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The map label for this gene is cca [H]

Identifier: 183222200

GI number: 183222200

Start: 2939708

End: 2941000

Strand: Direct

Name: cca [H]

Synonym: LEPBI_I2850

Alternate gene names: 183222200

Gene position: 2939708-2941000 (Clockwise)

Preceding gene: 183222199

Following gene: 183222201

Centisome position: 81.67

GC content: 33.57

Gene sequence:

>1293_bases
TTGCCAATAGACCAGAGCCTCACTACTTTAATCCCCACTTTACATAAAAAACATTTAGATACGATCAATTTTGCATTAAA
AAATGCAGGATTTGAATGTTATCTCGTAGGTGGATCGGTTCGAGATTTAGTGATGAAAAAAATTCCCAAAGAATATGATC
TCACGACAAATGCAGAACCTAAAGATGTTAAAAAATTATTTCGCACTGTCATTGATACTGGAATCGAACATGGCACAGTC
ACAGTCGTATTAGACAAAGTAAATTATGAAGTGACCACTTATCGAATCGACAAAGATTACGTGGATGGTCGAAGGCCTGG
TCATGTTGAGTTTGGAACAACTTTACATGAAGATTTAAAGAGAAGGGATTTTACAATGAATGCCTTGGCTTATGATCTAA
CTTCAGGTGTGTTGATTGATGATCATAATGGACTCGCCGATATCAAAAGCAAAACCATTCGAACTATAGGGAATCCAATA
CAAAGGTTTACAGAGGATGGACTTAGGCCAATTAGAGCCTTAAGATTTGCTAGTACTCTAAATTTTAAGATCGATGAAGA
AACAAAAAAAGCAATTCATCAAACAAAACATATCACAAAAAAAATTTCCTTAGAAAGATTTCAGGATGAAATATTGAAAT
CATTCCTTGGCGAAAAACCATCAGTTATGATTGCACTTTTAGTCGAAGAAAACCTATTTCAAGTTTTTCTTCCTTCTATG
CAGCTTGATTTGATTCCAAATGAAATCTTTTTGGAAGAATTGAATAAGGTTTCAAAAGATTTAGTAGGGTTGCAACTTTC
CTTTGCATTTTATTCAATTTTACCGAATGTAAGTTTAAAGGAATTGGAAACATATTTAAGATCCCTCAAATTTTCTGGAC
AGAATATAAAGGATACCCTTCTCTTCTCTGAACTCATTCAAAAATGGAATCAATACCCTGATTTAAATTCTCTCACAACT
TACGATATTAAAAAATATTTCTTAGCGCCCGTTAAACGGCATTTCCAATCGCGATATGAATTGAACGAAATCTTTTTTCA
AAAACTCACGCCGATTTTCGGAAATGAAATTATGAAATTACTTTCAATTTGGAATGAAAATCCTCCACTTCTGTTGTCCG
ATTTATCCATCAATGGGAATCATTTGAGTGAAAATTTCCCAGATTTGCCCAAACTCAAATTTGGAGAGGTCTTAAGTTTC
CTTTTAGAACTGGTTCTGCAGTCGCCTAAAGAAAATGAATTTCACAAATTAATCACCCATTCTGCTGAATTTATAAACAA
TTTGACCAAATAA

Upstream 100 bases:

>100_bases
AATGCATTGACGATCCTTGGAATGGGATGCCTTCTTCAAAACAACTCAAAGGTTTCACAACTGAATCTGTTGAAATCGTT
TTTAGAGGCAAGTGTAGAAA

Downstream 100 bases:

>100_bases
AAAAATTTCAGTAACGATTGCTACGGACATTTTGTTCTCTTTTTAATCAATTTTCATCTTTTTTTTCTTTTTTTTCCCTG
TGAAACTCCCAAAGTACTAA

Product: putative tRNA nucleotidyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPIDQSLTTLIPTLHKKHLDTINFALKNAGFECYLVGGSVRDLVMKKIPKEYDLTTNAEPKDVKKLFRTVIDTGIEHGTV
TVVLDKVNYEVTTYRIDKDYVDGRRPGHVEFGTTLHEDLKRRDFTMNALAYDLTSGVLIDDHNGLADIKSKTIRTIGNPI
QRFTEDGLRPIRALRFASTLNFKIDEETKKAIHQTKHITKKISLERFQDEILKSFLGEKPSVMIALLVEENLFQVFLPSM
QLDLIPNEIFLEELNKVSKDLVGLQLSFAFYSILPNVSLKELETYLRSLKFSGQNIKDTLLFSELIQKWNQYPDLNSLTT
YDIKKYFLAPVKRHFQSRYELNEIFFQKLTPIFGNEIMKLLSIWNENPPLLLSDLSINGNHLSENFPDLPKLKFGEVLSF
LLELVLQSPKENEFHKLITHSAEFINNLTK

Sequences:

>Translated_430_residues
MPIDQSLTTLIPTLHKKHLDTINFALKNAGFECYLVGGSVRDLVMKKIPKEYDLTTNAEPKDVKKLFRTVIDTGIEHGTV
TVVLDKVNYEVTTYRIDKDYVDGRRPGHVEFGTTLHEDLKRRDFTMNALAYDLTSGVLIDDHNGLADIKSKTIRTIGNPI
QRFTEDGLRPIRALRFASTLNFKIDEETKKAIHQTKHITKKISLERFQDEILKSFLGEKPSVMIALLVEENLFQVFLPSM
QLDLIPNEIFLEELNKVSKDLVGLQLSFAFYSILPNVSLKELETYLRSLKFSGQNIKDTLLFSELIQKWNQYPDLNSLTT
YDIKKYFLAPVKRHFQSRYELNEIFFQKLTPIFGNEIMKLLSIWNENPPLLLSDLSINGNHLSENFPDLPKLKFGEVLSF
LLELVLQSPKENEFHKLITHSAEFINNLTK
>Mature_429_residues
PIDQSLTTLIPTLHKKHLDTINFALKNAGFECYLVGGSVRDLVMKKIPKEYDLTTNAEPKDVKKLFRTVIDTGIEHGTVT
VVLDKVNYEVTTYRIDKDYVDGRRPGHVEFGTTLHEDLKRRDFTMNALAYDLTSGVLIDDHNGLADIKSKTIRTIGNPIQ
RFTEDGLRPIRALRFASTLNFKIDEETKKAIHQTKHITKKISLERFQDEILKSFLGEKPSVMIALLVEENLFQVFLPSMQ
LDLIPNEIFLEELNKVSKDLVGLQLSFAFYSILPNVSLKELETYLRSLKFSGQNIKDTLLFSELIQKWNQYPDLNSLTTY
DIKKYFLAPVKRHFQSRYELNEIFFQKLTPIFGNEIMKLLSIWNENPPLLLSDLSINGNHLSENFPDLPKLKFGEVLSFL
LELVLQSPKENEFHKLITHSAEFINNLTK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=243, Percent_Identity=36.2139917695473, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI87081691, Length=236, Percent_Identity=35.1694915254237, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1789436, Length=219, Percent_Identity=29.6803652968037, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=242, Percent_Identity=34.297520661157, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321016, Length=232, Percent_Identity=30.1724137931034, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281360112, Length=248, Percent_Identity=33.0645161290323, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357337, Length=248, Percent_Identity=33.0645161290323, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 49566; Mature: 49435

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDQSLTTLIPTLHKKHLDTINFALKNAGFECYLVGGSVRDLVMKKIPKEYDLTTNAEP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCH
KDVKKLFRTVIDTGIEHGTVTVVLDKVNYEVTTYRIDKDYVDGRRPGHVEFGTTLHEDLK
HHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECHHHCCCCCCCCEECCHHHHHHHH
RRDFTMNALAYDLTSGVLIDDHNGLADIKSKTIRTIGNPIQRFTEDGLRPIRALRFASTL
HCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NFKIDEETKKAIHQTKHITKKISLERFQDEILKSFLGEKPSVMIALLVEENLFQVFLPSM
CCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCH
QLDLIPNEIFLEELNKVSKDLVGLQLSFAFYSILPNVSLKELETYLRSLKFSGQNIKDTL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHH
LFSELIQKWNQYPDLNSLTTYDIKKYFLAPVKRHFQSRYELNEIFFQKLTPIFGNEIMKL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LSIWNENPPLLLSDLSINGNHLSENFPDLPKLKFGEVLSFLLELVLQSPKENEFHKLITH
HHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
SAEFINNLTK
HHHHHHHCCC
>Mature Secondary Structure 
PIDQSLTTLIPTLHKKHLDTINFALKNAGFECYLVGGSVRDLVMKKIPKEYDLTTNAEP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCH
KDVKKLFRTVIDTGIEHGTVTVVLDKVNYEVTTYRIDKDYVDGRRPGHVEFGTTLHEDLK
HHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECHHHCCCCCCCCEECCHHHHHHHH
RRDFTMNALAYDLTSGVLIDDHNGLADIKSKTIRTIGNPIQRFTEDGLRPIRALRFASTL
HCCCHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NFKIDEETKKAIHQTKHITKKISLERFQDEILKSFLGEKPSVMIALLVEENLFQVFLPSM
CCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCH
QLDLIPNEIFLEELNKVSKDLVGLQLSFAFYSILPNVSLKELETYLRSLKFSGQNIKDTL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHH
LFSELIQKWNQYPDLNSLTTYDIKKYFLAPVKRHFQSRYELNEIFFQKLTPIFGNEIMKL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LSIWNENPPLLLSDLSINGNHLSENFPDLPKLKFGEVLSFLLELVLQSPKENEFHKLITH
HHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
SAEFINNLTK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA