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The map label for this gene is glnH [H]

Identifier: 186475921

GI number: 186475921

Start: 1295373

End: 1296119

Strand: Reverse

Name: glnH [H]

Synonym: Bphy_1156

Alternate gene names: 186475921

Gene position: 1296119-1295373 (Counterclockwise)

Preceding gene: 186475922

Following gene: 186475920

Centisome position: 37.25

GC content: 61.18

Gene sequence:

>747_bases
ATGAAACTCGTAGCAAAGCTGGCTGCGGCCGCACTCGTCGCCGTATCTGTTCTCGGTCATGCGGCGCGTGCCGAGACCCT
GCTGGTTGCCGTCGACACGGCGTTCGTGCCGTTCGAATTCAAGCAGGGCGACAAGTACGTCGGCTTCGACATCGACCTGT
GGGAAGCCGTCGCGAAAGACGTGAAGATCGACTACAAGCTGCAGCCGATGGACTTCAGCGGCATCCTGCCCGCCTTGCAG
ACGCATAACGTCGACGTCGCGCTTGCGGGCATCACGATCAAGGACGAGCGCAAGAAAGTGATCGACTTCTCCGACGGCTA
CTACGACAGTGGCTTCATGCTGATGGTGCCGGTCACGAGCACGATCAAGGGACCGGACGACCTGAAGGGCAAGTCGCTTG
CGATCAAGACGGGCACGTCGGCGGCGGACTACGCAAAGGCGCATTTCGCGGGGACCGATCTGCGTCAGTTCCCGAACATC
GACAACGCGTATCTGGAGTTGCAGACGGGACGCGTCGATGCCGCAATGCACGACACGCCGAACGTGCTCTATTACATCCA
CACGGCGGGCCAGGGACGCGTAAAGGCAGTCGGGCCGCAAATGATGGCGCAGCAATACGGCATCGCGTTCCCGAAGGGCA
GTCCGCTCGTGGCGAAGGTCAATGCGGCGATTGCGAAGATCAAGGCTGACGGCCGTTACGCGGCGATCTACAAGAAGTGG
TTCGGCGTCGAGCCGGCGAAGTCCTGA

Upstream 100 bases:

>100_bases
AATTATTTTGCACAAAGATACTCAGCGCAAATCGGTTTGCAATCGAGCCGGTTCAACCGTTCATCCACTCATCATCCACT
CACCGCGCAAGGGGATCGTC

Downstream 100 bases:

>100_bases
ACAACATCGGCAGCGGGAGAGTAGCCATGCAGTTCGATCTGTCCGTCGTCGCCGATGCCTTGCCTGCGTTGCTGCAGGGC
GCAAAGCTGACGGTGCTGAT

Product: glutamine ABC transporter periplasmic protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKLVAKLAAAALVAVSVLGHAARAETLLVAVDTAFVPFEFKQGDKYVGFDIDLWEAVAKDVKIDYKLQPMDFSGILPALQ
THNVDVALAGITIKDERKKVIDFSDGYYDSGFMLMVPVTSTIKGPDDLKGKSLAIKTGTSAADYAKAHFAGTDLRQFPNI
DNAYLELQTGRVDAAMHDTPNVLYYIHTAGQGRVKAVGPQMMAQQYGIAFPKGSPLVAKVNAAIAKIKADGRYAAIYKKW
FGVEPAKS

Sequences:

>Translated_248_residues
MKLVAKLAAAALVAVSVLGHAARAETLLVAVDTAFVPFEFKQGDKYVGFDIDLWEAVAKDVKIDYKLQPMDFSGILPALQ
THNVDVALAGITIKDERKKVIDFSDGYYDSGFMLMVPVTSTIKGPDDLKGKSLAIKTGTSAADYAKAHFAGTDLRQFPNI
DNAYLELQTGRVDAAMHDTPNVLYYIHTAGQGRVKAVGPQMMAQQYGIAFPKGSPLVAKVNAAIAKIKADGRYAAIYKKW
FGVEPAKS
>Mature_248_residues
MKLVAKLAAAALVAVSVLGHAARAETLLVAVDTAFVPFEFKQGDKYVGFDIDLWEAVAKDVKIDYKLQPMDFSGILPALQ
THNVDVALAGITIKDERKKVIDFSDGYYDSGFMLMVPVTSTIKGPDDLKGKSLAIKTGTSAADYAKAHFAGTDLRQFPNI
DNAYLELQTGRVDAAMHDTPNVLYYIHTAGQGRVKAVGPQMMAQQYGIAFPKGSPLVAKVNAAIAKIKADGRYAAIYKKW
FGVEPAKS

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=64.1129032258064, Blast_Score=320, Evalue=4e-89,
Organism=Escherichia coli, GI1788228, Length=250, Percent_Identity=32.8, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1787085, Length=241, Percent_Identity=32.3651452282158, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=32.0987654320988, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788649, Length=250, Percent_Identity=30.8, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=27.9475982532751, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26788; Mature: 26788

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVAKLAAAALVAVSVLGHAARAETLLVAVDTAFVPFEFKQGDKYVGFDIDLWEAVAKD
CHHHHHHHHHHHHHHHHHHCHHCCCEEEEEEECEEEEEEECCCCEEEECCHHHHHHHHHC
VKIDYKLQPMDFSGILPALQTHNVDVALAGITIKDERKKVIDFSDGYYDSGFMLMVPVTS
EEEEEEECCCCCCCCCCHHHCCCCEEEEEEEEEECCCCEEEECCCCEECCCCEEEEEECC
TIKGPDDLKGKSLAIKTGTSAADYAKAHFAGTDLRQFPNIDNAYLELQTGRVDAAMHDTP
CCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCEEEEECCCC
NVLYYIHTAGQGRVKAVGPQMMAQQYGIAFPKGSPLVAKVNAAIAKIKADGRYAAIYKKW
CEEEEEEECCCCEEEEECHHHHHHHCCEECCCCCCEEEEECEEEEEEECCCCEEEEEHHH
FGVEPAKS
CCCCCCCC
>Mature Secondary Structure
MKLVAKLAAAALVAVSVLGHAARAETLLVAVDTAFVPFEFKQGDKYVGFDIDLWEAVAKD
CHHHHHHHHHHHHHHHHHHCHHCCCEEEEEEECEEEEEEECCCCEEEECCHHHHHHHHHC
VKIDYKLQPMDFSGILPALQTHNVDVALAGITIKDERKKVIDFSDGYYDSGFMLMVPVTS
EEEEEEECCCCCCCCCCHHHCCCCEEEEEEEEEECCCCEEEECCCCEECCCCEEEEEECC
TIKGPDDLKGKSLAIKTGTSAADYAKAHFAGTDLRQFPNIDNAYLELQTGRVDAAMHDTP
CCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCEEEEECCCC
NVLYYIHTAGQGRVKAVGPQMMAQQYGIAFPKGSPLVAKVNAAIAKIKADGRYAAIYKKW
CEEEEEEECCCCEEEEECHHHHHHHCCEECCCCCCEEEEECEEEEEEECCCCEEEEEHHH
FGVEPAKS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]