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The map label for this gene is tesA [H]

Identifier: 186475782

GI number: 186475782

Start: 1141937

End: 1142581

Strand: Reverse

Name: tesA [H]

Synonym: Bphy_1017

Alternate gene names: 186475782

Gene position: 1142581-1141937 (Counterclockwise)

Preceding gene: 186475784

Following gene: 186475781

Centisome position: 32.84

GC content: 64.03

Gene sequence:

>645_bases
ATGCCCGCTCATGCAACGGGCGACACGAGCGCGGGTGCGGCGGCAGCAACCCGAAGCGCGGCAGGCAGTAACGCACAATC
GTCGAAGCCGACCATCGTCGTACTTGGCGACAGCCTCTCCGCCGAATACGGCTTGCCCCGGGACACGGGCTGGGTTTCCT
TGATGCGCCAGCGACTGTCGAATGCGCAGATCGATTATAACGTTGCGAATGCGAGCATCAGCGGCGACACCACGAGCGGC
GGGCGCGCCCGCTTGCCCGCGCTGATGCAACGGCTCAAGCCGGCCATCGTAATCGTCGAGCTCGGCGCCAACGACGCGCT
GCGCGGTGTCCCGCTGAGCACGACGGAAGACAACCTGCGCACGATCGTCGAACAGGCCCAGCAAGGACACGCGAAGGTTC
TGCTCGTCGGGATGTACGTGCCGCCCAACTACGGGCCCGACTACACGCAGAAGTTCCACGCCCTCTATGGACAACTGTCG
AAGCAGTTGCGCGTGCCGCTCGTCCCATTCCTCCTCGCCGGAATCGAAGACAAGCCAGACATGTTCCAGTCTGATCAGAT
TCATCCGACGCAACGGGCACAGCCCCTGCTACTGGACAACGTGTGGCCGGCGCTGAAGCCGCTGCTCCACACCTCGCCCC
AATGA

Upstream 100 bases:

>100_bases
CTTTGACATGGTGAAGCGTAGATTGAAAGTACGCGCCATTTCGTTCGTGACAGCGCTGACGGCAGCCTGTTTCCCTATCC
TGCTCGCCGCGACTTTCTCG

Downstream 100 bases:

>100_bases
GCGCGTTGCCGAGAAAGAGACGGCGCGCGCGGACAACCGGTTTTCCAAACGATTGGGAAACGTGACCAGGTACGGTCGGT
TCAGATAGGTGATTGCATGA

Product: lysophospholipase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPAHATGDTSAGAAAATRSAAGSNAQSSKPTIVVLGDSLSAEYGLPRDTGWVSLMRQRLSNAQIDYNVANASISGDTTSG
GRARLPALMQRLKPAIVIVELGANDALRGVPLSTTEDNLRTIVEQAQQGHAKVLLVGMYVPPNYGPDYTQKFHALYGQLS
KQLRVPLVPFLLAGIEDKPDMFQSDQIHPTQRAQPLLLDNVWPALKPLLHTSPQ

Sequences:

>Translated_214_residues
MPAHATGDTSAGAAAATRSAAGSNAQSSKPTIVVLGDSLSAEYGLPRDTGWVSLMRQRLSNAQIDYNVANASISGDTTSG
GRARLPALMQRLKPAIVIVELGANDALRGVPLSTTEDNLRTIVEQAQQGHAKVLLVGMYVPPNYGPDYTQKFHALYGQLS
KQLRVPLVPFLLAGIEDKPDMFQSDQIHPTQRAQPLLLDNVWPALKPLLHTSPQ
>Mature_213_residues
PAHATGDTSAGAAAATRSAAGSNAQSSKPTIVVLGDSLSAEYGLPRDTGWVSLMRQRLSNAQIDYNVANASISGDTTSGG
RARLPALMQRLKPAIVIVELGANDALRGVPLSTTEDNLRTIVEQAQQGHAKVLLVGMYVPPNYGPDYTQKFHALYGQLSK
QLRVPLVPFLLAGIEDKPDMFQSDQIHPTQRAQPLLLDNVWPALKPLLHTSPQ

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=180, Percent_Identity=42.2222222222222, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 22913; Mature: 22782

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHATGDTSAGAAAATRSAAGSNAQSSKPTIVVLGDSLSAEYGLPRDTGWVSLMRQRLS
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
NAQIDYNVANASISGDTTSGGRARLPALMQRLKPAIVIVELGANDALRGVPLSTTEDNLR
CCEEEEEECCCEECCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHH
TIVEQAQQGHAKVLLVGMYVPPNYGPDYTQKFHALYGQLSKQLRVPLVPFLLAGIEDKPD
HHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
MFQSDQIHPTQRAQPLLLDNVWPALKPLLHTSPQ
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PAHATGDTSAGAAAATRSAAGSNAQSSKPTIVVLGDSLSAEYGLPRDTGWVSLMRQRLS
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHC
NAQIDYNVANASISGDTTSGGRARLPALMQRLKPAIVIVELGANDALRGVPLSTTEDNLR
CCEEEEEECCCEECCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHH
TIVEQAQQGHAKVLLVGMYVPPNYGPDYTQKFHALYGQLSKQLRVPLVPFLLAGIEDKPD
HHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCC
MFQSDQIHPTQRAQPLLLDNVWPALKPLLHTSPQ
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]