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The map label for this gene is sppA [H]

Identifier: 183221224

GI number: 183221224

Start: 1928834

End: 1930468

Strand: Direct

Name: sppA [H]

Synonym: LEPBI_I1838

Alternate gene names: 183221224

Gene position: 1928834-1930468 (Clockwise)

Preceding gene: 183221221

Following gene: 183221227

Centisome position: 53.58

GC content: 39.45

Gene sequence:

>1635_bases
GTGTTTCGAATTCTTTTTTTACTTCTTTTTTTGCCATTCCGAGTTTTGTACCTATTTTACCTACGTCTAAGCCTTCTTTT
CCAACGGAACCGAGAGGTCTATGAATTGGAAATGCCCCCCCAGTTTGAGGACTCCTATAAATCATTTTTTGTGAAAAAAT
TCCAAGGAAAGGAAGAAACCATCACTCGATTGGAACTCCTTGTACTACTGCGAACCTTACAAAAAAATCCGAAAATCAAA
ACCCTCGATATCAGTTTACCTCCATTGGAATGGACTCTTTCGGAATTTTATGAAATCAGAAACGAACTCAAAGCCATCCG
AGACAGTGGAAAAACCATCCGTATGTTTGCCAAAGAAGGTGGCATGGGGACATTACTCCTCCTAACAATCGCAAATGAAG
CTTACCTGGCACCAGAATCCGAGTTTACAGTTTTATTACCAAGCGCCGAACCAATGTTCTTTGGTAAGTTTTTAAAAACC
TGGGGGATCGAAGTCCAAGCTTTTGCTTCCGGTCCCTACAAGTCCTTTGCCGAAAGTTTCACTCGGGGAGAGTTCTCAAA
AGAAGCACGAAAAAACTTAGAATCACTCATCTTAAACCTTCGCACTCTCCTATTAGAATCACTCACCAATGGAAAAAAAT
CGCTAGAACCATTCTTTTACAAACCGATGTTATCTGCGGATGACTTACTTACGGAAGGAGTGATCCATGGCATCAAAACC
GAATCAGAGTTTTTTTCGGAAGATCGAAAGATTTTTTCGAGAGTGTATCCGATTGTCTACCAAACAGTAAAAGAATTTTC
CTTATTTCCCAAAGCAAACAAGGAAGTGGTCATTCTTCCTTTAGAAGGAGGGATCACCGGTGGAGATTATTTACATAAAA
ATAGAGAAAATGGAAAAATAGAAGCATTTTCCTTAATCCCCACCTTAAAAGCATTAGGTGAAGATAAAAAAATCAAAGCT
GTGATCTTAGAAATTTCCTCTCCCGGTGGGTCTGCTTTTTATTCGGAACAAATCCACCAAGAAATTTTGGAATTAAAGAA
AACCAAACCGGTCACAGCCTATTTTAAAGACACAGTGGCAAGCGGTGGGTATTACATTGCAACTGCAGCCGATTACATCA
CAGCCTCTCCTGTTTGTATCACAGGGTCCATTGGAGCGGTGAGTATCCGTGCCAATTTACAGAAATTGTATAAAAAATTT
CATTTGAATAAAGAAGCAGTTGGATTTTACCCTTTCCGGGACATCCATTCTGAGTTCCAACCTCTCTCCAAACAAAGTGT
ATTGTACTTAGAATCACAAATCAAAAAAATAGAAGGTTTGTTTTACAAACGTGTTTCCGAAGGAAGGGACATCCCCCTAA
AAGAACTTCCGAACATTGGAATGGGAAGAGTTTATTTACCAACAACCGAAAACAAAATTGTTGATTCACTTGGTGGTTTA
CTCGATGCCATTGAATTCGTGAAGGAGAAGTTAGGTGGTAAAGGGATATACCTCACAGAAGAATTGCCTTCCTATAACTT
AAAAAATAAAATCCCTCTCCTTGGAGGTCTACTCAGTGAATTGAAAATTCTGGAGTCACTTGGCGAAGTATCCTTACTTT
CCCCGATCAAACTCCACTGGAAAAATCGTATCTGA

Upstream 100 bases:

>100_bases
CGAGCGCCACGAATGCGTATAAAGGAATCCACATCGGATAGCTTGTTTTCCTCTTTCATTCTGGAAAGAATTTTAAGGAA
TGGAAGGAAGAGGACTTATC

Downstream 100 bases:

>100_bases
GTGGAAACAGATTCGAATTGAGGTGATTCTAAACGTATCCAAACGGAAACTGGCAGACAAAAGTGATTGGTTTTGAAGGG
ATGGAAGTTAGATCGGACAG

Product: S49 family peptidase

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MFRILFLLLFLPFRVLYLFYLRLSLLFQRNREVYELEMPPQFEDSYKSFFVKKFQGKEETITRLELLVLLRTLQKNPKIK
TLDISLPPLEWTLSEFYEIRNELKAIRDSGKTIRMFAKEGGMGTLLLLTIANEAYLAPESEFTVLLPSAEPMFFGKFLKT
WGIEVQAFASGPYKSFAESFTRGEFSKEARKNLESLILNLRTLLLESLTNGKKSLEPFFYKPMLSADDLLTEGVIHGIKT
ESEFFSEDRKIFSRVYPIVYQTVKEFSLFPKANKEVVILPLEGGITGGDYLHKNRENGKIEAFSLIPTLKALGEDKKIKA
VILEISSPGGSAFYSEQIHQEILELKKTKPVTAYFKDTVASGGYYIATAADYITASPVCITGSIGAVSIRANLQKLYKKF
HLNKEAVGFYPFRDIHSEFQPLSKQSVLYLESQIKKIEGLFYKRVSEGRDIPLKELPNIGMGRVYLPTTENKIVDSLGGL
LDAIEFVKEKLGGKGIYLTEELPSYNLKNKIPLLGGLLSELKILESLGEVSLLSPIKLHWKNRI

Sequences:

>Translated_544_residues
MFRILFLLLFLPFRVLYLFYLRLSLLFQRNREVYELEMPPQFEDSYKSFFVKKFQGKEETITRLELLVLLRTLQKNPKIK
TLDISLPPLEWTLSEFYEIRNELKAIRDSGKTIRMFAKEGGMGTLLLLTIANEAYLAPESEFTVLLPSAEPMFFGKFLKT
WGIEVQAFASGPYKSFAESFTRGEFSKEARKNLESLILNLRTLLLESLTNGKKSLEPFFYKPMLSADDLLTEGVIHGIKT
ESEFFSEDRKIFSRVYPIVYQTVKEFSLFPKANKEVVILPLEGGITGGDYLHKNRENGKIEAFSLIPTLKALGEDKKIKA
VILEISSPGGSAFYSEQIHQEILELKKTKPVTAYFKDTVASGGYYIATAADYITASPVCITGSIGAVSIRANLQKLYKKF
HLNKEAVGFYPFRDIHSEFQPLSKQSVLYLESQIKKIEGLFYKRVSEGRDIPLKELPNIGMGRVYLPTTENKIVDSLGGL
LDAIEFVKEKLGGKGIYLTEELPSYNLKNKIPLLGGLLSELKILESLGEVSLLSPIKLHWKNRI
>Mature_544_residues
MFRILFLLLFLPFRVLYLFYLRLSLLFQRNREVYELEMPPQFEDSYKSFFVKKFQGKEETITRLELLVLLRTLQKNPKIK
TLDISLPPLEWTLSEFYEIRNELKAIRDSGKTIRMFAKEGGMGTLLLLTIANEAYLAPESEFTVLLPSAEPMFFGKFLKT
WGIEVQAFASGPYKSFAESFTRGEFSKEARKNLESLILNLRTLLLESLTNGKKSLEPFFYKPMLSADDLLTEGVIHGIKT
ESEFFSEDRKIFSRVYPIVYQTVKEFSLFPKANKEVVILPLEGGITGGDYLHKNRENGKIEAFSLIPTLKALGEDKKIKA
VILEISSPGGSAFYSEQIHQEILELKKTKPVTAYFKDTVASGGYYIATAADYITASPVCITGSIGAVSIRANLQKLYKKF
HLNKEAVGFYPFRDIHSEFQPLSKQSVLYLESQIKKIEGLFYKRVSEGRDIPLKELPNIGMGRVYLPTTENKIVDSLGGL
LDAIEFVKEKLGGKGIYLTEELPSYNLKNKIPLLGGLLSELKILESLGEVSLLSPIKLHWKNRI

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=412, Percent_Identity=24.7572815533981, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 61872; Mature: 61872

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRILFLLLFLPFRVLYLFYLRLSLLFQRNREVYELEMPPQFEDSYKSFFVKKFQGKEET
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHH
ITRLELLVLLRTLQKNPKIKTLDISLPPLEWTLSEFYEIRNELKAIRDSGKTIRMFAKEG
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
GMGTLLLLTIANEAYLAPESEFTVLLPSAEPMFFGKFLKTWGIEVQAFASGPYKSFAESF
CCCEEEEEEECCCCEECCCCCEEEEECCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
TRGEFSKEARKNLESLILNLRTLLLESLTNGKKSLEPFFYKPMLSADDLLTEGVIHGIKT
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHCCCCCHHHHHHHHHHHCCCC
ESEFFSEDRKIFSRVYPIVYQTVKEFSLFPKANKEVVILPLEGGITGGDYLHKNRENGKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCE
EAFSLIPTLKALGEDKKIKAVILEISSPGGSAFYSEQIHQEILELKKTKPVTAYFKDTVA
EEEEHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
SGGYYIATAADYITASPVCITGSIGAVSIRANLQKLYKKFHLNKEAVGFYPFRDIHSEFQ
CCCEEEEEEHHHHCCCCEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
PLSKQSVLYLESQIKKIEGLFYKRVSEGRDIPLKELPNIGMGRVYLPTTENKIVDSLGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCCHHHHHHHHHH
LDAIEFVKEKLGGKGIYLTEELPSYNLKNKIPLLGGLLSELKILESLGEVSLLSPIKLHW
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCHHHHC
KNRI
CCCC
>Mature Secondary Structure
MFRILFLLLFLPFRVLYLFYLRLSLLFQRNREVYELEMPPQFEDSYKSFFVKKFQGKEET
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHH
ITRLELLVLLRTLQKNPKIKTLDISLPPLEWTLSEFYEIRNELKAIRDSGKTIRMFAKEG
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
GMGTLLLLTIANEAYLAPESEFTVLLPSAEPMFFGKFLKTWGIEVQAFASGPYKSFAESF
CCCEEEEEEECCCCEECCCCCEEEEECCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
TRGEFSKEARKNLESLILNLRTLLLESLTNGKKSLEPFFYKPMLSADDLLTEGVIHGIKT
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHCCCCCHHHHHHHHHHHCCCC
ESEFFSEDRKIFSRVYPIVYQTVKEFSLFPKANKEVVILPLEGGITGGDYLHKNRENGKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCCE
EAFSLIPTLKALGEDKKIKAVILEISSPGGSAFYSEQIHQEILELKKTKPVTAYFKDTVA
EEEEHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
SGGYYIATAADYITASPVCITGSIGAVSIRANLQKLYKKFHLNKEAVGFYPFRDIHSEFQ
CCCEEEEEEHHHHCCCCEEEECCCCEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
PLSKQSVLYLESQIKKIEGLFYKRVSEGRDIPLKELPNIGMGRVYLPTTENKIVDSLGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCCHHHHHHHHHH
LDAIEFVKEKLGGKGIYLTEELPSYNLKNKIPLLGGLLSELKILESLGEVSLLSPIKLHW
HHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCCHHHHC
KNRI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]