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The map label for this gene is ispH [H]

Identifier: 186475354

GI number: 186475354

Start: 664699

End: 665658

Strand: Direct

Name: ispH [H]

Synonym: Bphy_0587

Alternate gene names: 186475354

Gene position: 664699-665658 (Clockwise)

Preceding gene: 186475353

Following gene: 186475355

Centisome position: 19.11

GC content: 61.88

Gene sequence:

>960_bases
ATGAGCATCACGGACACGACTCTCGCCGAAGCTGAAATCCTGCTTGCGCAGCCGCGCGGATTTTGCGCCGGCGTCGATCG
GGCGATCGAGATCGTCGAACGGGCCATCAAGCTGTACGGTTCGCCGATCTACGTACGTCACGAAATCGTTCATAACGCGT
ATGTCGTCGAAGACCTGCGCAAGAAGGGCGCGATCTTTATCGAGCAACTGGAAGAAGTGCCGTCGGGCAGCACGGTGATT
TTCAGTGCGCATGGCGTGTCGAAGGCCGTGCGCGCCGAAGCTGACGAGCGCGGCCTGCGCGTGTACGACGCGACGTGCCC
GCTCGTCACCAAGGTGCATATCGAAGTCGCGAAGATGCGCGCGGACGGCTTTGACATCGTGATGATCGGCCACAAGGGTC
ACCCTGAAGTGGAAGGCACGATGGGGCAGACGGCCGAGGGCATGTATCTCGTCGAAGATATCGACGACGTGCAGGCGTTG
CAGCTTGCAGATCCGGACCGCATCGCATACGTGACGCAAACCACGCTATCCGTCGACGACGCCGCGCAGATCATCGGGGC
GCTGAAGGTAAAATATCCGAACATCAAGGAGCCGAAGAAACAGGACATCTGCTACGCCACGCAAAACCGCCAGGACGCCG
TCAAGTTCATGGCGCCGCAGTGCGACGTGGTGATTGTCGTCGGCAGCCCGAACAGTTCCAATTCCAACCGCCTGCGCGAA
CTGGCCGAAAAGCTGGGTGTGCCTTCGTATATGGTGGACTCGCCAGACCAGATCGACCCCGCATGGGTCGCCGACAAGCG
ACGCATCGGCGTGACGGCGGGTGCATCGGCGCCCGAGGCGCTGGCTCAGGCCGTTATCGGCCGTCTGCGCGAACTCGGCG
TGCGCAACGTGCGCGCGCTCGAAGGGATCGAGGAAAACATCGCGTTTCCCTTGCCCCGCGGGTTGGGTTTGCCGGCCTGA

Upstream 100 bases:

>100_bases
TGAAGGAGGTTGGCGAAACCTCGGCTCTCTTCGATTTCAACCATCCGCTCGCCGGCCAGGCGCTGACGTTCGAAGTAAAA
ATCATCGGGATCCTGTAAAC

Downstream 100 bases:

>100_bases
CCTCCTGTCCCTATCAAAAAAAACGCGCCGCCGGCGCGTTTTTTTATTGCCCCGACACACCAAAAATCGCCAGAAAAAGG
TGCCACATCACATAAAAATT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MSITDTTLAEAEILLAQPRGFCAGVDRAIEIVERAIKLYGSPIYVRHEIVHNAYVVEDLRKKGAIFIEQLEEVPSGSTVI
FSAHGVSKAVRAEADERGLRVYDATCPLVTKVHIEVAKMRADGFDIVMIGHKGHPEVEGTMGQTAEGMYLVEDIDDVQAL
QLADPDRIAYVTQTTLSVDDAAQIIGALKVKYPNIKEPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNSNRLRE
LAEKLGVPSYMVDSPDQIDPAWVADKRRIGVTAGASAPEALAQAVIGRLRELGVRNVRALEGIEENIAFPLPRGLGLPA

Sequences:

>Translated_319_residues
MSITDTTLAEAEILLAQPRGFCAGVDRAIEIVERAIKLYGSPIYVRHEIVHNAYVVEDLRKKGAIFIEQLEEVPSGSTVI
FSAHGVSKAVRAEADERGLRVYDATCPLVTKVHIEVAKMRADGFDIVMIGHKGHPEVEGTMGQTAEGMYLVEDIDDVQAL
QLADPDRIAYVTQTTLSVDDAAQIIGALKVKYPNIKEPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNSNRLRE
LAEKLGVPSYMVDSPDQIDPAWVADKRRIGVTAGASAPEALAQAVIGRLRELGVRNVRALEGIEENIAFPLPRGLGLPA
>Mature_318_residues
SITDTTLAEAEILLAQPRGFCAGVDRAIEIVERAIKLYGSPIYVRHEIVHNAYVVEDLRKKGAIFIEQLEEVPSGSTVIF
SAHGVSKAVRAEADERGLRVYDATCPLVTKVHIEVAKMRADGFDIVMIGHKGHPEVEGTMGQTAEGMYLVEDIDDVQALQ
LADPDRIAYVTQTTLSVDDAAQIIGALKVKYPNIKEPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNSNRLREL
AEKLGVPSYMVDSPDQIDPAWVADKRRIGVTAGASAPEALAQAVIGRLRELGVRNVRALEGIEENIAFPLPRGLGLPA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=307, Percent_Identity=58.6319218241042, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 34680; Mature: 34549

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITDTTLAEAEILLAQPRGFCAGVDRAIEIVERAIKLYGSPIYVRHEIVHNAYVVEDLR
CCCCCCHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
KKGAIFIEQLEEVPSGSTVIFSAHGVSKAVRAEADERGLRVYDATCPLVTKVHIEVAKMR
HCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCCCEEEECCCCEEEHEEEEEEEEC
ADGFDIVMIGHKGHPEVEGTMGQTAEGMYLVEDIDDVQALQLADPDRIAYVTQTTLSVDD
CCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHEEECCCCCEEEEEEEEEECHHH
AAQIIGALKVKYPNIKEPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNSNRLRE
HHHHHHHHEECCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
LAEKLGVPSYMVDSPDQIDPAWVADKRRIGVTAGASAPEALAQAVIGRLRELGVRNVRAL
HHHHHCCCCEECCCCCCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHH
EGIEENIAFPLPRGLGLPA
HCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SITDTTLAEAEILLAQPRGFCAGVDRAIEIVERAIKLYGSPIYVRHEIVHNAYVVEDLR
CCCCCHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
KKGAIFIEQLEEVPSGSTVIFSAHGVSKAVRAEADERGLRVYDATCPLVTKVHIEVAKMR
HCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCCCCEEEECCCCEEEHEEEEEEEEC
ADGFDIVMIGHKGHPEVEGTMGQTAEGMYLVEDIDDVQALQLADPDRIAYVTQTTLSVDD
CCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHEEECCCCCEEEEEEEEEECHHH
AAQIIGALKVKYPNIKEPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNSNRLRE
HHHHHHHHEECCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
LAEKLGVPSYMVDSPDQIDPAWVADKRRIGVTAGASAPEALAQAVIGRLRELGVRNVRAL
HHHHHCCCCEECCCCCCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHH
EGIEENIAFPLPRGLGLPA
HCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA