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The map label for this gene is paaF [H]

Identifier: 186475157

GI number: 186475157

Start: 441843

End: 442607

Strand: Direct

Name: paaF [H]

Synonym: Bphy_0388

Alternate gene names: 186475157

Gene position: 441843-442607 (Clockwise)

Preceding gene: 186475156

Following gene: 186475159

Centisome position: 12.7

GC content: 63.27

Gene sequence:

>765_bases
GTGGAAATCGAACTGACCCGCACGCTCGACGTGCTAACGATCACCTTCAATCGCGCAGAAAAGAAAAACGCGATCACGGC
GGCGATGTATCAAACGATGGCCGACGCGCTGGTCGAAGCGCAAACCGACACCGCCATCCGCGCGGTGCTCCTCCGCGGGC
GCGCGGGCATCTTCAGTGCCGGCAACGATCTCGAAGATTTCATGAAGCAGCCGCCCGTCGGCGAAGACGCGCCCGTGTTC
CAGTTCCTGCGCGCGATCAGTTCGGTGGAGAAACCGCTGGTGGCATCGGTAGCGGGCGCCGCCGTCGGCATCGGCACGAC
GCTGCTCCTGCACTGCGATCTCGTCTACGCCGCCGACTCCGCGACGTTTTCGCTCCCATTCGCACAGCTCGGCCTGTGCC
CCGAAGCTGCGTCGTCGTTATTGTTGCAGCGCGTGGCCGGTTATCAGGTAGCGGCCGAAAAGCTGATGCTCGGCGAACCG
TTCGACGCGAAAGAAGCGTATCGCATGGGCTTCGTGAACCGTCTGCTGCCCGCCGACGAAGTCGATGCGTTCGCGCTCCA
GCAGGCACAGAAGCTGGCGGCGCTGCCCGCGTCGTCGCTGCGCGTCACCAAGCAGATGATGAAGCGCGCCGCGCAGCACG
AAATCCAGACGCAAATGACCGACGAAGCCGTGCACTTCGCGAAGATGCTGCTCGCGCCCGAAGCAAAGGAAGCGTTCAAG
GCGTTCTTCGAGAAGCGCAAGCCCGACTTCCGCCAGTTCAGCTGA

Upstream 100 bases:

>100_bases
GCCAACCGCGTGCGCCAGCGCAGGAAAAGGCAAAACACCCGCTTCGCCGGGGAAAGCATTCCACCCGCTTCGCGGAAAGA
AGCACACCAAGGAGACAAAA

Downstream 100 bases:

>100_bases
GCGCACAAGAAGCAGGTATCAGACGGTGTCGTAATCGACGTAACTCGACTCGCGGCAGAAGCTCACCAGCCGCACGCCCG
CTTCGCGTGCAATCGTGATG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MEIELTRTLDVLTITFNRAEKKNAITAAMYQTMADALVEAQTDTAIRAVLLRGRAGIFSAGNDLEDFMKQPPVGEDAPVF
QFLRAISSVEKPLVASVAGAAVGIGTTLLLHCDLVYAADSATFSLPFAQLGLCPEAASSLLLQRVAGYQVAAEKLMLGEP
FDAKEAYRMGFVNRLLPADEVDAFALQQAQKLAALPASSLRVTKQMMKRAAQHEIQTQMTDEAVHFAKMLLAPEAKEAFK
AFFEKRKPDFRQFS

Sequences:

>Translated_254_residues
MEIELTRTLDVLTITFNRAEKKNAITAAMYQTMADALVEAQTDTAIRAVLLRGRAGIFSAGNDLEDFMKQPPVGEDAPVF
QFLRAISSVEKPLVASVAGAAVGIGTTLLLHCDLVYAADSATFSLPFAQLGLCPEAASSLLLQRVAGYQVAAEKLMLGEP
FDAKEAYRMGFVNRLLPADEVDAFALQQAQKLAALPASSLRVTKQMMKRAAQHEIQTQMTDEAVHFAKMLLAPEAKEAFK
AFFEKRKPDFRQFS
>Mature_254_residues
MEIELTRTLDVLTITFNRAEKKNAITAAMYQTMADALVEAQTDTAIRAVLLRGRAGIFSAGNDLEDFMKQPPVGEDAPVF
QFLRAISSVEKPLVASVAGAAVGIGTTLLLHCDLVYAADSATFSLPFAQLGLCPEAASSLLLQRVAGYQVAAEKLMLGEP
FDAKEAYRMGFVNRLLPADEVDAFALQQAQKLAALPASSLRVTKQMMKRAAQHEIQTQMTDEAVHFAKMLLAPEAKEAFK
AFFEKRKPDFRQFS

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=243, Percent_Identity=32.5102880658436, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI260275230, Length=243, Percent_Identity=32.5102880658436, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI260274832, Length=243, Percent_Identity=32.5102880658436, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=28.9795918367347, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=25.2, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4757968, Length=240, Percent_Identity=25, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI49355787, Length=240, Percent_Identity=25, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI25453481, Length=240, Percent_Identity=24.5833333333333, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI51538573, Length=240, Percent_Identity=24.5833333333333, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI68989263, Length=205, Percent_Identity=30.2439024390244, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4757966, Length=245, Percent_Identity=25.3061224489796, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI51538581, Length=245, Percent_Identity=25.3061224489796, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=30.1075268817204, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4502327, Length=250, Percent_Identity=27.6, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI62530384, Length=231, Percent_Identity=24.6753246753247, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI116235487, Length=194, Percent_Identity=26.8041237113402, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221136756, Length=240, Percent_Identity=21.25, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221136753, Length=240, Percent_Identity=21.25, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221307494, Length=240, Percent_Identity=21.25, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI150378541, Length=248, Percent_Identity=27.4193548387097, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI295842266, Length=229, Percent_Identity=24.0174672489083, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=31.6205533596838, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=29.0836653386454, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787660, Length=247, Percent_Identity=32.3886639676113, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=33.0769230769231, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=29.3893129770992, Blast_Score=101, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=31.8725099601594, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=30.0395256916996, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17549921, Length=228, Percent_Identity=33.7719298245614, Blast_Score=113, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=28.125, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=242, Percent_Identity=29.3388429752066, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=261, Percent_Identity=25.6704980842912, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=224, Percent_Identity=25.4464285714286, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=23.6842105263158, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24654903, Length=207, Percent_Identity=34.2995169082126, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=29.0983606557377, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=29.0983606557377, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=31.0756972111554, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=25.9109311740891, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357171, Length=249, Percent_Identity=25.3012048192771, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=26.0089686098655, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650670, Length=198, Percent_Identity=29.2929292929293, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27748; Mature: 27748

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIELTRTLDVLTITFNRAEKKNAITAAMYQTMADALVEAQTDTAIRAVLLRGRAGIFSA
CCEEHEEHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GNDLEDFMKQPPVGEDAPVFQFLRAISSVEKPLVASVAGAAVGIGTTLLLHCDLVYAADS
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
ATFSLPFAQLGLCPEAASSLLLQRVAGYQVAAEKLMLGEPFDAKEAYRMGFVNRLLPADE
CEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
VDAFALQQAQKLAALPASSLRVTKQMMKRAAQHEIQTQMTDEAVHFAKMLLAPEAKEAFK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AFFEKRKPDFRQFS
HHHHHCCCCCCCCC
>Mature Secondary Structure
MEIELTRTLDVLTITFNRAEKKNAITAAMYQTMADALVEAQTDTAIRAVLLRGRAGIFSA
CCEEHEEHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GNDLEDFMKQPPVGEDAPVFQFLRAISSVEKPLVASVAGAAVGIGTTLLLHCDLVYAADS
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
ATFSLPFAQLGLCPEAASSLLLQRVAGYQVAAEKLMLGEPFDAKEAYRMGFVNRLLPADE
CEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
VDAFALQQAQKLAALPASSLRVTKQMMKRAAQHEIQTQMTDEAVHFAKMLLAPEAKEAFK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AFFEKRKPDFRQFS
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]