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The map label for this gene is ligA

Identifier: 186476101

GI number: 186476101

Start: 1504744

End: 1506795

Strand: Reverse

Name: ligA

Synonym: Bphy_1343

Alternate gene names: 186476101

Gene position: 1506795-1504744 (Counterclockwise)

Preceding gene: 186476103

Following gene: 186476100

Centisome position: 43.31

GC content: 64.04

Gene sequence:

>2052_bases
ATGGCCCGAACCCAAGTCCCTGATCCTGCAACCAGTGCACCTGCCGAGCGGGCGTTGTGGCTGCGCGACGAACTCGAGCG
CGCGAATTACGCGTACTACGTGCTCGACCAGCCGGACTTGCCCGACGCGGAATACGACAGGCTGTTCAAGGAACTGCAGC
AGATCGAAAGCGAGCATCCCGACCTGATCACGCCGGATTCGCCGACGCAGCGCGTCGGCGGCGAAGTGGCAAGCGGCTTT
CGGCCCGTCGTGCACGACATGCCGATGCTGTCGCTGAACAACGGCTTCTCGGACGAAGACATCGCTGCCTTCGACAAGCG
CGTGTCCGACACGCTGCACCACACGCCCGTCGACTACGCGTGCGAACTGAAATTCGACGGACTGGCCATTTCGCTGCGCT
ACGTGGATGGCCAGTTCACCCAGGCAGCGACGCGCGGTGACGGCGCGACGGGCGAAGACGTGACGGAGAACGTGCGCACC
ATTCGCTCGATTCCCCTGAAGCTCAAGGGCAAGCGCGTGCCGAAACTGGTCGACGTGCGCGGCGAAGTGCTGATGTTCAG
GCGCGATTTCGACAAGCTGAACCAGCGTCAGCGCGACGCGGGCCAGCGGGAGTTCGCCAACCCGCGCAATGCAGCAGCGG
GCAGCTTGCGCCAGCTCGATCCGAAGATGACGGCGCAGCGTCCGTTGTCGTTCTTTGCATACGGCATCGGTGTGCTCGAC
GGCATCGAGATGCCTGGCACGCACAGCGAACTGCTCGACTGGTATCACGAAATGGGCTTGCCCGTGAACAGCGAGCGCGC
CGTGGTGCAGGGCGCCGAAGGCGTGCTCGGTTTCTTCCATAAGGTTGGCGAGAAACGCGACAAGCTGCCGTACGACATCG
ACGGCGTCGTCTACAAGGTGAACCGCCGCGACGAGCAGGACGCGCTGGGTTTCGTGTCGCGCGCGCCACGTTTCGCGCTC
GCGCACAAGTTTCCCGCGCAGGAAGCACTGACAAAGCTGATCGCCATCGACGTGCAGGTCGGCAGAACGGGCGCGATTAC
GCCTGTCGCGCGGCTCGAGCCCGTGTTCGTCGGCGGTGCGACCGTGACGAATGCAACTCTGCATAACGAGGACGAAGTGC
GCCGCAAGGACATCCGCATCGGCGACACCGTCATCGTGCGGCGCGCGGGCGACGTGATTCCAGAGGTGGTCGGCGCGCTG
CTCGAACGGCGTCCCCCCGACGCCCGCGAGTTCGTGATGCCGACGCAATGTCCCGTATGCGGCTCCGCGATCGAACGGCT
GCCCGACGAGGCGATCGCGCGCTGTTCGGGCGGCCTGTTTTGCCCGGCGCAGCGCAAGCAGGCGCTGTGGCATTTCGCGC
AGCGTCGCGCACTGGACATTGACGGACTCGGCGAAAAAATCATCGATCAGCTTGTTGAACTCAATCTGGTGCGCACGCCC
GCCGATCTCTTCAATCTGGGCTTCGCGACACTCGCCGAACTCGACCGCTTCGCCGAGAAGTCCGCGCAGAACCTCATCGA
CTCGCTGGAGAAGGCCAAGCACACAACGCTGGCGCGCTTCATCTATGCGCTGGGTATCCGGCATGTCGGCGAATCGACGG
CGAAAGATCTGGCCAGGCACTTCGGCTCGCTCGATCCCATCATGAGCGCGACCGTCGAAGAACTGCTCGAAGTCAACGAC
GTCGGCCCTATCGTCGCGGAGGCGATCCATCAGTTTTTCGCCGAAGAGCACAACCGCACCGTGATCGAACAGCTGCGCGC
GCCTGGCAAAGTCACCTGGGCGGAAGGTCCACCCGCGCCGAAAGCGCCGCAGGGTGTGTTGGCGGGCAAGACGGTCGTGC
TGACAGGCACGCTGCCCAACATGGGCCGTGACGAGGCGAAAGAGTTGCTGGAGGCGGCCGGTGCGAAGGTGGCCGGTTCG
GTGTCGAAGAAGACCGATTACGTGGTGGCCGGTGCGGAAGCGGGGAGCAAGCTCGCGAAAGCCGAGGAACTTGGCATCCC
CGTGCTCGACGAAGATGGTATGCGTAAGCTCCTGGAGGGGCAAACAACATGA

Upstream 100 bases:

>100_bases
GGGTTTGGACGCGCACGAAACTGGCCATCCGGCCGATGCGACAGAAAGCGCTTCCTGAGATAATCTGACATCCCGTTACT
CTTGCCGAAACGCCGACAGC

Downstream 100 bases:

>100_bases
TTCACGAAATCCTCAAGATGGGCGACCCGCGCCTGTTGCGCATCGCCAAGCCGGTCGATCATTTCGACACGCCCGAACTG
CACGAACTCGTGCGCGACAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MARTQVPDPATSAPAERALWLRDELERANYAYYVLDQPDLPDAEYDRLFKELQQIESEHPDLITPDSPTQRVGGEVASGF
RPVVHDMPMLSLNNGFSDEDIAAFDKRVSDTLHHTPVDYACELKFDGLAISLRYVDGQFTQAATRGDGATGEDVTENVRT
IRSIPLKLKGKRVPKLVDVRGEVLMFRRDFDKLNQRQRDAGQREFANPRNAAAGSLRQLDPKMTAQRPLSFFAYGIGVLD
GIEMPGTHSELLDWYHEMGLPVNSERAVVQGAEGVLGFFHKVGEKRDKLPYDIDGVVYKVNRRDEQDALGFVSRAPRFAL
AHKFPAQEALTKLIAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGAL
LERRPPDAREFVMPTQCPVCGSAIERLPDEAIARCSGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVELNLVRTP
ADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLARHFGSLDPIMSATVEELLEVND
VGPIVAEAIHQFFAEEHNRTVIEQLRAPGKVTWAEGPPAPKAPQGVLAGKTVVLTGTLPNMGRDEAKELLEAAGAKVAGS
VSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGMRKLLEGQTT

Sequences:

>Translated_683_residues
MARTQVPDPATSAPAERALWLRDELERANYAYYVLDQPDLPDAEYDRLFKELQQIESEHPDLITPDSPTQRVGGEVASGF
RPVVHDMPMLSLNNGFSDEDIAAFDKRVSDTLHHTPVDYACELKFDGLAISLRYVDGQFTQAATRGDGATGEDVTENVRT
IRSIPLKLKGKRVPKLVDVRGEVLMFRRDFDKLNQRQRDAGQREFANPRNAAAGSLRQLDPKMTAQRPLSFFAYGIGVLD
GIEMPGTHSELLDWYHEMGLPVNSERAVVQGAEGVLGFFHKVGEKRDKLPYDIDGVVYKVNRRDEQDALGFVSRAPRFAL
AHKFPAQEALTKLIAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGAL
LERRPPDAREFVMPTQCPVCGSAIERLPDEAIARCSGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVELNLVRTP
ADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLARHFGSLDPIMSATVEELLEVND
VGPIVAEAIHQFFAEEHNRTVIEQLRAPGKVTWAEGPPAPKAPQGVLAGKTVVLTGTLPNMGRDEAKELLEAAGAKVAGS
VSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGMRKLLEGQTT
>Mature_682_residues
ARTQVPDPATSAPAERALWLRDELERANYAYYVLDQPDLPDAEYDRLFKELQQIESEHPDLITPDSPTQRVGGEVASGFR
PVVHDMPMLSLNNGFSDEDIAAFDKRVSDTLHHTPVDYACELKFDGLAISLRYVDGQFTQAATRGDGATGEDVTENVRTI
RSIPLKLKGKRVPKLVDVRGEVLMFRRDFDKLNQRQRDAGQREFANPRNAAAGSLRQLDPKMTAQRPLSFFAYGIGVLDG
IEMPGTHSELLDWYHEMGLPVNSERAVVQGAEGVLGFFHKVGEKRDKLPYDIDGVVYKVNRRDEQDALGFVSRAPRFALA
HKFPAQEALTKLIAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGALL
ERRPPDAREFVMPTQCPVCGSAIERLPDEAIARCSGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVELNLVRTPA
DLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLARHFGSLDPIMSATVEELLEVNDV
GPIVAEAIHQFFAEEHNRTVIEQLRAPGKVTWAEGPPAPKAPQGVLAGKTVVLTGTLPNMGRDEAKELLEAAGAKVAGSV
SKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGMRKLLEGQTT

