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The map label for this gene is flhA [H]

Identifier: 186475251

GI number: 186475251

Start: 551563

End: 552669

Strand: Direct

Name: flhA [H]

Synonym: Bphy_0483

Alternate gene names: 186475251

Gene position: 551563-552669 (Clockwise)

Preceding gene: 186475250

Following gene: 186475252

Centisome position: 15.85

GC content: 61.34

Gene sequence:

>1107_bases
ATGAAGACGAAAGCAGCAATCGCATGGAAAGCAGGCGCACCGTTGACGATCGAAGAAGTCGATCTCGAAGGTCCGCGCGC
CGGTGAGGTGCTGATCGAGGTGAAGGCGACGGGTATTTGCCACACCGACTACTACACGCTGTCGGGCGCCGATCCGGAAG
GGCTCTTCCCCGCGATTCTCGGTCATGAGGGCGCAGGCGTGATTGTCGATACCGGCCCCGGCGTCGGCACGCTGAAGAAG
GGCGATCACGTCATTCCGCTCTACACGCCCGAGTGCCGTCAATGCAAGTTCTGTTTGTCGCGCAAGACAAACCTGTGTCA
GGCGATCCGCGCGACGCAGGGCAAGGGCCTGATGCCCGATGCGACCTCGCGTTTCTCGCTCGACGGCAAGCCGCTGTTCC
ACTACATGGGCACGTCCACGTTCTCGAACTACATCGTCGTGCCGGAAATCGCGGTCGCGAAGATTCGTGAAGACGCGCCG
TTCGACAAGGTCTGCTACATCGGCTGCGGCGTGACGACGGGTGTCGGCGCGGTCGTGTATTCGGCGAAGGTGGAAGCGGG
TGCGAATGTCGTCGTGTTCGGTCTGGGTGGCATCGGCCTCAATGTGATCCAGGGCGCCAAAATGGTAGGGGCCGACAAGA
TCATCGGCGTCGATATCAACCCGGGCCGCGTCGAACTCGCGAAGAAGTTCGGCATGACGCACTTCATCAACCCGAACGAG
GTCGAGAACGTCGTCGATCACATCGTGCAACTGACTGACGGCGGCGCGGACTACTCGTTTGAATGCGTCGGCAACACGAC
GCTGATGCGCCAGGCGCTCGAGTGCACGCACAAGGGCTGGGGCCAGTCGTTCATCATCGGCGTGGCGGCGGCCGGTCAGG
AAATCAGCACGCGTCCGTTCCAGCTGGTCACGGGCCGCGAGTGGAAGGGCTCGGCGTTCGGCGGCGCGCGGGGCCGCACC
GATGTGCCGAAGATCGTCGACTGGTACATGGAAGGCAAGATCAATATCGACGACCTGATCACACATCACCTGAAGCTCGA
TCAGATCAACGAAGGCTTCGACCTGATGAAGAAGGGCGAGTCGATCCGCTCCGTCGTCATCTACTAA

Upstream 100 bases:

>100_bases
GCGTGAGCGACAATGCGGGCCGCAACGGTTCAAGGCAACCCGCACGCGGATTCGCAAGAACGGCGGGTAACAGGTTCAAG
GAACTACAGGAGCATTCACG

Downstream 100 bases:

>100_bases
AGGAGCGCGCGATGCTCGAACTGATCGAATCGCACGCGTCGTTCGGCGGCACGCAGCGTATCTACAGCCACGAGTCGAAG
GCGATCGGCTTGCCGATGCG

Product: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVIVDTGPGVGTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKIREDAP
FDKVCYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPGRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNTTLMRQALECTHKGWGQSFIIGVAAAGQEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHHLKLDQINEGFDLMKKGESIRSVVIY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVIVDTGPGVGTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKIREDAP
FDKVCYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPGRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNTTLMRQALECTHKGWGQSFIIGVAAAGQEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHHLKLDQINEGFDLMKKGESIRSVVIY
>Mature_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAILGHEGAGVIVDTGPGVGTLKK
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKIREDAP
FDKVCYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPGRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNTTLMRQALECTHKGWGQSFIIGVAAAGQEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHHLKLDQINEGFDLMKKGESIRSVVIY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=60.2179836512262, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.5935828877005, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=45.7994579945799, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.5284552845528, Blast_Score=339, Evalue=2e-93,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.2574525745258, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=43.75, Blast_Score=329, Evalue=2e-90,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=43.7837837837838, Blast_Score=327, Evalue=9e-90,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=43.7837837837838, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=44.0443213296399, Blast_Score=323, Evalue=2e-88,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=58.5365853658537, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI87082125, Length=379, Percent_Identity=26.9129287598945, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790045, Length=376, Percent_Identity=27.1276595744681, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=26.0387811634349, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786825, Length=373, Percent_Identity=26.0053619302949, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787863, Length=349, Percent_Identity=26.0744985673352, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI226510992, Length=372, Percent_Identity=24.4623655913978, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081918, Length=316, Percent_Identity=28.4810126582279, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788075, Length=376, Percent_Identity=28.4574468085106, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790718, Length=326, Percent_Identity=24.2331288343558, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786518, Length=374, Percent_Identity=24.8663101604278, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.4235924932976, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.1333333333333, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17565904, Length=337, Percent_Identity=55.1928783382789, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17562876, Length=355, Percent_Identity=27.887323943662, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=26.7029972752044, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562582, Length=363, Percent_Identity=24.7933884297521, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562878, Length=362, Percent_Identity=25.1381215469613, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=53.3692722371968, Blast_Score=425, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6324486, Length=371, Percent_Identity=26.9541778975741, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=371, Percent_Identity=28.0323450134771, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=372, Percent_Identity=27.9569892473118, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=371, Percent_Identity=28.3018867924528, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=26.6483516483516, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=365, Percent_Identity=28.4931506849315, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=365, Percent_Identity=28.4931506849315, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=369, Percent_Identity=25.4742547425474, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=60.3260869565217, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17137530, Length=341, Percent_Identity=28.1524926686217, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17737897, Length=346, Percent_Identity=26.5895953757225, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39464; Mature: 39464

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGVIVDTGPGVGTLKKGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKIREDAPFDKVCYIGCGVTTGVGAVVY
CCCEEECCCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPGRVELAKKFGMTHFINPNE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEEECCCCHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECVGNTTLMRQALECTHKGWGQSFIIGVAAAGQEISTRPF
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHHLKLDQINEGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHEECCCCCCCHHHHHCCC
SIRSVVIY
CCEEEEEC
>Mature Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGVIVDTGPGVGTLKKGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKIREDAPFDKVCYIGCGVTTGVGAVVY
CCCEEECCCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPGRVELAKKFGMTHFINPNE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEEECCCCHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECVGNTTLMRQALECTHKGWGQSFIIGVAAAGQEISTRPF
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHHLKLDQINEGFDLMKKGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHEECCCCCCCHHHHHCCC
SIRSVVIY
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]