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The map label for this gene is dnaN [H]

Identifier: 186474775

GI number: 186474775

Start: 2175

End: 3278

Strand: Direct

Name: dnaN [H]

Synonym: Bphy_0002

Alternate gene names: 186474775

Gene position: 2175-3278 (Clockwise)

Preceding gene: 186474774

Following gene: 186474776

Centisome position: 0.06

GC content: 59.51

Gene sequence:

>1104_bases
ATGCAACTGGTCAAGACCGAACGCGATAACCTCCTCAGGCCGCTGCAAACCGTGAGCGGCATTGTCGAACGCCGTCATAC
GTTGCCGATCCTCGCCAATTTGCTCATCACCAAGAACGGCCCGGACGTTTCGTTCCTGTCCACCGACCTCGAACTGCAGA
TCACCACGCGTGCCGACTTCGGTGTCGGCGGTGAATCGGTGGCCACCACGGTGGCGGCGCGCAAGCTGCTCGACATTCTG
CGCGCGATGCCCGACGGGCAGGTCACACTCACCCTGAACGACAAGCGTCTGACGGTTCAGTCCGGCAAGAGCCGTTTCGC
GCTGCAGACGCTGGCGGCCGACGAGTTCCCGACCGTCGCTCAAGCTAAAGACTTCGGCGCGAACCTCGCGGTTCCCCAAA
AAACGTTCCGCCAGTTGCTCGGCATGGTTCATTTCGCGATGGCGCAGCAGGACATCCGTTACTACCTGAACGGCATGTTG
CTGGTGGTCGATGGCGACCAGCTGATGGCCGTGGCAACGGACGGTCACCGTCTCGCGTATTCGTCGATGAAGATCGAAGG
CTCGTTTGCGCGCCAGGAAGTCATCATCCCGCGTAAGACGATTCTCGAACTTCAACGTCTGCTCGAAGATATCGACGACG
TGCTCAAGATCGACATCGCGCCGACGCAGGTGAAGTTCACCTTCGGCGGCGTCGAACTGGTGTCGAAGCTGGTCGAAGGC
AAGTTCCCCGACTTTCAGCGCGTGATCCCGAAGTCGCACAAAAACACGTTCGTGATCGGTCGGGAAGAACTGCAGCGCTC
GCTGCAACGCGCGGCCATTCTGACGTCGGACAAGTTCAAGGGTGTGCGCTGCATCGTCGAGCCGGGCCAGCTGAAGATCA
TGTCCACCAACGCGGACCAGGAAGAAGCGCAGGAAGAACTCGAAATCCCGTATCAGGGCGACAGCATCGACATCGGTTTC
AACGTCACGTATCTGCTCGACGTGCTCGCGAACCTGAAGGTCGACATGCTGGAAATCAGCCTCGGCGACGCCAGTTCGAG
CGCGCTGATCACGATTCCCGAGAACGACGAGTTCAAATACGTGGTGATGCCGATGCGCATCTGA

Upstream 100 bases:

>100_bases
GCCGTGACTGGCGCAGTACGACGCCGGCGGGGGGCCGGGGCCGCGCGCTTCAATCTAGCCGGACAAGGCGCGCAGGCCGT
CACATCAACGAAGGAACTCT

Downstream 100 bases:

>100_bases
CGCGTCCAACACGAAGAACACACCAAGGGGCGCAGCGCCCCTTTGGCGTTTTTATGGTGTTTTGAAATTCCTCGAGCAGT
AACGAAGCAGCAACGCAGAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGESVATTVAARKLLDIL
RAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGANLAVPQKTFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAYSSMKIEGSFARQEVIIPRKTILELQRLLEDIDDVLKIDIAPTQVKFTFGGVELVSKLVEG
KFPDFQRVIPKSHKNTFVIGREELQRSLQRAAILTSDKFKGVRCIVEPGQLKIMSTNADQEEAQEELEIPYQGDSIDIGF
NVTYLLDVLANLKVDMLEISLGDASSSALITIPENDEFKYVVMPMRI

Sequences:

>Translated_367_residues
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGESVATTVAARKLLDIL
RAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGANLAVPQKTFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAYSSMKIEGSFARQEVIIPRKTILELQRLLEDIDDVLKIDIAPTQVKFTFGGVELVSKLVEG
KFPDFQRVIPKSHKNTFVIGREELQRSLQRAAILTSDKFKGVRCIVEPGQLKIMSTNADQEEAQEELEIPYQGDSIDIGF
NVTYLLDVLANLKVDMLEISLGDASSSALITIPENDEFKYVVMPMRI
>Mature_367_residues
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGESVATTVAARKLLDIL
RAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGANLAVPQKTFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAYSSMKIEGSFARQEVIIPRKTILELQRLLEDIDDVLKIDIAPTQVKFTFGGVELVSKLVEG
KFPDFQRVIPKSHKNTFVIGREELQRSLQRAAILTSDKFKGVRCIVEPGQLKIMSTNADQEEAQEELEIPYQGDSIDIGF
NVTYLLDVLANLKVDMLEISLGDASSSALITIPENDEFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=362, Percent_Identity=43.9226519337017, Blast_Score=304, Evalue=7e-84,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40821; Mature: 40821

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGESVATTVAARKLLDILRAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVA
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEHHHHCCCCCCCHH
QAKDFGANLAVPQKTFRQLLGMVHFAMAQQDIRYYLNGMLLVVDGDQLMAVATDGHRLAY
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
SSMKIEGSFARQEVIIPRKTILELQRLLEDIDDVLKIDIAPTQVKFTFGGVELVSKLVEG
EEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECHHHHHHHHHCC
KFPDFQRVIPKSHKNTFVIGREELQRSLQRAAILTSDKFKGVRCIVEPGQLKIMSTNADQ
CCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCH
EEAQEELEIPYQGDSIDIGFNVTYLLDVLANLKVDMLEISLGDASSSALITIPENDEFKY
HHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCCCEEE
VVMPMRI
EEEEECC
>Mature Secondary Structure
MQLVKTERDNLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGESVATTVAARKLLDILRAMPDGQVTLTLNDKRLTVQSGKSRFALQTLAADEFPTVA
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCEEHHHHCCCCCCCHH
QAKDFGANLAVPQKTFRQLLGMVHFAMAQQDIRYYLNGMLLVVDGDQLMAVATDGHRLAY
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEEECCCEEEE
SSMKIEGSFARQEVIIPRKTILELQRLLEDIDDVLKIDIAPTQVKFTFGGVELVSKLVEG
EEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECHHHHHHHHHCC
KFPDFQRVIPKSHKNTFVIGREELQRSLQRAAILTSDKFKGVRCIVEPGQLKIMSTNADQ
CCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCH
EEAQEELEIPYQGDSIDIGFNVTYLLDVLANLKVDMLEISLGDASSSALITIPENDEFKY
HHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCCCCCEEE
VVMPMRI
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]