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The map label for this gene is lysS

Identifier: 186476208

GI number: 186476208

Start: 1632938

End: 1634476

Strand: Direct

Name: lysS

Synonym: Bphy_1450

Alternate gene names: 186476208

Gene position: 1632938-1634476 (Clockwise)

Preceding gene: 186476207

Following gene: 186476209

Centisome position: 46.93

GC content: 62.64

Gene sequence:

>1539_bases
ATGACCGAACCGACCCAGCCTAACGCTGCCCCGCAGAACGCAGTCGAACTGGACGACAACCAGATCATCGCTGAGCGCCG
CGAAAAGTTGCGCGCGCTGCGCGAGCAAGGCGTCGCCTATCCGAACGACTTCCGTCCGACCCACCACGCCGCCGATCTGC
AAACGGAATACGCGGACGCCGACAAGGACGCGCTCGAAACCAAAGCGCTGCACGTCGCGCTGGCCGGCCGGATGATGCTC
AAGCGTGTGATGGGCAAGGCGAGCTTCGCAACGGTGCGCGACGGCTCGGGCCAGATCCAGTTTTTCATCACGCCCGCCGA
TGTCGGCGAAGCGACCTACGAAGCATTCAAGAAGTGGGATCTGGGCGACATCGTGGCCGCGAAGGGCGTGCTGTTCCGCA
CCAACAAGGGCGAACTCTCGGTGCGCTGCACGGAACTGCGTCTGCTGTCGAAAGCCCTGCGTCCGCTGCCGGACAAGTTC
CACGGTCTCGCCGACCAGGAAATGCGCTATCGCCAGCGCTATGTCGACCTGATCGTCACGGACGAAGCGCGCAAGACCTT
CGTCGCACGTACGAAGGCCGTGTCGTCGATCCGCAAGTTCATGGCCGAGGCGGACTTCATGGAAGTCGAAACGCCGATGC
TGCACCCGATCCCGGGCGGCGCCGCGGCCAAGCCGTTCGTCACGCATCACAATGCGCTCGACATGCAGATGTTCCTGCGT
ATCGCGCCGGAGCTGTATCTGAAGCGACTGGTGGTCGGCGGCTTCGAGCGCGTGTTCGAAATCAACCGGAATTTCCGTAA
CGAGGGCGTGTCGCCGCGTCACAACCCGGAATTCACGATGATGGAGTTCTACGCTGCGTACACCGACTACAAGTGGCTGA
TGGATTTCACGGAACAACTGATCCGTCAGGCCGCCGTCGACGCGCTCGGCAACGCGGCCATCACGTATCAGGGCCGCGAA
CTCGATCTGTCGAAGCCGTTCCATCGTCTGACAATCACGCAGGCAATCCAGAAATACGCGCCGCAATACTCGGACGCGCA
ACTGGCCGACGGCGCGTTCCTGCGCACCGAACTGAAGAAGTTCGGTGTGGACGCGACACAGCCGGCGTTCCTGAACGCGG
GCATCGGCGCGCTGCAACTGGCGCTGTTCGAAGAAACCGCGGAATCGCAACTCTGGGAGCCGACTTTCATCATCGACTAT
CCGATCGAAGTGTCGCCGCTGGCGCGCGCGTCGGATAGCGTCGACGGCATCACCGAGCGCTTCGAGCTGTTCATTACGGG
CCGCGAGATCGCGAACGGCTTCTCGGAGCTGAACGATCCCGAAGACCAGGCTGCACGCTTCAAGAAGCAGGTCGACCAGA
AAGACGCGGGCGACGAGGAAGCGATGTACTACGACGCCGACTACATCCGCGCGCTCGAGTACGGCATGCCGCCTGCGGGC
GGCTGCGGCATCGGCATCGACCGTCTCGTGATGCTGCTCACGGACAGCCCGAGCATCCGCGACGTTATCCTGTTCCCGCA
TTTGCGTCGCGAAGACTGA

Upstream 100 bases:

>100_bases
GCACCCGGCGCGGCACATTCCGCCGCGCCGCATTTTTTCACCGCCTGTGCGCCCGCATCGTCGAGCGCCCACGAGGCACC
GGACACCGAACATCATCACC

Downstream 100 bases:

>100_bases
TCTCCCGCCGCGTTAAGCGCGGCTTCAATCGCGTGCACGGAGGGTTTGCTGGCGCCGTGCACGGCGCTTCTCCCCTTGCC
CTGCCCCGTTGCCGACCGGT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTEPTQPNAAPQNAVELDDNQIIAERREKLRALREQGVAYPNDFRPTHHAADLQTEYADADKDALETKALHVALAGRMML
KRVMGKASFATVRDGSGQIQFFITPADVGEATYEAFKKWDLGDIVAAKGVLFRTNKGELSVRCTELRLLSKALRPLPDKF
HGLADQEMRYRQRYVDLIVTDEARKTFVARTKAVSSIRKFMAEADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLR
IAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYKWLMDFTEQLIRQAAVDALGNAAITYQGRE
LDLSKPFHRLTITQAIQKYAPQYSDAQLADGAFLRTELKKFGVDATQPAFLNAGIGALQLALFEETAESQLWEPTFIIDY
PIEVSPLARASDSVDGITERFELFITGREIANGFSELNDPEDQAARFKKQVDQKDAGDEEAMYYDADYIRALEYGMPPAG
GCGIGIDRLVMLLTDSPSIRDVILFPHLRRED

Sequences:

>Translated_512_residues
MTEPTQPNAAPQNAVELDDNQIIAERREKLRALREQGVAYPNDFRPTHHAADLQTEYADADKDALETKALHVALAGRMML
KRVMGKASFATVRDGSGQIQFFITPADVGEATYEAFKKWDLGDIVAAKGVLFRTNKGELSVRCTELRLLSKALRPLPDKF
HGLADQEMRYRQRYVDLIVTDEARKTFVARTKAVSSIRKFMAEADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLR
IAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYKWLMDFTEQLIRQAAVDALGNAAITYQGRE
LDLSKPFHRLTITQAIQKYAPQYSDAQLADGAFLRTELKKFGVDATQPAFLNAGIGALQLALFEETAESQLWEPTFIIDY
PIEVSPLARASDSVDGITERFELFITGREIANGFSELNDPEDQAARFKKQVDQKDAGDEEAMYYDADYIRALEYGMPPAG
GCGIGIDRLVMLLTDSPSIRDVILFPHLRRED
>Mature_511_residues
TEPTQPNAAPQNAVELDDNQIIAERREKLRALREQGVAYPNDFRPTHHAADLQTEYADADKDALETKALHVALAGRMMLK
RVMGKASFATVRDGSGQIQFFITPADVGEATYEAFKKWDLGDIVAAKGVLFRTNKGELSVRCTELRLLSKALRPLPDKFH
GLADQEMRYRQRYVDLIVTDEARKTFVARTKAVSSIRKFMAEADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRI
APELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYKWLMDFTEQLIRQAAVDALGNAAITYQGREL
DLSKPFHRLTITQAIQKYAPQYSDAQLADGAFLRTELKKFGVDATQPAFLNAGIGALQLALFEETAESQLWEPTFIIDYP
IEVSPLARASDSVDGITERFELFITGREIANGFSELNDPEDQAARFKKQVDQKDAGDEEAMYYDADYIRALEYGMPPAGG
CGIGIDRLVMLLTDSPSIRDVILFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=509, Percent_Identity=40.6679764243615, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI194272210, Length=509, Percent_Identity=40.6679764243615, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI40789249, Length=326, Percent_Identity=22.3926380368098, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1789256, Length=506, Percent_Identity=58.8932806324111, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1790571, Length=494, Percent_Identity=58.0971659919028, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI87082379, Length=330, Percent_Identity=31.5151515151515, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788173, Length=267, Percent_Identity=26.2172284644195, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=493, Percent_Identity=40.5679513184584, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17535927, Length=493, Percent_Identity=40.5679513184584, Blast_Score=349, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI71994340, Length=493, Percent_Identity=40.5679513184584, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI32566633, Length=299, Percent_Identity=26.4214046822742, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=524, Percent_Identity=40.6488549618321, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324256, Length=548, Percent_Identity=30.1094890510949, Blast_Score=189, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24640849, Length=514, Percent_Identity=40.272373540856, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640851, Length=514, Percent_Identity=40.272373540856, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24584738, Length=343, Percent_Identity=25.0728862973761, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922416, Length=467, Percent_Identity=21.8415417558887, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_BURP8 (B2JIY1)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857678.1
- GeneID:   6242941
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1450
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 57739; Mature: 57608

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPTQPNAAPQNAVELDDNQIIAERREKLRALREQGVAYPNDFRPTHHAADLQTEYADA
CCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCC
DKDALETKALHVALAGRMMLKRVMGKASFATVRDGSGQIQFFITPADVGEATYEAFKKWD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHCCC
LGDIVAAKGVLFRTNKGELSVRCTELRLLSKALRPLPDKFHGLADQEMRYRQRYVDLIVT
CHHHHHHCCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHEEEE
DEARKTFVARTKAVSSIRKFMAEADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLR
CHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHH
IAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYKWLMDFTEQL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IRQAAVDALGNAAITYQGRELDLSKPFHRLTITQAIQKYAPQYSDAQLADGAFLRTELKK
HHHHHHHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
FGVDATQPAFLNAGIGALQLALFEETAESQLWEPTFIIDYPIEVSPLARASDSVDGITER
HCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCHHHHHHH
FELFITGREIANGFSELNDPEDQAARFKKQVDQKDAGDEEAMYYDADYIRALEYGMPPAG
HHEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCCCC
GCGIGIDRLVMLLTDSPSIRDVILFPHLRRED
CCCCCHHHHEEEECCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
TEPTQPNAAPQNAVELDDNQIIAERREKLRALREQGVAYPNDFRPTHHAADLQTEYADA
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCC
DKDALETKALHVALAGRMMLKRVMGKASFATVRDGSGQIQFFITPADVGEATYEAFKKWD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHCCC
LGDIVAAKGVLFRTNKGELSVRCTELRLLSKALRPLPDKFHGLADQEMRYRQRYVDLIVT
CHHHHHHCCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHEEEE
DEARKTFVARTKAVSSIRKFMAEADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLR
CHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHH
IAPELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYKWLMDFTEQL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IRQAAVDALGNAAITYQGRELDLSKPFHRLTITQAIQKYAPQYSDAQLADGAFLRTELKK
HHHHHHHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
FGVDATQPAFLNAGIGALQLALFEETAESQLWEPTFIIDYPIEVSPLARASDSVDGITER
HCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCHHHHHHH
FELFITGREIANGFSELNDPEDQAARFKKQVDQKDAGDEEAMYYDADYIRALEYGMPPAG
HHEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCCCC
GCGIGIDRLVMLLTDSPSIRDVILFPHLRRED
CCCCCHHHHEEEECCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA