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The map label for this gene is prfB [H]

Identifier: 186476207

GI number: 186476207

Start: 1631751

End: 1632830

Strand: Direct

Name: prfB [H]

Synonym: Bphy_1449

Alternate gene names: 186476207

Gene position: 1631751-1632830 (Clockwise)

Preceding gene: 186476206

Following gene: 186476208

Centisome position: 46.9

GC content: 61.02

Gene sequence:

>1080_bases
TTGACTCCTCTCTGGCGGACCTGCGCACACGCGCAGGCGAGCTCCGGGGGTATCTTTGACTACGATGCAAAGTCGGTGCG
ACTGATCGAAGTCAACAAGGAACTCGAAGACCCGAACGTCTGGAATGACTCCCAGCACGCACAGGCGCTCGGCAAGGAAA
AGAAGTTGCTCGAAGGCGTCGTCGACGTGCTGTCGTCGCTCGATAACGACCTGCGCGACACGAAGGACCTCTTCGATATG
GCGCGCGAGGAAAACGACGAGGACACGCTCGTCGCGTGCGAGGAAGACGTCGCAAAGCTGACGACGCGCGTCGAAGACAT
GGAGTTCCGCCGGATGTTCTCGAATCCGGCCGACCCGAACAACGCGTTCATCGACATTCAGGCGGGCGCGGGCGGTACGG
AAGCGTGCGACTGGGCGTCGATGCTGCTGCGCCAGTATCTGCGCTATTGCGAACGCAAGGGCTTCAAGACGGAAGTGCTG
GAAGAGTCCGAAGGCGACGTCGCCGGCATCAAGAGCGCGACGATCAAGGTCGAAGGCGAATACGCGTACGGCTTCCTGCG
CACCGAAACGGGCATTCACCGCCTCGTGCGCAAATCGCCGTTCGACTCGTCGGGCGGCCGTCACACGTCGTTCTCGTCGG
TGTTCGTCTATCCGGAAATCGACGAGTCGTTCGAGATCGAAGTCAATCCGGCCGATCTGCGCATCGACACGTACCGCGCT
TCGGGCGCGGGCGGTCAGCACATCAACAAGACGGACTCCGCCGTGCGTATCACGCACATCCCGTCGGGCATCGTCGTGCA
GTGCCAGAACGACCGCTCGCAGCACCGCAATCGCGCCGAAGCGATGGCAATGCTGAAATCGCGCCTGTACGAAGCCGAAA
TGCGCAAACGCCAGTCGGAGCAGGACAAGCTCGAAGCAGGCAAGTCGGACGTGGGCTGGGGTCATCAAATCCGCTCGTAC
GTGCTCGACAACAGCCGTATCAAGGACCTGCGCACCAACGTCGAAATCAGCAACACGAAGAGCGTGCTCGACGGCGATCT
CGACGCATTCATCAGCGCGAGCCTGAAACAGGGCATCTAA

Upstream 100 bases:

>100_bases
AGCACGAAACCGAAGTCAAAATCGTAACCCTGACTGCGTCGCAGACAAAAACCTTACTTATCTCACTGGATCGACATGGA
AGCGGAACGCCTGAACTCCA

Downstream 100 bases:

>100_bases
GGGCAGCGCACCCGGCGCGGCACATTCCGCCGCGCCGCATTTTTTCACCGCCTGTGCGCCCGCATCGTCGAGCGCCCACG
AGGCACCGGACACCGAACAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTPLWRTCAHAQASSGGIFDYDAKSVRLIEVNKELEDPNVWNDSQHAQALGKEKKLLEGVVDVLSSLDNDLRDTKDLFDM
AREENDEDTLVACEEDVAKLTTRVEDMEFRRMFSNPADPNNAFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKTEVL
EESEGDVAGIKSATIKVEGEYAYGFLRTETGIHRLVRKSPFDSSGGRHTSFSSVFVYPEIDESFEIEVNPADLRIDTYRA
SGAGGQHINKTDSAVRITHIPSGIVVQCQNDRSQHRNRAEAMAMLKSRLYEAEMRKRQSEQDKLEAGKSDVGWGHQIRSY
VLDNSRIKDLRTNVEISNTKSVLDGDLDAFISASLKQGI

Sequences:

>Translated_359_residues
MTPLWRTCAHAQASSGGIFDYDAKSVRLIEVNKELEDPNVWNDSQHAQALGKEKKLLEGVVDVLSSLDNDLRDTKDLFDM
AREENDEDTLVACEEDVAKLTTRVEDMEFRRMFSNPADPNNAFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKTEVL
EESEGDVAGIKSATIKVEGEYAYGFLRTETGIHRLVRKSPFDSSGGRHTSFSSVFVYPEIDESFEIEVNPADLRIDTYRA
SGAGGQHINKTDSAVRITHIPSGIVVQCQNDRSQHRNRAEAMAMLKSRLYEAEMRKRQSEQDKLEAGKSDVGWGHQIRSY
VLDNSRIKDLRTNVEISNTKSVLDGDLDAFISASLKQGI
>Mature_358_residues
TPLWRTCAHAQASSGGIFDYDAKSVRLIEVNKELEDPNVWNDSQHAQALGKEKKLLEGVVDVLSSLDNDLRDTKDLFDMA
REENDEDTLVACEEDVAKLTTRVEDMEFRRMFSNPADPNNAFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKTEVLE
ESEGDVAGIKSATIKVEGEYAYGFLRTETGIHRLVRKSPFDSSGGRHTSFSSVFVYPEIDESFEIEVNPADLRIDTYRAS
GAGGQHINKTDSAVRITHIPSGIVVQCQNDRSQHRNRAEAMAMLKSRLYEAEMRKRQSEQDKLEAGKSDVGWGHQIRSYV
LDNSRIKDLRTNVEISNTKSVLDGDLDAFISASLKQGI

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=312, Percent_Identity=34.6153846153846, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI34577120, Length=250, Percent_Identity=36.8, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI166795305, Length=211, Percent_Identity=34.5971563981043, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=63.265306122449, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1787462, Length=246, Percent_Identity=42.6829268292683, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=286, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=311, Percent_Identity=33.4405144694534, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19921226, Length=290, Percent_Identity=35.5172413793103, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40256; Mature: 40125

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLWRTCAHAQASSGGIFDYDAKSVRLIEVNKELEDPNVWNDSQHAQALGKEKKLLEGV
CCCHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VDVLSSLDNDLRDTKDLFDMAREENDEDTLVACEEDVAKLTTRVEDMEFRRMFSNPADPN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCC
NAFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKTEVLEESEGDVAGIKSATIKVEGE
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCEEEEEEECC
YAYGFLRTETGIHRLVRKSPFDSSGGRHTSFSSVFVYPEIDESFEIEVNPADLRIDTYRA
EEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEC
SGAGGQHINKTDSAVRITHIPSGIVVQCQNDRSQHRNRAEAMAMLKSRLYEAEMRKRQSE
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QDKLEAGKSDVGWGHQIRSYVLDNSRIKDLRTNVEISNTKSVLDGDLDAFISASLKQGI
HHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHCCCEECCCHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TPLWRTCAHAQASSGGIFDYDAKSVRLIEVNKELEDPNVWNDSQHAQALGKEKKLLEGV
CCHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VDVLSSLDNDLRDTKDLFDMAREENDEDTLVACEEDVAKLTTRVEDMEFRRMFSNPADPN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCC
NAFIDIQAGAGGTEACDWASMLLRQYLRYCERKGFKTEVLEESEGDVAGIKSATIKVEGE
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCEEEEEEECC
YAYGFLRTETGIHRLVRKSPFDSSGGRHTSFSSVFVYPEIDESFEIEVNPADLRIDTYRA
EEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCEEEEEEEC
SGAGGQHINKTDSAVRITHIPSGIVVQCQNDRSQHRNRAEAMAMLKSRLYEAEMRKRQSE
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QDKLEAGKSDVGWGHQIRSYVLDNSRIKDLRTNVEISNTKSVLDGDLDAFISASLKQGI
HHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHCCCEECCCHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA