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The map label for this gene is gca [H]

Identifier: 186475834

GI number: 186475834

Start: 1201886

End: 1202929

Strand: Reverse

Name: gca [H]

Synonym: Bphy_1069

Alternate gene names: 186475834

Gene position: 1202929-1201886 (Counterclockwise)

Preceding gene: 186475835

Following gene: 186475821

Centisome position: 34.58

GC content: 62.26

Gene sequence:

>1044_bases
ATGACCACACAACGCAAAGCCATCATCACAGGCGTATCCGGGCAGGACGGCGCCTATCTGACGAAGCTGCTGCTCAGCAA
GGGCTATCAGGTCACGGGCACGTATCGCCGCACCAGTTCCGTCAATTTCTGGCGCATGGAAGAACTGGGCGTGATGGACA
ACCCGCATCTGCAACTCGTCGAACACGATCTGACGGACGCCGGTTCGACGCTGCGCCTGCTCGATACGGTGCAGGCTGAC
GAACTCTACAATCTCGCCGCGCAAAGCTTCGTCGGCGTGTCGTTCGACCAGCCTTCGACGACGGCGCAAGTGACGGGCGT
GGGCGTGCTCAACCTGCTCGAAGGCATCCGCACGGTGAATCCGCAGATGCGTTTCTATCAGGCATCCACGTCGGAGATGT
TCGGCAAGGTGCAGGCCGTGCCGCAAGCCGAGGACACGCCGTTCTATCCGCGCAGCCCCTATGGTGTTGCGAAGCTGTTC
GCGCACTGGTCGACGATCAACTATCGCGAGTCGTACGGCATGTTCGCGACAAGCGGCATTCTGTTCAATCACGAGTCGCC
GCTGCGCGGCCGCGAGTTCGTCACGCGCAAGATCACCGACACCGTCGCGAAGATCAAGCTCGGCAAGGCCGACGTGCTCG
AACTCGGCAATCTCGATGCCAAGCGCGACTGGGGCTTCGCGCTCGAGTACGTGGAAGGCATGTGGCGCATGCTGCAAGCG
GATGAACCGGACACCTACGTGCTCGCGACGAACCGTACGGAGACGGTGCGCGACTTCGTGAAGATGGCGTTTGCAGCGGC
GGGCTATCAGCTCGAATGGACGGGCCGCGACGAGCGCGAGACGGGCATCGACATCTCGACGGGCAAGACGCTGATTCGCG
TGAATCCGCGCTTCTACCGCCCCGCCGAAGTCGACCTGCTGATCGGCTGCGCGGACAAAGCGCGTGAAAAGCTCGGCTGG
CGTCCGCAGACGTCGCTCGAACAACTGTGCCGGATGATGGTGGAGGCGGATATCGCGCGGAACCAGAACGGCGTGTCATG
CTGA

Upstream 100 bases:

>100_bases
TGCGGCGCCTGCTCGGCGTGCCCGCGCTGTCGGAAGGCGAGACGCCGCGTTCCCCTTCCCCTCCTCATTCAACTTCAGAC
AACCCGACGAGAGGCAACTC

Downstream 100 bases:

>100_bases
CATCCGTCGACGAGCAAGGGGCACGTAAAGCGCCCTTTGTTTTACCCGATTGACCCGATTGCTAGAGTAGCTTGCGGTCG
ACCTGCGGCACCATCGGCGC

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTTQRKAIITGVSGQDGAYLTKLLLSKGYQVTGTYRRTSSVNFWRMEELGVMDNPHLQLVEHDLTDAGSTLRLLDTVQAD
ELYNLAAQSFVGVSFDQPSTTAQVTGVGVLNLLEGIRTVNPQMRFYQASTSEMFGKVQAVPQAEDTPFYPRSPYGVAKLF
AHWSTINYRESYGMFATSGILFNHESPLRGREFVTRKITDTVAKIKLGKADVLELGNLDAKRDWGFALEYVEGMWRMLQA
DEPDTYVLATNRTETVRDFVKMAFAAAGYQLEWTGRDERETGIDISTGKTLIRVNPRFYRPAEVDLLIGCADKAREKLGW
RPQTSLEQLCRMMVEADIARNQNGVSC

Sequences:

>Translated_347_residues
MTTQRKAIITGVSGQDGAYLTKLLLSKGYQVTGTYRRTSSVNFWRMEELGVMDNPHLQLVEHDLTDAGSTLRLLDTVQAD
ELYNLAAQSFVGVSFDQPSTTAQVTGVGVLNLLEGIRTVNPQMRFYQASTSEMFGKVQAVPQAEDTPFYPRSPYGVAKLF
AHWSTINYRESYGMFATSGILFNHESPLRGREFVTRKITDTVAKIKLGKADVLELGNLDAKRDWGFALEYVEGMWRMLQA
DEPDTYVLATNRTETVRDFVKMAFAAAGYQLEWTGRDERETGIDISTGKTLIRVNPRFYRPAEVDLLIGCADKAREKLGW
RPQTSLEQLCRMMVEADIARNQNGVSC
>Mature_346_residues
TTQRKAIITGVSGQDGAYLTKLLLSKGYQVTGTYRRTSSVNFWRMEELGVMDNPHLQLVEHDLTDAGSTLRLLDTVQADE
LYNLAAQSFVGVSFDQPSTTAQVTGVGVLNLLEGIRTVNPQMRFYQASTSEMFGKVQAVPQAEDTPFYPRSPYGVAKLFA
HWSTINYRESYGMFATSGILFNHESPLRGREFVTRKITDTVAKIKLGKADVLELGNLDAKRDWGFALEYVEGMWRMLQAD
EPDTYVLATNRTETVRDFVKMAFAAAGYQLEWTGRDERETGIDISTGKTLIRVNPRFYRPAEVDLLIGCADKAREKLGWR
PQTSLEQLCRMMVEADIARNQNGVSC

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=338, Percent_Identity=51.1834319526627, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1788366, Length=349, Percent_Identity=50.1432664756447, Blast_Score=343, Evalue=8e-96,
Organism=Caenorhabditis elegans, GI17539422, Length=344, Percent_Identity=51.7441860465116, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17539424, Length=344, Percent_Identity=51.7441860465116, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI133901790, Length=344, Percent_Identity=51.7441860465116, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI133901786, Length=344, Percent_Identity=51.7441860465116, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI133901788, Length=344, Percent_Identity=51.7441860465116, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17507723, Length=343, Percent_Identity=52.1865889212828, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24158427, Length=338, Percent_Identity=50.2958579881657, Blast_Score=350, Evalue=7e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38974; Mature: 38843

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQRKAIITGVSGQDGAYLTKLLLSKGYQVTGTYRRTSSVNFWRMEELGVMDNPHLQLV
CCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEHHHCCCCCCCCCEEE
EHDLTDAGSTLRLLDTVQADELYNLAAQSFVGVSFDQPSTTAQVTGVGVLNLLEGIRTVN
EHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHCC
PQMRFYQASTSEMFGKVQAVPQAEDTPFYPRSPYGVAKLFAHWSTINYRESYGMFATSGI
CCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCEEECCE
LFNHESPLRGREFVTRKITDTVAKIKLGKADVLELGNLDAKRDWGFALEYVEGMWRMLQA
EECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
DEPDTYVLATNRTETVRDFVKMAFAAAGYQLEWTGRDERETGIDISTGKTLIRVNPRFYR
CCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCEEEEECCCCCC
PAEVDLLIGCADKAREKLGWRPQTSLEQLCRMMVEADIARNQNGVSC
CCCEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTQRKAIITGVSGQDGAYLTKLLLSKGYQVTGTYRRTSSVNFWRMEELGVMDNPHLQLV
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEHHHCCCCCCCCCEEE
EHDLTDAGSTLRLLDTVQADELYNLAAQSFVGVSFDQPSTTAQVTGVGVLNLLEGIRTVN
EHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHCC
PQMRFYQASTSEMFGKVQAVPQAEDTPFYPRSPYGVAKLFAHWSTINYRESYGMFATSGI
CCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCEEECCE
LFNHESPLRGREFVTRKITDTVAKIKLGKADVLELGNLDAKRDWGFALEYVEGMWRMLQA
EECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
DEPDTYVLATNRTETVRDFVKMAFAAAGYQLEWTGRDERETGIDISTGKTLIRVNPRFYR
CCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEECCCEEEEECCCCCC
PAEVDLLIGCADKAREKLGWRPQTSLEQLCRMMVEADIARNQNGVSC
CCCEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8548534; 10984043 [H]