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The map label for this gene is pcrA [H]

Identifier: 186475566

GI number: 186475566

Start: 899172

End: 901424

Strand: Direct

Name: pcrA [H]

Synonym: Bphy_0801

Alternate gene names: 186475566

Gene position: 899172-901424 (Clockwise)

Preceding gene: 186475565

Following gene: 186475573

Centisome position: 25.84

GC content: 65.56

Gene sequence:

>2253_bases
GTGGTCGCGTGTTGTATGGATATACAGTATGCGTCGCGTTCACTCTCCTATCCGCCGATCGTGCTTTCCGTCGCCGATTC
CCCGTCTTCATCGTCGCCTGCGTCGCCTGATTCGTCCGCGTCGGCGGCGGCTGCCGACTGGCTCGCAAAGCTGAACGACG
CGCAGCGCGAAGCCGTCGAATACGGCGCCGACCGCGTGGCGCATCCGTCCGGGGCGCTCCTCGTCATCGCGGGCGCGGGC
TCGGGCAAGACCAACACGCTTGCGCATCGAGTCGCGAATCTCGTGGTCAAGGGCGTCGATCCGCGCCGCATTCTGCTGCT
GACGTTCTCGCGTCGGGCAGCGCTCGAAATGACGCGGCGCGTCACGCGCATCGCCGGCATGGCGCTGGGCACGCGCGGGG
CGCTCGCGCAGGGCCTGACATGGTCCGGCACGTTCCACAGCGTCGGCGCGCGCCTGTTGCGCGAGTATGCCGATCTGATC
GGCCTGTCGCCTAGCTTCACGATCAACGACCGCGAAGATTCTGCCGATCTGCTGAATCTCGTGCGCCACGAACTCGGCTT
TTCGGCAAAGGAAAAGCGCTTTCCGTCGAAGTCGGCGTGCTTCGCGATTTATTCGCGTGTCGTGAACACGGGCGCGTCGC
TGGCGGACGTGCTCAACAGCGCGTTTCCCTGGTGCCGCGAATGGGAAGCCGATCTGCGCATGCTCTTTGCGGCCTACGTC
GACGCGAAGCAGAAGCAGAGCGTGCTCGACTACGACGACCTGCTGCTCTACTGGTCGCACATGGCGGCGGAACCGGCGAT
TGCCGCCGACCTGTCCGGCCGCTTCGATCACGTGCTCGTCGACGAATACCAGGACACGAACCGTCTGCAGGCGTCGATCC
TGCTCGCCCTGAAGCCCGATGGGCGCGGCCTGACGGTGGTCGGCGACGACGCGCAATCCATCTATTCGTTTCGCGGCGCG
ACCGTGCGCAACATTCTCGACTTCCCGGCGCATTTCGACCCACCCGCGAGGCAGGTCACGCTGGAGCGCAACTACCGCTC
GACGCAGCCCATTTTGGAAGCATCGAACGCCGTGATCGGCCTCGCGTCGGAGCGGTACACGAAGAATCTGTGGACCGACA
AGCCGTCGGCACAACGGCCGCATCTCGTCACCGTCGCCGACGACGCCGATCAGGCGCGCTACGTCGTCGAACAGGTGCTC
GCGGCGCGGGAAGCCGGGATGAAGCTGAAGTCGCAGGCGGTCCTCTTTCGCGCCGCACATCATAGCGCGACGCTGGAGAT
CGAACTGACGCGGCGGAATATTCCGTTCGTGAAGTTCGGCGGCCTGAAGTTTCTCGATTCCGTTCACGTGAAGGACGTGT
TGTCCGTGCTGCGCTGGGCAGAGAATCCGCGCGATCGCGTGGCGGGCTTTCGCGTCGTGCAGTTGCTGCCGGGCGTCGGG
CCCGCGACGGCCGCGCGCGTGCTCGACGATATCGCCGCGCGAGCCGATGCGCTCGGCTCAGCCGGCGGGCTGGAGGCCGC
GCATGGCGTGTCCACGTGCGCAGCGGGCGCGCTTACCGCGTTCGCACCGCCTGCGCGCACACTCGAAGACTGGCATCCGT
TTGTGGCGATGATGGCGTCCGTCTGCGGGCGCCAGACGCCGTGGCCCGCCGAATTCGAGATGGTGCGCCGCTGGTACGAG
CCGCATCTTCAGCGCAATCACGAGGACGCATCCATTCGGCAGGCCGATCTTGTGCAGATGGAGAGCATCGCGGGCACTTA
CGCGTCGCGCGAGCGCTTCCTGACCGAGCTGACGCTCGATCCGCCCGACGCCACCAGCGACGAATCTGGCGTCCCCCTGA
TCGACGAGGACTATCTGATCCTGTCCACCATCCATTCGGCGAAAGGGCAGGAGTGGCGCAATGTGTTCGTGCTCAATGGC
GTGGACGGCTGCATTCCGTCGGATCTCGGCACGGGCAGCGAGGAAGAAATCGACGAGGAGCGGCGCCTGCTGTACGTGGC
GATGACGCGCGCGAAAGAAGATTTGCATATCGTGATGCCGCAGCGCTTCTACGTGCACAACCAGACGCATCTCGGCGACC
GGCACGTGTGGGCGTCGCGTACGCGATTCATCCCGCCGCATCTGCTGCCGCTGTTCGATTCGCATGCGTGGCCGCCCGCG
CCCGTCGCGTCAGCGCCGACGGAGATGGGGCTGGCCGCCGCTGCGCAGGCCAAAATAGAAATCGCGGCGAAGCTCAGAAA
GATGTGGGACTGA

Upstream 100 bases:

>100_bases
CCGGACGGTTTCGCGCCGTTCGGCGCACCAGCCCGGCACGCGCCGGGCTTTTCTTTGCGCGTATCACGAACAGTACGCGA
TAGAGGAAAATGGGGAGCTT

Downstream 100 bases:

>100_bases
GCGCGCCGCGATCGGGCGGCGCCCGATGCGCACGCCGTTCTCTAAGGTTCCGACACAGAACTAGCGCGATTGCCGCTGTT
GCGGTTGCTGCGGCCGCGGA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MVACCMDIQYASRSLSYPPIVLSVADSPSSSSPASPDSSASAAAADWLAKLNDAQREAVEYGADRVAHPSGALLVIAGAG
SGKTNTLAHRVANLVVKGVDPRRILLLTFSRRAALEMTRRVTRIAGMALGTRGALAQGLTWSGTFHSVGARLLREYADLI
GLSPSFTINDREDSADLLNLVRHELGFSAKEKRFPSKSACFAIYSRVVNTGASLADVLNSAFPWCREWEADLRMLFAAYV
DAKQKQSVLDYDDLLLYWSHMAAEPAIAADLSGRFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYSFRGA
TVRNILDFPAHFDPPARQVTLERNYRSTQPILEASNAVIGLASERYTKNLWTDKPSAQRPHLVTVADDADQARYVVEQVL
AAREAGMKLKSQAVLFRAAHHSATLEIELTRRNIPFVKFGGLKFLDSVHVKDVLSVLRWAENPRDRVAGFRVVQLLPGVG
PATAARVLDDIAARADALGSAGGLEAAHGVSTCAAGALTAFAPPARTLEDWHPFVAMMASVCGRQTPWPAEFEMVRRWYE
PHLQRNHEDASIRQADLVQMESIAGTYASRERFLTELTLDPPDATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNG
VDGCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLHIVMPQRFYVHNQTHLGDRHVWASRTRFIPPHLLPLFDSHAWPPA
PVASAPTEMGLAAAAQAKIEIAAKLRKMWD

Sequences:

>Translated_750_residues
MVACCMDIQYASRSLSYPPIVLSVADSPSSSSPASPDSSASAAAADWLAKLNDAQREAVEYGADRVAHPSGALLVIAGAG
SGKTNTLAHRVANLVVKGVDPRRILLLTFSRRAALEMTRRVTRIAGMALGTRGALAQGLTWSGTFHSVGARLLREYADLI
GLSPSFTINDREDSADLLNLVRHELGFSAKEKRFPSKSACFAIYSRVVNTGASLADVLNSAFPWCREWEADLRMLFAAYV
DAKQKQSVLDYDDLLLYWSHMAAEPAIAADLSGRFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYSFRGA
TVRNILDFPAHFDPPARQVTLERNYRSTQPILEASNAVIGLASERYTKNLWTDKPSAQRPHLVTVADDADQARYVVEQVL
AAREAGMKLKSQAVLFRAAHHSATLEIELTRRNIPFVKFGGLKFLDSVHVKDVLSVLRWAENPRDRVAGFRVVQLLPGVG
PATAARVLDDIAARADALGSAGGLEAAHGVSTCAAGALTAFAPPARTLEDWHPFVAMMASVCGRQTPWPAEFEMVRRWYE
PHLQRNHEDASIRQADLVQMESIAGTYASRERFLTELTLDPPDATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNG
VDGCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLHIVMPQRFYVHNQTHLGDRHVWASRTRFIPPHLLPLFDSHAWPPA
PVASAPTEMGLAAAAQAKIEIAAKLRKMWD
>Mature_750_residues
MVACCMDIQYASRSLSYPPIVLSVADSPSSSSPASPDSSASAAAADWLAKLNDAQREAVEYGADRVAHPSGALLVIAGAG
SGKTNTLAHRVANLVVKGVDPRRILLLTFSRRAALEMTRRVTRIAGMALGTRGALAQGLTWSGTFHSVGARLLREYADLI
GLSPSFTINDREDSADLLNLVRHELGFSAKEKRFPSKSACFAIYSRVVNTGASLADVLNSAFPWCREWEADLRMLFAAYV
DAKQKQSVLDYDDLLLYWSHMAAEPAIAADLSGRFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYSFRGA
TVRNILDFPAHFDPPARQVTLERNYRSTQPILEASNAVIGLASERYTKNLWTDKPSAQRPHLVTVADDADQARYVVEQVL
AAREAGMKLKSQAVLFRAAHHSATLEIELTRRNIPFVKFGGLKFLDSVHVKDVLSVLRWAENPRDRVAGFRVVQLLPGVG
PATAARVLDDIAARADALGSAGGLEAAHGVSTCAAGALTAFAPPARTLEDWHPFVAMMASVCGRQTPWPAEFEMVRRWYE
PHLQRNHEDASIRQADLVQMESIAGTYASRERFLTELTLDPPDATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNG
VDGCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLHIVMPQRFYVHNQTHLGDRHVWASRTRFIPPHLLPLFDSHAWPPA
PVASAPTEMGLAAAAQAKIEIAAKLRKMWD

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=664, Percent_Identity=32.3795180722892, Blast_Score=264, Evalue=2e-71,
Organism=Escherichia coli, GI48994965, Length=653, Percent_Identity=28.7901990811639, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6322369, Length=486, Percent_Identity=28.1893004115226, Blast_Score=134, Evalue=4e-32,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82254; Mature: 82254

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVACCMDIQYASRSLSYPPIVLSVADSPSSSSPASPDSSASAAAADWLAKLNDAQREAVE
CEEEEEEHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YGADRVAHPSGALLVIAGAGSGKTNTLAHRVANLVVKGVDPRRILLLTFSRRAALEMTRR
HCHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHH
VTRIAGMALGTRGALAQGLTWSGTFHSVGARLLREYADLIGLSPSFTINDREDSADLLNL
HHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHH
VRHELGFSAKEKRFPSKSACFAIYSRVVNTGASLADVLNSAFPWCREWEADLRMLFAAYV
HHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DAKQKQSVLDYDDLLLYWSHMAAEPAIAADLSGRFDHVLVDEYQDTNRLQASILLALKPD
HHHHHHHHCCHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCCEEEEEEEEECCC
GRGLTVVGDDAQSIYSFRGATVRNILDFPAHFDPPARQVTLERNYRSTQPILEASNAVIG
CCEEEEECCCHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHCCCCEEE
LASERYTKNLWTDKPSAQRPHLVTVADDADQARYVVEQVLAAREAGMKLKSQAVLFRAAH
ECHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEECC
HSATLEIELTRRNIPFVKFGGLKFLDSVHVKDVLSVLRWAENPRDRVAGFRVVQLLPGVG
CCEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PATAARVLDDIAARADALGSAGGLEAAHGVSTCAAGALTAFAPPARTLEDWHPFVAMMAS
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VCGRQTPWPAEFEMVRRWYEPHLQRNHEDASIRQADLVQMESIAGTYASRERFLTELTLD
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
PPDATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNGVDGCIPSDLGTGSEEEIDEE
CCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
RRLLYVAMTRAKEDLHIVMPQRFYVHNQTHLGDRHVWASRTRFIPPHLLPLFDSHAWPPA
HHEEEEEECCCCCCEEEECCCEEEEECCCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCCC
PVASAPTEMGLAAAAQAKIEIAAKLRKMWD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVACCMDIQYASRSLSYPPIVLSVADSPSSSSPASPDSSASAAAADWLAKLNDAQREAVE
CEEEEEEHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YGADRVAHPSGALLVIAGAGSGKTNTLAHRVANLVVKGVDPRRILLLTFSRRAALEMTRR
HCHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHH
VTRIAGMALGTRGALAQGLTWSGTFHSVGARLLREYADLIGLSPSFTINDREDSADLLNL
HHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHH
VRHELGFSAKEKRFPSKSACFAIYSRVVNTGASLADVLNSAFPWCREWEADLRMLFAAYV
HHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DAKQKQSVLDYDDLLLYWSHMAAEPAIAADLSGRFDHVLVDEYQDTNRLQASILLALKPD
HHHHHHHHCCHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCCEEEEEEEEECCC
GRGLTVVGDDAQSIYSFRGATVRNILDFPAHFDPPARQVTLERNYRSTQPILEASNAVIG
CCEEEEECCCHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHCCCCEEE
LASERYTKNLWTDKPSAQRPHLVTVADDADQARYVVEQVLAAREAGMKLKSQAVLFRAAH
ECHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEECC
HSATLEIELTRRNIPFVKFGGLKFLDSVHVKDVLSVLRWAENPRDRVAGFRVVQLLPGVG
CCEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
PATAARVLDDIAARADALGSAGGLEAAHGVSTCAAGALTAFAPPARTLEDWHPFVAMMAS
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VCGRQTPWPAEFEMVRRWYEPHLQRNHEDASIRQADLVQMESIAGTYASRERFLTELTLD
HHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
PPDATSDESGVPLIDEDYLILSTIHSAKGQEWRNVFVLNGVDGCIPSDLGTGSEEEIDEE
CCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHH
RRLLYVAMTRAKEDLHIVMPQRFYVHNQTHLGDRHVWASRTRFIPPHLLPLFDSHAWPPA
HHEEEEEECCCCCCEEEECCCEEEEECCCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCCC
PVASAPTEMGLAAAAQAKIEIAAKLRKMWD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]