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The map label for this gene is htrB [H]

Identifier: 183222579

GI number: 183222579

Start: 3347303

End: 3348211

Strand: Reverse

Name: htrB [H]

Synonym: LEPBI_I3234

Alternate gene names: 183222579

Gene position: 3348211-3347303 (Counterclockwise)

Preceding gene: 183222580

Following gene: 183222565

Centisome position: 93.01

GC content: 37.62

Gene sequence:

>909_bases
ATGAAATACATCGGATATTTTTTTTCATTTCTCATTGTTTATCTATTTTATTCACCTTTTAAGTTGTTACCCTATCAGTG
GTGTTTGTCCTATGGAATGTTTCTCACTAACTTAATTTATAAGTTTGATAAAAAACATCGAAAGGTTGCGGCCGAGAACA
TTCGATTTGCTTTCCCAGAATATTCGGAAAACCAAATCTTAGATTTGGTGAAAGCACATTATCGACACTTAGGAATTTTA
CTTGCACATACATTGTGGGCGCCTCGCATGACGCGTGCTTGGTTAGATGAATATCTTGTTCTTGATGAAAGTAGTTTAAA
AATAGAAGAAGAAACCAAAAAACAAGGGGTAGGGGTAATTTTAATTTCCGGTCATTTTGGGACTTGGGAAATTTTGGTCC
AATTTTTAGGCATTCGAATGAAGGGTGGGGGAATTTATAAAAAGGTTCGTAATCCATTTGTTGATACCCTTCTGAAACGG
ATGCGATCGAAAAACGGTGTGGTATTGGTTCCTGTAGAAGAATCTACGCAAGTCATCAAACTTCTCAAACAAGGGTATTG
GATTGGATTTGGTGCTGACCAAAATGCAGGAAAAGCAGGGATTTTTGTTCCCTTTATGAATAGGCAAGCTTCCACTTTTG
TGGGACCAGCTCTTATGGCTTATCTCACTGGTGCCAAAATGTTATATTATTCAGTGTTAGCAGGTGAAAACGGGAAAGTG
ATCGTACGTGTGAAAGACTTAGGTTTTGTAGATAAAAAATTATATCCCAAAAAAGACGATGTCATCCGTCATTATACGGA
ACTTTGGACAAAAACTTTAGAAGAGGAAGTGAAGTTATTTCCGGAACAATACTTTTGGGTGCATCGACGATGGCGAACCC
AACCGCAACTAAGTGAGATTCCAAAATAG

Upstream 100 bases:

>100_bases
ACTTTGTTAGTGATATTATGGGAGGATGGGTCATTGGATTAGGCATTGGATTGATCGCAGTCACAATTCATTACTTTTTT
TGGCCCTATTTGGTAACGAT

Downstream 100 bases:

>100_bases
ATTTTTCCTACTTTAGTAAGAGGCATTTTGTAAGGAGATTTTGAAATCGAAATCGAAAGTAGATTTGAATTTTTTGAAAG
ATGTTTTGAAAGTATTTTGA

Product: putative lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKYIGYFFSFLIVYLFYSPFKLLPYQWCLSYGMFLTNLIYKFDKKHRKVAAENIRFAFPEYSENQILDLVKAHYRHLGIL
LAHTLWAPRMTRAWLDEYLVLDESSLKIEEETKKQGVGVILISGHFGTWEILVQFLGIRMKGGGIYKKVRNPFVDTLLKR
MRSKNGVVLVPVEESTQVIKLLKQGYWIGFGADQNAGKAGIFVPFMNRQASTFVGPALMAYLTGAKMLYYSVLAGENGKV
IVRVKDLGFVDKKLYPKKDDVIRHYTELWTKTLEEEVKLFPEQYFWVHRRWRTQPQLSEIPK

Sequences:

>Translated_302_residues
MKYIGYFFSFLIVYLFYSPFKLLPYQWCLSYGMFLTNLIYKFDKKHRKVAAENIRFAFPEYSENQILDLVKAHYRHLGIL
LAHTLWAPRMTRAWLDEYLVLDESSLKIEEETKKQGVGVILISGHFGTWEILVQFLGIRMKGGGIYKKVRNPFVDTLLKR
MRSKNGVVLVPVEESTQVIKLLKQGYWIGFGADQNAGKAGIFVPFMNRQASTFVGPALMAYLTGAKMLYYSVLAGENGKV
IVRVKDLGFVDKKLYPKKDDVIRHYTELWTKTLEEEVKLFPEQYFWVHRRWRTQPQLSEIPK
>Mature_302_residues
MKYIGYFFSFLIVYLFYSPFKLLPYQWCLSYGMFLTNLIYKFDKKHRKVAAENIRFAFPEYSENQILDLVKAHYRHLGIL
LAHTLWAPRMTRAWLDEYLVLDESSLKIEEETKKQGVGVILISGHFGTWEILVQFLGIRMKGGGIYKKVRNPFVDTLLKR
MRSKNGVVLVPVEESTQVIKLLKQGYWIGFGADQNAGKAGIFVPFMNRQASTFVGPALMAYLTGAKMLYYSVLAGENGKV
IVRVKDLGFVDKKLYPKKDDVIRHYTELWTKTLEEEVKLFPEQYFWVHRRWRTQPQLSEIPK

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=279, Percent_Identity=27.5985663082437, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87082094, Length=285, Percent_Identity=25.2631578947368, Blast_Score=95, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35306; Mature: 35306

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIGYFFSFLIVYLFYSPFKLLPYQWCLSYGMFLTNLIYKFDKKHRKVAAENIRFAFPE
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
YSENQILDLVKAHYRHLGILLAHTLWAPRMTRAWLDEYLVLDESSLKIEEETKKQGVGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEECCCCCCCCHHHHHCCCEEE
LISGHFGTWEILVQFLGIRMKGGGIYKKVRNPFVDTLLKRMRSKNGVVLVPVEESTQVIK
EEECCCCHHHHHHHHHCCEECCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEECCHHHHHH
LLKQGYWIGFGADQNAGKAGIFVPFMNRQASTFVGPALMAYLTGAKMLYYSVLAGENGKV
HHHCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IVRVKDLGFVDKKLYPKKDDVIRHYTELWTKTLEEEVKLFPEQYFWVHRRWRTQPQLSEI
EEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCC
PK
CC
>Mature Secondary Structure
MKYIGYFFSFLIVYLFYSPFKLLPYQWCLSYGMFLTNLIYKFDKKHRKVAAENIRFAFPE
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCC
YSENQILDLVKAHYRHLGILLAHTLWAPRMTRAWLDEYLVLDESSLKIEEETKKQGVGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHEECCCCCCCCHHHHHCCCEEE
LISGHFGTWEILVQFLGIRMKGGGIYKKVRNPFVDTLLKRMRSKNGVVLVPVEESTQVIK
EEECCCCHHHHHHHHHCCEECCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEECCHHHHHH
LLKQGYWIGFGADQNAGKAGIFVPFMNRQASTFVGPALMAYLTGAKMLYYSVLAGENGKV
HHHCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IVRVKDLGFVDKKLYPKKDDVIRHYTELWTKTLEEEVKLFPEQYFWVHRRWRTQPQLSEI
EEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCC
PK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]