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The map label for this gene is gyrB [H]

Identifier: 186474776

GI number: 186474776

Start: 3392

End: 5863

Strand: Direct

Name: gyrB [H]

Synonym: Bphy_0003

Alternate gene names: 186474776

Gene position: 3392-5863 (Clockwise)

Preceding gene: 186474775

Following gene: 186474778

Centisome position: 0.1

GC content: 60.15

Gene sequence:

>2472_bases
ATGACTGATACGAACAACTCGCAACCCGATAACAGCTACGGCGCCTCATCCATTCAGATCCTCGAAGGTCTGGAGGCCGT
GCGCAAGCGGCCGGGTATGTACATCGGCGATACGTCGGACGGCACCGGTCTGCATCACCTCGTGTTCGAGGTGCTCGACA
ATTCGATCGACGAGGCGCTCGCCGGTCATTGCGACGACATTCAGGTCATTATCCACGCCGACAACTCGATCTCCGTGACC
GACAATGGCCGCGGCATTCCGACGGGCATCAAGCGCGACGACAAGCACGATCCAAAGCGCAGCGCCGCTGAGATTGTGAT
GACGGAACTGCATGCAGGCGGAAAGTTCGACCAGAACAGTTACAAGGTGTCGGGCGGCCTGCACGGTGTGGGTGTGTCGT
GTGTGAACGCGCTGTCGTCCTGGCTGCGCCTTACCGTGCGCCGCGACGGCAAGAAGCACTTCATGGAGTTTCACCGTGGC
GTGGTGCAGAACCGCGTGATCGAGCAAGTGGACGGCGAAAGTCTGTCGCCCATTCCCGTTGTGGGCGAGACGGAAAATCG
CGGCACTGAAGTGCACTTCATGGCCGACGAGACGATCTTCGGCAACGTCGAATACCACTACGACATTCTGGCGAAGCGTA
TCCGCGAACTCTCATTCCTGAACAACGGCGTACGGATTCGTCTCACCGATCAGCGTTCGGGCAAGGAAGATGATTTCGCC
TTTGTCGGCGGCGTGAAGGGCTTTGTCGAGTACATCAACAAGACGAAGAGCGTACTGCATCCGACCATCTTCCATATTGT
CGGCGAGAAGGAGGGCGTGGGCGTCGAAGTCGCGATGCAGTGGAACGACAGCTACAACGAAAACGTGCTGTGTTTCACGA
ACAACATTCCGCAGCGCGATGGCGGCACGCACCTTACCGGTCTGCGCGCGGCGATGACGCGCGTGATCAACAAGTACATT
GCCGATCACGAAATCGCGAAAAAGGCGAAGGTCGAAACGTCCGGCGACGACATGCGCGAAGGGTTGTCGTGCGTGCTGTC
GGTGAAGGTGCCGGAGCCGAAGTTCAGCTCGCAGACGAAGGACAAGCTGGTTTCGTCGGAAGTGCGCGCACCCGTGGAAG
AGGTGGTCGCGAAGGCGCTAGAAGAATTCCTGCTCGAAACGCCGAACGACGCAAAGATCATTTGCGGCAAGATCGTCGAT
GCCGCGCGCGCGCGCGATGCAGCGCGCAAGGCGCGAGAAATGACGCGACGCAAGGGCGTGCTGGATGGCGTCGGTCTACC
GGGCAAGCTTGCGGACTGCCAGGAGAAAGATCCGGCGAAGTCCGAGATATATATCGTCGAGGGTGACTCGGCAGGCGGTT
CGGCGAAGCAGGGCCGTGACAGAAAATTCCAAGCCATTCTGCCGCTGCGCGGCAAGGTGTTGAACGTCGAGAAGGCGCGC
TACGACAAGCTGCTGTCGTCGGAGCAGATCGTCACGCTGATCACCGCATTGGGTTGCGGCATCGGCAAGGAAGACTACAA
CCTCGACAAGCTGCGTTATCACCGCATCATCATCATGACCGACGCTGACGTCGACGGCGCGCACATCCGTACGCTGCTGC
TGACGTTCTTCTATCGTCAGATGCCGGAGATGATCGAGCGCGGCTACATCTACATCGCGCAGCCGCCGCTGTACAAGGTG
AAGGCGGGCAAGGATGAGCGCTATCTTAAGGACACCGCCGAGTTGAACGCGCATATGCTGCGTCTGGCTCTGAACGGTTC
TGAACTGGTCGCCGCCGAAGGTGCGACGCCGATCGCCGGCGACGCGCTCGGCGAGCTGGCGCGTTCTTATCTGCTGGCGC
AGGCTGTCGTCGATCGTCTGAGCCGGCTGTACGACGAGGCGGCGCTCGGGGCGGTGATGGATGGCGTGTCGATCGATCTG
TCGAGCGAGCAGTCAACGGAAGCATCGGCGCGCGCGCTCGAAGCCAAGCTGCGCGACGATCCGCTCAAGCCCGAGATCAA
CGTGTTCGCGATGTACGACCCGGTGCGCGAACTGCGTTCGCTGCGGGTGGAGCGTCGCCATCACGGCAACGTGAAGGTCT
CGGTCATCGATGAGGAGTTCCAGCTGACGGCCGACTATCAGCAACTCGTGAACACGGCCAATACGTTCAAGGGCTTGATC
GGCGAGGGCGCTGTCATCAAGCGCGGCGAGCGCAGCATGGCCGTGGGCGACTTCAAGAGCGCGATGAAGTGGCTTATCGC
CGATGCGGAACGCAACGTGTCGAAGCAGCGGTATAAGGGACTCGGCGAGATGAATCCCGAACAGCTGTGGGAAACGACGA
TGGATCCGAACGTGCGTCGTTTGCTGCGCGTGCAGATCGAAGACGCGATTGCGGCCGATGGGATCTTTACGACGCTGATG
GGCGATGAGGTCGAGCCGCGCCGCGCGTTTATCGAGAGCAATGCATTGCGGGCAGGGAATATTGACGTTTAA

Upstream 100 bases:

>100_bases
AAGAACACACCAAGGGGCGCAGCGCCCCTTTGGCGTTTTTATGGTGTTTTGAAATTCCTCGAGCAGTAACGAAGCAGCAA
CGCAGATCCGGAAGAAATCC

Downstream 100 bases:

>100_bases
GCTGGCTGGCTGGCACACAGCGCTCCGTCCTGGGCGCGTCAGTAGTCAAATGAAAAACGCCAACCTATTCCGGTTGGCGT
TTTTTTTGCACATCAATCAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 823; Mature: 822

Protein sequence:

>823_residues
MTDTNNSQPDNSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCDDIQVIIHADNSISVT
DNGRGIPTGIKRDDKHDPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKHFMEFHRG
VVQNRVIEQVDGESLSPIPVVGETENRGTEVHFMADETIFGNVEYHYDILAKRIRELSFLNNGVRIRLTDQRSGKEDDFA
FVGGVKGFVEYINKTKSVLHPTIFHIVGEKEGVGVEVAMQWNDSYNENVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYI
ADHEIAKKAKVETSGDDMREGLSCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEEVVAKALEEFLLETPNDAKIICGKIVD
AARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPAKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKAR
YDKLLSSEQIVTLITALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPEMIERGYIYIAQPPLYKV
KAGKDERYLKDTAELNAHMLRLALNGSELVAAEGATPIAGDALGELARSYLLAQAVVDRLSRLYDEAALGAVMDGVSIDL
SSEQSTEASARALEAKLRDDPLKPEINVFAMYDPVRELRSLRVERRHHGNVKVSVIDEEFQLTADYQQLVNTANTFKGLI
GEGAVIKRGERSMAVGDFKSAMKWLIADAERNVSKQRYKGLGEMNPEQLWETTMDPNVRRLLRVQIEDAIAADGIFTTLM
GDEVEPRRAFIESNALRAGNIDV

Sequences:

>Translated_823_residues
MTDTNNSQPDNSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCDDIQVIIHADNSISVT
DNGRGIPTGIKRDDKHDPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKHFMEFHRG
VVQNRVIEQVDGESLSPIPVVGETENRGTEVHFMADETIFGNVEYHYDILAKRIRELSFLNNGVRIRLTDQRSGKEDDFA
FVGGVKGFVEYINKTKSVLHPTIFHIVGEKEGVGVEVAMQWNDSYNENVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYI
ADHEIAKKAKVETSGDDMREGLSCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEEVVAKALEEFLLETPNDAKIICGKIVD
AARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPAKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKAR
YDKLLSSEQIVTLITALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPEMIERGYIYIAQPPLYKV
KAGKDERYLKDTAELNAHMLRLALNGSELVAAEGATPIAGDALGELARSYLLAQAVVDRLSRLYDEAALGAVMDGVSIDL
SSEQSTEASARALEAKLRDDPLKPEINVFAMYDPVRELRSLRVERRHHGNVKVSVIDEEFQLTADYQQLVNTANTFKGLI
GEGAVIKRGERSMAVGDFKSAMKWLIADAERNVSKQRYKGLGEMNPEQLWETTMDPNVRRLLRVQIEDAIAADGIFTTLM
GDEVEPRRAFIESNALRAGNIDV
>Mature_822_residues
TDTNNSQPDNSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEALAGHCDDIQVIIHADNSISVTD
NGRGIPTGIKRDDKHDPKRSAAEIVMTELHAGGKFDQNSYKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKHFMEFHRGV
VQNRVIEQVDGESLSPIPVVGETENRGTEVHFMADETIFGNVEYHYDILAKRIRELSFLNNGVRIRLTDQRSGKEDDFAF
VGGVKGFVEYINKTKSVLHPTIFHIVGEKEGVGVEVAMQWNDSYNENVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIA
DHEIAKKAKVETSGDDMREGLSCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEEVVAKALEEFLLETPNDAKIICGKIVDA
ARARDAARKAREMTRRKGVLDGVGLPGKLADCQEKDPAKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKARY
DKLLSSEQIVTLITALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPEMIERGYIYIAQPPLYKVK
AGKDERYLKDTAELNAHMLRLALNGSELVAAEGATPIAGDALGELARSYLLAQAVVDRLSRLYDEAALGAVMDGVSIDLS
SEQSTEASARALEAKLRDDPLKPEINVFAMYDPVRELRSLRVERRHHGNVKVSVIDEEFQLTADYQQLVNTANTFKGLIG
EGAVIKRGERSMAVGDFKSAMKWLIADAERNVSKQRYKGLGEMNPEQLWETTMDPNVRRLLRVQIEDAIAADGIFTTLMG
DEVEPRRAFIESNALRAGNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=570, Percent_Identity=23.6842105263158, Blast_Score=117, Evalue=6e-26,
Organism=Homo sapiens, GI19913406, Length=559, Percent_Identity=24.1502683363148, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI48994957, Length=825, Percent_Identity=59.6363636363636, Blast_Score=967, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=563, Percent_Identity=37.8330373001776, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17535065, Length=596, Percent_Identity=23.1543624161074, Blast_Score=119, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=525, Percent_Identity=25.1428571428571, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI212645657, Length=159, Percent_Identity=28.9308176100629, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=612, Percent_Identity=25.4901960784314, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17136538, Length=546, Percent_Identity=24.5421245421245, Blast_Score=112, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91080; Mature: 90948

