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The map label for this gene is fabI [H]

Identifier: 186476142

GI number: 186476142

Start: 1555179

End: 1555970

Strand: Direct

Name: fabI [H]

Synonym: Bphy_1384

Alternate gene names: 186476142

Gene position: 1555179-1555970 (Clockwise)

Preceding gene: 186476136

Following gene: 186476149

Centisome position: 44.7

GC content: 59.47

Gene sequence:

>792_bases
ATGGGCTTCCTCGCTGGTAAAAGAATCTTGCTGACTGGCCTCCTGTCGAACCGTTCGATCGCTTACGGTATTGCGCAGGC
CTGCAAGCGCGAAGGCGCAGAACTGGCGTTCACGTATGTCGGCGACCGCTTCAGGGACCGCATCACCGAATTCGCGAACG
AATTTGGCAGCAACCTGGTGTTCCCGTGCGACGTTGCCGACGATGCGCAAATCGAAGCGCTCTTCACTTCGCTGAAACAG
CATTGGGACAGTCTTGACGGTCTCGTCCATTCGATCGGCTTCGCGCCGCGCGAAGCGATCGCAGGCGACTTCCTCGACGG
CATGACGCGTGAAAATTTCCGCATCGCACACGATATCTCTGCGTATAGCTTCCCTGCCCTCGCGAAAGCCGCGCTGCCGA
TGCTGTCGAACGACGCGGCACTACTCACGCTGTCGTATCTCGGCGCAGAGAAAGCAATCCCCAACTACAACACGATGGGT
CTCGCGAAGGCTTCGCTCGAAGCGAGCGTGCGCTATCTGGCTGTGTCGCTGGGCAACAAGGGTATTCGCGTGAACGGCAT
TTCCGCCGGCCCCATCAAGACGCTCGCGGCAAGCGGCATCAAGGGCTTCGGCAAGATTCTCGATTTCGTCGAAGACAATG
CTCCGCTCAAGCGGAACGTGACGATTGAACAGGTCGGCAACGCCGCGGCGTTCCTGATGTCGGATCTGGCTGCTGGCGTG
ACGGCCGAGATCATGCACGTCGACAGCGGCTTCAGCAATGTGGTTGGCGGGATGGCAGCCGTCAGCGAGTAA

Upstream 100 bases:

>100_bases
GGTGGTCGGTGGGCGGTTGCAATGTGAGAGAATTCTACCCAAAATCCCGCGCGCTCCCGCACTGTGTCCGCGCGGCGCGC
TGACTTCATTAGGAGAATTC

Downstream 100 bases:

>100_bases
TCGCGGTTACCCATTCGCGTAAGCCGCCCGTGTCGTTGTGCGCGCAACAAAAACCGCCCCGGGCGGTTTTTTGCTTTGCG
CATGCGCTGCGATCAATGCC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MGFLAGKRILLTGLLSNRSIAYGIAQACKREGAELAFTYVGDRFRDRITEFANEFGSNLVFPCDVADDAQIEALFTSLKQ
HWDSLDGLVHSIGFAPREAIAGDFLDGMTRENFRIAHDISAYSFPALAKAALPMLSNDAALLTLSYLGAEKAIPNYNTMG
LAKASLEASVRYLAVSLGNKGIRVNGISAGPIKTLAASGIKGFGKILDFVEDNAPLKRNVTIEQVGNAAAFLMSDLAAGV
TAEIMHVDSGFSNVVGGMAAVSE

Sequences:

>Translated_263_residues
MGFLAGKRILLTGLLSNRSIAYGIAQACKREGAELAFTYVGDRFRDRITEFANEFGSNLVFPCDVADDAQIEALFTSLKQ
HWDSLDGLVHSIGFAPREAIAGDFLDGMTRENFRIAHDISAYSFPALAKAALPMLSNDAALLTLSYLGAEKAIPNYNTMG
LAKASLEASVRYLAVSLGNKGIRVNGISAGPIKTLAASGIKGFGKILDFVEDNAPLKRNVTIEQVGNAAAFLMSDLAAGV
TAEIMHVDSGFSNVVGGMAAVSE
>Mature_262_residues
GFLAGKRILLTGLLSNRSIAYGIAQACKREGAELAFTYVGDRFRDRITEFANEFGSNLVFPCDVADDAQIEALFTSLKQH
WDSLDGLVHSIGFAPREAIAGDFLDGMTRENFRIAHDISAYSFPALAKAALPMLSNDAALLTLSYLGAEKAIPNYNTMGL
AKASLEASVRYLAVSLGNKGIRVNGISAGPIKTLAASGIKGFGKILDFVEDNAPLKRNVTIEQVGNAAAFLMSDLAAGVT
AEIMHVDSGFSNVVGGMAAVSE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=63.0952380952381, Blast_Score=330, Evalue=5e-92,
Organism=Escherichia coli, GI1789378, Length=253, Percent_Identity=28.4584980237154, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082100, Length=272, Percent_Identity=28.3088235294118, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=25.4032258064516, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=26.4, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27872; Mature: 27741

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKRILLTGLLSNRSIAYGIAQACKREGAELAFTYVGDRFRDRITEFANEFGSNLV
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCE
FPCDVADDAQIEALFTSLKQHWDSLDGLVHSIGFAPREAIAGDFLDGMTRENFRIAHDIS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCC
AYSFPALAKAALPMLSNDAALLTLSYLGAEKAIPNYNTMGLAKASLEASVRYLAVSLGNK
HHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCC
GIRVNGISAGPIKTLAASGIKGFGKILDFVEDNAPLKRNVTIEQVGNAAAFLMSDLAAGV
CEEEECCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHCCC
TAEIMHVDSGFSNVVGGMAAVSE
EEEEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
GFLAGKRILLTGLLSNRSIAYGIAQACKREGAELAFTYVGDRFRDRITEFANEFGSNLV
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCE
FPCDVADDAQIEALFTSLKQHWDSLDGLVHSIGFAPREAIAGDFLDGMTRENFRIAHDIS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCC
AYSFPALAKAALPMLSNDAALLTLSYLGAEKAIPNYNTMGLAKASLEASVRYLAVSLGNK
HHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCC
GIRVNGISAGPIKTLAASGIKGFGKILDFVEDNAPLKRNVTIEQVGNAAAFLMSDLAAGV
CEEEECCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHCCC
TAEIMHVDSGFSNVVGGMAAVSE
EEEEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]