTYHDASNQLTGFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCCCCCCCCC
VEIGRAIAQKLGVKAEFIEGKWDGLIAGLDANRYDAVINEVAVTDARKQKYDFSEPYIVS
HHHHHHHHHHHCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
HAALIVRSDNSSIKGFDDLKGKKSANTLTSNFGKIAAAHGAEVVPVQGFNESIDLLTSGR
EEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHEEECCCCEEEEECCCCCCCCEEECCC
VDATVNDSLSFLDFKKHKPDAKVKIAAVETASDSSDHSAVLIRKGNPELQAAINKALAEI
CCEEECCCCCEEEHHCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHH
KADGTYAKISEKYFGKDVSK
HCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]