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The map label for this gene is vraR [H]

Identifier: 183221711

GI number: 183221711

Start: 2410314

End: 2410934

Strand: Direct

Name: vraR [H]

Synonym: LEPBI_I2339

Alternate gene names: 183221711

Gene position: 2410314-2410934 (Clockwise)

Preceding gene: 183221705

Following gene: 183221713

Centisome position: 66.96

GC content: 46.05

Gene sequence:

>621_bases
ATGACACCGAAAAAGAAGGTATTACTCGTTGAAGACCACGCAGTGACCAGGGTCGGCGTGAAACATGTGGTCAATGCTTC
GAATGATTTTGAAGTGGTGGGAGAAGCCGAACATTCCTCTCAAATTGCAAACCTCCTCCAAGAAACGAGACCCGATTTTG
TCCTGCTCGACCTTCGCATTCCTGGGGAAAACGTCCTCAATATGGTGAAAGATTGGAAAAAAGACCTGCCACAACTCAAA
GTGGTCACCCTCACCATGCTCGATGAACAACCCATTGTCCATTCGGCCATTGAAGCGGGAGTGGATGGGTATCTTCTGAA
GAGTGATGACTTGAGTAGCCTCACTCGGAACCTGAATGAGATTGCCTCTGGGAAAACGGTGTATTCCAAAAACCTAAAAC
TCAGCTTCAATCGAAAACCACAGGATGGTAAGGTTGCCAACAAAAAGGAAAAACAAATCCTCACTCTCCTCGGACAAGGC
AAAACCTACCAAGAAATTGGAACCGACATTGGGCTTTCCAAACGAACGGTAGAATACCATGTCGGACGTTTGAAAGACAG
ATTCAATGCGAAAACCGTTGCGGAACTCATTGGTCGCGCCAAAGAACAAATGTTAATCTAA

Upstream 100 bases:

>100_bases
AAATGAAACGGAATAGTACTAATATTGCCAGTTCTCCTCGTCTACTAGGGGGAAACGCTAGGAAGAAATCAGCTTCTTAG
ATCCAGATAAAGGGAAATTT

Downstream 100 bases:

>100_bases
ACCTCAAAGGTCGAGCAGTCTCTTCACAAGTTCGACCATCTTTTGATCGGGGGATGTTTTTGTTAAAAAAGCATCTCCCC
CAAGATCGATCACTTCTGTT

Product: two-component regulatory LuxR family response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTPKKKVLLVEDHAVTRVGVKHVVNASNDFEVVGEAEHSSQIANLLQETRPDFVLLDLRIPGENVLNMVKDWKKDLPQLK
VVTLTMLDEQPIVHSAIEAGVDGYLLKSDDLSSLTRNLNEIASGKTVYSKNLKLSFNRKPQDGKVANKKEKQILTLLGQG
KTYQEIGTDIGLSKRTVEYHVGRLKDRFNAKTVAELIGRAKEQMLI

Sequences:

>Translated_206_residues
MTPKKKVLLVEDHAVTRVGVKHVVNASNDFEVVGEAEHSSQIANLLQETRPDFVLLDLRIPGENVLNMVKDWKKDLPQLK
VVTLTMLDEQPIVHSAIEAGVDGYLLKSDDLSSLTRNLNEIASGKTVYSKNLKLSFNRKPQDGKVANKKEKQILTLLGQG
KTYQEIGTDIGLSKRTVEYHVGRLKDRFNAKTVAELIGRAKEQMLI
>Mature_205_residues
TPKKKVLLVEDHAVTRVGVKHVVNASNDFEVVGEAEHSSQIANLLQETRPDFVLLDLRIPGENVLNMVKDWKKDLPQLKV
VTLTMLDEQPIVHSAIEAGVDGYLLKSDDLSSLTRNLNEIASGKTVYSKNLKLSFNRKPQDGKVANKKEKQILTLLGQGK
TYQEIGTDIGLSKRTVEYHVGRLKDRFNAKTVAELIGRAKEQMLI

Specific function: Member of the two-component regulatory system vraS/vraR involved in the control of the cell wall peptidoglycan biosynthesis. VraR is overexpressed in strain Mu3, which leads to vancomycin resistance [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=193, Percent_Identity=27.979274611399, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787473, Length=183, Percent_Identity=31.6939890710383, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788222, Length=190, Percent_Identity=24.2105263157895, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1790102, Length=193, Percent_Identity=24.3523316062176, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786747, Length=214, Percent_Identity=26.1682242990654, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788546, Length=177, Percent_Identity=23.728813559322, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23027; Mature: 22896

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKKKVLLVEDHAVTRVGVKHVVNASNDFEVVGEAEHSSQIANLLQETRPDFVLLDLRI
CCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEEC
PGENVLNMVKDWKKDLPQLKVVTLTMLDEQPIVHSAIEAGVDGYLLKSDDLSSLTRNLNE
CCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHH
IASGKTVYSKNLKLSFNRKPQDGKVANKKEKQILTLLGQGKTYQEIGTDIGLSKRTVEYH
HHCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHH
VGRLKDRFNAKTVAELIGRAKEQMLI
HHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPKKKVLLVEDHAVTRVGVKHVVNASNDFEVVGEAEHSSQIANLLQETRPDFVLLDLRI
CCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEEC
PGENVLNMVKDWKKDLPQLKVVTLTMLDEQPIVHSAIEAGVDGYLLKSDDLSSLTRNLNE
CCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHH
IASGKTVYSKNLKLSFNRKPQDGKVANKKEKQILTLLGQGKTYQEIGTDIGLSKRTVEYH
HHCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHH
VGRLKDRFNAKTVAELIGRAKEQMLI
HHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]