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The map label for this gene is leuB [H]

Identifier: 183221692

GI number: 183221692

Start: 2387477

End: 2388556

Strand: Direct

Name: leuB [H]

Synonym: LEPBI_I2317

Alternate gene names: 183221692

Gene position: 2387477-2388556 (Clockwise)

Preceding gene: 183221691

Following gene: 183221693

Centisome position: 66.32

GC content: 43.24

Gene sequence:

>1080_bases
ATGAAAAAAGTTGCAGTTCTTGCCGGTGATGGTATCGGTCCGGAAGTTATGGAAGTGGCCCTACAGGTAGTAGGGAAAGC
ATTAGGAAACAAACGTAGCGAATTTACCTTTGAACACGCGCTAGTTGGTGGGGCAGCCATTGATGCCACTGGATTTCCAC
TCCCTGAGGAAACTTTAAAACTTTGTGAATCGGCAAGTGCTATCTTTTTTGGATCTGTTGGTGGACCTAAATGGGAAACC
CTTCCTCCGGATAGGCAACCAGAACGAGGAGCTTTGCTTCCCCTTCGCAAACACTTTGATTTATTTGCCAACCTCCGCCC
CGCGATCATTTATCCAGAATTAAAAAAAGCAAGTCCTATCCGAGGAGACATCATCGGAGATGGATTGGATATTTTGATCC
TGAGAGAACTTACGTCTGGAATCTATTTTGGAAAACCAAAAGGTAGAGAAGGAAGTGGGCCCGAAGAATTTGCTTATGAC
ACCATGCGTTATTCAAGAAGAGAAATCGAACGTATTGCAAGAACTGCATTTGATGCCGCTAGGAAACGAAATAAAAAAGT
AACGAGTATTGATAAAGCAAACGTTTTAACCACTTCTGTATTATGGAGAGAGGTAGTGGTGGAACTTCATAAAAAAGAAT
ACTCAGATTGTGTATTGGAACATCTGTATGTGGACAATGCAGCCATGCAGCTCATCGTCAAACCGAAACAATTTGATGTC
ATGTTATGTGAAAACATGTTTGGAGACATTCTTTCCGATGAGGCTTCCATCATCACCGGTTCCATTGGAATGTTACCATC
CGCGTCTCTTTCGGAATCTGGATTCGGTTTGTATGAACCTTCTGGTGGTTCGGCTCCCGATATCGCTGGGAAAGGAATTG
CAAACCCCATTGCCCAAATTCTTTCAGGAGCTCTGATGTTACGATATTCCTTTGGAATGGAAGCTGAGGCAGTTTCGATC
GAAAATGCCATTCGTACCGTTTTAAAGAAAGGATTTCGCACTGGGGATATTGCCGAAGAAGGCACAACTGTTCTTGGAAC
CAAAGAAATTGGTGTTGAAATCGAAAAGGCACTTGGATAA

Upstream 100 bases:

>100_bases
CAGCTGGAAAAGTCATTCGTATCATACCTCCACTCATCTTGAGCATTGAAAAAGCAACAGAAGGGTTGGATATTTTAGAA
TCAGTTTTAAAGGAAATGAA

Downstream 100 bases:

>100_bases
GGTACGTAGAACATGCAAGCAGGCATAGGACCCACAGGCAGACCTTATCAAATTCTCATTGCTGAGAATTCCAAATTCCA
GTCCAAACAACTCCAACAGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKKVAVLAGDGIGPEVMEVALQVVGKALGNKRSEFTFEHALVGGAAIDATGFPLPEETLKLCESASAIFFGSVGGPKWET
LPPDRQPERGALLPLRKHFDLFANLRPAIIYPELKKASPIRGDIIGDGLDILILRELTSGIYFGKPKGREGSGPEEFAYD
TMRYSRREIERIARTAFDAARKRNKKVTSIDKANVLTTSVLWREVVVELHKKEYSDCVLEHLYVDNAAMQLIVKPKQFDV
MLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGIANPIAQILSGALMLRYSFGMEAEAVSI
ENAIRTVLKKGFRTGDIAEEGTTVLGTKEIGVEIEKALG

Sequences:

>Translated_359_residues
MKKVAVLAGDGIGPEVMEVALQVVGKALGNKRSEFTFEHALVGGAAIDATGFPLPEETLKLCESASAIFFGSVGGPKWET
LPPDRQPERGALLPLRKHFDLFANLRPAIIYPELKKASPIRGDIIGDGLDILILRELTSGIYFGKPKGREGSGPEEFAYD
TMRYSRREIERIARTAFDAARKRNKKVTSIDKANVLTTSVLWREVVVELHKKEYSDCVLEHLYVDNAAMQLIVKPKQFDV
MLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGIANPIAQILSGALMLRYSFGMEAEAVSI
ENAIRTVLKKGFRTGDIAEEGTTVLGTKEIGVEIEKALG
>Mature_359_residues
MKKVAVLAGDGIGPEVMEVALQVVGKALGNKRSEFTFEHALVGGAAIDATGFPLPEETLKLCESASAIFFGSVGGPKWET
LPPDRQPERGALLPLRKHFDLFANLRPAIIYPELKKASPIRGDIIGDGLDILILRELTSGIYFGKPKGREGSGPEEFAYD
TMRYSRREIERIARTAFDAARKRNKKVTSIDKANVLTTSVLWREVVVELHKKEYSDCVLEHLYVDNAAMQLIVKPKQFDV
MLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGIANPIAQILSGALMLRYSFGMEAEAVSI
ENAIRTVLKKGFRTGDIAEEGTTVLGTKEIGVEIEKALG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=340, Percent_Identity=30.5882352941176, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI28178821, Length=334, Percent_Identity=29.6407185628743, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178816, Length=334, Percent_Identity=29.6407185628743, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4758582, Length=357, Percent_Identity=28.8515406162465, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI28178838, Length=328, Percent_Identity=29.2682926829268, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI28178819, Length=166, Percent_Identity=34.3373493975904, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI87081683, Length=349, Percent_Identity=63.8968481375358, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1788101, Length=350, Percent_Identity=34.8571428571429, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1787381, Length=357, Percent_Identity=24.0896358543417, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=357, Percent_Identity=27.4509803921569, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=340, Percent_Identity=31.4705882352941, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=341, Percent_Identity=31.0850439882698, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=364, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=43.9024390243902, Blast_Score=275, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=34.9431818181818, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=352, Percent_Identity=31.25, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=348, Percent_Identity=30.1724137931034, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24643268, Length=361, Percent_Identity=31.3019390581717, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24643270, Length=361, Percent_Identity=31.3019390581717, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=27.6967930029155, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078635, Length=338, Percent_Identity=29.585798816568, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161078637, Length=338, Percent_Identity=29.585798816568, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161078633, Length=338, Percent_Identity=29.585798816568, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24650122, Length=338, Percent_Identity=29.585798816568, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078639, Length=336, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281362242, Length=351, Percent_Identity=29.3447293447293, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648872, Length=351, Percent_Identity=29.3447293447293, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=27.6162790697674, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38834; Mature: 38834

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVAVLAGDGIGPEVMEVALQVVGKALGNKRSEFTFEHALVGGAAIDATGFPLPEETLK
CCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEECCCCCCCCHHHHH
LCESASAIFFGSVGGPKWETLPPDRQPERGALLPLRKHFDLFANLRPAIIYPELKKASPI
HHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCHHCCCCC
RGDIIGDGLDILILRELTSGIYFGKPKGREGSGPEEFAYDTMRYSRREIERIARTAFDAA
CCCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRNKKVTSIDKANVLTTSVLWREVVVELHKKEYSDCVLEHLYVDNAAMQLIVKPKQFDV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCHHHH
MLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGIANPIAQI
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHH
LSGALMLRYSFGMEAEAVSIENAIRTVLKKGFRTGDIAEEGTTVLGTKEIGVEIEKALG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHCCHHHHHCC
>Mature Secondary Structure
MKKVAVLAGDGIGPEVMEVALQVVGKALGNKRSEFTFEHALVGGAAIDATGFPLPEETLK
CCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEECCCCCCCCHHHHH
LCESASAIFFGSVGGPKWETLPPDRQPERGALLPLRKHFDLFANLRPAIIYPELKKASPI
HHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCHHCCCCC
RGDIIGDGLDILILRELTSGIYFGKPKGREGSGPEEFAYDTMRYSRREIERIARTAFDAA
CCCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRNKKVTSIDKANVLTTSVLWREVVVELHKKEYSDCVLEHLYVDNAAMQLIVKPKQFDV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCHHHH
MLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGIANPIAQI
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHH
LSGALMLRYSFGMEAEAVSIENAIRTVLKKGFRTGDIAEEGTTVLGTKEIGVEIEKALG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA