Click here to switch to the map view.

The map label for this gene is gph [C]

Identifier: 186475585

GI number: 186475585

Start: 921491

End: 922150

Strand: Direct

Name: gph [C]

Synonym: Bphy_0820

Alternate gene names: 186475585

Gene position: 921491-922150 (Clockwise)

Preceding gene: 186475584

Following gene: 186475586

Centisome position: 26.49

GC content: 64.55

Gene sequence:

>660_bases
ATGGCTCGACAGCAATTTGATCTGATCGTCTTCGATTGGGACGGTACGTTGATGGATTCGACCGTCCATATCACGCGCAG
CATCCAGGCGGCGTGCCGCGACCTCGGGTTGCCGGTGCCCGCCGACGAGGCCGCGAGTTTCGTGATCGGTCTTGGCTTGC
GCGACGCGCTCCAGATCGCGGCGCCGACGCTCGATCCGGCGGATTATCCGCGGCTTGCCGAGCGCTATCGCTTTCACTAT
CTGGTGAAGGATCAGACGACCGAGCTGTTTGCGGGCGTGCGCGAGATGCTGGCGGACCTGCGAGATCAGGGCTATCTGCT
CGCAATCGCGACTGGCAAGAGCCGCGTCGGCCTCAACCGCGCGCTGGATCAGGTGCGACTGACGAGCCTGTTCGACGGCA
CCCGCTGTGCCGACGAAACGTTCTCGAAACCTCATCCCGCCATGCTGCACGAGCTGACACGCGAACTGGGGCAGGATCCG
GTGCGCACATTGATGATCGGCGATACCACGCACGACCTGCAGATGGCGATCAACGCGGGCGTGGCGGGCGTCGGCGTCAC
TTATGGCGCGCATCCCGCCCGTTCGCTGGCGGCGCTGGAACCGAAATTCGTCGCGGACAGCATCGCATCGCTGTCGGGCT
GGCTGCGGGAGCACGCATGA

Upstream 100 bases:

>100_bases
GCGCCGTTGCCGGCGGAGTGCAAGCGTTTCCTCGCGCAGCTCGCCGAAATGGCTGGCCGGACGGCGGAGCACACACAATC
ACATAACGAGACGGGAACGC

Downstream 100 bases:

>100_bases
CGGACGCTGCGCTCGAACCGGTGCGCGTGTGCGCGTCGGAGGAACTCGTGGACGGCGGCGCGGGCGTTCGCATCGACGCG
ACGCACGCAGGCGGCGATGC

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MARQQFDLIVFDWDGTLMDSTVHITRSIQAACRDLGLPVPADEAASFVIGLGLRDALQIAAPTLDPADYPRLAERYRFHY
LVKDQTTELFAGVREMLADLRDQGYLLAIATGKSRVGLNRALDQVRLTSLFDGTRCADETFSKPHPAMLHELTRELGQDP
VRTLMIGDTTHDLQMAINAGVAGVGVTYGAHPARSLAALEPKFVADSIASLSGWLREHA

Sequences:

>Translated_219_residues
MARQQFDLIVFDWDGTLMDSTVHITRSIQAACRDLGLPVPADEAASFVIGLGLRDALQIAAPTLDPADYPRLAERYRFHY
LVKDQTTELFAGVREMLADLRDQGYLLAIATGKSRVGLNRALDQVRLTSLFDGTRCADETFSKPHPAMLHELTRELGQDP
VRTLMIGDTTHDLQMAINAGVAGVGVTYGAHPARSLAALEPKFVADSIASLSGWLREHA
>Mature_218_residues
ARQQFDLIVFDWDGTLMDSTVHITRSIQAACRDLGLPVPADEAASFVIGLGLRDALQIAAPTLDPADYPRLAERYRFHYL
VKDQTTELFAGVREMLADLRDQGYLLAIATGKSRVGLNRALDQVRLTSLFDGTRCADETFSKPHPAMLHELTRELGQDPV
RTLMIGDTTHDLQMAINAGVAGVGVTYGAHPARSLAALEPKFVADSIASLSGWLREHA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=226, Percent_Identity=28.3185840707965, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23973; Mature: 23841

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQQFDLIVFDWDGTLMDSTVHITRSIQAACRDLGLPVPADEAASFVIGLGLRDALQIA
CCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHH
APTLDPADYPRLAERYRFHYLVKDQTTELFAGVREMLADLRDQGYLLAIATGKSRVGLNR
CCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
ALDQVRLTSLFDGTRCADETFSKPHPAMLHELTRELGQDPVRTLMIGDTTHDLQMAINAG
HHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHCC
VAGVGVTYGAHPARSLAALEPKFVADSIASLSGWLREHA
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARQQFDLIVFDWDGTLMDSTVHITRSIQAACRDLGLPVPADEAASFVIGLGLRDALQIA
CCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHH
APTLDPADYPRLAERYRFHYLVKDQTTELFAGVREMLADLRDQGYLLAIATGKSRVGLNR
CCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
ALDQVRLTSLFDGTRCADETFSKPHPAMLHELTRELGQDPVRTLMIGDTTHDLQMAINAG
HHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHCC
VAGVGVTYGAHPARSLAALEPKFVADSIASLSGWLREHA
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA