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The map label for this gene is fadE25 [H]

Identifier: 183222449

GI number: 183222449

Start: 3218554

End: 3220266

Strand: Direct

Name: fadE25 [H]

Synonym: LEPBI_I3103

Alternate gene names: 183222449

Gene position: 3218554-3220266 (Clockwise)

Preceding gene: 183222447

Following gene: 183222452

Centisome position: 89.41

GC content: 38.12

Gene sequence:

>1713_bases
ATGATCCAAAACAATTATTTTCTTAGCAACGAAGACCTGAAAGAACACTTCTATGAATTAATCGATTGGAATGAAATTGT
CCCTATTTACGAGAATCAGTTTTTAGACGCAAAACTTTTTGAATCAACAAAAAATCCCAAACTAGAAATGGCACCAACTA
ACGTTGATGAAGCGATTTCATTTTACGAAGAGATATTGAAATCCTGTGGTGAAATCAGCGGAATGTATGTTTCTCAAGTG
GCCTCTATCGTCGATTCTAAAGGATTAAAATTTGAAAATGGTGAAGTGATCCATCCAAAAGAAATGGTAGATGTGATTCA
AATGTATTATGATGCAGGACTCGGTCCAGCCGCCTTCAAACGAAAATACGGTGGTTTAGGTGTACCAAGTATCATCAAAG
CAATGATAGCAGAGATTATGTATAGGTCAGATAGCTCGATCACAATTGCCGTTGGAAGTATGGGTCTTGCTGCAATCTTG
GAAGTGTGTGCATCGGAAGAAATGAAAAACGAATGGATCCCGAAACTCATCTCAGGTAATTATACAGTCACAATGGGATT
ATCTGAACCAGACTTCGGATCTGACTTACCAAACATTACCACGAAAGCTTTCAAAAATGGAGAAGAATGGTTTTTAAATG
GAACCAAACGATTTCAAACAGTTGCATGCGGGGTCAATGGAGGATCTGGCATCACTCTCACACTTGCAAGAACTGGAACA
CAAGAAAGTGGAGCAAGAGGTCTTTCGTTCTTTATCGTTGAAAACAAAGATTATGTTGTACAAGGTATTGAAAAAAAACT
AGGAATCAAAGCATCTGCTACATGCGAAACAGTCTTTGAAAATAGCAAAGGTCATTTGGTTGGAAAGGAAGGATTTGGTC
TTGTAAAATACGTAATGGGTATGTTGAATGGTGCGCGTCTCAGCGTGTCTAGCCAAGGTACCGGAATTGTTACCGCTGCA
TTTGAAGAATCATACAAATATGCGAATGAAAGAATTCAATTTGGAAAACCAATTTATGAAATCCCTGCTGTTCGTAGAAT
GTTAGATCGAATGGAAAGAGAACTTGCTGGAATGCGTTGCCTAATGGTAGAAGCTGCCTATTCAGTGGATAAATATTACT
GGTATGAAGATGGTAGAGAAGTCTCTACAGAAGAACTTAAATCGGCAAAATTTTGGGAGAAAGTTGCAAATACGCTCACT
CCAATTTCCAAGTATTACAATTCAGAAATGTGTAATGATTTAGTTTACGATGGTTTACAGGTATTAGGTGGCGCTGGTTA
TACGGAGGACTATGATCTTTCCCGATTGTATCGTGACGCACGGATCACAAATATTTATGATGGCACCACACAAATCCAAG
TCAACGCGGCCATTGGAGGAATCACATCTGGAATGAGTGGAACGGGAACCTTTCGAGCTTACTTAGACCACTTGTCTCTA
GGATCTGATGGAAACAAACGACTAAAAGAAATCCGTGAAGTATTTGAATCCATCGTGGAATCATTTAAGTCCATTCAAGA
TCAAAGCACAAAAGAAGCATATAGTTTCGAAGTGGTAGAATCAGCTGCAAGAGTGATCATCGGTTATCTTATGGAACGAA
ACAAAAACAAATCTAAATCGAGAAAGGAAATTCGAACCAAGTGGTGTAAAGAATTTCATAACGATAGTTTTGCAATTCTC
AGTTCCAATTTAATCAAATTAAAATCCTTATAA

Upstream 100 bases:

>100_bases
TCGGTTCACTTTGGATTCAATTTATTATATTTTTTGATGAATCTATTTTTTTTAATCGCAAGACAACGCTTGGTATTATC
TTTTTAACCTGAGGACAAAA

Downstream 100 bases:

>100_bases
AAAAAAAGGGTCCTCCGTAAAGAGGACCCACGAGAAAGTAAAGGGAAGGACTTCCTCCATTGCGAGGTAAAGGAAGTCCG
AGATCGTTCAGATTAAGTGA

Product: acyl-CoA dehydrogenase

Products: 2,3-dehydroacyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MIQNNYFLSNEDLKEHFYELIDWNEIVPIYENQFLDAKLFESTKNPKLEMAPTNVDEAISFYEEILKSCGEISGMYVSQV
ASIVDSKGLKFENGEVIHPKEMVDVIQMYYDAGLGPAAFKRKYGGLGVPSIIKAMIAEIMYRSDSSITIAVGSMGLAAIL
EVCASEEMKNEWIPKLISGNYTVTMGLSEPDFGSDLPNITTKAFKNGEEWFLNGTKRFQTVACGVNGGSGITLTLARTGT
QESGARGLSFFIVENKDYVVQGIEKKLGIKASATCETVFENSKGHLVGKEGFGLVKYVMGMLNGARLSVSSQGTGIVTAA
FEESYKYANERIQFGKPIYEIPAVRRMLDRMERELAGMRCLMVEAAYSVDKYYWYEDGREVSTEELKSAKFWEKVANTLT
PISKYYNSEMCNDLVYDGLQVLGGAGYTEDYDLSRLYRDARITNIYDGTTQIQVNAAIGGITSGMSGTGTFRAYLDHLSL
GSDGNKRLKEIREVFESIVESFKSIQDQSTKEAYSFEVVESAARVIIGYLMERNKNKSKSRKEIRTKWCKEFHNDSFAIL
SSNLIKLKSL

Sequences:

>Translated_570_residues
MIQNNYFLSNEDLKEHFYELIDWNEIVPIYENQFLDAKLFESTKNPKLEMAPTNVDEAISFYEEILKSCGEISGMYVSQV
ASIVDSKGLKFENGEVIHPKEMVDVIQMYYDAGLGPAAFKRKYGGLGVPSIIKAMIAEIMYRSDSSITIAVGSMGLAAIL
EVCASEEMKNEWIPKLISGNYTVTMGLSEPDFGSDLPNITTKAFKNGEEWFLNGTKRFQTVACGVNGGSGITLTLARTGT
QESGARGLSFFIVENKDYVVQGIEKKLGIKASATCETVFENSKGHLVGKEGFGLVKYVMGMLNGARLSVSSQGTGIVTAA
FEESYKYANERIQFGKPIYEIPAVRRMLDRMERELAGMRCLMVEAAYSVDKYYWYEDGREVSTEELKSAKFWEKVANTLT
PISKYYNSEMCNDLVYDGLQVLGGAGYTEDYDLSRLYRDARITNIYDGTTQIQVNAAIGGITSGMSGTGTFRAYLDHLSL
GSDGNKRLKEIREVFESIVESFKSIQDQSTKEAYSFEVVESAARVIIGYLMERNKNKSKSRKEIRTKWCKEFHNDSFAIL
SSNLIKLKSL
>Mature_570_residues
MIQNNYFLSNEDLKEHFYELIDWNEIVPIYENQFLDAKLFESTKNPKLEMAPTNVDEAISFYEEILKSCGEISGMYVSQV
ASIVDSKGLKFENGEVIHPKEMVDVIQMYYDAGLGPAAFKRKYGGLGVPSIIKAMIAEIMYRSDSSITIAVGSMGLAAIL
EVCASEEMKNEWIPKLISGNYTVTMGLSEPDFGSDLPNITTKAFKNGEEWFLNGTKRFQTVACGVNGGSGITLTLARTGT
QESGARGLSFFIVENKDYVVQGIEKKLGIKASATCETVFENSKGHLVGKEGFGLVKYVMGMLNGARLSVSSQGTGIVTAA
FEESYKYANERIQFGKPIYEIPAVRRMLDRMERELAGMRCLMVEAAYSVDKYYWYEDGREVSTEELKSAKFWEKVANTLT
PISKYYNSEMCNDLVYDGLQVLGGAGYTEDYDLSRLYRDARITNIYDGTTQIQVNAAIGGITSGMSGTGTFRAYLDHLSL
GSDGNKRLKEIREVFESIVESFKSIQDQSTKEAYSFEVVESAARVIIGYLMERNKNKSKSRKEIRTKWCKEFHNDSFAIL
SSNLIKLKSL

Specific function: Unknown

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=358, Percent_Identity=28.4916201117318, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI21361497, Length=384, Percent_Identity=31.25, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI4557233, Length=402, Percent_Identity=28.6069651741293, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI4501857, Length=300, Percent_Identity=30.3333333333333, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI187960098, Length=363, Percent_Identity=30.0275482093664, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI4557231, Length=363, Percent_Identity=30.0275482093664, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI7656849, Length=360, Percent_Identity=27.5, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI226958412, Length=341, Percent_Identity=26.9794721407625, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI226958414, Length=341, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI4557235, Length=345, Percent_Identity=27.8260869565217, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI76496475, Length=347, Percent_Identity=27.6657060518732, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI4503943, Length=365, Percent_Identity=25.2054794520548, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI7669494, Length=346, Percent_Identity=24.5664739884393, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI87081958, Length=371, Percent_Identity=22.911051212938, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87082384, Length=305, Percent_Identity=27.8688524590164, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786223, Length=271, Percent_Identity=24.3542435424354, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17508101, Length=376, Percent_Identity=27.6595744680851, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17534899, Length=359, Percent_Identity=29.5264623955432, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506239, Length=360, Percent_Identity=28.8888888888889, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538396, Length=350, Percent_Identity=28.8571428571429, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17570075, Length=358, Percent_Identity=28.2122905027933, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17569725, Length=359, Percent_Identity=27.5766016713092, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17533517, Length=357, Percent_Identity=26.3305322128852, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71985184, Length=333, Percent_Identity=29.1291291291291, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71990804, Length=273, Percent_Identity=28.2051282051282, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI32563615, Length=232, Percent_Identity=29.3103448275862, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI86563381, Length=366, Percent_Identity=26.5027322404372, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI86563383, Length=366, Percent_Identity=26.5027322404372, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985192, Length=252, Percent_Identity=30.1587301587302, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505929, Length=360, Percent_Identity=25, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982178, Length=241, Percent_Identity=29.8755186721992, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17534353, Length=355, Percent_Identity=23.943661971831, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21356377, Length=400, Percent_Identity=30.25, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24666513, Length=358, Percent_Identity=27.0949720670391, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646207, Length=407, Percent_Identity=27.2727272727273, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24660351, Length=377, Percent_Identity=28.6472148541114, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281363737, Length=349, Percent_Identity=29.2263610315186, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355753, Length=340, Percent_Identity=27.9411764705882, Blast_Score=112, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.3

Molecular weight: Translated: 63702; Mature: 63702

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNNYFLSNEDLKEHFYELIDWNEIVPIYENQFLDAKLFESTKNPKLEMAPTNVDEAIS
CCCCCEEECCCHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHCCCCCCEEECCCCHHHHHH
FYEEILKSCGEISGMYVSQVASIVDSKGLKFENGEVIHPKEMVDVIQMYYDAGLGPAAFK
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCEECHHHHHHHHHHHHHCCCCHHHHH
RKYGGLGVPSIIKAMIAEIMYRSDSSITIAVGSMGLAAILEVCASEEMKNEWIPKLISGN
HHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHCCC
YTVTMGLSEPDFGSDLPNITTKAFKNGEEWFLNGTKRFQTVACGVNGGSGITLTLARTGT
EEEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCCEEEEEEEECCCCCCEEEEEEECCC
QESGARGLSFFIVENKDYVVQGIEKKLGIKASATCETVFENSKGHLVGKEGFGLVKYVMG
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCEECCCCCCHHHHHHH
MLNGARLSVSSQGTGIVTAAFEESYKYANERIQFGKPIYEIPAVRRMLDRMERELAGMRC
HCCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LMVEAAYSVDKYYWYEDGREVSTEELKSAKFWEKVANTLTPISKYYNSEMCNDLVYDGLQ
HHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGGAGYTEDYDLSRLYRDARITNIYDGTTQIQVNAAIGGITSGMSGTGTFRAYLDHLSL
HHCCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCC
GSDGNKRLKEIREVFESIVESFKSIQDQSTKEAYSFEVVESAARVIIGYLMERNKNKSKS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RKEIRTKWCKEFHNDSFAILSSNLIKLKSL
HHHHHHHHHHHHCCCCEEEHHCCCEEECCC
>Mature Secondary Structure
MIQNNYFLSNEDLKEHFYELIDWNEIVPIYENQFLDAKLFESTKNPKLEMAPTNVDEAIS
CCCCCEEECCCHHHHHHHHHHCCCCCCCHHCCCCCCHHHHHCCCCCCEEECCCCHHHHHH
FYEEILKSCGEISGMYVSQVASIVDSKGLKFENGEVIHPKEMVDVIQMYYDAGLGPAAFK
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCEECHHHHHHHHHHHHHCCCCHHHHH
RKYGGLGVPSIIKAMIAEIMYRSDSSITIAVGSMGLAAILEVCASEEMKNEWIPKLISGN
HHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHCCC
YTVTMGLSEPDFGSDLPNITTKAFKNGEEWFLNGTKRFQTVACGVNGGSGITLTLARTGT
EEEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCCEEEEEEEECCCCCCEEEEEEECCC
QESGARGLSFFIVENKDYVVQGIEKKLGIKASATCETVFENSKGHLVGKEGFGLVKYVMG
CCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCEECCCCCCHHHHHHH
MLNGARLSVSSQGTGIVTAAFEESYKYANERIQFGKPIYEIPAVRRMLDRMERELAGMRC
HCCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LMVEAAYSVDKYYWYEDGREVSTEELKSAKFWEKVANTLTPISKYYNSEMCNDLVYDGLQ
HHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLGGAGYTEDYDLSRLYRDARITNIYDGTTQIQVNAAIGGITSGMSGTGTFRAYLDHLSL
HHCCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCC
GSDGNKRLKEIREVFESIVESFKSIQDQSTKEAYSFEVVESAARVIIGYLMERNKNKSKS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RKEIRTKWCKEFHNDSFAILSSNLIKLKSL
HHHHHHHHHHHHCCCCEEEHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acceptor

Specific reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]