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The map label for this gene is leuA1 [H]

Identifier: 183220681

GI number: 183220681

Start: 1346973

End: 1348508

Strand: Direct

Name: leuA1 [H]

Synonym: LEPBI_I1291

Alternate gene names: 183220681

Gene position: 1346973-1348508 (Clockwise)

Preceding gene: 183220680

Following gene: 183220682

Centisome position: 37.42

GC content: 42.19

Gene sequence:

>1536_bases
ATGACAGAACCAAATTCCAAAATTCAAATTTTAGACGTAACATTACGTGATGGGGAACAAACCAATGGTGTGTCATTTTC
TTGGCAACAGAAGTTAAACATCACGAAACACCTATTAAAAGATCTAAAGACGGACCGAGTCGAAATTGCCAGTGCTCGAG
TGTCTCCTGGTGAATTTGAGGCAGTCCAAAAAATCATCGAATGGGCAAAAGTTGAGGGACTCACTGATCGAATTGAAATT
TTGGGATTTGTTGACTTTGACAAAACCGTTGAGTGGATGAAGGGAACTGGTGTCAAAGTATTAAACCTCTTAACAAAAGG
GTCACTTAACCACCTAACAAATCAACTTCGGAAAACTCCTGAAGAACATTTTAATGATATCAAACAAACTGTCGAAGTTG
CTGTAAAGGCAGGGATCACTGTGAATGTTTATTTGGAAGACTGGTCAAATGGATACTTACATTCCAGAGACTATGTCATC
CAATATCTAAAAACAGTTTCAGAATTTCCTATCAAACGTTTTTATCTCGCAGACACACTTGGTGTCATGTCACCAGAAGA
AGTGAAAAAGGCTGTCACTGATTTAGTATCACTCTTTCCAAACTTATGGTTTGAATTCCATGGCCATAACGATTATGATT
TGGCGGTGGCCAATTGTTTGGAAGCCGTGAAGGCTGGGGCGCGAGGCCTCCATGTTGCTGTCAATGGTCTTGGAGAAAGG
GCTGGCAATTCTCCATTGGAAGCAGTGGTGACTTCCCTCCATGACAAAACCAAATTTAGAACCTCCATCGTAGAAAAAGA
AATCACCAATGCCTCTAGGCTTGTCGAAGTGTTTTCAGGAAAACGTATTTCAGACAACAGGCCGATTGTGGGAGAAGATG
TGTTTACCCAAACCGCGGGTGTCCATGCCGATGGAGATAAAAAAGGAAATTTATATGCCAATCCCATCCTCCCGGAACGG
TTTGGACGCAGTCGTGTCTACGCATTAGGGAAGTTAGCTGGGAAAGCAAGCATCACTGAAAATCTAAAACAACTCGGAAT
GGTACTCTCTCCTGAAATCGAAAAGAAAGTGTTGGAACGTGTGATTGAACTCGGTGACCAAAACAAAACCGTCACAAAAG
AAGATTTGCCATACATCATCTCCGATATCTCTGGTGAAAATTTAGAATCCAGTTTCCGCATTGAGTTTTGTACTGTGACA
AGTGGGATCGGTGTGAAACCCACAGCCGATGTAAAAGTCAAATTCCAAGGAAAACTTTTGGAAGCAAAAGGGGAAGGGGA
TGGCGGTTATGATGCCTTTATGAATGCACTGGGAAAAATCTTAGAAACTCTAAAGATCCAAATTCCAAAACTCTACGATT
ATGAGGTAAGGATCCCTCCCGGTGGGAATACCAATGCCCTTGTGGAAACTGTCATCACTTGGAAAAAAGATGCGGACATC
CATCCGATTCGCACCATAGGCATTGACTCCGACCAACAAGTGGCTGCTGTAAAGGCAACCGAACGAATGTTACATATTGT
GCTTGGGAATTTATGA

Upstream 100 bases:

>100_bases
TGAAACTAGTGGAATCTGATCCTATGTTTCGACAAAATCCTGGGAGTACCCTTGTGGAAATCCTTGCTCCTTCTAAAGAA
TACATCAACCGATTGAAAGC

Downstream 100 bases:

>100_bases
TTCACTTTTTAATCGTAGGGCTTGGAAACCCTGGAGACAAATACAAACATACCCGCCATAACATCGGATTTATGATCTTG
GATCGTTTGGCTACTGAGTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTEPNSKIQILDVTLRDGEQTNGVSFSWQQKLNITKHLLKDLKTDRVEIASARVSPGEFEAVQKIIEWAKVEGLTDRIEI
LGFVDFDKTVEWMKGTGVKVLNLLTKGSLNHLTNQLRKTPEEHFNDIKQTVEVAVKAGITVNVYLEDWSNGYLHSRDYVI
QYLKTVSEFPIKRFYLADTLGVMSPEEVKKAVTDLVSLFPNLWFEFHGHNDYDLAVANCLEAVKAGARGLHVAVNGLGER
AGNSPLEAVVTSLHDKTKFRTSIVEKEITNASRLVEVFSGKRISDNRPIVGEDVFTQTAGVHADGDKKGNLYANPILPER
FGRSRVYALGKLAGKASITENLKQLGMVLSPEIEKKVLERVIELGDQNKTVTKEDLPYIISDISGENLESSFRIEFCTVT
SGIGVKPTADVKVKFQGKLLEAKGEGDGGYDAFMNALGKILETLKIQIPKLYDYEVRIPPGGNTNALVETVITWKKDADI
HPIRTIGIDSDQQVAAVKATERMLHIVLGNL

Sequences:

>Translated_511_residues
MTEPNSKIQILDVTLRDGEQTNGVSFSWQQKLNITKHLLKDLKTDRVEIASARVSPGEFEAVQKIIEWAKVEGLTDRIEI
LGFVDFDKTVEWMKGTGVKVLNLLTKGSLNHLTNQLRKTPEEHFNDIKQTVEVAVKAGITVNVYLEDWSNGYLHSRDYVI
QYLKTVSEFPIKRFYLADTLGVMSPEEVKKAVTDLVSLFPNLWFEFHGHNDYDLAVANCLEAVKAGARGLHVAVNGLGER
AGNSPLEAVVTSLHDKTKFRTSIVEKEITNASRLVEVFSGKRISDNRPIVGEDVFTQTAGVHADGDKKGNLYANPILPER
FGRSRVYALGKLAGKASITENLKQLGMVLSPEIEKKVLERVIELGDQNKTVTKEDLPYIISDISGENLESSFRIEFCTVT
SGIGVKPTADVKVKFQGKLLEAKGEGDGGYDAFMNALGKILETLKIQIPKLYDYEVRIPPGGNTNALVETVITWKKDADI
HPIRTIGIDSDQQVAAVKATERMLHIVLGNL
>Mature_510_residues
TEPNSKIQILDVTLRDGEQTNGVSFSWQQKLNITKHLLKDLKTDRVEIASARVSPGEFEAVQKIIEWAKVEGLTDRIEIL
GFVDFDKTVEWMKGTGVKVLNLLTKGSLNHLTNQLRKTPEEHFNDIKQTVEVAVKAGITVNVYLEDWSNGYLHSRDYVIQ
YLKTVSEFPIKRFYLADTLGVMSPEEVKKAVTDLVSLFPNLWFEFHGHNDYDLAVANCLEAVKAGARGLHVAVNGLGERA
GNSPLEAVVTSLHDKTKFRTSIVEKEITNASRLVEVFSGKRISDNRPIVGEDVFTQTAGVHADGDKKGNLYANPILPERF
GRSRVYALGKLAGKASITENLKQLGMVLSPEIEKKVLERVIELGDQNKTVTKEDLPYIISDISGENLESSFRIEFCTVTS
GIGVKPTADVKVKFQGKLLEAKGEGDGGYDAFMNALGKILETLKIQIPKLYDYEVRIPPGGNTNALVETVITWKKDADIH
PIRTIGIDSDQQVAAVKATERMLHIVLGNL

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=448, Percent_Identity=28.3482142857143, Blast_Score=146, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=400, Percent_Identity=28.25, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320071, Length=397, Percent_Identity=28.2115869017632, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 56715; Mature: 56583

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPNSKIQILDVTLRDGEQTNGVSFSWQQKLNITKHLLKDLKTDRVEIASARVSPGEFE
CCCCCCCEEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEHHHCCCCCHHHH
AVQKIIEWAKVEGLTDRIEILGFVDFDKTVEWMKGTGVKVLNLLTKGSLNHLTNQLRKTP
HHHHHHHHHHHCCCCCHHHEEEEECHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCH
EEHFNDIKQTVEVAVKAGITVNVYLEDWSNGYLHSRDYVIQYLKTVSEFPIKRFYLADTL
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEECHHHHHHHHHHHHHCCHHHHHHHHHH
GVMSPEEVKKAVTDLVSLFPNLWFEFHGHNDYDLAVANCLEAVKAGARGLHVAVNGLGER
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHC
AGNSPLEAVVTSLHDKTKFRTSIVEKEITNASRLVEVFSGKRISDNRPIVGEDVFTQTAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHCCC
VHADGDKKGNLYANPILPERFGRSRVYALGKLAGKASITENLKQLGMVLSPEIEKKVLER
CCCCCCCCCCEEECCCCHHHHCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHH
VIELGDQNKTVTKEDLPYIISDISGENLESSFRIEFCTVTSGIGVKPTADVKVKFQGKLL
HHHCCCCCCCCCHHCCCHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECEEE
EAKGEGDGGYDAFMNALGKILETLKIQIPKLYDYEVRIPPGGNTNALVETVITWKKDADI
EECCCCCCCHHHHHHHHHHHHHHHHHCCCCEECEEEEECCCCCCHHHHHHHHHHCCCCCC
HPIRTIGIDSDQQVAAVKATERMLHIVLGNL
CCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEPNSKIQILDVTLRDGEQTNGVSFSWQQKLNITKHLLKDLKTDRVEIASARVSPGEFE
CCCCCCEEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEHHHCCCCCHHHH
AVQKIIEWAKVEGLTDRIEILGFVDFDKTVEWMKGTGVKVLNLLTKGSLNHLTNQLRKTP
HHHHHHHHHHHCCCCCHHHEEEEECHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCH
EEHFNDIKQTVEVAVKAGITVNVYLEDWSNGYLHSRDYVIQYLKTVSEFPIKRFYLADTL
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEECHHHHHHHHHHHHHCCHHHHHHHHHH
GVMSPEEVKKAVTDLVSLFPNLWFEFHGHNDYDLAVANCLEAVKAGARGLHVAVNGLGER
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHC
AGNSPLEAVVTSLHDKTKFRTSIVEKEITNASRLVEVFSGKRISDNRPIVGEDVFTQTAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHCCC
VHADGDKKGNLYANPILPERFGRSRVYALGKLAGKASITENLKQLGMVLSPEIEKKVLER
CCCCCCCCCCEEECCCCHHHHCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHH
VIELGDQNKTVTKEDLPYIISDISGENLESSFRIEFCTVTSGIGVKPTADVKVKFQGKLL
HHHCCCCCCCCCHHCCCHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEECEEE
EAKGEGDGGYDAFMNALGKILETLKIQIPKLYDYEVRIPPGGNTNALVETVITWKKDADI
EECCCCCCCHHHHHHHHHHHHHHHHHCCCCEECEEEEECCCCCCHHHHHHHHHHCCCCCC
HPIRTIGIDSDQQVAAVKATERMLHIVLGNL
CCEEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]