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The map label for this gene is nadE2 [H]

Identifier: 183220721

GI number: 183220721

Start: 1385088

End: 1387028

Strand: Reverse

Name: nadE2 [H]

Synonym: LEPBI_I1331

Alternate gene names: 183220721

Gene position: 1387028-1385088 (Counterclockwise)

Preceding gene: 183220722

Following gene: 183220719

Centisome position: 38.53

GC content: 41.99

Gene sequence:

>1941_bases
ATGACAAAATATAAAATTGCCGCCGTTTCTTTAAATACCACTCCTCTTGATTTTTTAGGCAATTTTGAAACCATTCAATC
TGCGATCCTTTCCAAGGAAACAAGGGATGCCGATCTCATCCTTTTTCCCGAACTTTGTATTTCTGGTTATGGATGTGAGG
ATGCATTTTTCAGGCCAAGTCTCTGGGAAAAAGGAAAAGAAGTTTTGGACAAAATCAAAACCATTTCCCCAAACCAAGTT
GTGATTGTGGGTCTTCCCATCTTTATTGATTCCTTTCTCTACAATTGTATGGCTGTCCTTTTGCATGGAAAGATCCAAGC
CATTGTTCCTAAACTCAATTTGGCAAATACGGGCGTACACTATGAACGAAGATGGTTCCATTCTCCCAAAACGTTTCTCA
ACCAATCCGTAACCATTGGTGGAATGGAAATACCATTTGGTCATTTTCTTTTTTCATGGAAGGATTTACATTTTGCCATT
GAGATCTGTGAGGACAGTTGGTCCTCTTTCAAACCTTCTCAAGTTTACAATCTTGCGGGTGCCGATGTTTTACTCTCTCC
AGGTGCCTCTCATTTTGCGATGGGCAAACAAAACATCCGAAGGCAAATCTTTACGGAAACCAGTCGGAGCCAAAACAATT
TACAAGTATTCACAAACCTTTGTGGGAATGAATCAGGAAGGATCATCTTTGAAGGTGGTGCCTTCTTTGCTTCCTGTGGC
AGACTCGTAAAGGAAGGGCCAAGGTTACATTTCACTCCCTTTGCCATCACTTCCCACTCCTTCCATCTCGATGAAATCCG
ATCTGCAAAAGCTCGTCATTTCCGAGAACCACTCCCTGAGCCAAAAACAACCCTCATTCCCAAAATCAATCTTTCGCCAC
TTAATCCTGAAGAGGCGAAAACCAGTCCATTTTTGGTTTTAGACAAACGGGATGAATCCATCTCCCCAAACGCAGATCCT
ACGGAAAACTTAAGTCCCTTTGAAGAATTCACAAAAGCCGTAAGCCTTGGTCTCTTTGATTACTTGAGAAAGTCCAAAAC
CAAAGGGTATACACTTTCACTCTCTGGGGGAGCAGACAGTGCTACCTGTGCCATCCTTGTCTCCACATTCGTGACAATCG
CCAAAAAAGAAAACGGGGACGATCATCTCACCAAACTAGGTTGGAATGAAAAAAACCTTCTTGTCACACTGTACCAAAAA
ACATCCAACAATTCTCCCATCACGGAAGAAATTGCAAAAACCTTAAGTGAAGAATTGGATTGTGAATTCCATTCCATCTC
CATTGATGAAATGGTTTCGTCTTCGGTTTCCCTCATTGAATCGGTGAAGGGAACTAAACTCAATTGGAAAGAACACGACC
TTGCCTTACAAAACATCCAAGCCCGGGTTCGCTCTCCCCTCATTTGGTTACTTGCCAATCTGAATGGTCACCTTTTATTA
TCCACAGGGAATCGTAGTGAGGCCGCTGTGGGATACACCACAATGGATGGAGATTCCTCAGGTTCCATTGCGCCACTCGC
TGGTGTGAGTAAAGAATTTTTATTAGAATGGTTAGATGACATCCAAAAGGGAAACAATCGTTATATTTCACCCAAACAAT
CCATTCAAATGTTACGGAATACAAAACCAACCGCAGAACTAAAACCCCTCACCGAACACCAGGAAGATGAGAAGGATCTA
ATGCCTTATCCCATTCTCAATTCCATTGAACGGAAACTTGTCTACCTAACAATGAATGAGTCGGAAGTATTAGAAGAACT
CAAAAAAGAATACCCTTGGGAGTCCAAGGAACAACTGTTTGGTTATTTGCAAAAATTTAAAACACTATTTAGAATCTCAC
AATGGAAACGAGAGAGGCTTCCTCCCTCGTTCCATTTGGACGAATACGGACTCGATCCAAAATCGAGTTACCGTTTTCCC
ATCCTTTCCAACGAAACCTAA

Upstream 100 bases:

>100_bases
AAAAACAAAGAACTACGGGAAAAGATGGGAAATACGAATCGAAACTTTGTTGTAGAAAATAGAGGTGCATCGGAAAAGAT
TTATAACCGAGTTTTTCCGT

Downstream 100 bases:

>100_bases
GGAGGTGGTTGGAGTCCTGCCTTTGTAAAGGCATTGGCAGCTTCCTTTTCGATTTGTTCGTTTTGGAATTGGATGTTCTT
TAAGATTTCCTGGAATTTTT

Product: putative glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MTKYKIAAVSLNTTPLDFLGNFETIQSAILSKETRDADLILFPELCISGYGCEDAFFRPSLWEKGKEVLDKIKTISPNQV
VIVGLPIFIDSFLYNCMAVLLHGKIQAIVPKLNLANTGVHYERRWFHSPKTFLNQSVTIGGMEIPFGHFLFSWKDLHFAI
EICEDSWSSFKPSQVYNLAGADVLLSPGASHFAMGKQNIRRQIFTETSRSQNNLQVFTNLCGNESGRIIFEGGAFFASCG
RLVKEGPRLHFTPFAITSHSFHLDEIRSAKARHFREPLPEPKTTLIPKINLSPLNPEEAKTSPFLVLDKRDESISPNADP
TENLSPFEEFTKAVSLGLFDYLRKSKTKGYTLSLSGGADSATCAILVSTFVTIAKKENGDDHLTKLGWNEKNLLVTLYQK
TSNNSPITEEIAKTLSEELDCEFHSISIDEMVSSSVSLIESVKGTKLNWKEHDLALQNIQARVRSPLIWLLANLNGHLLL
STGNRSEAAVGYTTMDGDSSGSIAPLAGVSKEFLLEWLDDIQKGNNRYISPKQSIQMLRNTKPTAELKPLTEHQEDEKDL
MPYPILNSIERKLVYLTMNESEVLEELKKEYPWESKEQLFGYLQKFKTLFRISQWKRERLPPSFHLDEYGLDPKSSYRFP
ILSNET

Sequences:

>Translated_646_residues
MTKYKIAAVSLNTTPLDFLGNFETIQSAILSKETRDADLILFPELCISGYGCEDAFFRPSLWEKGKEVLDKIKTISPNQV
VIVGLPIFIDSFLYNCMAVLLHGKIQAIVPKLNLANTGVHYERRWFHSPKTFLNQSVTIGGMEIPFGHFLFSWKDLHFAI
EICEDSWSSFKPSQVYNLAGADVLLSPGASHFAMGKQNIRRQIFTETSRSQNNLQVFTNLCGNESGRIIFEGGAFFASCG
RLVKEGPRLHFTPFAITSHSFHLDEIRSAKARHFREPLPEPKTTLIPKINLSPLNPEEAKTSPFLVLDKRDESISPNADP
TENLSPFEEFTKAVSLGLFDYLRKSKTKGYTLSLSGGADSATCAILVSTFVTIAKKENGDDHLTKLGWNEKNLLVTLYQK
TSNNSPITEEIAKTLSEELDCEFHSISIDEMVSSSVSLIESVKGTKLNWKEHDLALQNIQARVRSPLIWLLANLNGHLLL
STGNRSEAAVGYTTMDGDSSGSIAPLAGVSKEFLLEWLDDIQKGNNRYISPKQSIQMLRNTKPTAELKPLTEHQEDEKDL
MPYPILNSIERKLVYLTMNESEVLEELKKEYPWESKEQLFGYLQKFKTLFRISQWKRERLPPSFHLDEYGLDPKSSYRFP
ILSNET
>Mature_645_residues
TKYKIAAVSLNTTPLDFLGNFETIQSAILSKETRDADLILFPELCISGYGCEDAFFRPSLWEKGKEVLDKIKTISPNQVV
IVGLPIFIDSFLYNCMAVLLHGKIQAIVPKLNLANTGVHYERRWFHSPKTFLNQSVTIGGMEIPFGHFLFSWKDLHFAIE
ICEDSWSSFKPSQVYNLAGADVLLSPGASHFAMGKQNIRRQIFTETSRSQNNLQVFTNLCGNESGRIIFEGGAFFASCGR
LVKEGPRLHFTPFAITSHSFHLDEIRSAKARHFREPLPEPKTTLIPKINLSPLNPEEAKTSPFLVLDKRDESISPNADPT
ENLSPFEEFTKAVSLGLFDYLRKSKTKGYTLSLSGGADSATCAILVSTFVTIAKKENGDDHLTKLGWNEKNLLVTLYQKT
SNNSPITEEIAKTLSEELDCEFHSISIDEMVSSSVSLIESVKGTKLNWKEHDLALQNIQARVRSPLIWLLANLNGHLLLS
TGNRSEAAVGYTTMDGDSSGSIAPLAGVSKEFLLEWLDDIQKGNNRYISPKQSIQMLRNTKPTAELKPLTEHQEDEKDLM
PYPILNSIERKLVYLTMNESEVLEELKKEYPWESKEQLFGYLQKFKTLFRISQWKRERLPPSFHLDEYGLDPKSSYRFPI
LSNET

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=687, Percent_Identity=26.0553129548763, Blast_Score=220, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17538756, Length=688, Percent_Identity=27.906976744186, Blast_Score=214, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321865, Length=687, Percent_Identity=28.0931586608443, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24641841, Length=679, Percent_Identity=27.0986745213549, Blast_Score=205, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24641843, Length=679, Percent_Identity=27.0986745213549, Blast_Score=205, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 72909; Mature: 72778

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYKIAAVSLNTTPLDFLGNFETIQSAILSKETRDADLILFPELCISGYGCEDAFFRPS
CCCEEEEEEEECCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEHHHHHCCCCCCCCCCCCH
LWEKGKEVLDKIKTISPNQVVIVGLPIFIDSFLYNCMAVLLHGKIQAIVPKLNLANTGVH
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
YERRWFHSPKTFLNQSVTIGGMEIPFGHFLFSWKDLHFAIEICEDSWSSFKPSQVYNLAG
HHHHHCCCCHHHHCCCEEECCEECCHHHHHHHHHHHEEEEEEHHCCCCCCCCCHHHCCCC
ADVLLSPGASHFAMGKQNIRRQIFTETSRSQNNLQVFTNLCGNESGRIIFEGGAFFASCG
CCEEECCCCHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHH
RLVKEGPRLHFTPFAITSHSFHLDEIRSAKARHFREPLPEPKTTLIPKINLSPLNPEEAK
HHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHCC
TSPFLVLDKRDESISPNADPTENLSPFEEFTKAVSLGLFDYLRKSKTKGYTLSLSGGADS
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
ATCAILVSTFVTIAKKENGDDHLTKLGWNEKNLLVTLYQKTSNNSPITEEIAKTLSEELD
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHC
CEFHSISIDEMVSSSVSLIESVKGTKLNWKEHDLALQNIQARVRSPLIWLLANLNGHLLL
CCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEE
STGNRSEAAVGYTTMDGDSSGSIAPLAGVSKEFLLEWLDDIQKGNNRYISPKQSIQMLRN
EECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
TKPTAELKPLTEHQEDEKDLMPYPILNSIERKLVYLTMNESEVLEELKKEYPWESKEQLF
CCCCCCCCCCHHCCCCCCCCCCCHHHHHHCCEEEEEEECHHHHHHHHHHHCCCCCHHHHH
GYLQKFKTLFRISQWKRERLPPSFHLDEYGLDPKSSYRFPILSNET
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKYKIAAVSLNTTPLDFLGNFETIQSAILSKETRDADLILFPELCISGYGCEDAFFRPS
CCEEEEEEEECCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEHHHHHCCCCCCCCCCCCH
LWEKGKEVLDKIKTISPNQVVIVGLPIFIDSFLYNCMAVLLHGKIQAIVPKLNLANTGVH
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCC
YERRWFHSPKTFLNQSVTIGGMEIPFGHFLFSWKDLHFAIEICEDSWSSFKPSQVYNLAG
HHHHHCCCCHHHHCCCEEECCEECCHHHHHHHHHHHEEEEEEHHCCCCCCCCCHHHCCCC
ADVLLSPGASHFAMGKQNIRRQIFTETSRSQNNLQVFTNLCGNESGRIIFEGGAFFASCG
CCEEECCCCHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCHHHHHHH
RLVKEGPRLHFTPFAITSHSFHLDEIRSAKARHFREPLPEPKTTLIPKINLSPLNPEEAK
HHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHCC
TSPFLVLDKRDESISPNADPTENLSPFEEFTKAVSLGLFDYLRKSKTKGYTLSLSGGADS
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
ATCAILVSTFVTIAKKENGDDHLTKLGWNEKNLLVTLYQKTSNNSPITEEIAKTLSEELD
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHC
CEFHSISIDEMVSSSVSLIESVKGTKLNWKEHDLALQNIQARVRSPLIWLLANLNGHLLL
CCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEE
STGNRSEAAVGYTTMDGDSSGSIAPLAGVSKEFLLEWLDDIQKGNNRYISPKQSIQMLRN
EECCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
TKPTAELKPLTEHQEDEKDLMPYPILNSIERKLVYLTMNESEVLEELKKEYPWESKEQLF
CCCCCCCCCCHHCCCCCCCCCCCHHHHHHCCEEEEEEECHHHHHHHHHHHCCCCCHHHHH
GYLQKFKTLFRISQWKRERLPPSFHLDEYGLDPKSSYRFPILSNET
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]