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The map label for this gene is citA [H]

Identifier: 186474879

GI number: 186474879

Start: 117293

End: 118615

Strand: Direct

Name: citA [H]

Synonym: Bphy_0109

Alternate gene names: 186474879

Gene position: 117293-118615 (Clockwise)

Preceding gene: 186474878

Following gene: 186474882

Centisome position: 3.37

GC content: 63.79

Gene sequence:

>1323_bases
ATGTCACAAGCATCCAGGAGCCTCAATACGCGGGCGGTCACGGCGGCCGTCATCGGTAATGCGCTCGAGTGGTATGACTT
CACTGTCTTCGGTTTCCTCACCGTCGTCATCGCAGAACTCTTCTTTCCCTCCAGCAGCGATTACTCGGCGATCCTTCTCA
CCACGGCAAGCTTCGGCGTCGCGTTCTTCATGCGGCCCGTCGGCGGCATCGTGCTCGGTCTCTATGCGGATCGCGCCGGA
CGCAAGGCCGCGCTGTCGCTCGTGATCGCGCTGATGTCGCTCGGCATTCTGCTGCTCGCCGTCGCGCCGCCTTATTCGGC
GATCGGCGTGGGCGCGCCGCTGCTGATCGTGGTCGGACGCTTGCTGCAAGGCTTCTCCGCGGGCGGCGAGTTCGGCAGTT
CGACGGCGCTGCTGATCGAAGCCGCGCCTTTCTCGAAGCGCGGCTTCTATGGCAGTTGGCAGATGGCAAGCCAGGCGGCC
GCGCTGCTGCTCGGTGCCGTCGTCGGGGCGCTGATGACGCGTGGCCTGTCCCCCGAAGCGCTCAGGTCGTGGGGCTGGCG
CGTGCCGTTTATCCTCGGTCTCGTGATCGGTCCGATAGGTTTCTACATTCGCCGCAATCTCGCGGATTCCGAAGCATTTC
TGCACGCGAAGCAGACCGCGCGCCGCGCGACGCTCGGCGAGCTGTTTGCGCACCATAGCCGCGACGTGCTCTGCGGTCTC
GGCTCGGTGGTGGCGTTGACAGTCACGATCTATGTGTTGATCAGCTATCTACCCACCTTCGCCGTCAGGCAGCTGAAACT
GCCTTACGCGGATTCGTTCACGGCCGTGATCGTCGGCAATCTGCTGCTCACCGTGCTGTCGCCGCTGACAGGCGCGTGGT
CCGATCGGATCGGGCGCAAAGGGCTGTCGCTGTGGTCGCTGGTCATCACGCTGGTGGCGATCTACCCGCTTTTCGTGTGG
CTGGAAGAAGCCCCGAGCGTATCGAAGCTGATCCTCGTGCAGGCCATCCTTTCTATTGCGCTCTCGGGTTATTACGGACC
ATTCGGCGCGCTGATGGCCGAACTCTTTCCCGCGAACGTGCGCTCGACCGGTCTGTCGCTCGCGTACAACTTCGCGGTGA
TGCTGTTCGGCGGCTTCGGCCAGTTCATCGTCACGTGGCTCATCAAGACGACGGGTTCGCCGCTCGCGCCGACCTACTAC
GTGATGTGCGGGCTCACGATCTCGATCGTCGCCGTCGCATGCATGCCGGCCAAACGTCACGCCGATCTCGACGCGATGCG
CAAGCCGCTCGATGCGCTCGAACAGCCGCTGGAGCGGCGTTAG

Upstream 100 bases:

>100_bases
TTTTACACTCGCATGGCGTGCGCGCGGGGCCTGACGGCGGCCCATTCGAACCAAATTCAAATCAACGCACCCGACGCCTC
GACGCAGGCCGGAGACATCG

Downstream 100 bases:

>100_bases
GCGCTAGCTGCTCGCGGGGATCAGCGGCGGCCGCCGGTCGAACCACGGACGCGCCGTTTCCAGTTGCGCGGCGAGCCGCA
GCAGCAGCGCTTCGCCGCCT

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSQASRSLNTRAVTAAVIGNALEWYDFTVFGFLTVVIAELFFPSSSDYSAILLTTASFGVAFFMRPVGGIVLGLYADRAG
RKAALSLVIALMSLGILLLAVAPPYSAIGVGAPLLIVVGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAA
ALLLGAVVGALMTRGLSPEALRSWGWRVPFILGLVIGPIGFYIRRNLADSEAFLHAKQTARRATLGELFAHHSRDVLCGL
GSVVALTVTIYVLISYLPTFAVRQLKLPYADSFTAVIVGNLLLTVLSPLTGAWSDRIGRKGLSLWSLVITLVAIYPLFVW
LEEAPSVSKLILVQAILSIALSGYYGPFGALMAELFPANVRSTGLSLAYNFAVMLFGGFGQFIVTWLIKTTGSPLAPTYY
VMCGLTISIVAVACMPAKRHADLDAMRKPLDALEQPLERR

Sequences:

>Translated_440_residues
MSQASRSLNTRAVTAAVIGNALEWYDFTVFGFLTVVIAELFFPSSSDYSAILLTTASFGVAFFMRPVGGIVLGLYADRAG
RKAALSLVIALMSLGILLLAVAPPYSAIGVGAPLLIVVGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAA
ALLLGAVVGALMTRGLSPEALRSWGWRVPFILGLVIGPIGFYIRRNLADSEAFLHAKQTARRATLGELFAHHSRDVLCGL
GSVVALTVTIYVLISYLPTFAVRQLKLPYADSFTAVIVGNLLLTVLSPLTGAWSDRIGRKGLSLWSLVITLVAIYPLFVW
LEEAPSVSKLILVQAILSIALSGYYGPFGALMAELFPANVRSTGLSLAYNFAVMLFGGFGQFIVTWLIKTTGSPLAPTYY
VMCGLTISIVAVACMPAKRHADLDAMRKPLDALEQPLERR
>Mature_439_residues
SQASRSLNTRAVTAAVIGNALEWYDFTVFGFLTVVIAELFFPSSSDYSAILLTTASFGVAFFMRPVGGIVLGLYADRAGR
KAALSLVIALMSLGILLLAVAPPYSAIGVGAPLLIVVGRLLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAAA
LLLGAVVGALMTRGLSPEALRSWGWRVPFILGLVIGPIGFYIRRNLADSEAFLHAKQTARRATLGELFAHHSRDVLCGLG
SVVALTVTIYVLISYLPTFAVRQLKLPYADSFTAVIVGNLLLTVLSPLTGAWSDRIGRKGLSLWSLVITLVAIYPLFVWL
EEAPSVSKLILVQAILSIALSGYYGPFGALMAELFPANVRSTGLSLAYNFAVMLFGGFGQFIVTWLIKTTGSPLAPTYYV
MCGLTISIVAVACMPAKRHADLDAMRKPLDALEQPLERR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=419, Percent_Identity=30.7875894988067, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788942, Length=392, Percent_Identity=33.4183673469388, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788292, Length=426, Percent_Identity=29.1079812206573, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789941, Length=447, Percent_Identity=33.5570469798658, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082404, Length=371, Percent_Identity=24.2587601078167, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082231, Length=250, Percent_Identity=28.4, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47043; Mature: 46912

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQASRSLNTRAVTAAVIGNALEWYDFTVFGFLTVVIAELFFPSSSDYSAILLTTASFGV
CCCCHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHH
AFFMRPVGGIVLGLYADRAGRKAALSLVIALMSLGILLLAVAPPYSAIGVGAPLLIVVGR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHH
LLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAAALLLGAVVGALMTRGLSPEA
HHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRSWGWRVPFILGLVIGPIGFYIRRNLADSEAFLHAKQTARRATLGELFAHHSRDVLCGL
HHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GSVVALTVTIYVLISYLPTFAVRQLKLPYADSFTAVIVGNLLLTVLSPLTGAWSDRIGRK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GLSLWSLVITLVAIYPLFVWLEEAPSVSKLILVQAILSIALSGYYGPFGALMAELFPANV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
RSTGLSLAYNFAVMLFGGFGQFIVTWLIKTTGSPLAPTYYVMCGLTISIVAVACMPAKRH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHC
ADLDAMRKPLDALEQPLERR
CCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQASRSLNTRAVTAAVIGNALEWYDFTVFGFLTVVIAELFFPSSSDYSAILLTTASFGV
CCCHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHH
AFFMRPVGGIVLGLYADRAGRKAALSLVIALMSLGILLLAVAPPYSAIGVGAPLLIVVGR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHH
LLQGFSAGGEFGSSTALLIEAAPFSKRGFYGSWQMASQAAALLLGAVVGALMTRGLSPEA
HHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRSWGWRVPFILGLVIGPIGFYIRRNLADSEAFLHAKQTARRATLGELFAHHSRDVLCGL
HHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GSVVALTVTIYVLISYLPTFAVRQLKLPYADSFTAVIVGNLLLTVLSPLTGAWSDRIGRK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
GLSLWSLVITLVAIYPLFVWLEEAPSVSKLILVQAILSIALSGYYGPFGALMAELFPANV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
RSTGLSLAYNFAVMLFGGFGQFIVTWLIKTTGSPLAPTYYVMCGLTISIVAVACMPAKRH
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHC
ADLDAMRKPLDALEQPLERR
CCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]