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The map label for this gene is groEL

Identifier: 183221715

GI number: 183221715

Start: 2415565

End: 2417214

Strand: Direct

Name: groEL

Synonym: LEPBI_I2344

Alternate gene names: 183221715

Gene position: 2415565-2417214 (Clockwise)

Preceding gene: 183221714

Following gene: 183221716

Centisome position: 67.11

GC content: 44.42

Gene sequence:

>1650_bases
ATGGCTAAAACAATCGAATTTGATGAAACAGCACGTAGAAAACTTCTTAGCGGAGTGAACAAACTCGCTAACGCAGTGAA
GGTGACACTTGGACCAAAAGGTCGTAACGTAGTCATCGACAAAAAATTTGGATCTCCTACCATCACAAAAGACGGTGTTA
CAGTTGCGAAAGAAATCGAACTAGAAGATGCAATTGAAAACATGGGCGCTCAAATGGTGAAAGAAGTTTCTACCAAAACG
AACGACATCGCCGGAGACGGAACAACCACTGCAACCATCCTTGCACAAGCCATCATCAACGAAGGTTTGAAAAACGTAAC
TGCGGGTGCAAACCCAATGGCACTCAAACACGGAATTGACAAAGCAGTTGTTGTCGCTGTGGAAGAAATCAAAAAACACG
CAATCAAAATCAATAGCAAAGCAGAATACGCAAACGTTGCAACGATCTCTGCAAACAATGATCCTGAAATCGGTAACCTC
ATTGCACAAGCTTTTGACAAAGTAGGTAAAGAAGGTGTGATCACTGTGGATGAAGCAAAATCCATCGAGACTACTCTTGA
TATCGTAGAGGGTATGCAATTTGATCGTGGATACGTATCACCTTATATGGTAACTGATCCAGAAGCAATGATCGCAACTT
TCAACGATCCATTCATCTTAATTTACGACAAAAAAATTGCTTCGATGAAAGACCTTCTCCCAGTGCTTGAAAAAATTGCA
CAGGCGGGTAGACCACTTGTCATCATCGCAGAAGAAGTGGAAGGCGAAGCTCTTGCAACAATCGTTGTGAACACACTTCG
TAAAACCATCCAATGTGTGGCTGTAAAAGCTCCAGGGTTTGGTGATAGAAGAAAAGCAATGCTTGAAGACATCGCAATCC
TCACTGGTGGACAAGTGATTTCTGAAGACCTCGGAATGAAACTCGAAAACGCTGATGTGAAGATGCTCGGTCGCGCGAAA
AAAGTGGTCGTAGACAAAGAAAACACAACCATCATCGAAGGTGCTGGTGCTTCTAAAGACATCCAAGGCCGAGTGAACCA
AATCAAAAAACAAATCGAAGATACAACTTCTGATTACGATCGTGAAAAACTCCAAGAACGCCTTGCAAAACTTGCTGGTG
GTGTGGCTGTGATCCACGTTGGTGCTGCAACGGAAGTAGAAATGAAAGAGAAAAAAGCTCGTGTCGAAGATGCTCTTTCT
GCAACTCGTGCGGCAGTGGAAGAAGGAATTGTACCTGGTGGTGGACTCACACTACTTCGTGCACAAGATGCAGTGAAAGC
TCTTAAACTAGTTGGTGACGAACAAACTGGTGCGAACATCATCTTACGTGCATTAGAAGAACCTATCCGTATGATCACTT
CTAATGCTGGTCTTGAAGGATCTGTGATTGTTGAACAAGCTCGTGCCCGTAAAGGAAACGAAGGCTTCAACGCACTCACT
ATGGTTTGGGAAGACTTAATCAAAGCTGGTGTGGTTGACCCTGCGAAAGTGGTTCGTTCTGCCCTTCAAAATGCAGCTTC
TATTGGAGCGATGATCCTCACCACTGAAGTGACCATTACAGACAAACCTGAGCCGAAAGATGCTTCTGGTGCTGGAATGG
GCGGCATGGGAGGAATGGGTGGTATGGGAGGAATGGGAGGCATGATGTAA

Upstream 100 bases:

>100_bases
GGAACAGAAATCAAACAAGGCGGAAAAGATTTACTCATTATCCGTGAAAGCGACATCCTCGGTGTCGTAACAAACTAATC
ATAAAAGGGAATCATTAACC

Downstream 100 bases:

>100_bases
ATCATCCCCCTCATTTTTTCTTTGGTCTCTTTGGTGATCCAAACAGGCCAAACACCGAAAAGGTCTCGTGAGTCACATCC
GAGGCCTTTTTTTATGGGTG

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MAKTIEFDETARRKLLSGVNKLANAVKVTLGPKGRNVVIDKKFGSPTITKDGVTVAKEIELEDAIENMGAQMVKEVSTKT
NDIAGDGTTTATILAQAIINEGLKNVTAGANPMALKHGIDKAVVVAVEEIKKHAIKINSKAEYANVATISANNDPEIGNL
IAQAFDKVGKEGVITVDEAKSIETTLDIVEGMQFDRGYVSPYMVTDPEAMIATFNDPFILIYDKKIASMKDLLPVLEKIA
QAGRPLVIIAEEVEGEALATIVVNTLRKTIQCVAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGMKLENADVKMLGRAK
KVVVDKENTTIIEGAGASKDIQGRVNQIKKQIEDTTSDYDREKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALS
ATRAAVEEGIVPGGGLTLLRAQDAVKALKLVGDEQTGANIILRALEEPIRMITSNAGLEGSVIVEQARARKGNEGFNALT
MVWEDLIKAGVVDPAKVVRSALQNAASIGAMILTTEVTITDKPEPKDASGAGMGGMGGMGGMGGMGGMM

Sequences:

>Translated_549_residues
MAKTIEFDETARRKLLSGVNKLANAVKVTLGPKGRNVVIDKKFGSPTITKDGVTVAKEIELEDAIENMGAQMVKEVSTKT
NDIAGDGTTTATILAQAIINEGLKNVTAGANPMALKHGIDKAVVVAVEEIKKHAIKINSKAEYANVATISANNDPEIGNL
IAQAFDKVGKEGVITVDEAKSIETTLDIVEGMQFDRGYVSPYMVTDPEAMIATFNDPFILIYDKKIASMKDLLPVLEKIA
QAGRPLVIIAEEVEGEALATIVVNTLRKTIQCVAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGMKLENADVKMLGRAK
KVVVDKENTTIIEGAGASKDIQGRVNQIKKQIEDTTSDYDREKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALS
ATRAAVEEGIVPGGGLTLLRAQDAVKALKLVGDEQTGANIILRALEEPIRMITSNAGLEGSVIVEQARARKGNEGFNALT
MVWEDLIKAGVVDPAKVVRSALQNAASIGAMILTTEVTITDKPEPKDASGAGMGGMGGMGGMGGMGGMM
>Mature_548_residues
AKTIEFDETARRKLLSGVNKLANAVKVTLGPKGRNVVIDKKFGSPTITKDGVTVAKEIELEDAIENMGAQMVKEVSTKTN
DIAGDGTTTATILAQAIINEGLKNVTAGANPMALKHGIDKAVVVAVEEIKKHAIKINSKAEYANVATISANNDPEIGNLI
AQAFDKVGKEGVITVDEAKSIETTLDIVEGMQFDRGYVSPYMVTDPEAMIATFNDPFILIYDKKIASMKDLLPVLEKIAQ
AGRPLVIIAEEVEGEALATIVVNTLRKTIQCVAVKAPGFGDRRKAMLEDIAILTGGQVISEDLGMKLENADVKMLGRAKK
VVVDKENTTIIEGAGASKDIQGRVNQIKKQIEDTTSDYDREKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALSA
TRAAVEEGIVPGGGLTLLRAQDAVKALKLVGDEQTGANIILRALEEPIRMITSNAGLEGSVIVEQARARKGNEGFNALTM
VWEDLIKAGVVDPAKVVRSALQNAASIGAMILTTEVTITDKPEPKDASGAGMGGMGGMGGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=48.3992467043315, Blast_Score=481, Evalue=1e-136,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=48.3992467043315, Blast_Score=481, Evalue=1e-136,
Organism=Escherichia coli, GI1790586, Length=526, Percent_Identity=62.5475285171103, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=527, Percent_Identity=47.6280834914611, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI193210679, Length=208, Percent_Identity=48.0769230769231, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=50.1908396946565, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322350, Length=581, Percent_Identity=23.9242685025818, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322524, Length=154, Percent_Identity=26.6233766233766, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24641193, Length=526, Percent_Identity=48.0988593155894, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24641191, Length=526, Percent_Identity=48.0988593155894, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=45.6106870229008, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=45.6106870229008, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=45.6106870229008, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864606, Length=552, Percent_Identity=40.9420289855072, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=35.5805243445693, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=35.5805243445693, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_LEPBA (B0SCC0)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963345.1
- ProteinModelPortal:   B0SCC0
- SMR:   B0SCC0
- GeneID:   6389491
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2274
- HOGENOM:   HBG625289
- OMA:   EDYVDMV
- ProtClustDB:   PRK00013
- BioCyc:   LBIF355278:LBF_2274-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 58110; Mature: 57979

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIEFDETARRKLLSGVNKLANAVKVTLGPKGRNVVIDKKFGSPTITKDGVTVAKEIE
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHH
LEDAIENMGAQMVKEVSTKTNDIAGDGTTTATILAQAIINEGLKNVTAGANPMALKHGID
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
KAVVVAVEEIKKHAIKINSKAEYANVATISANNDPEIGNLIAQAFDKVGKEGVITVDEAK
HHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCH
SIETTLDIVEGMQFDRGYVSPYMVTDPEAMIATFNDPFILIYDKKIASMKDLLPVLEKIA
HHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHH
QAGRPLVIIAEEVEGEALATIVVNTLRKTIQCVAVKAPGFGDRRKAMLEDIAILTGGQVI
HCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHH
SEDLGMKLENADVKMLGRAKKVVVDKENTTIIEGAGASKDIQGRVNQIKKQIEDTTSDYD
HHHHCCEECCCCHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHH
REKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALSATRAAVEEGIVPGGGLTLLR
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
AQDAVKALKLVGDEQTGANIILRALEEPIRMITSNAGLEGSVIVEQARARKGNEGFNALT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHH
MVWEDLIKAGVVDPAKVVRSALQNAASIGAMILTTEVTITDKPEPKDASGAGMGGMGGMG
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
GMGGMGGMM
CCCCCCCCC
>Mature Secondary Structure 
AKTIEFDETARRKLLSGVNKLANAVKVTLGPKGRNVVIDKKFGSPTITKDGVTVAKEIE
CCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHH
LEDAIENMGAQMVKEVSTKTNDIAGDGTTTATILAQAIINEGLKNVTAGANPMALKHGID
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
KAVVVAVEEIKKHAIKINSKAEYANVATISANNDPEIGNLIAQAFDKVGKEGVITVDEAK
HHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCH
SIETTLDIVEGMQFDRGYVSPYMVTDPEAMIATFNDPFILIYDKKIASMKDLLPVLEKIA
HHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHH
QAGRPLVIIAEEVEGEALATIVVNTLRKTIQCVAVKAPGFGDRRKAMLEDIAILTGGQVI
HCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCHHH
SEDLGMKLENADVKMLGRAKKVVVDKENTTIIEGAGASKDIQGRVNQIKKQIEDTTSDYD
HHHHCCEECCCCHHHHCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHH
REKLQERLAKLAGGVAVIHVGAATEVEMKEKKARVEDALSATRAAVEEGIVPGGGLTLLR
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
AQDAVKALKLVGDEQTGANIILRALEEPIRMITSNAGLEGSVIVEQARARKGNEGFNALT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHH
MVWEDLIKAGVVDPAKVVRSALQNAASIGAMILTTEVTITDKPEPKDASGAGMGGMGGMG
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
GMGGMGGMM
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA