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The map label for this gene is citH [H]

Identifier: 186475925

GI number: 186475925

Start: 1300542

End: 1301864

Strand: Direct

Name: citH [H]

Synonym: Bphy_1160

Alternate gene names: 186475925

Gene position: 1300542-1301864 (Clockwise)

Preceding gene: 186475924

Following gene: 186475926

Centisome position: 37.38

GC content: 65.31

Gene sequence:

>1323_bases
ATGAATATGGATTCCGTCACCGCGCATCATCCCTCGCACGCGTCGCACGCGTCACACGATGCCGGCCCGTCCGCCACGTC
GGGCAAGGGCGTCGCGCGCCTGATCGTCGCCACGTCGATCGGCAATGCGCTCGAGTTCTACGACCTGATCGTGTACGGCT
ACTTCGCGACCACGCTGTCGAAGCTGTTCTTTCCTGCACACAACGCGACCGTCTCGCTGCTGCTCACGCTCGGCACCTTC
GCGCTGTCGTATCTCGCGCGGCCCATCGGCGCGCTCGTGCTCGGCTCGTATAGCGACCGCAAGGGCCGCAAGGCATCGCT
GACGCTATCGATCGCCATGATGACGCTCGGGACGGGGATGGTCGCGCTGATGCCGACCTACGCGACGATCGGCCTGCTCG
CGCCTGTCGGCATTTTCGTGTCGCGCCTGTTGCAAGGTTTCTCGGCGGGCGGCGAATTCGGCAGCTCGACGGCGTTCCTG
ATCGAACACGCGCCCAGGCGCAGCGGCTTCATGTCGAGCTGGCAGTTCTCCAGTCAGGGCGCGAGCACGTTGCTCGCGTC
CGCGTTCGGCGCGGTGCTGACGGGTATGCTCACGCCGCCGCAGCTGGAAGGCTGGGGTTGGCGCATTCCGTTTCTGTTCG
GCATGCTGATCGGTCCCGTCGGGCTCTATATCCGCCGCCGCATCGACGAGACGCCCGAATTCGAGCGCGCCGAAAAAGCC
GAGTCGCCCGTGCGCGAACTGCTCGCGACGCAAAAGTCGCGCGTGCTGGTGTCGATCGGCGCGCTCGTGCTGACGACGAC
GGCCAATTACATGATCCTCTACATGCCGACTTACGCCTCGCGGCAACTCGGACTCGCACCGTCGTCGGGCTTCATCGCGA
CGCTGATCGCAGGGTTGATCGTCACGGTGCTCACGCCCATCGTCGGACACTGGTCGGACAAGGTGGGCCGCACACGCATC
ATGCTTGGCGCGGGTGCGCTCTTCTTCGTCACGGTGTATCCCGCGTTCATGTTCATGAACGCGCACCCGTCGTTGCCGAC
GCTGCTCGCCGCCGTCAGCTGGGTCGCGCTGCTGAAGGCCACGTACTTCGCACCGATTCCCGCGTTGATGGCCGAACTCT
TTCCGACCCGCACACGCACCACGGGCATGGCCTTCGGCTATAACATCGGCACGACGATATTCGGCGGTTTCACGCCGCTC
GCGGTCGCGTCGCTGATCGCCGCGACGGGCAATAACCTCGCGCCGGGTCTCTATCTGATGATGGCGGCCGTCATCAGCCT
GCTGACCCTGGTCTGGGCGCGGGCGCGGCTGAACGCACACTGA

Upstream 100 bases:

>100_bases
CGCATCATGGCCGGTCTCTTCTGGGACCTGGGCGCCCACGAAGCCGGTGCAACCAATCCCGCTCCGCATGTTTCGACCAC
CCCAGCCTGAGGACGTCGCA

Downstream 100 bases:

>100_bases
ATCGAATGAATCACGAGGGACGCGCACGTATGCAAGCCGATTTGCCGCAAGCACTGAAAGAAGCGATCCAGTTTTCGATC
GATCACGAATCGCCGTGGGA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MNMDSVTAHHPSHASHASHDAGPSATSGKGVARLIVATSIGNALEFYDLIVYGYFATTLSKLFFPAHNATVSLLLTLGTF
ALSYLARPIGALVLGSYSDRKGRKASLTLSIAMMTLGTGMVALMPTYATIGLLAPVGIFVSRLLQGFSAGGEFGSSTAFL
IEHAPRRSGFMSSWQFSSQGASTLLASAFGAVLTGMLTPPQLEGWGWRIPFLFGMLIGPVGLYIRRRIDETPEFERAEKA
ESPVRELLATQKSRVLVSIGALVLTTTANYMILYMPTYASRQLGLAPSSGFIATLIAGLIVTVLTPIVGHWSDKVGRTRI
MLGAGALFFVTVYPAFMFMNAHPSLPTLLAAVSWVALLKATYFAPIPALMAELFPTRTRTTGMAFGYNIGTTIFGGFTPL
AVASLIAATGNNLAPGLYLMMAAVISLLTLVWARARLNAH

