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The map label for this gene is birA [H]

Identifier: 183222618

GI number: 183222618

Start: 3386330

End: 3387085

Strand: Reverse

Name: birA [H]

Synonym: LEPBI_I3273

Alternate gene names: 183222618

Gene position: 3387085-3386330 (Counterclockwise)

Preceding gene: 183222621

Following gene: 183222617

Centisome position: 94.09

GC content: 38.62

Gene sequence:

>756_bases
ATGCAATACCGTCTCTTAAAACCTGAATTAGGGCATAGGATCACTTCCGTAAACTCTACCAATGAATGGATCCGAAATCC
AGAAATTCCATTTGGATCGTGGGTGATCGCGGAAGAACAAACGGAAGGAAAAGGTCGGGGACAAAACTCATGGCAATCGT
TAGGTGAAGAGCCATTCATTTTTTCAGGAAAAATCAGGCTTTCTGCAGCAGAAATTTCCTTACCTCTCGTCTCGATATTT
GTGTCTTCAGCCCTTCTCAAAACAATCTATTCATTTTTCCCAAAACGTTTAGATGACACTACGATCAAGTGGCCGAATGA
TATTTACAAAGATGATAAAAAAGTGGCAGGGATATTAATTCAGTCTGAATTTAGTAACGGCGTTTATGACGTTGTGATTG
GAATTGGATTAAATTTTTTTGGGCAAACGATTCCTGAACCATTAGCGGACAAGGCTTCATTTTTATGTGAAAAACAATTA
AGTGAAGGTGAATTGGAAAGATTTGCCAATCAGCTGGTAGTCGAAATCAACCAAGCAGTGATCACTCTCCTCGACCAGAG
CCAAGTATTAAAGGATTTGGTTTGGATCGAAGATCATTCTTTATTAAAAAACAAGGTCATAGAAACTGAGTGGGACCAAA
GAATCGTGAGAGGAAGAGTTTTGGGAATTGATGAATTAGGATTCCTTCTCATTATGACTGAAACTGGTCAAAAGATTGAA
CTGATGGACACGTCACCAAAATTTCGGATAATATAA

Upstream 100 bases:

>100_bases
ACATCCTGTAATTAAAATCGTGTTTCCTGATAATTTCATACATTGAATCTAATCTATTTTTTAGTTTCGTCAATCCGAAT
CATTTAGGAAAAAGACTCAA

Downstream 100 bases:

>100_bases
ATGTCAGAATCACCACTATTACTTGTTATCGACGTTGGGAATACCAATACTGTATTTGGAGTTTTTCGAGAAGGTGAAGA
AACGCCTGATTTTCACAAAA

Product: bifunctional biotin operon repressor/biotin--[acetyl-CoA-carboxylase] synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MQYRLLKPELGHRITSVNSTNEWIRNPEIPFGSWVIAEEQTEGKGRGQNSWQSLGEEPFIFSGKIRLSAAEISLPLVSIF
VSSALLKTIYSFFPKRLDDTTIKWPNDIYKDDKKVAGILIQSEFSNGVYDVVIGIGLNFFGQTIPEPLADKASFLCEKQL
SEGELERFANQLVVEINQAVITLLDQSQVLKDLVWIEDHSLLKNKVIETEWDQRIVRGRVLGIDELGFLLIMTETGQKIE
LMDTSPKFRII

Sequences:

>Translated_251_residues
MQYRLLKPELGHRITSVNSTNEWIRNPEIPFGSWVIAEEQTEGKGRGQNSWQSLGEEPFIFSGKIRLSAAEISLPLVSIF
VSSALLKTIYSFFPKRLDDTTIKWPNDIYKDDKKVAGILIQSEFSNGVYDVVIGIGLNFFGQTIPEPLADKASFLCEKQL
SEGELERFANQLVVEINQAVITLLDQSQVLKDLVWIEDHSLLKNKVIETEWDQRIVRGRVLGIDELGFLLIMTETGQKIE
LMDTSPKFRII
>Mature_251_residues
MQYRLLKPELGHRITSVNSTNEWIRNPEIPFGSWVIAEEQTEGKGRGQNSWQSLGEEPFIFSGKIRLSAAEISLPLVSIF
VSSALLKTIYSFFPKRLDDTTIKWPNDIYKDDKKVAGILIQSEFSNGVYDVVIGIGLNFFGQTIPEPLADKASFLCEKQL
SEGELERFANQLVVEINQAVITLLDQSQVLKDLVWIEDHSLLKNKVIETEWDQRIVRGRVLGIDELGFLLIMTETGQKIE
LMDTSPKFRII

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=221, Percent_Identity=28.0542986425339, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR008988 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28530; Mature: 28530

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYRLLKPELGHRITSVNSTNEWIRNPEIPFGSWVIAEEQTEGKGRGQNSWQSLGEEPFI
CCCEECCHHHCCEEECCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCE
FSGKIRLSAAEISLPLVSIFVSSALLKTIYSFFPKRLDDTTIKWPNDIYKDDKKVAGILI
EECEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHEEEE
QSEFSNGVYDVVIGIGLNFFGQTIPEPLADKASFLCEKQLSEGELERFANQLVVEINQAV
EECCCCCCEEHHHHHCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ITLLDQSQVLKDLVWIEDHSLLKNKVIETEWDQRIVRGRVLGIDELGFLLIMTETGQKIE
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEE
LMDTSPKFRII
EEECCCCEEEC
>Mature Secondary Structure
MQYRLLKPELGHRITSVNSTNEWIRNPEIPFGSWVIAEEQTEGKGRGQNSWQSLGEEPFI
CCCEECCHHHCCEEECCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHCCCCCCE
FSGKIRLSAAEISLPLVSIFVSSALLKTIYSFFPKRLDDTTIKWPNDIYKDDKKVAGILI
EECEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHEEEE
QSEFSNGVYDVVIGIGLNFFGQTIPEPLADKASFLCEKQLSEGELERFANQLVVEINQAV
EECCCCCCEEHHHHHCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ITLLDQSQVLKDLVWIEDHSLLKNKVIETEWDQRIVRGRVLGIDELGFLLIMTETGQKIE
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEE
LMDTSPKFRII
EEECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]