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The map label for this gene is ispF

Identifier: 186475764

GI number: 186475764

Start: 1121567

End: 1122046

Strand: Direct

Name: ispF

Synonym: Bphy_0999

Alternate gene names: 186475764

Gene position: 1121567-1122046 (Clockwise)

Preceding gene: 186475763

Following gene: 186475769

Centisome position: 32.24

GC content: 64.58

Gene sequence:

>480_bases
ATGGATTTGAGAATCGGACAAGGCTATGACGTTCACGCACTGGTGCCGGGACGACCGCTCATCATCGGGGGCGTGACGAT
TCCCTACGAGCGCGGACTGCTCGGCCATTCGGATGCCGACGTGCTGCTGCACGCGATTACCGACGCATTGTTCGGCGCCG
CCGCGCTCGGCGACATCGGCCGTCATTTCCCGGATACCGCGACGGAATTCAAAGGCGCGAACAGCCGCGTACTGCTGCGC
GAATGCGCGGCGCGCATCGCAAGGGCGGGCTTCACGATCCAGAACGTCGACAGCACCGTGATCGCGCAGGCGCCGAAGCT
CGCGCCGCATATCGACGGGATGCGCGCGAATATCGCCGAAGACCTCAACCTGCCCATCGACCGCGTCAACGTAAAGGCGA
AGACGAACGAGAAGCTCGGCTATCTCGGACGAGGCGAGGGCATCGAAGCGCAGGCGGCCGCGCTGCTGGTGCGCGTCTGA

Upstream 100 bases:

>100_bases
AGCTATCCTCGGACGCGGCGCGGCGGGCTGACTCACGCGCTCATCGCGTCGGCTTCGCGCCGACCATAACGCTTTCACGA
AACAACGGAACCTCACGCAT

Downstream 100 bases:

>100_bases
ATTTTCGCGGGTCAGGAAGCATATCGAGCGCCCGCAGAAGCGACAACGCCACGCAGATCGCGTGGCGTTTTTCACAAATC
GAAGCTTCGCTCACTTGTCT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MDLRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFPDTATEFKGANSRVLLR
ECAARIARAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAEDLNLPIDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVRV

Sequences:

>Translated_159_residues
MDLRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFPDTATEFKGANSRVLLR
ECAARIARAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAEDLNLPIDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVRV
>Mature_159_residues
MDLRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFPDTATEFKGANSRVLLR
ECAARIARAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAEDLNLPIDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVRV

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=64.7435897435898, Blast_Score=187, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BURP8 (B2JGK3)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857234.1
- ProteinModelPortal:   B2JGK3
- SMR:   B2JGK3
- GeneID:   6242497
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0999
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16882; Mature: 16882

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIG
CCCCCCCCCCEEEECCCCEEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RHFPDTATEFKGANSRVLLRECAARIARAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAE
HCCCCCHHHHCCCCCEEHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCHHHHHHH
DLNLPIDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVRV
HCCCCHHHEEEEECCCCCCCCCCCCCCCCHHHHEEEEEC
>Mature Secondary Structure
MDLRIGQGYDVHALVPGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIG
CCCCCCCCCCEEEECCCCEEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RHFPDTATEFKGANSRVLLRECAARIARAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAE
HCCCCCHHHHCCCCCEEHHHHHHHHHHHCCCEEECCCCHHHHCCCCCCCCCCCHHHHHHH
DLNLPIDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVRV
HCCCCHHHEEEEECCCCCCCCCCCCCCCCHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA