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The map label for this gene is rbsB [H]

Identifier: 186475268

GI number: 186475268

Start: 571056

End: 572021

Strand: Direct

Name: rbsB [H]

Synonym: Bphy_0500

Alternate gene names: 186475268

Gene position: 571056-572021 (Clockwise)

Preceding gene: 186475267

Following gene: 186475269

Centisome position: 16.41

GC content: 63.25

Gene sequence:

>966_bases
ATGACGCATAGCAACGCATCAAATGGCACATCGCAACAGCCCCGCACGCTGCGCCTTCGCGCGACGCTCGCCTTCGCCGC
CGTGATGCTCGGCACACCGCTGTTGGCGCCCGCCGCCGCGGACGCCGCGCCGCTGAAGATCGGCATGACGTTCCAGGAGC
TGAACAATCCTTACTTCGTAACGATGCAAAAGGCGCTCAACGATGCCGCGGCGTCGATCGGCGCGACGGTCGTCGTCACC
GACGCGCACCACGACGTGAGCAAGCAGGTCAGCGACGTCGAAGACATGCTGCAGAAGAAGATCGACATTCTGCTGGTGAA
TCCGACCGACTCGACGGGTATCCAGTCGGCCGTCACGTCTGCGAAGAAGGCAGGCGCCGTGGTCGTCGCGGTCGACGCGA
ACGCGAACGGCCCTGTCGATTCGTTTGTCGGCTCGAAGAATTTCGACGCCGGCCAGATGTCGTGCGAATACCTCGCGAAG
GCGATCGGCGGCAGCGGCGAGGTGGCAATTCTCGATGGCATCCCCGTCGTGCCGATTCTCGAGCGGGTGCGCGGGTGCAA
GGCGGCGCTCGCGAAGTTCCCGAATGTGAAGGTCGTCGACACGCAGAACGGCAAGCAGGAGCGCGCAACGGCGCTGTCCG
TCACGGAGAACATGATCCAGGCGCATCCGAACCTGAAGGGCATCTTCAGTGTGAACGACGGCGGCTCGATGGGTGCCTTG
TCCGCCATCGAATCGTCGGGCAAGGACATCAAGCTGACGAGCGTCGACGGCGCGCCGGAAGCGATCACCGCAATCCAGAA
GCCGAACTCGAAGTTCATCGAAACGTCCGCGCAGTTCCCACGCGACCAGATTCGCCTCGCGATCGGCGTGGCGCTCGCGA
AGAAGTGGGGCGCCAATGTGCCGAAGACGATTCCCGTCGACGTGAAACTGGTCGACAAGGACAACGCGAAGGGCTTCAGC
TGGTAA

Upstream 100 bases:

>100_bases
CCGACGAACTAGGGCACCCCAGCAATCCCGACGTTTCACCTCCTCATCGCGCTTCAGACAGCGCGAGGGAAGCACTCATC
CTCATAAACGGAGACAAACC

Downstream 100 bases:

>100_bases
GCAAGGCGTCGTGTATGCGGTGACGGGTGCAGGCACGCGGCACCGCATGAACAAGGAAACGCGATGGACACGATCCTCAA
ACTCGACAACATCACGAAGA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTHSNASNGTSQQPRTLRLRATLAFAAVMLGTPLLAPAAADAAPLKIGMTFQELNNPYFVTMQKALNDAAASIGATVVVT
DAHHDVSKQVSDVEDMLQKKIDILLVNPTDSTGIQSAVTSAKKAGAVVVAVDANANGPVDSFVGSKNFDAGQMSCEYLAK
AIGGSGEVAILDGIPVVPILERVRGCKAALAKFPNVKVVDTQNGKQERATALSVTENMIQAHPNLKGIFSVNDGGSMGAL
SAIESSGKDIKLTSVDGAPEAITAIQKPNSKFIETSAQFPRDQIRLAIGVALAKKWGANVPKTIPVDVKLVDKDNAKGFS
W

Sequences:

>Translated_321_residues
MTHSNASNGTSQQPRTLRLRATLAFAAVMLGTPLLAPAAADAAPLKIGMTFQELNNPYFVTMQKALNDAAASIGATVVVT
DAHHDVSKQVSDVEDMLQKKIDILLVNPTDSTGIQSAVTSAKKAGAVVVAVDANANGPVDSFVGSKNFDAGQMSCEYLAK
AIGGSGEVAILDGIPVVPILERVRGCKAALAKFPNVKVVDTQNGKQERATALSVTENMIQAHPNLKGIFSVNDGGSMGAL
SAIESSGKDIKLTSVDGAPEAITAIQKPNSKFIETSAQFPRDQIRLAIGVALAKKWGANVPKTIPVDVKLVDKDNAKGFS
W
>Mature_320_residues
THSNASNGTSQQPRTLRLRATLAFAAVMLGTPLLAPAAADAAPLKIGMTFQELNNPYFVTMQKALNDAAASIGATVVVTD
AHHDVSKQVSDVEDMLQKKIDILLVNPTDSTGIQSAVTSAKKAGAVVVAVDANANGPVDSFVGSKNFDAGQMSCEYLAKA
IGGSGEVAILDGIPVVPILERVRGCKAALAKFPNVKVVDTQNGKQERATALSVTENMIQAHPNLKGIFSVNDGGSMGALS
AIESSGKDIKLTSVDGAPEAITAIQKPNSKFIETSAQFPRDQIRLAIGVALAKKWGANVPKTIPVDVKLVDKDNAKGFSW

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=274, Percent_Identity=35.036496350365, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790526, Length=305, Percent_Identity=29.8360655737705, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790674, Length=205, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788898, Length=247, Percent_Identity=23.0769230769231, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789990, Length=221, Percent_Identity=28.0542986425339, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33517; Mature: 33385

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSNASNGTSQQPRTLRLRATLAFAAVMLGTPLLAPAAADAAPLKIGMTFQELNNPYFV
CCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHCCCCEEE
TMQKALNDAAASIGATVVVTDAHHDVSKQVSDVEDMLQKKIDILLVNPTDSTGIQSAVTS
EEHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
AKKAGAVVVAVDANANGPVDSFVGSKNFDAGQMSCEYLAKAIGGSGEVAILDGIPVVPIL
HHHCCEEEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHH
ERVRGCKAALAKFPNVKVVDTQNGKQERATALSVTENMIQAHPNLKGIFSVNDGGSMGAL
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
SAIESSGKDIKLTSVDGAPEAITAIQKPNSKFIETSAQFPRDQIRLAIGVALAKKWGANV
HHHHCCCCEEEEEECCCCCHHHHHHCCCCCHHHHCHHHCCHHHHHHHHHHHHHHHHCCCC
PKTIPVDVKLVDKDNAKGFSW
CCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
THSNASNGTSQQPRTLRLRATLAFAAVMLGTPLLAPAAADAAPLKIGMTFQELNNPYFV
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHCCCCEEE
TMQKALNDAAASIGATVVVTDAHHDVSKQVSDVEDMLQKKIDILLVNPTDSTGIQSAVTS
EEHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHH
AKKAGAVVVAVDANANGPVDSFVGSKNFDAGQMSCEYLAKAIGGSGEVAILDGIPVVPIL
HHHCCEEEEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHH
ERVRGCKAALAKFPNVKVVDTQNGKQERATALSVTENMIQAHPNLKGIFSVNDGGSMGAL
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
SAIESSGKDIKLTSVDGAPEAITAIQKPNSKFIETSAQFPRDQIRLAIGVALAKKWGANV
HHHHCCCCEEEEEECCCCCHHHHHHCCCCCHHHHCHHHCCHHHHHHHHHHHHHHHHCCCC
PKTIPVDVKLVDKDNAKGFSW
CCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]