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The map label for this gene is argT [H]

Identifier: 186474788

GI number: 186474788

Start: 18797

End: 19588

Strand: Direct

Name: argT [H]

Synonym: Bphy_0016

Alternate gene names: 186474788

Gene position: 18797-19588 (Clockwise)

Preceding gene: 186474787

Following gene: 186474789

Centisome position: 0.54

GC content: 62.25

Gene sequence:

>792_bases
ATGAAGCTGGTTAAGAGTCTGCTGATGCTGTCGTGCGGGTTCGCATCGGTGGCGGCCGTGTCGGCGATAGCCGCCGACGC
GACGTCGCTGCGCTATGGCCTCGAGGCGCAATATCCGCCGTTTGAATCGAAGGGTGCGAATGGCGAGTTGCAGGGCTTCG
ATATCGATATCGGCAACGCCGTCTGCAAGACCGCAAATCTGAAATGCAGCTGGGTTGAAACGTCGTTCGACGGCCTGATT
CCCGCTTTGCAGGGCCGCAAGTTCGACGCGATCAACTCGGCGATGAACGCTACGGAAAAGCGCCGCCAGGCGATTGATTT
CACCAACGTGATCTACCGCGTGCCGACGCAACTGATCGCGCGCAAGGACAGCGGACTGCAGCCGACGCCCGAATCTCTGA
AGGGCAAGCGCGTCGGCGTGTTGCAGGCGTCGATTCAGGAGACGTATGCGAAGGCCCACTGGGAAAGCGCGGGCGTCGCT
GTGGTGCCGTATCAGGACCAGAACCAGGTGTACGCGGATCTCGTCGCCGGCCGCCTCGATGCGACGCTCGTGCTCGCGCC
GGCTGGGCAGACGGGTTTCCTGTCACGCCCTGACGGCAAGGACTTCGCGTTCGTCGGCGAGCCGGTGCGTGACGACAAGA
TTCTCGGCAGCGGCATCGCATTCGGGATTCGCAAGGGCGACACGGCGCTGCGCAACCAGCTGAACGCGGCGATCGCCAAG
GTTCAGTCGGAGGGCACGGTCAAGACGCTCGCGCAAAAGTACTTCGGCAATATCGACGTATCGGCGAAGTAA

Upstream 100 bases:

>100_bases
CGAAGCGCGCGACCGTCTGCCGGTGCTCGCGCATCGGCGCATCGCAACGCCGTCTGACGTGCGTTGAACCGCATTGAACA
CAAGGGGAAGGAGATCGATG

Downstream 100 bases:

>100_bases
GTCTGCGCTCTTCACACAGATACGCGCCGCGTGGTTTTCGCCACGCGGCGCGTTTCTTTTGCGTAACGTCTGACGAATGA
ATCACTGAAAGCCGATGGAA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKLVKSLLMLSCGFASVAAVSAIAADATSLRYGLEAQYPPFESKGANGELQGFDIDIGNAVCKTANLKCSWVETSFDGLI
PALQGRKFDAINSAMNATEKRRQAIDFTNVIYRVPTQLIARKDSGLQPTPESLKGKRVGVLQASIQETYAKAHWESAGVA
VVPYQDQNQVYADLVAGRLDATLVLAPAGQTGFLSRPDGKDFAFVGEPVRDDKILGSGIAFGIRKGDTALRNQLNAAIAK
VQSEGTVKTLAQKYFGNIDVSAK

Sequences:

>Translated_263_residues
MKLVKSLLMLSCGFASVAAVSAIAADATSLRYGLEAQYPPFESKGANGELQGFDIDIGNAVCKTANLKCSWVETSFDGLI
PALQGRKFDAINSAMNATEKRRQAIDFTNVIYRVPTQLIARKDSGLQPTPESLKGKRVGVLQASIQETYAKAHWESAGVA
VVPYQDQNQVYADLVAGRLDATLVLAPAGQTGFLSRPDGKDFAFVGEPVRDDKILGSGIAFGIRKGDTALRNQLNAAIAK
VQSEGTVKTLAQKYFGNIDVSAK
>Mature_263_residues
MKLVKSLLMLSCGFASVAAVSAIAADATSLRYGLEAQYPPFESKGANGELQGFDIDIGNAVCKTANLKCSWVETSFDGLI
PALQGRKFDAINSAMNATEKRRQAIDFTNVIYRVPTQLIARKDSGLQPTPESLKGKRVGVLQASIQETYAKAHWESAGVA
VVPYQDQNQVYADLVAGRLDATLVLAPAGQTGFLSRPDGKDFAFVGEPVRDDKILGSGIAFGIRKGDTALRNQLNAAIAK
VQSEGTVKTLAQKYFGNIDVSAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=47.8260869565217, Blast_Score=240, Evalue=6e-65,
Organism=Escherichia coli, GI1788648, Length=227, Percent_Identity=48.0176211453744, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI1787088, Length=244, Percent_Identity=32.7868852459016, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787085, Length=256, Percent_Identity=32.421875, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1788228, Length=229, Percent_Identity=31.4410480349345, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787031, Length=254, Percent_Identity=29.5275590551181, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28104; Mature: 28104

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVKSLLMLSCGFASVAAVSAIAADATSLRYGLEAQYPPFESKGANGELQGFDIDIGNA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCH
VCKTANLKCSWVETSFDGLIPALQGRKFDAINSAMNATEKRRQAIDFTNVIYRVPTQLIA
HHHCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKDSGLQPTPESLKGKRVGVLQASIQETYAKAHWESAGVAVVPYQDQNQVYADLVAGRLD
HCCCCCCCCCHHHCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCC
ATLVLAPAGQTGFLSRPDGKDFAFVGEPVRDDKILGSGIAFGIRKGDTALRNQLNAAIAK
EEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHH
VQSEGTVKTLAQKYFGNIDVSAK
HCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLVKSLLMLSCGFASVAAVSAIAADATSLRYGLEAQYPPFESKGANGELQGFDIDIGNA
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCH
VCKTANLKCSWVETSFDGLIPALQGRKFDAINSAMNATEKRRQAIDFTNVIYRVPTQLIA
HHHCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKDSGLQPTPESLKGKRVGVLQASIQETYAKAHWESAGVAVVPYQDQNQVYADLVAGRLD
HCCCCCCCCCHHHCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCC
ATLVLAPAGQTGFLSRPDGKDFAFVGEPVRDDKILGSGIAFGIRKGDTALRNQLNAAIAK
EEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHH
VQSEGTVKTLAQKYFGNIDVSAK
HCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]