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The map label for this gene is grpE

Identifier: 183222724

GI number: 183222724

Start: 3500043

End: 3500615

Strand: Reverse

Name: grpE

Synonym: LEPBI_I3380

Alternate gene names: 183222724

Gene position: 3500615-3500043 (Counterclockwise)

Preceding gene: 183222725

Following gene: 183222723

Centisome position: 97.25

GC content: 41.54

Gene sequence:

>573_bases
ATGGCAGAAGAAACGAACCAATCCCTAGAAGATCAAAATGTGCAAGTCGAAGAAGGGCAGACCATTTCAGATGAGGCCAT
TGAACAAGCGGTAGAAGGTGCTGAAAAAGAACTCGATAACGCTAAAAAAGAAATCGAGTCCTTAAAAGATTCCTGGCTAA
GAGAACGTGCGGAGTTTCAAAACTACAAACGTAGAACAGCAAACGACTTGTTGAATGCTAGGAAAGAATCCATCAAAAAG
TTTGCGGAAGGACTCACAGGTGCTTTGGACAATTTGGAACGTGTATCAAATGTTCCAAACCAAACACCAGAAGTAGTCGC
CTTTGTTGAAGGGATCAAGATGGTACAAAAGGAATTTTATTCCGTTTTGGAAAAAGAAGGAATCAAACGTTTGGATCCAA
AAGGACAACCGTTTGACCCTATGCTCATGGAAGCAATTGCTTCCGAGGAAAGTGCAGAATTTACGGAAGAGACAGTTGTC
GAAACCTACCAAGCTGGTTATTACCATGAAGAAGGTGAAAGCAAACAATCCATTCGTCCTGCACGTGTAAAAGTGGGAAA
ACCACAAAGTTAA

Upstream 100 bases:

>100_bases
CTCAGAGGATGAATTACAACAAAGCATTACCTTTGATTGAGTTCACTTCAAAGTTAGTTTCAGAAATGATAACGAAATTG
AGCAGGTAGTAGGAGGCGAA

Downstream 100 bases:

>100_bases
TATAAGTAAGAAGGAGAAAGACTATGTCTAAGGAAAAAATCATAGGTATCGATTTAGGAACCACTAACTCATGTGTGGCG
GTAATGGAAGGTGGTGATCC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MAEETNQSLEDQNVQVEEGQTISDEAIEQAVEGAEKELDNAKKEIESLKDSWLRERAEFQNYKRRTANDLLNARKESIKK
FAEGLTGALDNLERVSNVPNQTPEVVAFVEGIKMVQKEFYSVLEKEGIKRLDPKGQPFDPMLMEAIASEESAEFTEETVV
ETYQAGYYHEEGESKQSIRPARVKVGKPQS

Sequences:

>Translated_190_residues
MAEETNQSLEDQNVQVEEGQTISDEAIEQAVEGAEKELDNAKKEIESLKDSWLRERAEFQNYKRRTANDLLNARKESIKK
FAEGLTGALDNLERVSNVPNQTPEVVAFVEGIKMVQKEFYSVLEKEGIKRLDPKGQPFDPMLMEAIASEESAEFTEETVV
ETYQAGYYHEEGESKQSIRPARVKVGKPQS
>Mature_189_residues
AEETNQSLEDQNVQVEEGQTISDEAIEQAVEGAEKELDNAKKEIESLKDSWLRERAEFQNYKRRTANDLLNARKESIKKF
AEGLTGALDNLERVSNVPNQTPEVVAFVEGIKMVQKEFYSVLEKEGIKRLDPKGQPFDPMLMEAIASEESAEFTEETVVE
TYQAGYYHEEGESKQSIRPARVKVGKPQS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=143, Percent_Identity=32.8671328671329, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=162, Percent_Identity=30.8641975308642, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653432, Length=163, Percent_Identity=31.2883435582822, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_LEPBA (B0SHT2)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964311.1
- ProteinModelPortal:   B0SHT2
- SMR:   B0SHT2
- GeneID:   6388094
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3266
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- ProtClustDB:   PRK14146
- BioCyc:   LBIF355278:LBF_3266-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21531; Mature: 21400

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEETNQSLEDQNVQVEEGQTISDEAIEQAVEGAEKELDNAKKEIESLKDSWLRERAEFQ
CCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYKRRTANDLLNARKESIKKFAEGLTGALDNLERVSNVPNQTPEVVAFVEGIKMVQKEFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SVLEKEGIKRLDPKGQPFDPMLMEAIASEESAEFTEETVVETYQAGYYHEEGESKQSIRP
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCH
ARVKVGKPQS
HHHCCCCCCC
>Mature Secondary Structure 
AEETNQSLEDQNVQVEEGQTISDEAIEQAVEGAEKELDNAKKEIESLKDSWLRERAEFQ
CCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYKRRTANDLLNARKESIKKFAEGLTGALDNLERVSNVPNQTPEVVAFVEGIKMVQKEFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
SVLEKEGIKRLDPKGQPFDPMLMEAIASEESAEFTEETVVETYQAGYYHEEGESKQSIRP
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCH
ARVKVGKPQS
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA