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The map label for this gene is epsB [H]

Identifier: 186475826

GI number: 186475826

Start: 1191585

End: 1193777

Strand: Direct

Name: epsB [H]

Synonym: Bphy_1061

Alternate gene names: 186475826

Gene position: 1191585-1193777 (Clockwise)

Preceding gene: 186475825

Following gene: 186475827

Centisome position: 34.25

GC content: 65.12

Gene sequence:

>2193_bases
ATGCAGGCCAGAACCGAAGAAGAGGACGTGGTCCTCGGCCAACTGCTGCAGGTGATCATCGACGACATCGGGTGGCTGAT
CGGCATCGCGGCGACCATCGTCGCGATGGCCGGCTTCTACTGCTATGTCGCGAAGCCCGTGTATTCGGCCGATGCGCACG
TGCGCGTGGAGGCGTCGGACAACACATCGCAGGCGCTCACGCAGACGCAGACGGGCGCAACGATCTCGACGGGGCAGAAC
ACGATCCCCACCGACGCCGAGATCGAAATGATCAAGAGCCGGGGCGTCGTCGCGCCCGTGGTGCAGCAAATGAAGCTCAA
CTTCTCGGTGACGCCGAAGACGCTGCCCATTCTCGGCAGTCTCGCCGCGCGTGTCGCGACGCCAGGTCAGCCCGCCAAGC
CGTGGCTCGGCCTCGGCAGCTATGCCTGGGGCGGCGAAGTGGCTGCTGTCGATTCGATTGACGTGACGCCCGCGCTCGAA
GGCCAGAAGCTGATCCTCACCGCAATGGACAACGGCCGCTATACGCTGATGACGCCCGACGGCCAGCTGCTGTTGCGCGG
CCAGGCAGGCGAGCCCGCGCAGGGCGGCGGCGTCACGCTGTTCGTGAAGCAGCTGGTGGCCCGTCCGGGCACACAGTTCA
CTGTGATGCGCGCGAACGATCTGGACGCGATCGCCGCGTTCCAGTCCGCGATCAACGTGCAGGAGCAGGGCAAGCAGACG
GGCGTGATCTCGATCTCACTAGAAGACCAAAGCCCCGAGCACGCCGCCGCCGTCGCCAATGCACTTGCGCAGTCGTATCT
GCTCGAGCACATTCGCACCAAGCAGGCCGACGCGAGCCGCATGCTCGACTTCCTGAAGAGCGAAGAGCCGCGCCTCAAGT
CCGATCTCGAACACGCGGAAGCCGCGTTGACGGCGTATCAGAGCAAGAGCGGCTCGATCAACGCGAGCGACGAGGCGAAG
GTCTATCTGGAAGGCAGCGTGCAGTACGAGCAGCAGATTTCGGCCTTGCGTCTGCAGATTTCCCAGCTCGAACAGCGCTA
TGGCGACGATCACCCGATGATCAAGGCCGCGCAGCAGCAGATGGCCGAGCTGCAGGCGCAGCGCACCAAATACGCGGACC
GTTTCCGCGATCTGCCCGCGACGGAAGTGAAGGCCGTGCAGTTGCAGCGCGACGCGAAGGTCGCGGAAGACATCTACGTG
CTGCTGCTCAACCGCGTGCAGGAGCTGTCGGTGCAAAAGGCGGGCACGGGCGGCAACGTGCATATCGTCGACGCGGCGCT
GCGTCCCGGTTCGCCCATCAAGCCGAAGAAGGTGCTGATCTTGTCGGCGGCGACGATTCTCGGGTTGATCGTCGGCACCG
GCTTCGTGTTCCTGCGCCGCAATATGTTCAAGGGCATCGACGACCCGGAGACGGTCGAGCGCGCGTTTCATCTGCCCGTG
TACGGTCTGGTGCCGCTGTCGGCTGAACAGGCGCTGCTCGAAAACGCCGCGGTGCGCGGCGGCGCGCGCGAGCGTGCGGT
GCTCGCGAACGCCAAGCCGAAGGACGTGTGCATCGAAAGCCTGCGCAGCCTGCGCACGTCGATGCAGTTCACGCTGCAGG
ACGCGCGCAATCGCATCGTGATGTTCACGGGCCCGCTGCCGGGCGTCGGCAAGAGCTTCCTGACGATCAACCTGGGCGTG
CTGCTCGCGCATTCGGGCAAGCGGGTGCTGATGATCGACGGCGACATGCGGCGCGGCGCGCTTGAAAAGCATGTGGGCGG
CTCGCCGGACAACGGTCTGTCGGAACTGCTGAGCGGACAGATTTCGCTGGAAGAAGCAGTGCGCGCGACGGAGATCGACA
ACCTCTCGTTCATCTCGTGCGGCCGCCGTCCGCCGAATCCGTCCGAGCTGCTGATGTCGCCTCGCTTGCCTCAGTACCTC
GATGGACTGGCCAAGCGTTACGACGTGATCCTGATCGACACGCCGCCCGTGCTGGCCGTGACCGACGCGTCGATCATCGG
CGGCTATGCGGGTTCGACCTTCTTCGTCGTGCGCTCGGGCATGCACAGCGAAGGCGAGATCGCCGATGCGTTGAAGCGCC
TGCATTCAGCGGGCGTGCACGTGCAGGGCGCGATCTTCAACGCGATGCCGCCGCGCGCGCGCGGCAACTACGACCGCAGC
TATGCGGCCGTACAGGAATACCTGAGCACCTGA

Upstream 100 bases:

>100_bases
GGATGCAACGCGCCGGATGCGGGCGGCGACCCGACCCGGGTCAACCCTGAGCACTCAAGCGAGTATCCAAGTGAGCACTC
AAGCGAATCCACAACTGGCC

Downstream 100 bases:

>100_bases
TGCGCAGCGCGCCTTGCCGGTGCGGCGGGGCGCATGCGCCGTCGACAAGCCACGGAGAGTACAGGATGAAGATAACGGTA
CTGGTGCCGACATACCGCCG

Product: exopolysaccharide tyrosine-protein kinase

Products: ADP; protein tyrosine phosphate [C]

Alternate protein names: EPS I polysaccharide export protein epsB [H]

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASDNTSQALTQTQTGATISTGQN
TIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGSLAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALE
GQKLILTAMDNGRYTLMTPDGQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT
GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAEAALTAYQSKSGSINASDEAK
VYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQMAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYV
LLLNRVQELSVQKAGTGGNVHIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV
YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIVMFTGPLPGVGKSFLTINLGV
LLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYL
DGLAKRYDVILIDTPPVLAVTDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS
YAAVQEYLST

Sequences:

>Translated_730_residues
MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASDNTSQALTQTQTGATISTGQN
TIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGSLAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALE
GQKLILTAMDNGRYTLMTPDGQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT
GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAEAALTAYQSKSGSINASDEAK
VYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQMAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYV
LLLNRVQELSVQKAGTGGNVHIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV
YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIVMFTGPLPGVGKSFLTINLGV
LLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYL
DGLAKRYDVILIDTPPVLAVTDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS
YAAVQEYLST
>Mature_730_residues
MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASDNTSQALTQTQTGATISTGQN
TIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGSLAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALE
GQKLILTAMDNGRYTLMTPDGQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT
GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAEAALTAYQSKSGSINASDEAK
VYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQMAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYV
LLLNRVQELSVQKAGTGGNVHIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV
YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIVMFTGPLPGVGKSFLTINLGV
LLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYL
DGLAKRYDVILIDTPPVLAVTDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS
YAAVQEYLST

Specific function: Probably involved in polymerization and/or export of exopolysaccharide EPS I which functions as a virulence factor. May be involved in an ATP-dependent process in the pathway for EPS I production, possibly export of the trimeric repeat units across the in

COG id: COG3206

COG function: function code M; Uncharacterized protein involved in exopolysaccharide biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the etk/wzc family [H]

Homologues:

Organism=Escherichia coli, GI87082032, Length=721, Percent_Identity=34.8127600554785, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1787216, Length=727, Percent_Identity=31.9119669876204, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR005702
- InterPro:   IPR005700
- InterPro:   IPR003856 [H]

Pfam domain/function: PF01656 CbiA; PF02706 Wzz [H]

EC number: 2.7.1.112 [C]

Molecular weight: Translated: 78693; Mature: 78693

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCCEEEEEECC
NTSQALTQTQTGATISTGQNTIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGS
CCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCEEECCCHHHHHHH
LAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALEGQKLILTAMDNGRYTLMTPD
HHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCEEEEECCC
GQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT
CCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHCCCCC
GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAE
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
AALTAYQSKSGSINASDEAKVYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQ
HHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYVLLLNRVQELSVQKAGTGGNV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
HIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV
EEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCE
YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIV
EEEECCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
MFTGPLPGVGKSFLTINLGVLLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQ
EEECCCCCCCCCEEEEEEEEEEECCCCEEEEECCCHHHCHHHHHCCCCCCCCHHHHHCCC
ISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYLDGLAKRYDVILIDTPPVLAV
CCHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCEEEE
TDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS
ECCHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH
YAAVQEYLST
HHHHHHHHCC
>Mature Secondary Structure
MQARTEEEDVVLGQLLQVIIDDIGWLIGIAATIVAMAGFYCYVAKPVYSADAHVRVEASD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEECCCCEEEEEECC
NTSQALTQTQTGATISTGQNTIPTDAEIEMIKSRGVVAPVVQQMKLNFSVTPKTLPILGS
CCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCEEECCCHHHHHHH
LAARVATPGQPAKPWLGLGSYAWGGEVAAVDSIDVTPALEGQKLILTAMDNGRYTLMTPD
HHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCEEEEECCC
GQLLLRGQAGEPAQGGGVTLFVKQLVARPGTQFTVMRANDLDAIAAFQSAINVQEQGKQT
CCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHCCCCC
GVISISLEDQSPEHAAAVANALAQSYLLEHIRTKQADASRMLDFLKSEEPRLKSDLEHAE
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
AALTAYQSKSGSINASDEAKVYLEGSVQYEQQISALRLQISQLEQRYGDDHPMIKAAQQQ
HHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MAELQAQRTKYADRFRDLPATEVKAVQLQRDAKVAEDIYVLLLNRVQELSVQKAGTGGNV
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
HIVDAALRPGSPIKPKKVLILSAATILGLIVGTGFVFLRRNMFKGIDDPETVERAFHLPV
EEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCE
YGLVPLSAEQALLENAAVRGGARERAVLANAKPKDVCIESLRSLRTSMQFTLQDARNRIV
EEEECCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
MFTGPLPGVGKSFLTINLGVLLAHSGKRVLMIDGDMRRGALEKHVGGSPDNGLSELLSGQ
EEECCCCCCCCCEEEEEEEEEEECCCCEEEEECCCHHHCHHHHHCCCCCCCCHHHHHCCC
ISLEEAVRATEIDNLSFISCGRRPPNPSELLMSPRLPQYLDGLAKRYDVILIDTPPVLAV
CCHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCEEEE
TDASIIGGYAGSTFFVVRSGMHSEGEIADALKRLHSAGVHVQGAIFNAMPPRARGNYDRS
ECCHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH
YAAVQEYLST
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; a protein tyrosine [C]

Specific reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]