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The map label for this gene is pykA [H]

Identifier: 186475228

GI number: 186475228

Start: 522131

End: 523567

Strand: Direct

Name: pykA [H]

Synonym: Bphy_0460

Alternate gene names: 186475228

Gene position: 522131-523567 (Clockwise)

Preceding gene: 186475227

Following gene: 186475229

Centisome position: 15.01

GC content: 64.65

Gene sequence:

>1437_bases
ATGCATCGCGCCACCAAGATTGTCGCAACCATCGGACCCGCTTCCAGCACGCAGGACATCCTGCTGCAGATGATTCAGGC
GGGCGCCGACGTGGTGCGTCTCAACTTCTCGCACGGCACCGCCGACGACCATCGTCAACGCGCGGAGTTCGTACGTGAAG
CGGCCCGGCAGGCAGGCCGCGAGGTCGGCATCATGGCCGATCTGCAAGGCCCGAAAATCCGTGTCGGCAAGTTCGAAAAC
GGCAAGACCACGCTGATCGCCGGCAACACCTTCATCCTCGACGCCGACTGCGAACTCGGCAACGACGACCGCGTCGGCCT
CGATTACAAGGATCTGCCGCGCGACCTGCGAGCAGGCGACACGCTGCTTCTGAACGACGGCCTGATCGTGCTGACCGTGT
CCCGCGTGATTGGCAACGAAATCCACACCGTCGTGAAGATCGGCGGCGACCTGTCGAACAACAAGGGCATCAACCGCCAG
GGCGGCGGCCTGACGGCGCCGGCGCTGACCGCGAAGGACATGGAAGATATCCGCACGGCGATGTCGCTGGGTGCGGATTA
TGTGGCCGTGTCGTTCCCGAAGAATGCGACCGACATGGAGATGGCGCGCCAGCTCGCGAATATCGCGGGCGCGCCATACG
GCATCAAGCCGAAGATGATCGCGAAGATCGAGCGCGCCGAAGCAATTCCGGCGCTGCAAGGCATTCTCGAGGCATCGGAC
GGCATCATGGTCGCGCGTGGCGATCTCGCCGTCGAAGTCGGCAACGCGGCCGTGCCTGCGCTGCAAAAGCGCATGATCAA
GATGGCGCGCGACATGAACAAGTTCGTGATCACGGCCACGCAGATGATGGAGTCGATGATCCACGCGCCCGTGCCGACCC
GTGCGGAAGTGTCGGACGTCGCAAACGCGGTGCTGGACGGCACGGATGCAGTGATGCTGTCGGCGGAATCGGCGGCGGGC
AAGTACCCGGTGCAGACCATCGAGACGATGGCTGCCATCTGCGTCGAAGCCGAAAAGTCCGAGCACGTCGAGCTGGACAA
GGATTTCCTCGACCGCACGTTCACGCGCATCGACCAGTCGATCGCGATGGGCGCGCTCTTCACCGCGTATCACCTCGGTG
CGAAGGCGATCGTCGCGTTGACGGAATCGGGCTCGACGGCGCTGTGGATGTCGCGTCACTGGACGCACGTGCCCATCTTC
GCGCTCACGCCCCGCACGGGCAGCGAGCGCGCGATGGCGATCTACCGCAATGTCACTCCGCTGCATCTGGATACCAGCAG
CGATCGCGACATCGCGCTGGCGCAGGCGCTCGAAGTGGTGGTGGGCAAGGGCTATGCGGCGCGCGGCGACATGGTCGTGC
TCACGGTCGGCGAGCCGATGGGCCAGGCGGGGGGTACGAACACGCTGAAGATCGTGCGCGTCGGCGAACCCGTCTGA

Upstream 100 bases:

>100_bases
AACGAACGGCGCACCACCCCATGTAGCACCTGTCGTTGCGCAGCCTCCGCATCCGACTCCCAACGTTCATCCCAGCGATC
CCATCCAGACGAGGAGACTC

Downstream 100 bases:

>100_bases
CGCGAGCAGGCTGAGCCAGCCGAGCCAACTGTCAGCCGCCGCGAGAGCGAGTGGTAGCAAGCCGTTACGTTGAGTCAAGA
ACGGCACAGCCGAACGGCAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MHRATKIVATIGPASSTQDILLQMIQAGADVVRLNFSHGTADDHRQRAEFVREAARQAGREVGIMADLQGPKIRVGKFEN
GKTTLIAGNTFILDADCELGNDDRVGLDYKDLPRDLRAGDTLLLNDGLIVLTVSRVIGNEIHTVVKIGGDLSNNKGINRQ
GGGLTAPALTAKDMEDIRTAMSLGADYVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQGILEASD
GIMVARGDLAVEVGNAAVPALQKRMIKMARDMNKFVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVQTIETMAAICVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGSTALWMSRHWTHVPIF
ALTPRTGSERAMAIYRNVTPLHLDTSSDRDIALAQALEVVVGKGYAARGDMVVLTVGEPMGQAGGTNTLKIVRVGEPV

