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The map label for this gene is aat [H]

Identifier: 183222564

GI number: 183222564

Start: 3332950

End: 3333615

Strand: Reverse

Name: aat [H]

Synonym: LEPBI_I3219

Alternate gene names: 183222564

Gene position: 3333615-3332950 (Counterclockwise)

Preceding gene: 183222565

Following gene: 183222561

Centisome position: 92.61

GC content: 42.94

Gene sequence:

>666_bases
TTGACAAAGAGGAGTTTTGAGCAATTTTTTAAAAACCCTCGGTTGTGGAGAGAGGATTTAGTCGCAGTTGGGGGAGATTT
TTCCGTTGATCGTTTGTTATACGCATACACCCACGGAATTTTCCCCTGGTCAGAAGATCCGATTCGTTGGTATTCTTTAG
ACCCACGTGCCATTTTTGATATCAATCGAGTTCATTTTTCAAAAACAGTTTTAAGAAAGGTCCGACAAAAAAAATTCCGA
ATTACGTTCAACGAAGCCTTTGCCATTGTCATGCAAGCCTGCGCTTACCGTGAAAAGGACAATACCTGGATCACTCCTGG
TTTTATCGATGGGTATTTAGAGCTCCACAAACAAGGTTGGGCTCACTCCGTAGAAGTTTGGAATTCTGAGAATATCTTAG
TGGGTGGTGTTTACGGAGTGGCCATCGGGAAATTTTTTGCAGGGGAAAGTATGTTTTCCTTTGAGTCGGATGCGGGGAAA
ATTGCTCTGTATCATTTGTTCGCACGACTACGAGAATCCAACTTTGTTTTATTTGACACCCAACAACTCAATCATGTGAC
CTGGCAATTGGGTGCCTATGAAATTCCCAAATTATCGTATTTGGACCGATTGGAACGAGCCGTCGTAGATATTGTCCCTT
GGGTCATTCCACCTTCACACGACTGA

Upstream 100 bases:

>100_bases
GGATATTACAATTTGATGGCCTATCCAATATCAATGATGAGAATGGGAAAAGTTTAAAAGTAAAAATCGTTTACGATTAT
CCGAAGGAAGTTTTGGAACC

Downstream 100 bases:

>100_bases
TCTCATCCAATTCGACCTTCCAAAGCGATTGAATTTCTTCCGTAACAGACACCCATTCTTTATCACCTAACTTCGGTGCC
TTGTAAGGATCAAAATTGGG

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTKRSFEQFFKNPRLWREDLVAVGGDFSVDRLLYAYTHGIFPWSEDPIRWYSLDPRAIFDINRVHFSKTVLRKVRQKKFR
ITFNEAFAIVMQACAYREKDNTWITPGFIDGYLELHKQGWAHSVEVWNSENILVGGVYGVAIGKFFAGESMFSFESDAGK
IALYHLFARLRESNFVLFDTQQLNHVTWQLGAYEIPKLSYLDRLERAVVDIVPWVIPPSHD

Sequences:

>Translated_221_residues
MTKRSFEQFFKNPRLWREDLVAVGGDFSVDRLLYAYTHGIFPWSEDPIRWYSLDPRAIFDINRVHFSKTVLRKVRQKKFR
ITFNEAFAIVMQACAYREKDNTWITPGFIDGYLELHKQGWAHSVEVWNSENILVGGVYGVAIGKFFAGESMFSFESDAGK
IALYHLFARLRESNFVLFDTQQLNHVTWQLGAYEIPKLSYLDRLERAVVDIVPWVIPPSHD
>Mature_220_residues
TKRSFEQFFKNPRLWREDLVAVGGDFSVDRLLYAYTHGIFPWSEDPIRWYSLDPRAIFDINRVHFSKTVLRKVRQKKFRI
TFNEAFAIVMQACAYREKDNTWITPGFIDGYLELHKQGWAHSVEVWNSENILVGGVYGVAIGKFFAGESMFSFESDAGKI
ALYHLFARLRESNFVLFDTQQLNHVTWQLGAYEIPKLSYLDRLERAVVDIVPWVIPPSHD

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine [H]

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family [H]

Homologues:

Organism=Escherichia coli, GI1787111, Length=196, Percent_Identity=44.8979591836735, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR004616 [H]

Pfam domain/function: PF03588 Leu_Phe_trans [H]

EC number: =2.3.2.6 [H]

Molecular weight: Translated: 25848; Mature: 25717

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRSFEQFFKNPRLWREDLVAVGGDFSVDRLLYAYTHGIFPWSEDPIRWYSLDPRAIFD
CCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHEEE
INRVHFSKTVLRKVRQKKFRITFNEAFAIVMQACAYREKDNTWITPGFIDGYLELHKQGW
HHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHCCC
AHSVEVWNSENILVGGVYGVAIGKFFAGESMFSFESDAGKIALYHLFARLRESNFVLFDT
CEEEEEECCCCEEEECHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEEC
QQLNHVTWQLGAYEIPKLSYLDRLERAVVDIVPWVIPPSHD
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TKRSFEQFFKNPRLWREDLVAVGGDFSVDRLLYAYTHGIFPWSEDPIRWYSLDPRAIFD
CHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHEEE
INRVHFSKTVLRKVRQKKFRITFNEAFAIVMQACAYREKDNTWITPGFIDGYLELHKQGW
HHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHCCC
AHSVEVWNSENILVGGVYGVAIGKFFAGESMFSFESDAGKIALYHLFARLRESNFVLFDT
CEEEEEECCCCEEEECHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEEC
QQLNHVTWQLGAYEIPKLSYLDRLERAVVDIVPWVIPPSHD
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA