Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 186475553

GI number: 186475553

Start: 884570

End: 885541

Strand: Direct

Name: thyA [H]

Synonym: Bphy_0788

Alternate gene names: 186475553

Gene position: 884570-885541 (Clockwise)

Preceding gene: 186475552

Following gene: 186475556

Centisome position: 25.42

GC content: 63.07

Gene sequence:

>972_bases
ATGAAACAGTACCTCGACCTCGTCCGCACGATCCTCGATACCGGCAGCTGGCAGGAAAACCGCACCGGCATTCGCACGAT
CAGCATGCCCGGGGCGATGTTGCGTTTCGACCTTCAGCAGGGTTTCCCCGCCGTGACGACGAAGAAGCTCGCGTTCAAGT
CGGCGATCGGCGAACTGATCGGTTTTCTGCGTGCGTCCCGGAGCGCCGCCGATTTCCGAGCGCTCGGCTGCAAGGTCTGG
GACGCGAACGCGAACGAAAACGCGCAGTGGCTCGCCAACCCGTATCGCGAGGGTCCGGACGATCTCGGCGACGTGTACGG
CGTGCAGTGGCGGCGGTGGCCCGCGTACAAGGTGCTCGACGCGACGGCGAGCGCGCAGCTGGAAGACGCGGCCTCGCGCG
GCTATGCGCCCGTCACGGAATTCGAAGAGGACGGCATGCGCAAGGTGCTGCTGTACAAGGCGATCGACCAGCTTCGTCAG
TGCCTCGACGCGATCATGCACAACCCGTCCGACCGGCGGATCTTGTTTCATGCGTGGAATCCGGCCGTGCTCGAGGAAAT
CGCACTGCCTGCATGCCATCTGCTGTACCAGTTCCTGCCGAATGCATCGAAGCGCGAGATTTCTCTCTGCCTGTACATCC
GCAGCAACGACGTCGGACTCGGCACGCCGTTCAACCTGACGGAAGGGGCCGCGCTGCTGCATCTCGTCGGCCGGCTGACG
GGCTATACGCCGCGCTGGTTCACGTATTTCATCGGCGATGCGCATATCTATGAGAACCAGCTCGACATGTTGCAGCAGCA
ACTGACGCGCGATCCTTACGAGAGCCCGCAGTTTGCGATTTCGGAGCGCGTGCCCGACTACACGAAGACGGGTGTCTACG
AGCCCGAGTGGCTGGAGAAGGTGGAGCCGTCCGATTTCTCGCTGGTCGGCTACCGACATCACGAGCCGCTGACGGCGCCG
ATGGCGATCTGA

Upstream 100 bases:

>100_bases
GTCCGTTCGGCGCGCTCACCTGCGTTCGCCCGGCTTGCTAGAATCGGGGTTTTCCCAAGAACGGTCCGCTGCGCCCGCGC
GCCACGCCGAAGGAACCCGT

Downstream 100 bases:

>100_bases
GCGCAACGGCTGATTGTCCCCGATGTGAAAACGCCCACTGCCATTCGCGGTGGGCGTTTTCATTCGCGGGCGCCATGCGG
CGCGGGTTTAGCCGCGATGA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKQYLDLVRTILDTGSWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELIGFLRASRSAADFRALGCKVW
DANANENAQWLANPYREGPDDLGDVYGVQWRRWPAYKVLDATASAQLEDAASRGYAPVTEFEEDGMRKVLLYKAIDQLRQ
CLDAIMHNPSDRRILFHAWNPAVLEEIALPACHLLYQFLPNASKREISLCLYIRSNDVGLGTPFNLTEGAALLHLVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQQQLTRDPYESPQFAISERVPDYTKTGVYEPEWLEKVEPSDFSLVGYRHHEPLTAP
MAI

Sequences:

>Translated_323_residues
MKQYLDLVRTILDTGSWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELIGFLRASRSAADFRALGCKVW
DANANENAQWLANPYREGPDDLGDVYGVQWRRWPAYKVLDATASAQLEDAASRGYAPVTEFEEDGMRKVLLYKAIDQLRQ
CLDAIMHNPSDRRILFHAWNPAVLEEIALPACHLLYQFLPNASKREISLCLYIRSNDVGLGTPFNLTEGAALLHLVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQQQLTRDPYESPQFAISERVPDYTKTGVYEPEWLEKVEPSDFSLVGYRHHEPLTAP
MAI
>Mature_323_residues
MKQYLDLVRTILDTGSWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELIGFLRASRSAADFRALGCKVW
DANANENAQWLANPYREGPDDLGDVYGVQWRRWPAYKVLDATASAQLEDAASRGYAPVTEFEEDGMRKVLLYKAIDQLRQ
CLDAIMHNPSDRRILFHAWNPAVLEEIALPACHLLYQFLPNASKREISLCLYIRSNDVGLGTPFNLTEGAALLHLVGRLT
GYTPRWFTYFIGDAHIYENQLDMLQQQLTRDPYESPQFAISERVPDYTKTGVYEPEWLEKVEPSDFSLVGYRHHEPLTAP
MAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=323, Percent_Identity=34.984520123839, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1789191, Length=323, Percent_Identity=33.4365325077399, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI71993377, Length=284, Percent_Identity=33.8028169014084, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6324648, Length=274, Percent_Identity=30.6569343065693, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17137556, Length=322, Percent_Identity=34.7826086956522, Blast_Score=196, Evalue=1e-50,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36724; Mature: 36724

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLVRTILDTGSWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELI
CHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHEEEEHHCCCCCHHHHHHHHHHHHHHHH
GFLRASRSAADFRALGCKVWDANANENAQWLANPYREGPDDLGDVYGVQWRRWPAYKVLD
HHHHHCCCHHHHHHHCCEEEECCCCCCHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHH
ATASAQLEDAASRGYAPVTEFEEDGMRKVLLYKAIDQLRQCLDAIMHNPSDRRILFHAWN
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PAVLEEIALPACHLLYQFLPNASKREISLCLYIRSNDVGLGTPFNLTEGAALLHLVGRLT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GYTPRWFTYFIGDAHIYENQLDMLQQQLTRDPYESPQFAISERVPDYTKTGVYEPEWLEK
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHCCCCCHHHHHH
VEPSDFSLVGYRHHEPLTAPMAI
CCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLVRTILDTGSWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELI
CHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHEEEEHHCCCCCHHHHHHHHHHHHHHHH
GFLRASRSAADFRALGCKVWDANANENAQWLANPYREGPDDLGDVYGVQWRRWPAYKVLD
HHHHHCCCHHHHHHHCCEEEECCCCCCHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHH
ATASAQLEDAASRGYAPVTEFEEDGMRKVLLYKAIDQLRQCLDAIMHNPSDRRILFHAWN
HHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
PAVLEEIALPACHLLYQFLPNASKREISLCLYIRSNDVGLGTPFNLTEGAALLHLVGRLT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
GYTPRWFTYFIGDAHIYENQLDMLQQQLTRDPYESPQFAISERVPDYTKTGVYEPEWLEK
CCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHCCCCCHHHHHH
VEPSDFSLVGYRHHEPLTAPMAI
CCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]