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTNNSQPDNSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEAL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AGHCDDIQVIIHADNSISVTDNGRGIPTGIKRDDKHDPKRSAAEIVMTELHAGGKFDQNS
CCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKHFMEFHRGVVQNRVIEQVDGESLSPIPV
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VGETENRGTEVHFMADETIFGNVEYHYDILAKRIRELSFLNNGVRIRLTDQRSGKEDDFA
ECCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEE
FVGGVKGFVEYINKTKSVLHPTIFHIVGEKEGVGVEVAMQWNDSYNENVLCFTNNIPQRD
EECHHHHHHHHHHHHHHHHCHHHHHEECCCCCCCEEEEEEECCCCCCCEEEEECCCCCCC
GGTHLTGLRAAMTRVINKYIADHEIAKKAKVETSGDDMREGLSCVLSVKVPEPKFSSQTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCEEEEEEECCCCCCCCHHH
DKLVSSEVRAPVEEVVAKALEEFLLETPNDAKIICGKIVDAARARDAARKAREMTRRKGV
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
LDGVGLPGKLADCQEKDPAKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKAR
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEECHHHH
YDKLLSSEQIVTLITALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQ
HHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHH
MPEMIERGYIYIAQPPLYKVKAGKDERYLKDTAELNAHMLRLALNGSELVAAEGATPIAG
HHHHHHCCEEEEECCCCEEEECCCCHHHHHHHHHHHHEEEEEEECCCEEEEECCCCCCCH
DALGELARSYLLAQAVVDRLSRLYDEAALGAVMDGVSIDLSSEQSTEASARALEAKLRDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCC
PLKPEINVFAMYDPVRELRSLRVERRHHGNVKVSVIDEEFQLTADYQQLVNTANTFKGLI
CCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
GEGAVIKRGERSMAVGDFKSAMKWLIADAERNVSKQRYKGLGEMNPEQLWETTMDPNVRR
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHH
LLRVQIEDAIAADGIFTTLMGDEVEPRRAFIESNALRAGNIDV
HHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TDTNNSQPDNSYGASSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVLDNSIDEAL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AGHCDDIQVIIHADNSISVTDNGRGIPTGIKRDDKHDPKRSAAEIVMTELHAGGKFDQNS
CCCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YKVSGGLHGVGVSCVNALSSWLRLTVRRDGKKHFMEFHRGVVQNRVIEQVDGESLSPIPV
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VGETENRGTEVHFMADETIFGNVEYHYDILAKRIRELSFLNNGVRIRLTDQRSGKEDDFA
ECCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEE
FVGGVKGFVEYINKTKSVLHPTIFHIVGEKEGVGVEVAMQWNDSYNENVLCFTNNIPQRD
EECHHHHHHHHHHHHHHHHCHHHHHEECCCCCCCEEEEEEECCCCCCCEEEEECCCCCCC
GGTHLTGLRAAMTRVINKYIADHEIAKKAKVETSGDDMREGLSCVLSVKVPEPKFSSQTK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCEEEEEEECCCCCCCCHHH
DKLVSSEVRAPVEEVVAKALEEFLLETPNDAKIICGKIVDAARARDAARKAREMTRRKGV
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
LDGVGLPGKLADCQEKDPAKSEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKVLNVEKAR
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEECHHHH
YDKLLSSEQIVTLITALGCGIGKEDYNLDKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQ
HHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHH
MPEMIERGYIYIAQPPLYKVKAGKDERYLKDTAELNAHMLRLALNGSELVAAEGATPIAG
HHHHHHCCEEEEECCCCEEEECCCCHHHHHHHHHHHHEEEEEEECCCEEEEECCCCCCCH
DALGELARSYLLAQAVVDRLSRLYDEAALGAVMDGVSIDLSSEQSTEASARALEAKLRDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCC
PLKPEINVFAMYDPVRELRSLRVERRHHGNVKVSVIDEEFQLTADYQQLVNTANTFKGLI
CCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
GEGAVIKRGERSMAVGDFKSAMKWLIADAERNVSKQRYKGLGEMNPEQLWETTMDPNVRR
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHH
LLRVQIEDAIAADGIFTTLMGDEVEPRRAFIESNALRAGNIDV
HHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1906260 [H]