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The map label for this gene is frmB [H]

Identifier: 186475252

GI number: 186475252

Start: 552681

End: 553526

Strand: Direct

Name: frmB [H]

Synonym: Bphy_0484

Alternate gene names: 186475252

Gene position: 552681-553526 (Clockwise)

Preceding gene: 186475251

Following gene: 186475257

Centisome position: 15.89

GC content: 64.3

Gene sequence:

>846_bases
ATGCTCGAACTGATCGAATCGCACGCGTCGTTCGGCGGCACGCAGCGTATCTACAGCCACGAGTCGAAGGCGATCGGCTT
GCCGATGCGCTTTTCCGTCTTCATGCCGAAAGAGGCGTCGCAGAAGAAGGTGCCGGCGCTGTTTTATCTGGCCGGGCTGA
CCTGCACCGAAGAGACGTTTCCGATCAAGGCGGGCGCGCAGCGCTTCGCCGCACAGCACGGCATCGCGCTGATCGCGCCC
GACACCAGTCCGCGCGGCGCAGGCGTGCCGGGCGAGACGGACTCGTGGGACTTCGGCGTCGGCGCGGGCTTCTACGTCGA
TGCGACGCAGGAACCCTGGTCGAAGCACTATCGGATGTATTCATACGTGCGTGACGAGCTGCGCGAGACGGTCGTCAACG
AATTGCCCGTCGACGGCGCGCGGCTTGGCATCTTTGGCCACTCGATGGGCGGTCATGGCGCGCTGATGCTGGCTTTGCGC
AATCCGGACATCTATCGGTCCGTGTCGGCGTTCGCGCCGATTGCCGCGCCGACGCGCTGTCCGTGGGGCGAGAAAGCGTT
CAGCGGTTACCTGGGCGCCGATCGCGAAGCGTGGAAGCACTACGACGCAAGCGAACTCGTCGGCAAGGCCTCACGCAAGT
TTGCCGAAGGGATTCTGGTGGATCAGGGGCTCGCCGACAACTTTCTCGCGCAGCAGCTGAACCCCGACGTGTTCGAAGCC
GCGTGCAAGGCAGCCGATCAGCCGCTCACGCTGCGCCGGCATGAGGGCTACGACCACGGCTACTTCTTCATTTCGACGTT
CATCGAGGACCACCTCGCCCATCACGCGAAGGTGCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
TCACACATCACCTGAAGCTCGATCAGATCAACGAAGGCTTCGACCTGATGAAGAAGGGCGAGTCGATCCGCTCCGTCGTC
ATCTACTAAAGGAGCGCGCG

Downstream 100 bases:

>100_bases
AGTTGCGGGCCGCAAGTGTAGAAAGCAAAACGGGGTGGCTCCTTTAGCCACCCCGTTTCTTTTCGCGCTATCTGCGCGCA
TGTTGCACCAGCAGACTCGC

Product: S-formylglutathione hydrolase

Products: NA

Alternate protein names: FGH [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLELIESHASFGGTQRIYSHESKAIGLPMRFSVFMPKEASQKKVPALFYLAGLTCTEETFPIKAGAQRFAAQHGIALIAP
DTSPRGAGVPGETDSWDFGVGAGFYVDATQEPWSKHYRMYSYVRDELRETVVNELPVDGARLGIFGHSMGGHGALMLALR
NPDIYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKHYDASELVGKASRKFAEGILVDQGLADNFLAQQLNPDVFEA
ACKAADQPLTLRRHEGYDHGYFFISTFIEDHLAHHAKVLLG

Sequences:

>Translated_281_residues
MLELIESHASFGGTQRIYSHESKAIGLPMRFSVFMPKEASQKKVPALFYLAGLTCTEETFPIKAGAQRFAAQHGIALIAP
DTSPRGAGVPGETDSWDFGVGAGFYVDATQEPWSKHYRMYSYVRDELRETVVNELPVDGARLGIFGHSMGGHGALMLALR
NPDIYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKHYDASELVGKASRKFAEGILVDQGLADNFLAQQLNPDVFEA
ACKAADQPLTLRRHEGYDHGYFFISTFIEDHLAHHAKVLLG
>Mature_281_residues
MLELIESHASFGGTQRIYSHESKAIGLPMRFSVFMPKEASQKKVPALFYLAGLTCTEETFPIKAGAQRFAAQHGIALIAP
DTSPRGAGVPGETDSWDFGVGAGFYVDATQEPWSKHYRMYSYVRDELRETVVNELPVDGARLGIFGHSMGGHGALMLALR
NPDIYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKHYDASELVGKASRKFAEGILVDQGLADNFLAQQLNPDVFEA
ACKAADQPLTLRRHEGYDHGYFFISTFIEDHLAHHAKVLLG

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate [H]

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

Organism=Homo sapiens, GI33413400, Length=279, Percent_Identity=55.1971326164875, Blast_Score=330, Evalue=9e-91,
Organism=Escherichia coli, GI1786551, Length=278, Percent_Identity=50, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1788477, Length=279, Percent_Identity=51.2544802867383, Blast_Score=288, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17510185, Length=278, Percent_Identity=51.0791366906475, Blast_Score=289, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6322393, Length=296, Percent_Identity=37.8378378378378, Blast_Score=211, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45551932, Length=281, Percent_Identity=52.6690391459075, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24648347, Length=281, Percent_Identity=52.6690391459075, Blast_Score=302, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801
- InterPro:   IPR014186 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: =3.1.2.12 [H]

Molecular weight: Translated: 30882; Mature: 30882

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELIESHASFGGTQRIYSHESKAIGLPMRFSVFMPKEASQKKVPALFYLAGLTCTEETF
CHHHHHHHHCCCCHHHHHHCCCCEECCCEEEEEECCCCCCCCCCCEEEEEECCEECCCCC
PIKAGAQRFAAQHGIALIAPDTSPRGAGVPGETDSWDFGVGAGFYVDATQEPWSKHYRMY
CCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHH
SYVRDELRETVVNELPVDGARLGIFGHSMGGHGALMLALRNPDIYRSVSAFAPIAAPTRC
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCC
PWGEKAFSGYLGADREAWKHYDASELVGKASRKFAEGILVDQGLADNFLAQQLNPDVFEA
CCCHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHH
ACKAADQPLTLRRHEGYDHGYFFISTFIEDHLAHHAKVLLG
HHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLELIESHASFGGTQRIYSHESKAIGLPMRFSVFMPKEASQKKVPALFYLAGLTCTEETF
CHHHHHHHHCCCCHHHHHHCCCCEECCCEEEEEECCCCCCCCCCCEEEEEECCEECCCCC
PIKAGAQRFAAQHGIALIAPDTSPRGAGVPGETDSWDFGVGAGFYVDATQEPWSKHYRMY
CCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHH
SYVRDELRETVVNELPVDGARLGIFGHSMGGHGALMLALRNPDIYRSVSAFAPIAAPTRC
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCC
PWGEKAFSGYLGADREAWKHYDASELVGKASRKFAEGILVDQGLADNFLAQQLNPDVFEA
CCCHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHH
ACKAADQPLTLRRHEGYDHGYFFISTFIEDHLAHHAKVLLG
HHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA