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The map label for this gene is pncA [H]

Identifier: 186475794

GI number: 186475794

Start: 1160748

End: 1161374

Strand: Direct

Name: pncA [H]

Synonym: Bphy_1029

Alternate gene names: 186475794

Gene position: 1160748-1161374 (Clockwise)

Preceding gene: 186475793

Following gene: 186475795

Centisome position: 33.36

GC content: 66.99

Gene sequence:

>627_bases
ATGAAAGATGCCAATGAAGTTCTGCTCGTCGTCGACGTGCAGAACGACTTCATGCCGGGCGGTGCGCTCGCCGTCGCGCG
CGGCGACGAAATCGTGCCGCTCGTCAACCGGCTTGCACGCCGTTTCAGTCATGTGGTGCTGACGCAGGACTGGCATCCGC
CGTCGCATGTCTCGTTCGCTTCGAATCACGCGGGACGCCAGCCGTTCGAGACGATGACGCTGCCCTATGGCGAGCAGGTG
CTGTGGCCGGCGCACTGCGTGCAGAACACGCCGGGCGCCGCGCTGCACGCGGACCTCGACATTCCGCATGCGCGCGCCGT
GATCCGCAAGGGGCATCATGCACGCGTCGACAGCTATTCGGCGTTTCTCGAAGCCGATCGCACGACGCCGACGGGTCTCG
CGGGATATCTGCGCGATACGGGCGTGACGCGCGTGTGGTGCTGCGGGCTCGCCACCGACTATTGCGTTGCGTGGTCGGCG
CTCGATGCGCGCGCAGCGGGCTTCGCGGTGGCCGTCATCGAGGACGCGACCCGCGCCATCGATCTGAACGGTTCGCTCGA
CAACGCGTGGCACGCGATGCGCGCGGCAGGCGTCGAACGCGTACAGGCGGCGGATGTTCCTGCCTGA

Upstream 100 bases:

>100_bases
GCAGCCGAACGGGGACGCGTGGCAGATCGCGGCCGGCATCGTCGAGCGCGCGGCGCAAGGGCGCGGATTCAACAGCTGAT
CGAGCCGGACAGGATTTGCG

Downstream 100 bases:

>100_bases
GCAGCGCGAACCGCACACATTCAATTTAAAGAGTTAATCGTTACGTCGGACGGGAGCCAGCGCTCAAGCCACCGCTTCCA
TCGACATTGGAGAGATGCAT

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKDANEVLLVVDVQNDFMPGGALAVARGDEIVPLVNRLARRFSHVVLTQDWHPPSHVSFASNHAGRQPFETMTLPYGEQV
LWPAHCVQNTPGAALHADLDIPHARAVIRKGHHARVDSYSAFLEADRTTPTGLAGYLRDTGVTRVWCCGLATDYCVAWSA
LDARAAGFAVAVIEDATRAIDLNGSLDNAWHAMRAAGVERVQAADVPA

Sequences:

>Translated_208_residues
MKDANEVLLVVDVQNDFMPGGALAVARGDEIVPLVNRLARRFSHVVLTQDWHPPSHVSFASNHAGRQPFETMTLPYGEQV
LWPAHCVQNTPGAALHADLDIPHARAVIRKGHHARVDSYSAFLEADRTTPTGLAGYLRDTGVTRVWCCGLATDYCVAWSA
LDARAAGFAVAVIEDATRAIDLNGSLDNAWHAMRAAGVERVQAADVPA
>Mature_208_residues
MKDANEVLLVVDVQNDFMPGGALAVARGDEIVPLVNRLARRFSHVVLTQDWHPPSHVSFASNHAGRQPFETMTLPYGEQV
LWPAHCVQNTPGAALHADLDIPHARAVIRKGHHARVDSYSAFLEADRTTPTGLAGYLRDTGVTRVWCCGLATDYCVAWSA
LDARAAGFAVAVIEDATRAIDLNGSLDNAWHAMRAAGVERVQAADVPA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=203, Percent_Identity=44.3349753694581, Blast_Score=151, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI193206735, Length=216, Percent_Identity=34.7222222222222, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=216, Percent_Identity=34.7222222222222, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71999339, Length=215, Percent_Identity=33.4883720930233, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=191, Percent_Identity=34.5549738219895, Blast_Score=116, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648247, Length=198, Percent_Identity=36.8686868686869, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22469; Mature: 22469

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDANEVLLVVDVQNDFMPGGALAVARGDEIVPLVNRLARRFSHVVLTQDWHPPSHVSFA
CCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEEEECCCCCCCCEEC
SNHAGRQPFETMTLPYGEQVLWPAHCVQNTPGAALHADLDIPHARAVIRKGHHARVDSYS
CCCCCCCCCCEEECCCCCCEECHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCCCCCHHH
AFLEADRTTPTGLAGYLRDTGVTRVWCCGLATDYCVAWSALDARAAGFAVAVIEDATRAI
HHHCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEEEECCCEEE
DLNGSLDNAWHAMRAAGVERVQAADVPA
ECCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKDANEVLLVVDVQNDFMPGGALAVARGDEIVPLVNRLARRFSHVVLTQDWHPPSHVSFA
CCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEEEECCCCCCCCEEC
SNHAGRQPFETMTLPYGEQVLWPAHCVQNTPGAALHADLDIPHARAVIRKGHHARVDSYS
CCCCCCCCCCEEECCCCCCEECHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCCCCCHHH
AFLEADRTTPTGLAGYLRDTGVTRVWCCGLATDYCVAWSALDARAAGFAVAVIEDATRAI
HHHCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEEEECCCEEE
DLNGSLDNAWHAMRAAGVERVQAADVPA
ECCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]