Sequences:

>Translated_478_residues
MHRATKIVATIGPASSTQDILLQMIQAGADVVRLNFSHGTADDHRQRAEFVREAARQAGREVGIMADLQGPKIRVGKFEN
GKTTLIAGNTFILDADCELGNDDRVGLDYKDLPRDLRAGDTLLLNDGLIVLTVSRVIGNEIHTVVKIGGDLSNNKGINRQ
GGGLTAPALTAKDMEDIRTAMSLGADYVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQGILEASD
GIMVARGDLAVEVGNAAVPALQKRMIKMARDMNKFVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVQTIETMAAICVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGSTALWMSRHWTHVPIF
ALTPRTGSERAMAIYRNVTPLHLDTSSDRDIALAQALEVVVGKGYAARGDMVVLTVGEPMGQAGGTNTLKIVRVGEPV
>Mature_478_residues
MHRATKIVATIGPASSTQDILLQMIQAGADVVRLNFSHGTADDHRQRAEFVREAARQAGREVGIMADLQGPKIRVGKFEN
GKTTLIAGNTFILDADCELGNDDRVGLDYKDLPRDLRAGDTLLLNDGLIVLTVSRVIGNEIHTVVKIGGDLSNNKGINRQ
GGGLTAPALTAKDMEDIRTAMSLGADYVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQGILEASD
GIMVARGDLAVEVGNAAVPALQKRMIKMARDMNKFVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAESAAG
KYPVQTIETMAAICVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGSTALWMSRHWTHVPIF
ALTPRTGSERAMAIYRNVTPLHLDTSSDRDIALAQALEVVVGKGYAARGDMVVLTVGEPMGQAGGTNTLKIVRVGEPV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=36.5111561866126, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=36.5111561866126, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=36.105476673428, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=36.8951612903226, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=36.8951612903226, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=36.0824742268041, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=36.0824742268041, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.0543933054393, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.0543933054393, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=31.651376146789, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=51.255230125523, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1787965, Length=468, Percent_Identity=39.5299145299145, Blast_Score=308, Evalue=7e-85,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=36.8098159509202, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=36.9609856262834, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=36.9609856262834, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=36.9609856262834, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=36.9609856262834, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=33.8809034907598, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=34.7648261758691, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24648964, Length=497, Percent_Identity=36.4185110663984, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI28571814, Length=497, Percent_Identity=36.4185110663984, Blast_Score=262, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=36.6748166259169, Blast_Score=231, Evalue=8e-61,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=30.879345603272, Blast_Score=212, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24646914, Length=270, Percent_Identity=36.2962962962963, Blast_Score=143, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51094; Mature: 51094

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRATKIVATIGPASSTQDILLQMIQAGADVVRLNFSHGTADDHRQRAEFVREAARQAGR
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
EVGIMADLQGPKIRVGKFENGKTTLIAGNTFILDADCELGNDDRVGLDYKDLPRDLRAGD
EECEEEECCCCEEEEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCCHHHCCHHHCCCC
TLLLNDGLIVLTVSRVIGNEIHTVVKIGGDLSNNKGINRQGGGLTAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADYVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQGILEASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GIMVARGDLAVEVGNAAVPALQKRMIKMARDMNKFVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAESAAGKYPVQTIETMAAICVEAEKSEHVELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIVALTESGSTALWMSRHWTHVPIFALTPRTGSERAMAIYRNVTP
HHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCE
LHLDTSSDRDIALAQALEVVVGKGYAARGDMVVLTVGEPMGQAGGTNTLKIVRVGEPV
EEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MHRATKIVATIGPASSTQDILLQMIQAGADVVRLNFSHGTADDHRQRAEFVREAARQAGR
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCC
EVGIMADLQGPKIRVGKFENGKTTLIAGNTFILDADCELGNDDRVGLDYKDLPRDLRAGD
EECEEEECCCCEEEEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCCHHHCCHHHCCCC
TLLLNDGLIVLTVSRVIGNEIHTVVKIGGDLSNNKGINRQGGGLTAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADYVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQGILEASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GIMVARGDLAVEVGNAAVPALQKRMIKMARDMNKFVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAESAAGKYPVQTIETMAAICVEAEKSEHVELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIVALTESGSTALWMSRHWTHVPIFALTPRTGSERAMAIYRNVTP
HHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEEECCCE
LHLDTSSDRDIALAQALEVVVGKGYAARGDMVVLTVGEPMGQAGGTNTLKIVRVGEPV
EEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]