Sequences:

>Translated_440_residues
MNMDSVTAHHPSHASHASHDAGPSATSGKGVARLIVATSIGNALEFYDLIVYGYFATTLSKLFFPAHNATVSLLLTLGTF
ALSYLARPIGALVLGSYSDRKGRKASLTLSIAMMTLGTGMVALMPTYATIGLLAPVGIFVSRLLQGFSAGGEFGSSTAFL
IEHAPRRSGFMSSWQFSSQGASTLLASAFGAVLTGMLTPPQLEGWGWRIPFLFGMLIGPVGLYIRRRIDETPEFERAEKA
ESPVRELLATQKSRVLVSIGALVLTTTANYMILYMPTYASRQLGLAPSSGFIATLIAGLIVTVLTPIVGHWSDKVGRTRI
MLGAGALFFVTVYPAFMFMNAHPSLPTLLAAVSWVALLKATYFAPIPALMAELFPTRTRTTGMAFGYNIGTTIFGGFTPL
AVASLIAATGNNLAPGLYLMMAAVISLLTLVWARARLNAH
>Mature_440_residues
MNMDSVTAHHPSHASHASHDAGPSATSGKGVARLIVATSIGNALEFYDLIVYGYFATTLSKLFFPAHNATVSLLLTLGTF
ALSYLARPIGALVLGSYSDRKGRKASLTLSIAMMTLGTGMVALMPTYATIGLLAPVGIFVSRLLQGFSAGGEFGSSTAFL
IEHAPRRSGFMSSWQFSSQGASTLLASAFGAVLTGMLTPPQLEGWGWRIPFLFGMLIGPVGLYIRRRIDETPEFERAEKA
ESPVRELLATQKSRVLVSIGALVLTTTANYMILYMPTYASRQLGLAPSSGFIATLIAGLIVTVLTPIVGHWSDKVGRTRI
MLGAGALFFVTVYPAFMFMNAHPSLPTLLAAVSWVALLKATYFAPIPALMAELFPTRTRTTGMAFGYNIGTTIFGGFTPL
AVASLIAATGNNLAPGLYLMMAAVISLLTLVWARARLNAH

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=33.0188679245283, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1788942, Length=403, Percent_Identity=33.9950372208437, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788292, Length=400, Percent_Identity=28.5, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1789941, Length=437, Percent_Identity=32.4942791762014, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI87082231, Length=168, Percent_Identity=29.7619047619048, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082404, Length=359, Percent_Identity=25.6267409470752, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46814; Mature: 46814

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMDSVTAHHPSHASHASHDAGPSATSGKGVARLIVATSIGNALEFYDLIVYGYFATTLS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KLFFPAHNATVSLLLTLGTFALSYLARPIGALVLGSYSDRKGRKASLTLSIAMMTLGTGM
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHCCCH
VALMPTYATIGLLAPVGIFVSRLLQGFSAGGEFGSSTAFLIEHAPRRSGFMSSWQFSSQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
ASTLLASAFGAVLTGMLTPPQLEGWGWRIPFLFGMLIGPVGLYIRRRIDETPEFERAEKA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ESPVRELLATQKSRVLVSIGALVLTTTANYMILYMPTYASRQLGLAPSSGFIATLIAGLI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
VTVLTPIVGHWSDKVGRTRIMLGAGALFFVTVYPAFMFMNAHPSLPTLLAAVSWVALLKA
HHHHHHHHHHCCHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TYFAPIPALMAELFPTRTRTTGMAFGYNIGTTIFGGFTPLAVASLIAATGNNLAPGLYLM
HHHCCHHHHHHHHCCCCCCCCCCEECCCCCHHHHCCHHHHHHHHHHHHCCCCCCHHHHHH
MAAVISLLTLVWARARLNAH
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNMDSVTAHHPSHASHASHDAGPSATSGKGVARLIVATSIGNALEFYDLIVYGYFATTLS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KLFFPAHNATVSLLLTLGTFALSYLARPIGALVLGSYSDRKGRKASLTLSIAMMTLGTGM
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHCCCH
VALMPTYATIGLLAPVGIFVSRLLQGFSAGGEFGSSTAFLIEHAPRRSGFMSSWQFSSQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
ASTLLASAFGAVLTGMLTPPQLEGWGWRIPFLFGMLIGPVGLYIRRRIDETPEFERAEKA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ESPVRELLATQKSRVLVSIGALVLTTTANYMILYMPTYASRQLGLAPSSGFIATLIAGLI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
VTVLTPIVGHWSDKVGRTRIMLGAGALFFVTVYPAFMFMNAHPSLPTLLAAVSWVALLKA
HHHHHHHHHHCCHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TYFAPIPALMAELFPTRTRTTGMAFGYNIGTTIFGGFTPLAVASLIAATGNNLAPGLYLM
HHHCCHHHHHHHHCCCCCCCCCCEECCCCCHHHHCCHHHHHHHHHHHHCCCCCCHHHHHH
MAAVISLLTLVWARARLNAH
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]