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The map label for this gene is pspE [H]

Identifier: 183222649

GI number: 183222649

Start: 3417178

End: 3417534

Strand: Reverse

Name: pspE [H]

Synonym: LEPBI_I3304

Alternate gene names: 183222649

Gene position: 3417534-3417178 (Counterclockwise)

Preceding gene: 183222650

Following gene: 183222647

Centisome position: 94.94

GC content: 40.06

Gene sequence:

>357_bases
TTGAATCGAATGATCCTAATCATCATTGCCATTGTTGCTGTTTTATTCATTGTTTACCAATTGAGAAATGTTTCGGGTAT
GGACCAGTCACAACTCAAAGAAAAAATTGATGCTGGTGCTCTTGTGGTGGATGTTCGCACAGTAACAGAATTCAATTCTG
GTCATTTTCCCAATGCACGTAATATTCCAATTGATGAAGTTTCCAAACGAGTGGATGAGTTTGGTGACAAAAACCAAACC
ATCATCGTCTATTGTGCGTCAGGTGGACGGAGTGGTAGTGCGAAATCCTTTTTAGAATCCATTGGATACAAACAAGTGAT
CAACGCCGGTGGACTTTCGAATATGCCAAATCCGTAA

Upstream 100 bases:

>100_bases
GTGCCGCCTGCCACACAGGTTATTATTCCTGTTTTTTTAGAGAACTCGGTCAGAATGAATCTGTTTCATTCGTCTATTCT
AATAAAGTCGGAGAGTCAGT

Downstream 100 bases:

>100_bases
TTGGATTTGGCAAGTTTTCTATTATCTAGATTTTTTTCCCTTTCAGATCTCGATTTCCAATTTCCATTTCATCGAAATTC
ATCTCCAACCAAAACTACAT

Product: putative phage shock protein E

Products: NA

Alternate protein names: TST; Phage shock protein E [H]

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MNRMILIIIAIVAVLFIVYQLRNVSGMDQSQLKEKIDAGALVVDVRTVTEFNSGHFPNARNIPIDEVSKRVDEFGDKNQT
IIVYCASGGRSGSAKSFLESIGYKQVINAGGLSNMPNP

Sequences:

>Translated_118_residues
MNRMILIIIAIVAVLFIVYQLRNVSGMDQSQLKEKIDAGALVVDVRTVTEFNSGHFPNARNIPIDEVSKRVDEFGDKNQT
IIVYCASGGRSGSAKSFLESIGYKQVINAGGLSNMPNP
>Mature_118_residues
MNRMILIIIAIVAVLFIVYQLRNVSGMDQSQLKEKIDAGALVVDVRTVTEFNSGHFPNARNIPIDEVSKRVDEFGDKNQT
IIVYCASGGRSGSAKSFLESIGYKQVINAGGLSNMPNP

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyan

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787567, Length=77, Percent_Identity=42.8571428571429, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014323
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 12873; Mature: 12873

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRMILIIIAIVAVLFIVYQLRNVSGMDQSQLKEKIDAGALVVDVRTVTEFNSGHFPNAR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCC
NIPIDEVSKRVDEFGDKNQTIIVYCASGGRSGSAKSFLESIGYKQVINAGGLSNMPNP
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNRMILIIIAIVAVLFIVYQLRNVSGMDQSQLKEKIDAGALVVDVRTVTEFNSGHFPNAR
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCC
NIPIDEVSKRVDEFGDKNQTIIVYCASGGRSGSAKSFLESIGYKQVINAGGLSNMPNP
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1712397; 9097039; 9278503; 9868784 [H]