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The map label for this gene is lipA

Identifier: 183220849

GI number: 183220849

Start: 1524432

End: 1525352

Strand: Reverse

Name: lipA

Synonym: LEPBI_I1462

Alternate gene names: 183220849

Gene position: 1525352-1524432 (Counterclockwise)

Preceding gene: 183220853

Following gene: 183220848

Centisome position: 42.37

GC content: 45.49

Gene sequence:

>921_bases
ATGAATCCATTAAAAAAGAAACCTCGCTCCAAAAATCTAAACCCCACCGTTCCCCTCCCGGACTGGATGAAGGTGCGAGT
GAGCTTTCCTACGGATTCGGATGCACTTTCTGTTGTCCGAGCGGAGGTGGAATCCAAAGAGCTCCATACAGTTTGCGAAT
CCGCAAGTTGCCCAAACCTCAACCATTGTTGGAACCGCAAAACTGCCACATATATGCTCGCAGGAGACATTTGTACAAGA
AGGTGCCAATACTGTGATGTTGCCTTTGGAAAACCAAAACCCTTAGATTCCCTCGAACCAGAACGAGTCGCAAGATCTGT
GCAGTCGCTTGGACTGAGACATGTGGTCCTCACCGCTGTGAACCGGGATGATTTAAAAGATGGAGGGGCAAGTCACTTTG
CAGAAACCATCACCAAAATCAAAACCTACCATAAAGATTGTACGATCGAAGTTCTCATCCCTGACTTTAAGGCAAAAGAA
GATTCCTTACAGATTCTGTATGCGGCGAAACCGAATATCATCAATCATAATATCGAAACTGTGGAATCACTCTTCCCTAC
CATCACCCCACAAAAAAACTACAAACGTTCTCTCGAAGTATTAGCTCATATTGCAAACCATGGATTTCTCACGAAAAGTG
GGATCATTCTTGGACTCGGGGAAACAGACGAAGATGTAAACCAATGCCTAATGGATTTATTTGCTCATGGTGTCCGGATG
TTGACAATCGGTCAGTACCTACAGCCAGGCCCAACCCACTACCCAGTGCAAAGTTTCGTCCGCCCAGAAACCTTTGTCAT
GTGGAAAGAAACGGCATACAAGATTGGATTTAAAACGGTAGCTTCTGGACCACTCGTTCGGTCTTCCTACCATGCAGATG
AATATTTCCATGAGGAATCCCAAATTCTACCGACAGAGTAG

Upstream 100 bases:

>100_bases
CTTTCTACGGGAACCTAAACCTAATTTGGCAAGAAATGCATTGATCCTCATGATTTTCCATTTTACAAAGTGGCCTCATT
TCAAAAAGTCTAAAGAAGGT

Downstream 100 bases:

>100_bases
ATATGAACGAAGAGGAATCTGAGGAATTTCAAAGAGGGCTCGCAAAAACATTCTTTTTGTCCATCCTAAAGGATTTAGGC
GAAATTGATGAAACTCTTTC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNPLKKKPRSKNLNPTVPLPDWMKVRVSFPTDSDALSVVRAEVESKELHTVCESASCPNLNHCWNRKTATYMLAGDICTR
RCQYCDVAFGKPKPLDSLEPERVARSVQSLGLRHVVLTAVNRDDLKDGGASHFAETITKIKTYHKDCTIEVLIPDFKAKE
DSLQILYAAKPNIINHNIETVESLFPTITPQKNYKRSLEVLAHIANHGFLTKSGIILGLGETDEDVNQCLMDLFAHGVRM
LTIGQYLQPGPTHYPVQSFVRPETFVMWKETAYKIGFKTVASGPLVRSSYHADEYFHEESQILPTE

Sequences:

>Translated_306_residues
MNPLKKKPRSKNLNPTVPLPDWMKVRVSFPTDSDALSVVRAEVESKELHTVCESASCPNLNHCWNRKTATYMLAGDICTR
RCQYCDVAFGKPKPLDSLEPERVARSVQSLGLRHVVLTAVNRDDLKDGGASHFAETITKIKTYHKDCTIEVLIPDFKAKE
DSLQILYAAKPNIINHNIETVESLFPTITPQKNYKRSLEVLAHIANHGFLTKSGIILGLGETDEDVNQCLMDLFAHGVRM
LTIGQYLQPGPTHYPVQSFVRPETFVMWKETAYKIGFKTVASGPLVRSSYHADEYFHEESQILPTE
>Mature_306_residues
MNPLKKKPRSKNLNPTVPLPDWMKVRVSFPTDSDALSVVRAEVESKELHTVCESASCPNLNHCWNRKTATYMLAGDICTR
RCQYCDVAFGKPKPLDSLEPERVARSVQSLGLRHVVLTAVNRDDLKDGGASHFAETITKIKTYHKDCTIEVLIPDFKAKE
DSLQILYAAKPNIINHNIETVESLFPTITPQKNYKRSLEVLAHIANHGFLTKSGIILGLGETDEDVNQCLMDLFAHGVRM
LTIGQYLQPGPTHYPVQSFVRPETFVMWKETAYKIGFKTVASGPLVRSSYHADEYFHEESQILPTE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=39.3728222996516, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=37.9032258064516, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=47.2924187725632, Blast_Score=274, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=42.5087108013937, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=307, Percent_Identity=42.671009771987, Blast_Score=233, Evalue=3e-62,
Organism=Drosophila melanogaster, GI221513272, Length=297, Percent_Identity=42.0875420875421, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_LEPBA (B0SGV6)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962501.1
- ProteinModelPortal:   B0SGV6
- SMR:   B0SGV6
- GeneID:   6387872
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1409
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   LBIF355278:LBF_1409-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34469; Mature: 34469

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLKKKPRSKNLNPTVPLPDWMKVRVSFPTDSDALSVVRAEVESKELHTVCESASCPNL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
NHCWNRKTATYMLAGDICTRRCQYCDVAFGKPKPLDSLEPERVARSVQSLGLRHVVLTAV
HHHHCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEEEEEEC
NRDDLKDGGASHFAETITKIKTYHKDCTIEVLIPDFKAKEDSLQILYAAKPNIINHNIET
CHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCHHH
VESLFPTITPQKNYKRSLEVLAHIANHGFLTKSGIILGLGETDEDVNQCLMDLFAHGVRM
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEECCCCHHHHHHHHHHHHHCCEEE
LTIGQYLQPGPTHYPVQSFVRPETFVMWKETAYKIGFKTVASGPLVRSSYHADEYFHEES
EHHHHHCCCCCCCCCHHHHCCCCEEEEEHHHHHHHCHHHHCCCCCCCCCCCHHHHHCCCC
QILPTE
CCCCCC
>Mature Secondary Structure
MNPLKKKPRSKNLNPTVPLPDWMKVRVSFPTDSDALSVVRAEVESKELHTVCESASCPNL
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
NHCWNRKTATYMLAGDICTRRCQYCDVAFGKPKPLDSLEPERVARSVQSLGLRHVVLTAV
HHHHCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHEEEEEEC
NRDDLKDGGASHFAETITKIKTYHKDCTIEVLIPDFKAKEDSLQILYAAKPNIINHNIET
CHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCHHH
VESLFPTITPQKNYKRSLEVLAHIANHGFLTKSGIILGLGETDEDVNQCLMDLFAHGVRM
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEECCCCHHHHHHHHHHHHHCCEEE
LTIGQYLQPGPTHYPVQSFVRPETFVMWKETAYKIGFKTVASGPLVRSSYHADEYFHEES
EHHHHHCCCCCCCCCHHHHCCCCEEEEEHHHHHHHCHHHHCCCCCCCCCCCHHHHHCCCC
QILPTE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA