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The map label for this gene is waaA [H]

Identifier: 183220722

GI number: 183220722

Start: 1387015

End: 1388292

Strand: Reverse

Name: waaA [H]

Synonym: LEPBI_I1332

Alternate gene names: 183220722

Gene position: 1388292-1387015 (Counterclockwise)

Preceding gene: 183220723

Following gene: 183220721

Centisome position: 38.57

GC content: 39.98

Gene sequence:

>1278_bases
ATGGTATATTTTTTTTATAACCTACTCATCTGTACGATATGGATTTTCCTCAAACTTGTTTCCCTCTTTTCGAAACAAAT
ACGTCAAGAACTCCATAAACGGAAACAATCTTACAAACAAATATTTTTGAAATCCCCAAATGGAAAATCTGTCATCTGGT
TCCATTCTGCAAGTGTAGGAGAACTCGACCAAGCAAAGGCTCTCCAAGAAACAGTGCGAAGGCATAGACCCGATCTCTTC
ATCATCCAATCGGTATTTTCATCCTCAGTGAAAGAAGGGGCATTCTCTGATCCCTTAGCTGATCTTTATTTTTACTTACC
CTTTGATCTTCCTTTTGCCTATGAGAAACTCTTTCGGTTTTTTAAACCAAAGTTTTTATTCATCATGGCTTGGGACACCT
GGCCCAATCTGCTAAAAAATGCATCCAAGTTTGGAACCAAATCCTACCTTTGTTGTGCCAGTTTGTCCTCTGCGTCTACC
CGTAAAAATCTACTGGTACGACTTCTCACAAAATCTTCCTTCCAGTACCTTTCAGGAATTTATCCAAGCCATCCATTGAT
GGCCAAAGAATTTGAAGGGTTTGTTTCGGAGGGAACCGATTTTCTTGTACTCGGAGACACAAGGTTTGAATCGGTATGGA
ACAAATTGGAAACCAAATCCCCGAATCCAAAGTTTACTGAATTTGTTTTGCACCAAAAAAAATTTTTGGCAAAACATCGA
CCTGTGATCCTTGGATCCACCTACCCCATTTGCGAATCTTATTTTTTAAGCTATTTAGAATCAAATCAGGATGAATGTTC
CTATTGGATCTTTCCGCACAAATGGGAGAAGGAAAGGATGTTGGATATGAAATCCAAACTTGAGACTTTCGGTTCTGCCG
CCGTTTTTTCCGAGTTAAAGGAAGGTGATCCACTTCCTAAATTTTTACTCTTTGATGTTTTGGGAATCCTTGCCTTTGCT
TACCAGTATGGAAGTTTTGCTTATGTAGGTGGGGCTTTCCATCACCGTATCCACAATACCATCGAACCGGCTGCCCTAGG
CCTTGCCATCATCACGGGACCAAAGATCCAAAACGCCCCCGAAGCGATCGTTATGCAAGGATTAGGTGGTCTTTTTAAAA
CAGAAAATGAAGCTCATTTTGTCACTCGCTTCCAATCTCTCGTCAAAAACAAAGAACTACGGGAAAAGATGGGAAATACG
AATCGAAACTTTGTTGTAGAAAATAGAGGTGCATCGGAAAAGATTTATAACCGAGTTTTTCCGTATGACAAAATATAA

Upstream 100 bases:

>100_bases
GTTTCATTTGTACTTTGATAATTTATGTTTTGTGATTGATAAACGTACTTTCAGTTACCCTGGTTCAGAAGTGAATGACA
CTTGGGGTAACAAACGCTAA

Downstream 100 bases:

>100_bases
AATTGCCGCCGTTTCTTTAAATACCACTCCTCTTGATTTTTTAGGCAATTTTGAAACCATTCAATCTGCGATCCTTTCCA
AGGAAACAAGGGATGCCGAT

Product: putative 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MVYFFYNLLICTIWIFLKLVSLFSKQIRQELHKRKQSYKQIFLKSPNGKSVIWFHSASVGELDQAKALQETVRRHRPDLF
IIQSVFSSSVKEGAFSDPLADLYFYLPFDLPFAYEKLFRFFKPKFLFIMAWDTWPNLLKNASKFGTKSYLCCASLSSAST
RKNLLVRLLTKSSFQYLSGIYPSHPLMAKEFEGFVSEGTDFLVLGDTRFESVWNKLETKSPNPKFTEFVLHQKKFLAKHR
PVILGSTYPICESYFLSYLESNQDECSYWIFPHKWEKERMLDMKSKLETFGSAAVFSELKEGDPLPKFLLFDVLGILAFA
YQYGSFAYVGGAFHHRIHNTIEPAALGLAIITGPKIQNAPEAIVMQGLGGLFKTENEAHFVTRFQSLVKNKELREKMGNT
NRNFVVENRGASEKIYNRVFPYDKI

Sequences:

>Translated_425_residues
MVYFFYNLLICTIWIFLKLVSLFSKQIRQELHKRKQSYKQIFLKSPNGKSVIWFHSASVGELDQAKALQETVRRHRPDLF
IIQSVFSSSVKEGAFSDPLADLYFYLPFDLPFAYEKLFRFFKPKFLFIMAWDTWPNLLKNASKFGTKSYLCCASLSSAST
RKNLLVRLLTKSSFQYLSGIYPSHPLMAKEFEGFVSEGTDFLVLGDTRFESVWNKLETKSPNPKFTEFVLHQKKFLAKHR
PVILGSTYPICESYFLSYLESNQDECSYWIFPHKWEKERMLDMKSKLETFGSAAVFSELKEGDPLPKFLLFDVLGILAFA
YQYGSFAYVGGAFHHRIHNTIEPAALGLAIITGPKIQNAPEAIVMQGLGGLFKTENEAHFVTRFQSLVKNKELREKMGNT
NRNFVVENRGASEKIYNRVFPYDKI
>Mature_425_residues
MVYFFYNLLICTIWIFLKLVSLFSKQIRQELHKRKQSYKQIFLKSPNGKSVIWFHSASVGELDQAKALQETVRRHRPDLF
IIQSVFSSSVKEGAFSDPLADLYFYLPFDLPFAYEKLFRFFKPKFLFIMAWDTWPNLLKNASKFGTKSYLCCASLSSAST
RKNLLVRLLTKSSFQYLSGIYPSHPLMAKEFEGFVSEGTDFLVLGDTRFESVWNKLETKSPNPKFTEFVLHQKKFLAKHR
PVILGSTYPICESYFLSYLESNQDECSYWIFPHKWEKERMLDMKSKLETFGSAAVFSELKEGDPLPKFLLFDVLGILAFA
YQYGSFAYVGGAFHHRIHNTIEPAALGLAIITGPKIQNAPEAIVMQGLGGLFKTENEAHFVTRFQSLVKNKELREKMGNT
NRNFVVENRGASEKIYNRVFPYDKI

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=431, Percent_Identity=22.2737819025522, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 49132; Mature: 49132

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYFFYNLLICTIWIFLKLVSLFSKQIRQELHKRKQSYKQIFLKSPNGKSVIWFHSASVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCC
ELDQAKALQETVRRHRPDLFIIQSVFSSSVKEGAFSDPLADLYFYLPFDLPFAYEKLFRF
CHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCHHHHHHHHH
FKPKFLFIMAWDTWPNLLKNASKFGTKSYLCCASLSSASTRKNLLVRLLTKSSFQYLSGI
HCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
YPSHPLMAKEFEGFVSEGTDFLVLGDTRFESVWNKLETKSPNPKFTEFVLHQKKFLAKHR
CCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
PVILGSTYPICESYFLSYLESNQDECSYWIFPHKWEKERMLDMKSKLETFGSAAVFSELK
CEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EGDPLPKFLLFDVLGILAFAYQYGSFAYVGGAFHHRIHNTIEPAALGLAIITGPKIQNAP
CCCCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCHHHHCEEEEECCCCCCCH
EAIVMQGLGGLFKTENEAHFVTRFQSLVKNKELREKMGNTNRNFVVENRGASEKIYNRVF
HHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCC
PYDKI
CCCCC
>Mature Secondary Structure
MVYFFYNLLICTIWIFLKLVSLFSKQIRQELHKRKQSYKQIFLKSPNGKSVIWFHSASVG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCC
ELDQAKALQETVRRHRPDLFIIQSVFSSSVKEGAFSDPLADLYFYLPFDLPFAYEKLFRF
CHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCHHHHHHHHH
FKPKFLFIMAWDTWPNLLKNASKFGTKSYLCCASLSSASTRKNLLVRLLTKSSFQYLSGI
HCCCEEEEEEECHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
YPSHPLMAKEFEGFVSEGTDFLVLGDTRFESVWNKLETKSPNPKFTEFVLHQKKFLAKHR
CCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
PVILGSTYPICESYFLSYLESNQDECSYWIFPHKWEKERMLDMKSKLETFGSAAVFSELK
CEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EGDPLPKFLLFDVLGILAFAYQYGSFAYVGGAFHHRIHNTIEPAALGLAIITGPKIQNAP
CCCCCHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCHHHHCEEEEECCCCCCCH
EAIVMQGLGGLFKTENEAHFVTRFQSLVKNKELREKMGNTNRNFVVENRGASEKIYNRVF
HHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCC
PYDKI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]