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=47.2527472527472, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788750, Length=664, Percent_Identity=58.1325301204819, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=526, Percent_Identity=23.9543726235741, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62472391, Length=80, Percent_Identity=48.75, Blast_Score=69, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737765, Length=80, Percent_Identity=48.75, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BURP8 (B2JID1)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857571.1
- ProteinModelPortal:   B2JID1
- GeneID:   6242834
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_1343
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK930848
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75014; Mature: 74883

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 124-124 BINDING 122-122 BINDING 145-145 BINDING 182-182 BINDING 299-299 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTQVPDPATSAPAERALWLRDELERANYAYYVLDQPDLPDAEYDRLFKELQQIESEHP
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
DLITPDSPTQRVGGEVASGFRPVVHDMPMLSLNNGFSDEDIAAFDKRVSDTLHHTPVDYA
CCCCCCCCHHHHCHHHHHCCHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCEE
CELKFDGLAISLRYVDGQFTQAATRGDGATGEDVTENVRTIRSIPLKLKGKRVPKLVDVR
EEEEECCEEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHCC
GEVLMFRRDFDKLNQRQRDAGQREFANPRNAAAGSLRQLDPKMTAQRPLSFFAYGIGVLD
CHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHCHHHHCCCHHHHHCCHHHHHHHHHHHC
GIEMPGTHSELLDWYHEMGLPVNSERAVVQGAEGVLGFFHKVGEKRDKLPYDIDGVVYKV
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
NRRDEQDALGFVSRAPRFALAHKFPAQEALTKLIAIDVQVGRTGAITPVARLEPVFVGGA
CCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHCCEEEECCC
TVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGALLERRPPDAREFVMPTQCPVC
EEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHH
GSAIERLPDEAIARCSGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVELNLVRTP
HHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
ADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLARH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FGSLDPIMSATVEELLEVNDVGPIVAEAIHQFFAEEHNRTVIEQLRAPGKVTWAEGPPAP
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCCC
KAPQGVLAGKTVVLTGTLPNMGRDEAKELLEAAGAKVAGSVSKKTDYVVAGAEAGSKLAK
CCCCCEECCCEEEEEECCCCCCHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCHHHHH
AEELGIPVLDEDGMRKLLEGQTT
HHHCCCCCCCCHHHHHHHCCCCC
>Mature Secondary Structure 
ARTQVPDPATSAPAERALWLRDELERANYAYYVLDQPDLPDAEYDRLFKELQQIESEHP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
DLITPDSPTQRVGGEVASGFRPVVHDMPMLSLNNGFSDEDIAAFDKRVSDTLHHTPVDYA
CCCCCCCCHHHHCHHHHHCCHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCEE
CELKFDGLAISLRYVDGQFTQAATRGDGATGEDVTENVRTIRSIPLKLKGKRVPKLVDVR
EEEEECCEEEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCCCHHHHCC
GEVLMFRRDFDKLNQRQRDAGQREFANPRNAAAGSLRQLDPKMTAQRPLSFFAYGIGVLD
CHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHCHHHHCCCHHHHHCCHHHHHHHHHHHC
GIEMPGTHSELLDWYHEMGLPVNSERAVVQGAEGVLGFFHKVGEKRDKLPYDIDGVVYKV
CCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
NRRDEQDALGFVSRAPRFALAHKFPAQEALTKLIAIDVQVGRTGAITPVARLEPVFVGGA
CCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHCCEEEECCC
TVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGALLERRPPDAREFVMPTQCPVC
EEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHH
GSAIERLPDEAIARCSGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVELNLVRTP
HHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
ADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLARH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FGSLDPIMSATVEELLEVNDVGPIVAEAIHQFFAEEHNRTVIEQLRAPGKVTWAEGPPAP
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEECCCCCCC
KAPQGVLAGKTVVLTGTLPNMGRDEAKELLEAAGAKVAGSVSKKTDYVVAGAEAGSKLAK
CCCCCEECCCEEEEEECCCCCCHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCHHHHH
AEELGIPVLDEDGMRKLLEGQTT
HHHCCCCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA