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The map label for this gene is pdxJ [H]

Identifier: 186475609

GI number: 186475609

Start: 943356

End: 944120

Strand: Direct

Name: pdxJ [H]

Synonym: Bphy_0844

Alternate gene names: 186475609

Gene position: 943356-944120 (Clockwise)

Preceding gene: 186475608

Following gene: 186475610

Centisome position: 27.11

GC content: 64.97

Gene sequence:

>765_bases
ATGAGCTTCTTCCTGACTTCTCCGAATGTGATCGACCTCGGCGTGAACATCGATCACGTCGCCACGCTGCGCAACGCGCG
CGGCACGTCCTATCCCGATCCGATCCGCGCCGCGCTTCAGGCGGAAGAAGCGGGCGCCGATGCGATCACGCTGCATCTGC
GTGAAGACCGCCGCCACATCGTCGACGCCGACGTGCGCGCGTTGCGTCCGCAACTAACGACGCGCATGAACCTCGAATGC
GCGGTGACGCAGGAAATGCTCGACATCGCGTGCGAAGTGCAGCCGCATGATGTGTGTCTCGTGCCGGAAAAGCGCCAGGA
GCTGACGACGGAAGGCGGCCTCGATGTCGCCGGGCACTTCGAGGCGGTACGCGCCGCGTGCAAGCAACTGGCGGACGCCG
GCTCGCGCGTGTCGCTCTTCATCGATGCCGACGAGGCCCAGATTCGCGCCGCCCACGAAGCAGGTGCGCCCGTTATCGAA
CTTCACACGGGAGCCTACGCGGAAGCGCACGACGCAAGCGAGCAGCAGCGCGAATACGAACGCATTGTGCGCGGCGTCGA
GTTCGGCGCGTCGCTGGGCCTGAAGGTGAACGCAGGTCACGGCCTTCACTACACGAACGTCCAGCAGATCGCGGCGATCG
AAGGCATCGTCGAACTGAATATCGGCCACGCGATCGTCGCACATGCGATCTTCGCGGGCTGGGACAACGCCGTGCGCGAG
ATGAAGGCGATCATGGTCGCCGCGCGTCTTGCGGCGCGCGCATAA

Upstream 100 bases:

>100_bases
CCATCGCGCAAAGCAAGACGCTGATGCGCTTTTTGCTCAACATCTATCTGGGCGGCACGCCGCTCGCGACGCGCCAGATC
CTGATCGACCTGCAGAACTT

Downstream 100 bases:

>100_bases
CCAGGCAGACAAACGGCACGCGTATGGCGATCTACGGCATCGGCACCGACATCGTTCAGGTCAGCCGCGTCGCGGCAGTG
ATGCAGCGGACCAACGGGCG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSFFLTSPNVIDLGVNIDHVATLRNARGTSYPDPIRAALQAEEAGADAITLHLREDRRHIVDADVRALRPQLTTRMNLEC
AVTQEMLDIACEVQPHDVCLVPEKRQELTTEGGLDVAGHFEAVRAACKQLADAGSRVSLFIDADEAQIRAAHEAGAPVIE
LHTGAYAEAHDASEQQREYERIVRGVEFGASLGLKVNAGHGLHYTNVQQIAAIEGIVELNIGHAIVAHAIFAGWDNAVRE
MKAIMVAARLAARA

Sequences:

>Translated_254_residues
MSFFLTSPNVIDLGVNIDHVATLRNARGTSYPDPIRAALQAEEAGADAITLHLREDRRHIVDADVRALRPQLTTRMNLEC
AVTQEMLDIACEVQPHDVCLVPEKRQELTTEGGLDVAGHFEAVRAACKQLADAGSRVSLFIDADEAQIRAAHEAGAPVIE
LHTGAYAEAHDASEQQREYERIVRGVEFGASLGLKVNAGHGLHYTNVQQIAAIEGIVELNIGHAIVAHAIFAGWDNAVRE
MKAIMVAARLAARA
>Mature_253_residues
SFFLTSPNVIDLGVNIDHVATLRNARGTSYPDPIRAALQAEEAGADAITLHLREDRRHIVDADVRALRPQLTTRMNLECA
VTQEMLDIACEVQPHDVCLVPEKRQELTTEGGLDVAGHFEAVRAACKQLADAGSRVSLFIDADEAQIRAAHEAGAPVIEL
HTGAYAEAHDASEQQREYERIVRGVEFGASLGLKVNAGHGLHYTNVQQIAAIEGIVELNIGHAIVAHAIFAGWDNAVREM
KAIMVAARLAARA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=237, Percent_Identity=67.5105485232068, Blast_Score=318, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 27504; Mature: 27373

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFLTSPNVIDLGVNIDHVATLRNARGTSYPDPIRAALQAEEAGADAITLHLREDRRHI
CEEEECCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHH
VDADVRALRPQLTTRMNLECAVTQEMLDIACEVQPHDVCLVPEKRQELTTEGGLDVAGHF
HHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCHHHHH
EAVRAACKQLADAGSRVSLFIDADEAQIRAAHEAGAPVIELHTGAYAEAHDASEQQREYE
HHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHH
RIVRGVEFGASLGLKVNAGHGLHYTNVQQIAAIEGIVELNIGHAIVAHAIFAGWDNAVRE
HHHHHHHCCCCCCEEEECCCCCEECCHHHHHHHHHHEEECCCHHHHHHHHHHCHHHHHHH
MKAIMVAARLAARA
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SFFLTSPNVIDLGVNIDHVATLRNARGTSYPDPIRAALQAEEAGADAITLHLREDRRHI
EEEECCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHH
VDADVRALRPQLTTRMNLECAVTQEMLDIACEVQPHDVCLVPEKRQELTTEGGLDVAGHF
HHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCCCHHHHH
EAVRAACKQLADAGSRVSLFIDADEAQIRAAHEAGAPVIELHTGAYAEAHDASEQQREYE
HHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHH
RIVRGVEFGASLGLKVNAGHGLHYTNVQQIAAIEGIVELNIGHAIVAHAIFAGWDNAVRE
HHHHHHHCCCCCCEEEECCCCCEECCHHHHHHHHHHEEECCCHHHHHHHHHHCHHHHHHH
MKAIMVAARLAARA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA