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The map label for this gene is trmD

Identifier: 186475536

GI number: 186475536

Start: 867370

End: 868137

Strand: Direct

Name: trmD

Synonym: Bphy_0771

Alternate gene names: 186475536

Gene position: 867370-868137 (Clockwise)

Preceding gene: 186475535

Following gene: 186475537

Centisome position: 24.93

GC content: 62.24

Gene sequence:

>768_bases
ATGCAGTTCGATATCGTTACGCTCTTTCCTGACATGTTTCGCGCGCTGACCGACTGGGGCATCACGAGCCGCGCAGCGAA
GCAGGAGCGTTATGGTTTGCGCACGTGGAATCCCCGCGATTTCACAACCGACAACTACCGCACAATCGACGATCGCCCGT
ACGGCGGCGGACCCGGCATGGTCATGCTGGCCAGGCCGCTGGAAGACGCGATCAACGCCGCGAAAGCGGCCCAGGCAGAG
CAGGGCATTGGCGGCGCACGCGTCGTGATGATGTCGCCGCAAGGCGCCACGCTCAATCACGACAAGGTCATGCGCTTTGC
CGCCGAGCCCGGTCTGATCCTGCTGTGCGGCCGCTATGAGGCGATCGATCAGCGTCTGATCGATCGTGTCGTCGACGAAG
AAGTTAGCCTCGGCGACTTCGTGCTGTCGGGCGGCGAGTTGCCGGCAATGGCATTGATTGATGCCGTCGTGCGACATCTG
CCGGGCGTGCTGAACGATGCGCAATCGGCGGTGCAGGACAGTTTCGTCGATGGCTTGCTGGATTGCCCGCACTACACGCG
TCCGGAAGAATACGACGGCGTGCGTGTGCCCGATGTGCTGCTCGGCGGCCATCATGCGGAAATCGAGCAATGGCGGCGGC
GCGAGGCTCTGCGCAATACGTGGTTGAAGCGGCCCGACTTGATCGTGCAGGCCAGAAAGAACAAGTTGTTGAGTCGCGCC
GACGAGGCGTGGCTCGCAAGTCTCGCGAAGGACGCATCGAAGCATTGA

Upstream 100 bases:

>100_bases
GTTCGTCGGCGTCTATGTCAAAACGGTGGATCAGGCGGCGAAGAAGATCATCGTCGACTGGGAAGCCGATTACTAGAACA
TTCGCTTCACGGAGAGAGCG

Downstream 100 bases:

>100_bases
GCTTCGGGCGACGACGCGGGATCGAGGCGGAGCCGTCCATGCGTGGCCGGTTCCAGATGTGAACCCATCCTCTATCGGGG
CCGTAGCCGGGCGCGAAGCG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MQFDIVTLFPDMFRALTDWGITSRAAKQERYGLRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLARPLEDAINAAKAAQAE
QGIGGARVVMMSPQGATLNHDKVMRFAAEPGLILLCGRYEAIDQRLIDRVVDEEVSLGDFVLSGGELPAMALIDAVVRHL
PGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYDGVRVPDVLLGGHHAEIEQWRRREALRNTWLKRPDLIVQARKNKLLSRA
DEAWLASLAKDASKH

Sequences:

>Translated_255_residues
MQFDIVTLFPDMFRALTDWGITSRAAKQERYGLRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLARPLEDAINAAKAAQAE
QGIGGARVVMMSPQGATLNHDKVMRFAAEPGLILLCGRYEAIDQRLIDRVVDEEVSLGDFVLSGGELPAMALIDAVVRHL
PGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYDGVRVPDVLLGGHHAEIEQWRRREALRNTWLKRPDLIVQARKNKLLSRA
DEAWLASLAKDASKH
>Mature_255_residues
MQFDIVTLFPDMFRALTDWGITSRAAKQERYGLRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLARPLEDAINAAKAAQAE
QGIGGARVVMMSPQGATLNHDKVMRFAAEPGLILLCGRYEAIDQRLIDRVVDEEVSLGDFVLSGGELPAMALIDAVVRHL
PGVLNDAQSAVQDSFVDGLLDCPHYTRPEEYDGVRVPDVLLGGHHAEIEQWRRREALRNTWLKRPDLIVQARKNKLLSRA
DEAWLASLAKDASKH

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=255, Percent_Identity=52.9411764705882, Blast_Score=262, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BURP8 (B2JF31)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001857006.1
- ProteinModelPortal:   B2JF31
- SMR:   B2JF31
- GeneID:   6242269
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0771
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28463; Mature: 28463

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: BINDING 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDIVTLFPDMFRALTDWGITSRAAKQERYGLRTWNPRDFTTDNYRTIDDRPYGGGPGM
CCCEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCE
VMLARPLEDAINAAKAAQAEQGIGGARVVMMSPQGATLNHDKVMRFAAEPGLILLCGRYE
EEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCHH
AIDQRLIDRVVDEEVSLGDFVLSGGELPAMALIDAVVRHLPGVLNDAQSAVQDSFVDGLL
HHHHHHHHHHHHCCCCCCHHEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
DCPHYTRPEEYDGVRVPDVLLGGHHAEIEQWRRREALRNTWLKRPDLIVQARKNKLLSRA
CCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DEAWLASLAKDASKH
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFDIVTLFPDMFRALTDWGITSRAAKQERYGLRTWNPRDFTTDNYRTIDDRPYGGGPGM
CCCEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCE
VMLARPLEDAINAAKAAQAEQGIGGARVVMMSPQGATLNHDKVMRFAAEPGLILLCGRYE
EEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCHH
AIDQRLIDRVVDEEVSLGDFVLSGGELPAMALIDAVVRHLPGVLNDAQSAVQDSFVDGLL
HHHHHHHHHHHHCCCCCCHHEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
DCPHYTRPEEYDGVRVPDVLLGGHHAEIEQWRRREALRNTWLKRPDLIVQARKNKLLSRA
CCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DEAWLASLAKDASKH
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA