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The map label for this gene is gltX

Identifier: 186476135

GI number: 186476135

Start: 1545637

End: 1547046

Strand: Reverse

Name: gltX

Synonym: Bphy_1377

Alternate gene names: 186476135

Gene position: 1547046-1545637 (Counterclockwise)

Preceding gene: 186476137

Following gene: 186476134

Centisome position: 44.47

GC content: 63.05

Gene sequence:

>1410_bases
ATGACCACTCCCGTTCGCACCCGCTTCGCACCGAGCCCCACCGGCTTCATTCACCTCGGCAACATTCGCTCTGCGCTGTA
TCCGTGGGCGTTTGCGCGCAAATCGAAAGGCGTGTTCGTGCTGCGTATCGAGGACACGGACGTCGAGCGGTCGACCGAGC
AGTCCGTCGATGCGATTCTCGAAGGCATGCAATGGCTCGGCCTCGATTACGACGAAGGCCCGTTCTACCAGATGCAGCGC
ATGGACCGTTATCGCGAAGTGCTCAAGCACATGCAGGAGCAGGGGCTCGTGTACCCGTGCTACATGTCGACGGAAGAACT
CGACGCACTGCGCGAGCGCCAGCGCGCCTCTGGCGAGAAGCCGCGTTACGACGGCACGTGGCGTCCCGAGCCGGGCAAGG
TGCTGCCGACGCCGCCAGCGGGCGTCCAGCCAGTGTTGCGTTTCCGTAACCCGTTGACGGGCGTCGTCGCGTGGGACGAT
GCCGTGAAGGGCCGCGTCGAAATCTCGAACGAAGAACTCGACGATCTTGTGATCGCACGTCCGGACGGCACACCGACGTA
CAACTTCTGCGTGGTCGTCGACGACCTCGACATGAAGATCACGCACGTGATTCGCGGCGACGACCACGTGAACAATACGC
CGCGCCAGATCAACATCCTGCGTGCGCTCGGCGGCGAACCGCCCGTCTACGCACACCTGCCGACAGTGCTGAACGAGCAG
GGCGAGAAGATGAGCAAGCGTCATGGCGCGATGAGTGTGATGGGCTATCGCGACGCAGGCTATCTGCCCGAGGCCGTGCT
GAACTATCTGGCGCGACTGGGCTGGTCGCATGGCGACGCGGAAATCTTCTCGCGCGAGCAGCTGATCGAGTGGTTCGATC
TCGAACATCTGGGCAAGTCGCCCGCGCAGTACGACCACAACAAGCTCAACTGGCTGAACAACCACTACATCAAGGAAGCG
GACAATGCGCGTCTCGCAGATCTGAGCAAGCCGTTCTTCGCGGCGCTCGGCATCGACGAGCCGACGCTCGCGAAGGGTGC
GGATCTCGTTAGCGTGATTGCGCTAATGAAGGATCGCGCGTCGACGGTGAAGGAGGTCGTCGACAACGCAGCCATGTTCT
ATCGCGAGCCGAATCCGGATGCGGATGCGCTCGCACAACATGTGACGGACGCGGTGCGCCCGGCGCTCGCCGATCTCGCC
GCTGCATTGAAGGCGTGCGAGTGGACGAAGGAGGGCATCGCCGCCGCATTGAAGGCAACGCTTGGCGCGCACAAGCTGAA
GATGCCGCAGCTCGCGATGCCCGTGCGTGTGCTGGTGGCGGGCACGACGCACACGCCGTCCATCGACAGCGTGCTGATGC
TGTTCGGTCGCGACGTGGTGGTAAGCCGCATCGAAAAGGCGGTGGTCTGA

Upstream 100 bases:

>100_bases
CGCCGGCTGCTGTTGCCGTCGCCTGAAGCGAACCGTTTTCGTTGCCGCGCCCGGCATCGCTGTCCGCCGGCGCGGGCCGT
CCGTCTTCGAGTTACCGCAC

Downstream 100 bases:

>100_bases
TTCGCGTTTCACGCGACAGCGAGCAGGCGAAGCGAGGCCGCGGAGGACGATTGCGCGGCCTCGCAAAAAAATTGTAGAAA
GCTGCTCAAGAGGTATTTAC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTPVRTRFAPSPTGFIHLGNIRSALYPWAFARKSKGVFVLRIEDTDVERSTEQSVDAILEGMQWLGLDYDEGPFYQMQR
MDRYREVLKHMQEQGLVYPCYMSTEELDALRERQRASGEKPRYDGTWRPEPGKVLPTPPAGVQPVLRFRNPLTGVVAWDD
AVKGRVEISNEELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQLIEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEA
DNARLADLSKPFFAALGIDEPTLAKGADLVSVIALMKDRASTVKEVVDNAAMFYREPNPDADALAQHVTDAVRPALADLA
AALKACEWTKEGIAAALKATLGAHKLKMPQLAMPVRVLVAGTTHTPSIDSVLMLFGRDVVVSRIEKAVV

Sequences:

>Translated_469_residues
MTTPVRTRFAPSPTGFIHLGNIRSALYPWAFARKSKGVFVLRIEDTDVERSTEQSVDAILEGMQWLGLDYDEGPFYQMQR
MDRYREVLKHMQEQGLVYPCYMSTEELDALRERQRASGEKPRYDGTWRPEPGKVLPTPPAGVQPVLRFRNPLTGVVAWDD
AVKGRVEISNEELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQLIEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEA
DNARLADLSKPFFAALGIDEPTLAKGADLVSVIALMKDRASTVKEVVDNAAMFYREPNPDADALAQHVTDAVRPALADLA
AALKACEWTKEGIAAALKATLGAHKLKMPQLAMPVRVLVAGTTHTPSIDSVLMLFGRDVVVSRIEKAVV
>Mature_468_residues
TTPVRTRFAPSPTGFIHLGNIRSALYPWAFARKSKGVFVLRIEDTDVERSTEQSVDAILEGMQWLGLDYDEGPFYQMQRM
DRYREVLKHMQEQGLVYPCYMSTEELDALRERQRASGEKPRYDGTWRPEPGKVLPTPPAGVQPVLRFRNPLTGVVAWDDA
VKGRVEISNEELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQG
EKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQLIEWFDLEHLGKSPAQYDHNKLNWLNNHYIKEAD
NARLADLSKPFFAALGIDEPTLAKGADLVSVIALMKDRASTVKEVVDNAAMFYREPNPDADALAQHVTDAVRPALADLAA
ALKACEWTKEGIAAALKATLGAHKLKMPQLAMPVRVLVAGTTHTPSIDSVLMLFGRDVVVSRIEKAVV

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=506, Percent_Identity=29.6442687747036, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=48.9270386266094, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=34.3859649122807, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=28.8381742738589, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324540, Length=422, Percent_Identity=32.9383886255924, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24665321, Length=500, Percent_Identity=28, Blast_Score=207, Evalue=8e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_BURP8 (B2JIQ8)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857605.1
- GeneID:   6244651
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1377
- HOGENOM:   HBG628189
- OMA:   DEASWTK
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52377; Mature: 52246

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 246-246

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPVRTRFAPSPTGFIHLGNIRSALYPWAFARKSKGVFVLRIEDTDVERSTEQSVDAIL
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHH
EGMQWLGLDYDEGPFYQMQRMDRYREVLKHMQEQGLVYPCYMSTEELDALRERQRASGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPTPPAGVQPVLRFRNPLTGVVAWDDAVKGRVEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHH
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQLIEWFDLEHLGKS
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHNKLNWLNNHYIKEADNARLADLSKPFFAALGIDEPTLAKGADLVSVIALMKDRA
CCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCHHEEECCCCCCHHCCHHHHHHHHHHHHHH
STVKEVVDNAAMFYREPNPDADALAQHVTDAVRPALADLAAALKACEWTKEGIAAALKAT
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAHKLKMPQLAMPVRVLVAGTTHTPSIDSVLMLFGRDVVVSRIEKAVV
HHHHHCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPVRTRFAPSPTGFIHLGNIRSALYPWAFARKSKGVFVLRIEDTDVERSTEQSVDAIL
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHH
EGMQWLGLDYDEGPFYQMQRMDRYREVLKHMQEQGLVYPCYMSTEELDALRERQRASGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCC
PRYDGTWRPEPGKVLPTPPAGVQPVLRFRNPLTGVVAWDDAVKGRVEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEECHHHHHHH
GEKMSKRHGAMSVMGYRDAGYLPEAVLNYLARLGWSHGDAEIFSREQLIEWFDLEHLGKS
HHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYDHNKLNWLNNHYIKEADNARLADLSKPFFAALGIDEPTLAKGADLVSVIALMKDRA
CCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCHHEEECCCCCCHHCCHHHHHHHHHHHHHH
STVKEVVDNAAMFYREPNPDADALAQHVTDAVRPALADLAAALKACEWTKEGIAAALKAT
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAHKLKMPQLAMPVRVLVAGTTHTPSIDSVLMLFGRDVVVSRIEKAVV
HHHHHCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA