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The map label for this gene is aspS

Identifier: 183221477

GI number: 183221477

Start: 2189879

End: 2191684

Strand: Reverse

Name: aspS

Synonym: LEPBI_I2095

Alternate gene names: 183221477

Gene position: 2191684-2189879 (Counterclockwise)

Preceding gene: 183221478

Following gene: 183221476

Centisome position: 60.89

GC content: 43.02

Gene sequence:

>1806_bases
TTGAATCAGTGGGTCACATCAGAATACAAAAATAGAATTAGTGCAACGAGTGTATCGGATGCATCTGTCGGTAAAACTTT
ATTCCTTTCTGGATGGGCTTTCCGTTACCGCGACCAGGGGGGGGTGATCTTCATTGACCTGCGTGATCGATCTGGAATCT
TACAAATTGTTGCAAGAAAAGAAATCTTAGGCGATGACTTCTCTAAAGTCGAAAAAATTCGTTCTGAATTCGTAATTGCG
GTAAAGGGAAAACTTTCCCTAAGGGATGCTGATTCTATCAATCCAAAGATGGAAACCGGCAAATACGAGTTAATCGCCGA
ATCTGTCGAAATTTTAAACTCATCCAAAACTCCTCCTTTTACATTAGATGAGTTTGATCCATCGGGTGAAGAAATCCGTT
TGAAGTATCGTTACCTTGATATGAGACGTGAAGAACTTCGGGATCGTTTAGTGTTGCGACACAAACTAACTTTTGCTTTG
CGAGAGTATTTGGACAGTAAGTCATTTTTGGAAATTGAAACCCCCATTTTAAATAAGTCCACTCCAGAAGGAGCTAGGGA
CTTTCTTGTGCCTTCTCGTTTGAATGCAGGTGAGTTTTATGCACTCCCACAGTCCCCGCAACTTTTTAAGCAGATCCTAA
TGATTGGTGGAATGGAACGATACTTCCAAATTGTAAAATGTTTTCGGGATGAAGACTTACGAGCGGACCGCCAACCTGAA
TTCACACAACTCGATATGGAGTTTTCCTTTGTCACAGAAGAAGACATCCGCCGTGAGATTGAAGCGATGTGGGCGTTTGC
CTTAAAGAAAGTGTTCCAGTTAGAAGTGAACGCACCTTTTATGACCATGCCGTATCATGTGGCGATGGAAGAATACGGTT
CGGACAAACCAGACATTCGTTTTGGAATGAAACTTGTGAATGTATCAGAACATGTGAAATCTTGTGACTTCCAAGTGTTT
ACGGGTGCCATCACGAGTGGTGGAGTTGTCAAAGCGATTTGTGTTCCAGGTGGATCTGTGATTTCTAGAAAAGAAATTGA
AGACCTCACGGCATGGCTTTCCCGCGACTACCGTGCCAAAGGCCTGGCTTATATGAAACATGGGGCAAATGGGCTGGAAT
CCACCATCACCAAACGGTTTTCCCCGGAAGCACTTGAGGCTATTGCCAAAGCCGTTGGATCCAAAGAAGGGGATATGGTA
TTTTTTGGTGCTGATTCTTCCAAAATTGTGAATGCTTCTCTTGGTGCCTTACGTTTGAAATTATCCGAAAAATACGATCC
ACCTAAGGTCCCTTATAGTTTCCATTGGGTCGTAGACTTTCCTATGTTTGAAATTGATGAAACAACCAAATCATGGACCT
TCCTTCATCATCCATTCACATCTCCGAAGGAAGAAGACTTTCAGAAACTAAGAGATTGGAAGGACGGGAAAGAAGTTGAC
CTTTCTTCGATTGGGGCGAAAGCTTATGATTTAGTTTTGAATGGAACAGAGATTGGTGGTGGATCCATTCGTATCCACAA
CCCAGAAATCCAAAGTCTCGTACTCGAAGCGATTGGAATTGGGGAAGAAGATGCCAAATCCAAGTTTGGATTTTTACTCG
ATGCACTCTCGTTTGGAGCGCCACCTCATGGTGGGATTGCCTTTGGTGTGGATCGGATCATGATGTTACTCACTGGTGGG
ACTTCCATCCGTGATGTGATTGCTTTCCCAAAAACACAAAAAGGAACTTGTATGATGAGTGAAGCGCCAGGTCCTGTGGA
AGCAAAACAATTAGAAGAACTCAAACTTAGGGTAGTCACAATCTAA

Upstream 100 bases:

>100_bases
CATCGCCAAAAACAGAGCGGGAGCAACGGCCGATTTTCCATTGATGTTCAATCCGAAGATCAGCCGTTTCGATAACGTTC
CATTATAAAGAGGTTTTCAA

Downstream 100 bases:

>100_bases
CATGGATGAGAGTTTTACATTTCAGGAAGAATCCCTCTACCAGACGGTATGTGGGATCAGTGACAGCAAACTCCCGTTAT
GGGAAAGTCGACTGAGTGTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MNQWVTSEYKNRISATSVSDASVGKTLFLSGWAFRYRDQGGVIFIDLRDRSGILQIVARKEILGDDFSKVEKIRSEFVIA
VKGKLSLRDADSINPKMETGKYELIAESVEILNSSKTPPFTLDEFDPSGEEIRLKYRYLDMRREELRDRLVLRHKLTFAL
REYLDSKSFLEIETPILNKSTPEGARDFLVPSRLNAGEFYALPQSPQLFKQILMIGGMERYFQIVKCFRDEDLRADRQPE
FTQLDMEFSFVTEEDIRREIEAMWAFALKKVFQLEVNAPFMTMPYHVAMEEYGSDKPDIRFGMKLVNVSEHVKSCDFQVF
TGAITSGGVVKAICVPGGSVISRKEIEDLTAWLSRDYRAKGLAYMKHGANGLESTITKRFSPEALEAIAKAVGSKEGDMV
FFGADSSKIVNASLGALRLKLSEKYDPPKVPYSFHWVVDFPMFEIDETTKSWTFLHHPFTSPKEEDFQKLRDWKDGKEVD
LSSIGAKAYDLVLNGTEIGGGSIRIHNPEIQSLVLEAIGIGEEDAKSKFGFLLDALSFGAPPHGGIAFGVDRIMMLLTGG
TSIRDVIAFPKTQKGTCMMSEAPGPVEAKQLEELKLRVVTI

Sequences:

>Translated_601_residues
MNQWVTSEYKNRISATSVSDASVGKTLFLSGWAFRYRDQGGVIFIDLRDRSGILQIVARKEILGDDFSKVEKIRSEFVIA
VKGKLSLRDADSINPKMETGKYELIAESVEILNSSKTPPFTLDEFDPSGEEIRLKYRYLDMRREELRDRLVLRHKLTFAL
REYLDSKSFLEIETPILNKSTPEGARDFLVPSRLNAGEFYALPQSPQLFKQILMIGGMERYFQIVKCFRDEDLRADRQPE
FTQLDMEFSFVTEEDIRREIEAMWAFALKKVFQLEVNAPFMTMPYHVAMEEYGSDKPDIRFGMKLVNVSEHVKSCDFQVF
TGAITSGGVVKAICVPGGSVISRKEIEDLTAWLSRDYRAKGLAYMKHGANGLESTITKRFSPEALEAIAKAVGSKEGDMV
FFGADSSKIVNASLGALRLKLSEKYDPPKVPYSFHWVVDFPMFEIDETTKSWTFLHHPFTSPKEEDFQKLRDWKDGKEVD
LSSIGAKAYDLVLNGTEIGGGSIRIHNPEIQSLVLEAIGIGEEDAKSKFGFLLDALSFGAPPHGGIAFGVDRIMMLLTGG
TSIRDVIAFPKTQKGTCMMSEAPGPVEAKQLEELKLRVVTI
>Mature_601_residues
MNQWVTSEYKNRISATSVSDASVGKTLFLSGWAFRYRDQGGVIFIDLRDRSGILQIVARKEILGDDFSKVEKIRSEFVIA
VKGKLSLRDADSINPKMETGKYELIAESVEILNSSKTPPFTLDEFDPSGEEIRLKYRYLDMRREELRDRLVLRHKLTFAL
REYLDSKSFLEIETPILNKSTPEGARDFLVPSRLNAGEFYALPQSPQLFKQILMIGGMERYFQIVKCFRDEDLRADRQPE
FTQLDMEFSFVTEEDIRREIEAMWAFALKKVFQLEVNAPFMTMPYHVAMEEYGSDKPDIRFGMKLVNVSEHVKSCDFQVF
TGAITSGGVVKAICVPGGSVISRKEIEDLTAWLSRDYRAKGLAYMKHGANGLESTITKRFSPEALEAIAKAVGSKEGDMV
FFGADSSKIVNASLGALRLKLSEKYDPPKVPYSFHWVVDFPMFEIDETTKSWTFLHHPFTSPKEEDFQKLRDWKDGKEVD
LSSIGAKAYDLVLNGTEIGGGSIRIHNPEIQSLVLEAIGIGEEDAKSKFGFLLDALSFGAPPHGGIAFGVDRIMMLLTGG
TSIRDVIAFPKTQKGTCMMSEAPGPVEAKQLEELKLRVVTI

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=38.7312186978297, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI5031815, Length=273, Percent_Identity=27.4725274725275, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI194272210, Length=273, Percent_Identity=27.4725274725275, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=47.6190476190476, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1790571, Length=253, Percent_Identity=26.4822134387352, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1789256, Length=242, Percent_Identity=26.0330578512397, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=604, Percent_Identity=35.7615894039735, Blast_Score=308, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI71994340, Length=262, Percent_Identity=24.8091603053435, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=280, Percent_Identity=25, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=280, Percent_Identity=25, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=635, Percent_Identity=32.4409448818898, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6320242, Length=151, Percent_Identity=27.8145695364238, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321807, Length=319, Percent_Identity=25.0783699059561, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=147, Percent_Identity=29.9319727891156, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323011, Length=249, Percent_Identity=26.9076305220884, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24584738, Length=607, Percent_Identity=37.0675453047776, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640851, Length=252, Percent_Identity=25.7936507936508, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24640849, Length=254, Percent_Identity=26.3779527559055, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_LEPBA (B0SB35)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963119.1
- ProteinModelPortal:   B0SB35
- SMR:   B0SB35
- GeneID:   6388336
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2041
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   LBIF355278:LBF_2041-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67694; Mature: 67694

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQWVTSEYKNRISATSVSDASVGKTLFLSGWAFRYRDQGGVIFIDLRDRSGILQIVARK
CCCCCCHHHHCCCCCCCCCCCCCCCEEEEECEEEEEECCCCEEEEEECCCCCHHHHHHHH
EILGDDFSKVEKIRSEFVIAVKGKLSLRDADSINPKMETGKYELIAESVEILNSSKTPPF
HHHCCCHHHHHHHHHCEEEEEECEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
TLDEFDPSGEEIRLKYRYLDMRREELRDRLVLRHKLTFALREYLDSKSFLEIETPILNKS
ECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
TPEGARDFLVPSRLNAGEFYALPQSPQLFKQILMIGGMERYFQIVKCFRDEDLRADRQPE
CCCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCC
FTQLDMEFSFVTEEDIRREIEAMWAFALKKVFQLEVNAPFMTMPYHVAMEEYGSDKPDIR
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCHHHHHHHHCCCCCCHH
FGMKLVNVSEHVKSCDFQVFTGAITSGGVVKAICVPGGSVISRKEIEDLTAWLSRDYRAK
HHHEEECHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHC
GLAYMKHGANGLESTITKRFSPEALEAIAKAVGSKEGDMVFFGADSSKIVNASLGALRLK
CHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCEEEEE
LSEKYDPPKVPYSFHWVVDFPMFEIDETTKSWTFLHHPFTSPKEEDFQKLRDWKDGKEVD
ECCCCCCCCCCEEEEEEEECCEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCC
LSSIGAKAYDLVLNGTEIGGGSIRIHNPEIQSLVLEAIGIGEEDAKSKFGFLLDALSFGA
HHHCCCEEEEEEEECCEECCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
PPHGGIAFGVDRIMMLLTGGTSIRDVIAFPKTQKGTCMMSEAPGPVEAKQLEELKLRVVT
CCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEECCCCCCCCHHHHHCEEEEEEE
I
C
>Mature Secondary Structure
MNQWVTSEYKNRISATSVSDASVGKTLFLSGWAFRYRDQGGVIFIDLRDRSGILQIVARK
CCCCCCHHHHCCCCCCCCCCCCCCCEEEEECEEEEEECCCCEEEEEECCCCCHHHHHHHH
EILGDDFSKVEKIRSEFVIAVKGKLSLRDADSINPKMETGKYELIAESVEILNSSKTPPF
HHHCCCHHHHHHHHHCEEEEEECEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
TLDEFDPSGEEIRLKYRYLDMRREELRDRLVLRHKLTFALREYLDSKSFLEIETPILNKS
ECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
TPEGARDFLVPSRLNAGEFYALPQSPQLFKQILMIGGMERYFQIVKCFRDEDLRADRQPE
CCCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCC
FTQLDMEFSFVTEEDIRREIEAMWAFALKKVFQLEVNAPFMTMPYHVAMEEYGSDKPDIR
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEECCCCEEECCHHHHHHHHCCCCCCHH
FGMKLVNVSEHVKSCDFQVFTGAITSGGVVKAICVPGGSVISRKEIEDLTAWLSRDYRAK
HHHEEECHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHC
GLAYMKHGANGLESTITKRFSPEALEAIAKAVGSKEGDMVFFGADSSKIVNASLGALRLK
CHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCEEEEE
LSEKYDPPKVPYSFHWVVDFPMFEIDETTKSWTFLHHPFTSPKEEDFQKLRDWKDGKEVD
ECCCCCCCCCCEEEEEEEECCEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCC
LSSIGAKAYDLVLNGTEIGGGSIRIHNPEIQSLVLEAIGIGEEDAKSKFGFLLDALSFGA
HHHCCCEEEEEEEECCEECCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
PPHGGIAFGVDRIMMLLTGGTSIRDVIAFPKTQKGTCMMSEAPGPVEAKQLEELKLRVVT
CCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEECCCCCCCCHHHHHCEEEEEEE
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA