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The map label for this gene is pmbA [C]

Identifier: 183221095

GI number: 183221095

Start: 1782446

End: 1783828

Strand: Direct

Name: pmbA [C]

Synonym: LEPBI_I1709

Alternate gene names: 183221095

Gene position: 1782446-1783828 (Clockwise)

Preceding gene: 183221094

Following gene: 183221096

Centisome position: 49.52

GC content: 39.55

Gene sequence:

>1383_bases
ATGATGGATCGTGGTGCGATAGAAAAACGATTGAATCACCAAAAAGATTTACTTTCGAATTTAGTAAAGAAAGCCAAGTC
AAATGGAATGGATCAAGTAGAGATTTATTCCAGTTATGGATATTCCGAAGATGTCAGTTTAGAAAAAAATGATTTAAACA
ACTGTACAGCGAATGAAGAAAATATGTTTGGAATTCGAGTGATCCACGAAGGGAACCAAGGATTTATTATTTCTAATCAT
ATTCCGAGTTTGTACGAGTCCATAGAAGAAGCTTATCAATTGGCCAAAAGCCAAACTACTCCTGATCTTGATTTGATTTT
ACCTGAAGCCCGACCAATCGACAAACAATTTGATACCTATGATATGACTCTTGATTCAATGGGAATCGAAGATTTGGTTT
CTTATGCCAAAGAAGCACTTGGTTGGCGAAATGAGTTTTATGGAAAGGTCAATATTGATTCAGGTGAGTTTTCGCTTAGC
AAGGGTTACAAATTAATCGTATCCTCAAAAGGTGTAATGGCACATGAACTTGGTGCGGAACTTTCTGCCTCTGTGATGGG
TATGGGTGTGGACGGGGAGCTTGTCGGTAGTTTTGATTACGATTCAGCGAGTGGGTTTCAAAAAGATCAGTTCCAATCAC
TTTGGAAAAAAGCCTTTTTTAATTTTGGCGATAAATGTATGGGAGCTCTTTATTCGAAACCAATTTCAGGGTTCCAAGGA
AAAGTATTATTACCTCCTGAAGCTGTGTATTCATTTTTTCTCGGACTTTTTATTGGATCTTTGAACGGGACAAGTTTACG
AAAAGGAAAATCGAAGATGGAAGGGAAATTAGGCGAAAAAGTAGCCTCTTCTCTACTCTCCATTTGGGATGATCCAACAA
ATATTGGTTTTATGGGATCGACTGGTTTTGATCGCGAAGGAATGCCTACCAGTTTTAAAAAAGTTCTCACCGAAGGGGTT
TTGGAATCCTACTTTTATAATACCTATGAAGCTAAAAAAGCAGGGCTTATCACATCCAATGGATCGGCGACTGGTGGGGC
TCAAAGCCTTCCTGGTTGTGGACCGAAACAATTACAAATTGCACCAGGTACTTCCTCGAAAGATGAGTTTTTCAAACAAC
CAGGAAAAACCTTGTTTGTGAATCGAATTTCTGGAACAAAAGATGGTGCTTCTGGTGATTTCTCTGGAGTTGTGAAAGGA
GGATACCTTTTGGAAGCGGGTGAAAAAATACCGGTCCGAGAGGTGCAAATTGTGGGCAATGCCTTTGATGCCTTACACCA
AATAGAAGCCATCTCAAAAGAAGGCGAATTGCTTGGTGAATCATCCTTTGTGCCCTACCTCTTACTCGATGGATTTACCG
TCACAGGTGTTTCGGAAAATTAA

Upstream 100 bases:

>100_bases
TTAAGTGGGATTTAGGTTCGGGCCACTGCGGGAAAGGCCAACCTGCGAAAGTAGATGCCGGTGGACCATATGTTCGAACT
AAAGTGTTGTTAGGTGGTAA

Downstream 100 bases:

>100_bases
TCATGCCACAAGAAATTTTAGAAAGGCCACCAGGTGCATCCTTTTTTCTCATCGTATCCTATGAGAATGAAGATACTTTT
TTTGAGTTAAAAAATTTAGC

Product: putative protease PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MMDRGAIEKRLNHQKDLLSNLVKKAKSNGMDQVEIYSSYGYSEDVSLEKNDLNNCTANEENMFGIRVIHEGNQGFIISNH
IPSLYESIEEAYQLAKSQTTPDLDLILPEARPIDKQFDTYDMTLDSMGIEDLVSYAKEALGWRNEFYGKVNIDSGEFSLS
KGYKLIVSSKGVMAHELGAELSASVMGMGVDGELVGSFDYDSASGFQKDQFQSLWKKAFFNFGDKCMGALYSKPISGFQG
KVLLPPEAVYSFFLGLFIGSLNGTSLRKGKSKMEGKLGEKVASSLLSIWDDPTNIGFMGSTGFDREGMPTSFKKVLTEGV
LESYFYNTYEAKKAGLITSNGSATGGAQSLPGCGPKQLQIAPGTSSKDEFFKQPGKTLFVNRISGTKDGASGDFSGVVKG
GYLLEAGEKIPVREVQIVGNAFDALHQIEAISKEGELLGESSFVPYLLLDGFTVTGVSEN

Sequences:

>Translated_460_residues
MMDRGAIEKRLNHQKDLLSNLVKKAKSNGMDQVEIYSSYGYSEDVSLEKNDLNNCTANEENMFGIRVIHEGNQGFIISNH
IPSLYESIEEAYQLAKSQTTPDLDLILPEARPIDKQFDTYDMTLDSMGIEDLVSYAKEALGWRNEFYGKVNIDSGEFSLS
KGYKLIVSSKGVMAHELGAELSASVMGMGVDGELVGSFDYDSASGFQKDQFQSLWKKAFFNFGDKCMGALYSKPISGFQG
KVLLPPEAVYSFFLGLFIGSLNGTSLRKGKSKMEGKLGEKVASSLLSIWDDPTNIGFMGSTGFDREGMPTSFKKVLTEGV
LESYFYNTYEAKKAGLITSNGSATGGAQSLPGCGPKQLQIAPGTSSKDEFFKQPGKTLFVNRISGTKDGASGDFSGVVKG
GYLLEAGEKIPVREVQIVGNAFDALHQIEAISKEGELLGESSFVPYLLLDGFTVTGVSEN
>Mature_460_residues
MMDRGAIEKRLNHQKDLLSNLVKKAKSNGMDQVEIYSSYGYSEDVSLEKNDLNNCTANEENMFGIRVIHEGNQGFIISNH
IPSLYESIEEAYQLAKSQTTPDLDLILPEARPIDKQFDTYDMTLDSMGIEDLVSYAKEALGWRNEFYGKVNIDSGEFSLS
KGYKLIVSSKGVMAHELGAELSASVMGMGVDGELVGSFDYDSASGFQKDQFQSLWKKAFFNFGDKCMGALYSKPISGFQG
KVLLPPEAVYSFFLGLFIGSLNGTSLRKGKSKMEGKLGEKVASSLLSIWDDPTNIGFMGSTGFDREGMPTSFKKVLTEGV
LESYFYNTYEAKKAGLITSNGSATGGAQSLPGCGPKQLQIAPGTSSKDEFFKQPGKTLFVNRISGTKDGASGDFSGVVKG
GYLLEAGEKIPVREVQIVGNAFDALHQIEAISKEGELLGESSFVPYLLLDGFTVTGVSEN

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790682, Length=447, Percent_Identity=24.3847874720358, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789640, Length=486, Percent_Identity=24.6913580246914, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 50027; Mature: 50027

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDRGAIEKRLNHQKDLLSNLVKKAKSNGMDQVEIYSSYGYSEDVSLEKNDLNNCTANEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
NMFGIRVIHEGNQGFIISNHIPSLYESIEEAYQLAKSQTTPDLDLILPEARPIDKQFDTY
CEEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCH
DMTLDSMGIEDLVSYAKEALGWRNEFYGKVNIDSGEFSLSKGYKLIVSSKGVMAHELGAE
HCCHHHCCHHHHHHHHHHHHCCHHHCCEEEECCCCCEECCCCEEEEEECCCCHHHHHCCH
LSASVMGMGVDGELVGSFDYDSASGFQKDQFQSLWKKAFFNFGDKCMGALYSKPISGFQG
HHHHHEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
KVLLPPEAVYSFFLGLFIGSLNGTSLRKGKSKMEGKLGEKVASSLLSIWDDPTNIGFMGS
EEECCHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
TGFDREGMPTSFKKVLTEGVLESYFYNTYEAKKAGLITSNGSATGGAQSLPGCGPKQLQI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCEEECCCCCCCCCCCCCCCCCCCEEE
APGTSSKDEFFKQPGKTLFVNRISGTKDGASGDFSGVVKGGYLLEAGEKIPVREVQIVGN
CCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEECCCEEEECCCCCCCHHEEEHHH
AFDALHQIEAISKEGELLGESSFVPYLLLDGFTVTGVSEN
HHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEECCCCC
>Mature Secondary Structure
MMDRGAIEKRLNHQKDLLSNLVKKAKSNGMDQVEIYSSYGYSEDVSLEKNDLNNCTANEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
NMFGIRVIHEGNQGFIISNHIPSLYESIEEAYQLAKSQTTPDLDLILPEARPIDKQFDTY
CEEEEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCH
DMTLDSMGIEDLVSYAKEALGWRNEFYGKVNIDSGEFSLSKGYKLIVSSKGVMAHELGAE
HCCHHHCCHHHHHHHHHHHHCCHHHCCEEEECCCCCEECCCCEEEEEECCCCHHHHHCCH
LSASVMGMGVDGELVGSFDYDSASGFQKDQFQSLWKKAFFNFGDKCMGALYSKPISGFQG
HHHHHEECCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
KVLLPPEAVYSFFLGLFIGSLNGTSLRKGKSKMEGKLGEKVASSLLSIWDDPTNIGFMGS
EEECCHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECC
TGFDREGMPTSFKKVLTEGVLESYFYNTYEAKKAGLITSNGSATGGAQSLPGCGPKQLQI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCEEECCCCCCCCCCCCCCCCCCCEEE
APGTSSKDEFFKQPGKTLFVNRISGTKDGASGDFSGVVKGGYLLEAGEKIPVREVQIVGN
CCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEECCCEEEECCCCCCCHHEEEHHH
AFDALHQIEAISKEGELLGESSFVPYLLLDGFTVTGVSEN
HHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]