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The map label for this gene is def1 [H]

Identifier: 186476100

GI number: 186476100

Start: 1504214

End: 1504747

Strand: Reverse

Name: def1 [H]

Synonym: Bphy_1342

Alternate gene names: 186476100

Gene position: 1504747-1504214 (Counterclockwise)

Preceding gene: 186476101

Following gene: 186476096

Centisome position: 43.25

GC content: 60.67

Gene sequence:

>534_bases
ATGATTCACGAAATCCTCAAGATGGGCGACCCGCGCCTGTTGCGCATCGCCAAGCCGGTCGATCATTTCGACACGCCCGA
ACTGCACGAACTCGTGCGCGACATGTTCGAAACGATGCATGACGCGAACGGCGCCGGGCTCGCGGCGCCGCAGATCGGTG
TTGACCTGCAGGTCGTGATTTTCGGTTTCGGCCACAACGAGCGTTATCCCGACGCGCCGTCCGTGCCCGAAACGGTGCTG
ATCAATCCGACCATCACGCCCGTTTCGCTCGACACGGAAGAGGGTTGGGAAGGGTGCCTGTCGGTGCCCGGTCTGCGGGG
GGCGGTGAGCCGCCTGTCGATGATCCGCTATCACGGCTTCGATCAGTTCGGCAAACCGATCGATCGCGTCGCCGAGGGTT
TCCATGCGCGCGTCGTTCAGCATGAGTGCGATCACCTGATCGGCAAGCTCTATCCGATGCGAATCACGGATTTCGCGAAA
TTCGGCTTTACCGAGGTGCTTTTTCCCGACCTCGATCCGAAAGCGGACGATTGA

Upstream 100 bases:

>100_bases
CCGGTGCGGAAGCGGGGAGCAAGCTCGCGAAAGCCGAGGAACTTGGCATCCCCGTGCTCGACGAAGATGGTATGCGTAAG
CTCCTGGAGGGGCAAACAAC

Downstream 100 bases:

>100_bases
TCTACGAACTTCGATATAAGAAAAGCCCGCGGATGCGGGCTTTTTGTTTGCATAAGACAGCGCTTGTTCGCGCTTAGAAC
GACTCGTCCGGACCGAGGTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIHEILKMGDPRLLRIAKPVDHFDTPELHELVRDMFETMHDANGAGLAAPQIGVDLQVVIFGFGHNERYPDAPSVPETVL
INPTITPVSLDTEEGWEGCLSVPGLRGAVSRLSMIRYHGFDQFGKPIDRVAEGFHARVVQHECDHLIGKLYPMRITDFAK
FGFTEVLFPDLDPKADD

Sequences:

>Translated_177_residues
MIHEILKMGDPRLLRIAKPVDHFDTPELHELVRDMFETMHDANGAGLAAPQIGVDLQVVIFGFGHNERYPDAPSVPETVL
INPTITPVSLDTEEGWEGCLSVPGLRGAVSRLSMIRYHGFDQFGKPIDRVAEGFHARVVQHECDHLIGKLYPMRITDFAK
FGFTEVLFPDLDPKADD
>Mature_177_residues
MIHEILKMGDPRLLRIAKPVDHFDTPELHELVRDMFETMHDANGAGLAAPQIGVDLQVVIFGFGHNERYPDAPSVPETVL
INPTITPVSLDTEEGWEGCLSVPGLRGAVSRLSMIRYHGFDQFGKPIDRVAEGFHARVVQHECDHLIGKLYPMRITDFAK
FGFTEVLFPDLDPKADD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=30.8571428571429, Blast_Score=77, Evalue=9e-15,
Organism=Escherichia coli, GI1789682, Length=151, Percent_Identity=38.4105960264901, Blast_Score=98, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=35.2564102564103, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24645728, Length=167, Percent_Identity=30.5389221556886, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19802; Mature: 19802

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHEILKMGDPRLLRIAKPVDHFDTPELHELVRDMFETMHDANGAGLAAPQIGVDLQVVI
CHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
FGFGHNERYPDAPSVPETVLINPTITPVSLDTEEGWEGCLSVPGLRGAVSRLSMIRYHGF
EECCCCCCCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCC
DQFGKPIDRVAEGFHARVVQHECDHLIGKLYPMRITDFAKFGFTEVLFPDLDPKADD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MIHEILKMGDPRLLRIAKPVDHFDTPELHELVRDMFETMHDANGAGLAAPQIGVDLQVVI
CHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
FGFGHNERYPDAPSVPETVLINPTITPVSLDTEEGWEGCLSVPGLRGAVSRLSMIRYHGF
EECCCCCCCCCCCCCCCEEEECCCCCEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCC
DQFGKPIDRVAEGFHARVVQHECDHLIGKLYPMRITDFAKFGFTEVLFPDLDPKADD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]