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The map label for this gene is blh [H]

Identifier: 183221989

GI number: 183221989

Start: 2722656

End: 2723372

Strand: Direct

Name: blh [H]

Synonym: LEPBI_I2630

Alternate gene names: 183221989

Gene position: 2722656-2723372 (Clockwise)

Preceding gene: 183221987

Following gene: 183221990

Centisome position: 75.64

GC content: 38.63

Gene sequence:

>717_bases
ATGGTAGATACAGATTCGATGAATGGAATTCAGATAAAACCTCTATATGACTTTGCCTCAGGGACATGGACTTACTTACT
TTTAGAGATTGAATCAAAACAAGCAGTACTCATAGATCCTGTGATCGAAAATCTTGAAAGAGACTTACAACTACTCGAAT
CTATGGAGTTTCAATTGGTAGCGACCATTGAAACACATATGCATGCTGATCACATTACAGCGGCTGGTAATTTGAGGGAA
AAAACAGGATGTGACTCCTATGCCCCCGAACACTCTGGAGCAACCTGTGCGACTCATTTTTTAAAAGATAATGATGAGAT
TGTGATTGGAAAATTAAATATGAAAGTGATCCACACGCCAGGTCATACACCTTGTTCCATTTCGTTATTTTTAAATGGAA
GGTATGTTTTCACCGGAGATGCTTTATTTGTTAGAGGTTGTGGTAGAACTGATTTCCAGGGAGGGAGTGCAGAATCCTTA
TACCATTCCATTACAGGCAAACTTTTTTTATTACCAGATGAAACCATTGTATATCCTGGGCATGATTATAAAGGATTTGT
TTCTTCTACCATCGGCGAAGAAAAAAAGTGGAACCCTCGTATCGCCAATCGATCACTAGAAGAATTTCAATCCATCATGG
ACAATTTAAACTTACCGGAACCTAAAAAAATTCATGAGGCAGTGCCTGCCAATCGTGCTTGTGGGAAAACATTATGA

Upstream 100 bases:

>100_bases
AAAGTGTATACCATAGGGGGTATGGTATACAGACCATTCCCTTACCATTCGAAAGGAAAAATAATGTTGAATGGTGAAAA
AAATTTAGGAGAATGATACC

Downstream 100 bases:

>100_bases
TGATAGGAATTTTATTTTTAGTATTAGGCATTGGCGCTGGAATTTTAGGTGGCCTTGTTGGAATTGGCGGTGGGATCTTA
CTCGTCCCCGCATTAGTTTA

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MVDTDSMNGIQIKPLYDFASGTWTYLLLEIESKQAVLIDPVIENLERDLQLLESMEFQLVATIETHMHADHITAAGNLRE
KTGCDSYAPEHSGATCATHFLKDNDEIVIGKLNMKVIHTPGHTPCSISLFLNGRYVFTGDALFVRGCGRTDFQGGSAESL
YHSITGKLFLLPDETIVYPGHDYKGFVSSTIGEEKKWNPRIANRSLEEFQSIMDNLNLPEPKKIHEAVPANRACGKTL

Sequences:

>Translated_238_residues
MVDTDSMNGIQIKPLYDFASGTWTYLLLEIESKQAVLIDPVIENLERDLQLLESMEFQLVATIETHMHADHITAAGNLRE
KTGCDSYAPEHSGATCATHFLKDNDEIVIGKLNMKVIHTPGHTPCSISLFLNGRYVFTGDALFVRGCGRTDFQGGSAESL
YHSITGKLFLLPDETIVYPGHDYKGFVSSTIGEEKKWNPRIANRSLEEFQSIMDNLNLPEPKKIHEAVPANRACGKTL
>Mature_238_residues
MVDTDSMNGIQIKPLYDFASGTWTYLLLEIESKQAVLIDPVIENLERDLQLLESMEFQLVATIETHMHADHITAAGNLRE
KTGCDSYAPEHSGATCATHFLKDNDEIVIGKLNMKVIHTPGHTPCSISLFLNGRYVFTGDALFVRGCGRTDFQGGSAESL
YHSITGKLFLLPDETIVYPGHDYKGFVSSTIGEEKKWNPRIANRSLEEFQSIMDNLNLPEPKKIHEAVPANRACGKTL

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-B beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=228, Percent_Identity=48.2456140350877, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI116642887, Length=233, Percent_Identity=30.0429184549356, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI21703352, Length=233, Percent_Identity=30.0429184549356, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI94538320, Length=171, Percent_Identity=32.7485380116959, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI94538322, Length=171, Percent_Identity=32.7485380116959, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI14150041, Length=228, Percent_Identity=27.6315789473684, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI46361987, Length=170, Percent_Identity=31.7647058823529, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787158, Length=194, Percent_Identity=29.3814432989691, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1786406, Length=158, Percent_Identity=34.1772151898734, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538952, Length=231, Percent_Identity=39.8268398268398, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17536925, Length=247, Percent_Identity=27.5303643724696, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=225, Percent_Identity=28.8888888888889, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221330176, Length=225, Percent_Identity=48, Blast_Score=217, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24667703, Length=237, Percent_Identity=30.379746835443, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356335, Length=237, Percent_Identity=30.379746835443, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667711, Length=237, Percent_Identity=30.379746835443, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005939
- InterPro:   IPR001279 [H]

Pfam domain/function: PF04273 DUF442; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 26470; Mature: 26470

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDTDSMNGIQIKPLYDFASGTWTYLLLEIESKQAVLIDPVIENLERDLQLLESMEFQLV
CCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCEEEE
ATIETHMHADHITAAGNLREKTGCDSYAPEHSGATCATHFLKDNDEIVIGKLNMKVIHTP
EEEEHHHCCCCEEECCCCCHHCCCCCCCCCCCCCCEEEHEECCCCCEEEEEEEEEEEECC
GHTPCSISLFLNGRYVFTGDALFVRGCGRTDFQGGSAESLYHSITGKLFLLPDETIVYPG
CCCCEEEEEEECCEEEEECCEEEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCEEEECC
HDYKGFVSSTIGEEKKWNPRIANRSLEEFQSIMDNLNLPEPKKIHEAVPANRACGKTL
CCCCHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MVDTDSMNGIQIKPLYDFASGTWTYLLLEIESKQAVLIDPVIENLERDLQLLESMEFQLV
CCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCEEEE
ATIETHMHADHITAAGNLREKTGCDSYAPEHSGATCATHFLKDNDEIVIGKLNMKVIHTP
EEEEHHHCCCCEEECCCCCHHCCCCCCCCCCCCCCEEEHEECCCCCEEEEEEEEEEEECC
GHTPCSISLFLNGRYVFTGDALFVRGCGRTDFQGGSAESLYHSITGKLFLLPDETIVYPG
CCCCEEEEEEECCEEEEECCEEEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCEEEECC
HDYKGFVSSTIGEEKKWNPRIANRSLEEFQSIMDNLNLPEPKKIHEAVPANRACGKTL
CCCCHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]