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The map label for this gene is dnaB [H]

Identifier: 183221478

GI number: 183221478

Start: 2191697

End: 2193040

Strand: Reverse

Name: dnaB [H]

Synonym: LEPBI_I2096

Alternate gene names: 183221478

Gene position: 2193040-2191697 (Counterclockwise)

Preceding gene: 183221479

Following gene: 183221477

Centisome position: 60.92

GC content: 43.9

Gene sequence:

>1344_bases
ATGAATTCTAACCCCCTTCAGGAATTAGAGTCTGAGAAGAACTTAATCGGTTACTTACTCATGCGAGGGGTAGCCGGGCA
GGAAGACTTAGGTCTTAGCCCGGAAGACTTTTATATGGATACCCATAGAAGAGTCTTCGAAGCGGTCACAGACCTCATAA
ATGAAGGGATCAATATCGACCTAGTAACGGTCACAAACCAAATGCGAGAAAAACGTCTCTTTAAAGACGAATCTCGCGAT
CTGGAATACATCACTTCCCTATATAAAGATACAGTCCCCTTTCAGCCGTTAGAGTATTATGTGCGACGTGTCAAACGTGT
TTCCGATAGACGGAAATATGTTGAGGCCCTCTCACAAGCGATCGATAAAGTCAAAATTGAACCGGGTGAAAACGATTCTG
TATTTAGTTTGATCGAACAGTCACTGATGGATATCTCTCGCCAAGAGAGATCCAAAGGTTTACGGAAAGTCAAAGACGAT
GCCAATGCCCTCATTGATTACATTAAAAATGTAGTCGCGGCAAGTCAAAATGGAACCGGTGGGATCAATGGATTAAAAAC
CCATTTTACGGGACTTGATATGGCTACCACAGGTCTTAAGTCACACGAACTCATGATCCTTGCTGCACGTCCAGGGAATG
GTAAAACTACATTTGCCTTAAACATTGCCGCCAATGCCGCCTTAAAAGAACGTAAAACAGTTGTTATTTTCTCACTCGAG
ATGAGCCGGATCGAATTACTTCTCAAACTCATCAGTGCGGATGCAAGGATTGATTCTTATGCGTTAAAGGCGGGAACTTT
AACCTCGGCTCAAATGACACAACTGAAAGACAGTATTGGAAACATCACTTCTGCAAGTTTGTACATTGATGACTCAGGGT
ATTTGACGATCCAAGAGTTTTCGGCAAGACTCCGACAACTTCGTACCACTGAAGAAGTGGGTCTTGTGATTGTGGATTAT
TTACAACTTATGAGTGACCCAAAAGCCGCTATGGGTGGAAGGCAACAAGAGGTTGCCAATATTTCCAGGGGCTTAAAACA
AATGGCAAGGGAAGTTGGTTGTCCCATCATCGCTTTATCGCAGATGAATCGTTCCATTGAAAACCGCTCCAAAGACCAAA
GGCCACAACTTTCCGACTTACGGGAGTCAGGTGCCATTGAGCAGGATGCGGACATTGTATGTTTTATTTATCGGGAAGAA
ATGGTGAAACCTCCCGAAGAGTTAGACCCCAATAAAAGGGGGATGGCTGAGATCATCATCGCCAAAAACAGAGCGGGAGC
AACGGCCGATTTTCCATTGATGTTCAATCCGAAGATCAGCCGTTTCGATAACGTTCCATTATAA

Upstream 100 bases:

>100_bases
TGGTGAGTTTAAAATCAAAGTTCGTTTGGCAGAAGGTGTTGTTCCTGGGATCATTGTAAAAGTCGTCGGCCAAGCATAGT
CTAACACCAACACTTTTTCG

Downstream 100 bases:

>100_bases
AGAGGTTTTCAATTGAATCAGTGGGTCACATCAGAATACAAAAATAGAATTAGTGCAACGAGTGTATCGGATGCATCTGT
CGGTAAAACTTTATTCCTTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNSNPLQELESEKNLIGYLLMRGVAGQEDLGLSPEDFYMDTHRRVFEAVTDLINEGINIDLVTVTNQMREKRLFKDESRD
LEYITSLYKDTVPFQPLEYYVRRVKRVSDRRKYVEALSQAIDKVKIEPGENDSVFSLIEQSLMDISRQERSKGLRKVKDD
ANALIDYIKNVVAASQNGTGGINGLKTHFTGLDMATTGLKSHELMILAARPGNGKTTFALNIAANAALKERKTVVIFSLE
MSRIELLLKLISADARIDSYALKAGTLTSAQMTQLKDSIGNITSASLYIDDSGYLTIQEFSARLRQLRTTEEVGLVIVDY
LQLMSDPKAAMGGRQQEVANISRGLKQMAREVGCPIIALSQMNRSIENRSKDQRPQLSDLRESGAIEQDADIVCFIYREE
MVKPPEELDPNKRGMAEIIIAKNRAGATADFPLMFNPKISRFDNVPL

Sequences:

>Translated_447_residues
MNSNPLQELESEKNLIGYLLMRGVAGQEDLGLSPEDFYMDTHRRVFEAVTDLINEGINIDLVTVTNQMREKRLFKDESRD
LEYITSLYKDTVPFQPLEYYVRRVKRVSDRRKYVEALSQAIDKVKIEPGENDSVFSLIEQSLMDISRQERSKGLRKVKDD
ANALIDYIKNVVAASQNGTGGINGLKTHFTGLDMATTGLKSHELMILAARPGNGKTTFALNIAANAALKERKTVVIFSLE
MSRIELLLKLISADARIDSYALKAGTLTSAQMTQLKDSIGNITSASLYIDDSGYLTIQEFSARLRQLRTTEEVGLVIVDY
LQLMSDPKAAMGGRQQEVANISRGLKQMAREVGCPIIALSQMNRSIENRSKDQRPQLSDLRESGAIEQDADIVCFIYREE
MVKPPEELDPNKRGMAEIIIAKNRAGATADFPLMFNPKISRFDNVPL
>Mature_447_residues
MNSNPLQELESEKNLIGYLLMRGVAGQEDLGLSPEDFYMDTHRRVFEAVTDLINEGINIDLVTVTNQMREKRLFKDESRD
LEYITSLYKDTVPFQPLEYYVRRVKRVSDRRKYVEALSQAIDKVKIEPGENDSVFSLIEQSLMDISRQERSKGLRKVKDD
ANALIDYIKNVVAASQNGTGGINGLKTHFTGLDMATTGLKSHELMILAARPGNGKTTFALNIAANAALKERKTVVIFSLE
MSRIELLLKLISADARIDSYALKAGTLTSAQMTQLKDSIGNITSASLYIDDSGYLTIQEFSARLRQLRTTEEVGLVIVDY
LQLMSDPKAAMGGRQQEVANISRGLKQMAREVGCPIIALSQMNRSIENRSKDQRPQLSDLRESGAIEQDADIVCFIYREE
MVKPPEELDPNKRGMAEIIIAKNRAGATADFPLMFNPKISRFDNVPL

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=448, Percent_Identity=34.5982142857143, Blast_Score=241, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50098; Mature: 50098

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNPLQELESEKNLIGYLLMRGVAGQEDLGLSPEDFYMDTHRRVFEAVTDLINEGINID
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
LVTVTNQMREKRLFKDESRDLEYITSLYKDTVPFQPLEYYVRRVKRVSDRRKYVEALSQA
EEECHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IDKVKIEPGENDSVFSLIEQSLMDISRQERSKGLRKVKDDANALIDYIKNVVAASQNGTG
HHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GINGLKTHFTGLDMATTGLKSHELMILAARPGNGKTTFALNIAANAALKERKTVVIFSLE
CCCCHHHHHCCCHHHHCCCCCCEEEEEEECCCCCCEEEEEEEHHHHHHHCCCEEEEEEEC
MSRIELLLKLISADARIDSYALKAGTLTSAQMTQLKDSIGNITSASLYIDDSGYLTIQEF
HHHHHHHHHHHHCCCCHHHHEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHH
SARLRQLRTTEEVGLVIVDYLQLMSDPKAAMGGRQQEVANISRGLKQMAREVGCPIIALS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEHH
QMNRSIENRSKDQRPQLSDLRESGAIEQDADIVCFIYREEMVKPPEELDPNKRGMAEIII
HHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEHHHCCCCHHCCCCCCCCEEEEE
AKNRAGATADFPLMFNPKISRFDNVPL
EECCCCCCCCCCEEECCCHHCCCCCCC
>Mature Secondary Structure
MNSNPLQELESEKNLIGYLLMRGVAGQEDLGLSPEDFYMDTHRRVFEAVTDLINEGINID
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
LVTVTNQMREKRLFKDESRDLEYITSLYKDTVPFQPLEYYVRRVKRVSDRRKYVEALSQA
EEECHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IDKVKIEPGENDSVFSLIEQSLMDISRQERSKGLRKVKDDANALIDYIKNVVAASQNGTG
HHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GINGLKTHFTGLDMATTGLKSHELMILAARPGNGKTTFALNIAANAALKERKTVVIFSLE
CCCCHHHHHCCCHHHHCCCCCCEEEEEEECCCCCCEEEEEEEHHHHHHHCCCEEEEEEEC
MSRIELLLKLISADARIDSYALKAGTLTSAQMTQLKDSIGNITSASLYIDDSGYLTIQEF
HHHHHHHHHHHHCCCCHHHHEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEEHHHH
SARLRQLRTTEEVGLVIVDYLQLMSDPKAAMGGRQQEVANISRGLKQMAREVGCPIIALS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEHH
QMNRSIENRSKDQRPQLSDLRESGAIEQDADIVCFIYREEMVKPPEELDPNKRGMAEIII
HHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEHHHCCCCHHCCCCCCCCEEEEE
AKNRAGATADFPLMFNPKISRFDNVPL
EECCCCCCCCCCEEECCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]