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The map label for this gene is pgk [H]

Identifier: 186475227

GI number: 186475227

Start: 520452

End: 521645

Strand: Direct

Name: pgk [H]

Synonym: Bphy_0459

Alternate gene names: 186475227

Gene position: 520452-521645 (Clockwise)

Preceding gene: 186475226

Following gene: 186475228

Centisome position: 14.96

GC content: 64.74

Gene sequence:

>1194_bases
ATGAACAATGTATTGCGTCTCTCCGATCTGATCGCCGAAGGCAAGCTATCCGGCAAACGCGTCTTCATCCGCGCCGACCT
GAATGTGCCGCAGGACGACGCAGGCAACATCACCGAAGACACCCGCATACGCGCTTCCGTGCCCGCCATCAAGTCCGCGC
TGGACGCGGGCGCGGCCGTCATGGTCACGTCGCACCTGGGTCGACCGACGGAAGGCGACTTCAAGCCTGAAGATTCGCTC
GCGCCTGTCGCGAAGCGCCTCGCCGAACTGCTCGGCCGCGACGTACCGCTCGTGCAGAACTGGGTCGAGAACGGCGTGAA
CGTCGCGCCGGGCCAGGTGGTGCTGCTCGAAAACTGCCGCGTGAACAAGGGCGAAAAGAAGGATTCCGACGAGCTCGCGC
AAAAAATGGCGAAGCTCTGCGACGTGTACGTGAACGACGCGTTCGGCACCGCGCACCGCGCCGAAGCGACCACGCACGGC
ATTGCGAAGTACGCGAGCGTCGCATGCGCGGGTCCGCTTCTCGCGGCTGAACTGGAGGCGCTCGGCAAGGCGCTCGGCGC
GCCGAAGCGCCCGCTCGTGGCGATCGTCGCGGGTTCGAAGGTGTCGACCAAGCTGACCATTCTGAAGTCGCTCGCCGATA
AGGTCGATCAGTTGATCGTCGGCGGGGGGATCGCGAACACGTTCATGTTGGCGGCGGGCCTGAAGATCGGCAAGTCGCTG
GTCGAAGCGGATCTCGTCGATGAAGCGAAGGCGATCATCGAGGCAGCCAAGGCGCGCGGCGCATCCGTGCCGATCCCGTC
GGACGTCGTGACGGCCAAAGAATTCTCGGCAACGGCGCAAGCCACCGTCAAGCAGGTCGCCGACATCGAAGACGACGACA
TGATCCTCGACATCGGACCGGAAACGGCGAAAGCGCTCGCTGGCCAGCTCGAAAAGGCAGGCACGGTGGTCTGGAACGGC
CCCGTCGGCGTGTTCGAGTTCGACCAGTTCGGCAACGGCACGAAGACGCTCGCCGAAGCCATCGCCAGGTCGTCGGCGTA
CTCGATCGCAGGCGGCGGCGACACGCTGGCGGCCATCGCCAAATACGGCATCCACGACAAGGTGAGCTATATCTCGACGG
GCGGCGGCGCATTCCTCGAGTTCCTCGAGGGCAAGAAGCTGCCCGCAGTCGCCGTGCTGGAATCGCGGGCCTGA

Upstream 100 bases:

>100_bases
ATAGCCGTCTCCCGGAACATGCGCGAAGCCGCCGGCACCGTTCGCGAGGGGCCGTTCGCGCAGCGTCCGCACCGCCTTCC
AATCAACTCGCATACGAATC

Downstream 100 bases:

>100_bases
TAATGGCGACGCGCTCCCCCAACAAGCCTCAGAAGACGCGCAACGCGTCGCGCACGGCAGCGACAGGGGGGCGCGCAGCG
CGCTCCGCACAGGCGCTGCA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNNVLRLSDLIAEGKLSGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIKSALDAGAAVMVTSHLGRPTEGDFKPEDSL
APVAKRLAELLGRDVPLVQNWVENGVNVAPGQVVLLENCRVNKGEKKDSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYASVACAGPLLAAELEALGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
VEADLVDEAKAIIEAAKARGASVPIPSDVVTAKEFSATAQATVKQVADIEDDDMILDIGPETAKALAGQLEKAGTVVWNG
PVGVFEFDQFGNGTKTLAEAIARSSAYSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVAVLESRA

Sequences:

>Translated_397_residues
MNNVLRLSDLIAEGKLSGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIKSALDAGAAVMVTSHLGRPTEGDFKPEDSL
APVAKRLAELLGRDVPLVQNWVENGVNVAPGQVVLLENCRVNKGEKKDSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYASVACAGPLLAAELEALGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
VEADLVDEAKAIIEAAKARGASVPIPSDVVTAKEFSATAQATVKQVADIEDDDMILDIGPETAKALAGQLEKAGTVVWNG
PVGVFEFDQFGNGTKTLAEAIARSSAYSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVAVLESRA
>Mature_397_residues
MNNVLRLSDLIAEGKLSGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIKSALDAGAAVMVTSHLGRPTEGDFKPEDSL
APVAKRLAELLGRDVPLVQNWVENGVNVAPGQVVLLENCRVNKGEKKDSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYASVACAGPLLAAELEALGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
VEADLVDEAKAIIEAAKARGASVPIPSDVVTAKEFSATAQATVKQVADIEDDDMILDIGPETAKALAGQLEKAGTVVWNG
PVGVFEFDQFGNGTKTLAEAIARSSAYSIAGGGDTLAAIAKYGIHDKVSYISTGGGAFLEFLEGKKLPAVAVLESRA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=406, Percent_Identity=41.871921182266, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI4505763, Length=421, Percent_Identity=40.855106888361, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=63.0379746835443, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17508823, Length=404, Percent_Identity=44.8019801980198, Blast_Score=304, Evalue=5e-83,
Organism=Saccharomyces cerevisiae, GI6319857, Length=406, Percent_Identity=43.8423645320197, Blast_Score=287, Evalue=2e-78,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=41.9117647058824, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24581203, Length=405, Percent_Identity=39.0123456790123, Blast_Score=245, Evalue=4e-65,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41247; Mature: 41247

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVLRLSDLIAEGKLSGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIKSALDAGAAV
CCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCEE
MVTSHLGRPTEGDFKPEDSLAPVAKRLAELLGRDVPLVQNWVENGVNVAPGQVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEECCC
VNKGEKKDSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKYASVACAGPLLAAELEA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VEADLVDEAKAIIEAAKARGASVPIPSDVVTAKEFSATAQATVKQVADIEDDDMILDIGP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
ETAKALAGQLEKAGTVVWNGPVGVFEFDQFGNGTKTLAEAIARSSAYSIAGGGDTLAAIA
HHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHH
KYGIHDKVSYISTGGGAFLEFLEGKKLPAVAVLESRA
HHCCCCCHHHEECCCHHHHHHHCCCCCCEEEHCCCCC
>Mature Secondary Structure
MNNVLRLSDLIAEGKLSGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIKSALDAGAAV
CCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCEE
MVTSHLGRPTEGDFKPEDSLAPVAKRLAELLGRDVPLVQNWVENGVNVAPGQVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEECCC
VNKGEKKDSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKYASVACAGPLLAAELEA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGKALGAPKRPLVAIVAGSKVSTKLTILKSLADKVDQLIVGGGIANTFMLAAGLKIGKSL
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VEADLVDEAKAIIEAAKARGASVPIPSDVVTAKEFSATAQATVKQVADIEDDDMILDIGP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
ETAKALAGQLEKAGTVVWNGPVGVFEFDQFGNGTKTLAEAIARSSAYSIAGGGDTLAAIA
HHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCCEECCCCHHHHHHH
KYGIHDKVSYISTGGGAFLEFLEGKKLPAVAVLESRA
HHCCCCCHHHEECCCHHHHHHHCCCCCCEEEHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]